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The map label for this gene is purD [H]

Identifier: 138893924

GI number: 138893924

Start: 276679

End: 277971

Strand: Direct

Name: purD [H]

Synonym: GTNG_0248

Alternate gene names: 138893924

Gene position: 276679-277971 (Clockwise)

Preceding gene: 138893923

Following gene: 138893926

Centisome position: 7.79

GC content: 55.92

Gene sequence:

>1293_bases
ATGAACATTCTCGTAGTCGGACGCGGCGGGCGTGAGCATGCGATCGCCTGGAAGGTGGCGCAAAGCCCGCTTGCTGGCAA
GCTGTATGCGGCGCCAGGCAACCCGGGCATCGGGGAAGTGGCGGAGTTGGTAAATATCGATGAACTCGATATTGATGCGC
TCGTGCAGTTTGCGAAACAAAACGCGATTGATTTGACGATTGTCGGACCGGAGGCGCCGCTTGCCGCCGGCATTGCCGAC
CGTTTTATGGCTGAGGGGCTGCGCATTTTTGGACCGAACCAAGCAGCGGCACTCATTGAAGGGAGCAAAGCGTTCGCGAA
AGAGCTCATGAAAACATACGGCATTCCGACGGCGGAACATGCTACCTTTTCTTCGTACGAAGAAGCCAAAGCGTATATTG
AGGAAAAAGGAGCGCCGATCGTCATTAAAGCGGACGGGTTGGCAGCTGGAAAAGGAGTCACTGTCGCCCAAACGGTGGAA
GAGGCGCTTGCCGCAGCAAAAGCAGCACTGGTGGACGGGCAATTCGGCACAGCCGGCAGCCAAGTCGTGATCGAGGAGTA
TTTGGAAGGCGAAGAATTTTCATTTATGGCGTTCGTGAACGGTGAGAAGGTGTATCCTCTTGCTATTGCCCAAGACCATA
AACGAGCCTATGACGGGGACGAAGGGCCAAACACTGGTGGAATGGGAGCCTATTCGCCAGTGCCGCAAATTTCCGCTGAT
ATGGTTCAACGGGCGTTGGAGACGATTTTGCGTCCGACCGCCAAAGCGTTAGCTTCCGAAGGCCGGCCGTTTACCGGTGT
GCTGTATGCCGGGTTGATTGCCACTGCGGAAGGACCGAAAGTGATCGAATTTAACGCCCGATTCGGTGACCCAGAGGCGC
AAGTCGTGTTGCCGCGCCTAAAAACCGATