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The map label for this gene is ilvB [H]

Identifier: 138894022

GI number: 138894022

Start: 397108

End: 398763

Strand: Direct

Name: ilvB [H]

Synonym: GTNG_0348

Alternate gene names: 138894022

Gene position: 397108-398763 (Clockwise)

Preceding gene: 138894021

Following gene: 138894023

Centisome position: 11.19

GC content: 57.13

Gene sequence:

>1656_bases
ATGGGAGGGACGGCCATGAAAAAGCGGGTGATGCGTGGCCTTTCTGTCCCGCAAGCGATTGTCGAATGCTTAAAACAAGA
GCGTATTTCTCATTTGTTTGGCGTGCCGGGAGAAAGTTATTTGCCGCTGTTGGATGCGTTGTACGATGAACCAACCCTTC
AGTTCATTTCTGCCCGCCATGAAGGAGGGGCGGCGTTTATGGCTGAAGCGTATGCGAAAGCGTCCGGCAAACCAGGTGTC
GTCTTAGCGACGCGCGGAGTCGGAGCGTCTAATTTGGCCATCGGGGTTCATACAGCACGTCAAGATTCGACCCCACTTGT
CGTCCTGTTAGGACAAGTGCATCGCCGATTCCGTGGTCGTGAGGGATTTCAAGAAGTAGAACTAGACGAATGGTTTCGGC
CGCTTGCGAAATGGGCAGTCGAAATCACCGACCCCGAGCGCGTTCCAGAACTCGTGCAACGGGCATTTCGGGTGGCGAAA
ACGGGACGACCTGGTCCGGTTGTCGTCTCTATTCCTGAGGATGTGTTTGCGTCAACGGTTGCTGAGGCGGTGTTGATGAC
GATGGATGTGCCGCGCCCAGCCCCAGCGGATGATGAGGTGAAGCGGATTGAAGCGGCACTCGCTGCTGCCCGTCGGCCGC
TCATTGTCGCCGGTGGCGGGGTGAAGCGGGGGCGCGCTGAGCAGGCGCTGCGTCGCGTCGCTGAACAATATTCCCTTCCG
GTTGTTGCCGCATTCCGCCGCCATGACGTGTTTCCGCATGACCATCCGCTTTATGTCGGCCATCTCGGTCTTGGCGCACC
GGCAGCTGTCGTTGAGACGGCCAAGCAGGCGGATGTTATTTTGGCGGTTGGAACGCGGTTGGCGGAAGTAACAACGCAAG
ATTATACATGGCCGCTGCCGCATCAGATGCTCATCCATATCGATATCGACGAGGAGGCGCTTGGCAAAGTGTTTTCAGCG
GACATCGCCGTTGCGGCCGATGCCCACGAGGCGTTGTGTGCACTGTTAGGGCGGCATATCGTCCCGACGTGGAATGAATG
GGTCTTGGAGCGCCGACAAGCTTATGAAGAAACAGCGAGGCTGCCGCAGCCGCCAAGAAACGTGCAAGAAGCCATCATCG
CTCAGCTACAAGCGCAGTTGCCGGACGATGGAATGATTACGAACGATGCCGGCAATTTTGCCGGCTGGCTGCACACCTTT
TTCTCCTTCAAGGAACAGCATATGTATATTGGCCCAACATCAGGAGCGATGGGATACGGGCTGCCGGCGGCCATCGGCGC
GAAGCTCGCCCATCCGGAACGGCCGGCCGTTTCGTTGTCAGGAGATGGTGGGTTTCTCATGACGGCGGCCGAACTCGAGA
CGGCGTCCCGTTATGGCGTCCCGGTCATCAGTCTCGTGTTTAACAACCGGATGTATGGGACTATTCGTATGTATCAAGAG
CTTCAGTTCCCAGGACGAGTGGTCGGCAGCGGACTAGGTGAAGTGTCGTTTGCCAGATTGGCTAGGGCATTAGGTGCCAA
CGGTGTTACGGTGGAGACAGCCGATGAGTTTGCCGCTGCCTTTAAGCAGGCGCTCACTGCGACGAAACCAACCGTGATTG
AGGTGATGACGGAGCCGGAACAACTGTCTGTGACGATGAGACTGTCAGACAGATGA

Upstream 100 bases:

>100_bases
GGACGGCGTTTGGTTCAGCAGGGGAAGGGTATGTGCGCATATCACTCGCCGCTCCGAAGGAACGGCTGCTCGAAGCCGTT
CGGCGCTGGAAAGCGATTGA

Downstream 100 bases:

>100_bases
AAACATTGGAGAAGAGTTTCCGCATTGAGACCGAGATAGCCCATGAATCATGCAATGAAAAGGACAGCTGCAGCAAAGTA
ACAGCGAAACGACATCTACT

Product: thiamine pyrophosphate protein

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 551; Mature: 550

Protein sequence:

>551_residues
MGGTAMKKRVMRGLSVPQAIVECLKQERISHLFGVPGESYLPLLDALYDEPTLQFISARHEGGAAFMAEAYAKASGKPGV
VLATRGVGASNLAIGVHTARQDSTPLVVLLGQVHRRFRGREGFQEVELDEWFRPLAKWAVEITDPERVPELVQRAFRVAK
TGRPGPVVVSIPEDVFASTVAEAVLMTMDVPRPAPADDEVKRIEAALAAARRPLIVAGGGVKRGRAEQALRRVAEQYSLP
VVAAFRRHDVFPHDHPLYVGHLGLGAPAAVVETAKQADVILAVGTRLAEVTTQDYTWPLPHQMLIHIDIDEEALGKVFSA
DIAVAADAHEALCALLGRHIVPTWNEWVLERRQAYEETARLPQPPRNVQEAIIAQLQAQLPDDGMITNDAGNFAGWLHTF
FSFKEQHMYIGPTSGAMGYGLPAAIGAKLAHPERPAVSLSGDGGFLMTAAELETASRYGVPVISLVFNNRMYGTIRMYQE
LQFPGRVVGSGLGEVSFARLARALGANGVTVETADEFAAAFKQALTATKPTVIEVMTEPEQLSVTMRLSDR

Sequences:

>Translated_551_residues
MGGTAMKKRVMRGLSVPQAIVECLKQERISHLFGVPGESYLPLLDALYDEPTLQFISARHEGGAAFMAEAYAKASGKPGV
VLATRGVGASNLAIGVHTARQDSTPLVVLLGQVHRRFRGREGFQEVELDEWFRPLAKWAVEITDPERVPELVQRAFRVAK
TGRPGPVVVSIPEDVFASTVAEAVLMTMDVPRPAPADDEVKRIEAALAAARRPLIVAGGGVKRGRAEQALRRVAEQYSLP
VVAAFRRHDVFPHDHPLYVGHLGLGAPAAVVETAKQADVILAVGTRLAEVTTQDYTWPLPHQMLIHIDIDEEALGKVFSA
DIAVAADAHEALCALLGRHIVPTWNEWVLERRQAYEETARLPQPPRNVQEAIIAQLQAQLPDDGMITNDAGNFAGWLHTF
FSFKEQHMYIGPTSGAMGYGLPAAIGAKLAHPERPAVSLSGDGGFLMTAAELETASRYGVPVISLVFNNRMYGTIRMYQE
LQFPGRVVGSGLGEVSFARLARALGANGVTVETADEFAAAFKQALTATKPTVIEVMTEPEQLSVTMRLSDR
>Mature_550_residues
GGTAMKKRVMRGLSVPQAIVECLKQERISHLFGVPGESYLPLLDALYDEPTLQFISARHEGGAAFMAEAYAKASGKPGVV
LATRGVGASNLAIGVHTARQDSTPLVVLLGQVHRRFRGREGFQEVELDEWFRPLAKWAVEITDPERVPELVQRAFRVAKT
GRPGPVVVSIPEDVFASTVAEAVLMTMDVPRPAPADDEVKRIEAALAAARRPLIVAGGGVKRGRAEQALRRVAEQYSLPV
VAAFRRHDVFPHDHPLYVGHLGLGAPAAVVETAKQADVILAVGTRLAEVTTQDYTWPLPHQMLIHIDIDEEALGKVFSAD
IAVAADAHEALCALLGRHIVPTWNEWVLERRQAYEETARLPQPPRNVQEAIIAQLQAQLPDDGMITNDAGNFAGWLHTFF
SFKEQHMYIGPTSGAMGYGLPAAIGAKLAHPERPAVSLSGDGGFLMTAAELETASRYGVPVISLVFNNRMYGTIRMYQEL
QFPGRVVGSGLGEVSFARLARALGANGVTVETADEFAAAFKQALTATKPTVIEVMTEPEQLSVTMRLSDR

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=581, Percent_Identity=25.8175559380379, Blast_Score=166, Evalue=6e-41,
Organism=Homo sapiens, GI21361361, Length=583, Percent_Identity=25.3859348198971, Blast_Score=142, Evalue=1e-33,
Organism=Escherichia coli, GI87081685, Length=537, Percent_Identity=32.4022346368715, Blast_Score=279, Evalue=4e-76,
Organism=Escherichia coli, GI1790104, Length=550, Percent_Identity=30.5454545454545, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI1786717, Length=551, Percent_Identity=30.8529945553539, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1787096, Length=543, Percent_Identity=27.255985267035, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI1788716, Length=553, Percent_Identity=26.7631103074141, Blast_Score=141, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17542570, Length=567, Percent_Identity=23.4567901234568, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17531299, Length=562, Percent_Identity=25.6227758007117, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17531301, Length=562, Percent_Identity=25.6227758007117, Blast_Score=128, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6323755, Length=558, Percent_Identity=30.8243727598566, Blast_Score=256, Evalue=7e-69,
Organism=Saccharomyces cerevisiae, GI6320816, Length=560, Percent_Identity=25, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6320123, Length=582, Percent_Identity=21.9931271477663, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323073, Length=506, Percent_Identity=20.7509881422925, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323163, Length=497, Percent_Identity=20.523138832998, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321524, Length=515, Percent_Identity=20.5825242718447, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI19922626, Length=566, Percent_Identity=27.7385159010601, Blast_Score=163, Evalue=3e-40,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59774; Mature: 59643

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGTAMKKRVMRGLSVPQAIVECLKQERISHLFGVPGESYLPLLDALYDEPTLQFISARH
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHC
EGGAAFMAEAYAKASGKPGVVLATRGVGASNLAIGVHTARQDSTPLVVLLGQVHRRFRGR
CCCHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCC
EGFQEVELDEWFRPLAKWAVEITDPERVPELVQRAFRVAKTGRPGPVVVSIPEDVFASTV
CCCHHCCHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHH
AEAVLMTMDVPRPAPADDEVKRIEAALAAARRPLIVAGGGVKRGRAEQALRRVAEQYSLP
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCC
VVAAFRRHDVFPHDHPLYVGHLGLGAPAAVVETAKQADVILAVGTRLAEVTTQDYTWPLP
HHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCEEEEECCHHHHHCCCCCCCCCC
HQMLIHIDIDEEALGKVFSADIAVAADAHEALCALLGRHIVPTWNEWVLERRQAYEETAR
CEEEEEEECCHHHHHHHHHCCCEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHC
LPQPPRNVQEAIIAQLQAQLPDDGMITNDAGNFAGWLHTFFSFKEQHMYIGPTSGAMGYG
CCCCCHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHCEEEECCCCCCCCCC
LPAAIGAKLAHPERPAVSLSGDGGFLMTAAELETASRYGVPVISLVFNNRMYGTIRMYQE
CHHHHHHHCCCCCCCEEEECCCCCEEEEHHHHHHHHHCCCCEEEEECCCCCEEHHHHHHH
LQFPGRVVGSGLGEVSFARLARALGANGVTVETADEFAAAFKQALTATKPTVIEVMTEPE
HCCCCHHHHCCCCHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHCCCCEEEEEECCCC
QLSVTMRLSDR
CEEEEEEECCC
>Mature Secondary Structure 
GGTAMKKRVMRGLSVPQAIVECLKQERISHLFGVPGESYLPLLDALYDEPTLQFISARH
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHC
EGGAAFMAEAYAKASGKPGVVLATRGVGASNLAIGVHTARQDSTPLVVLLGQVHRRFRGR
CCCHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCC
EGFQEVELDEWFRPLAKWAVEITDPERVPELVQRAFRVAKTGRPGPVVVSIPEDVFASTV
CCCHHCCHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHH
AEAVLMTMDVPRPAPADDEVKRIEAALAAARRPLIVAGGGVKRGRAEQALRRVAEQYSLP
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCC
VVAAFRRHDVFPHDHPLYVGHLGLGAPAAVVETAKQADVILAVGTRLAEVTTQDYTWPLP
HHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCEEEEECCHHHHHCCCCCCCCCC
HQMLIHIDIDEEALGKVFSADIAVAADAHEALCALLGRHIVPTWNEWVLERRQAYEETAR
CEEEEEEECCHHHHHHHHHCCCEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHC
LPQPPRNVQEAIIAQLQAQLPDDGMITNDAGNFAGWLHTFFSFKEQHMYIGPTSGAMGYG
CCCCCHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHCEEEECCCCCCCCCC
LPAAIGAKLAHPERPAVSLSGDGGFLMTAAELETASRYGVPVISLVFNNRMYGTIRMYQE
CHHHHHHHCCCCCCCEEEECCCCCEEEEHHHHHHHHHCCCCEEEEECCCCCEEHHHHHHH
LQFPGRVVGSGLGEVSFARLARALGANGVTVETADEFAAAFKQALTATKPTVIEVMTEPE
HCCCCHHHHCCCCHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHCCCCEEEEEECCCC
QLSVTMRLSDR
CEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]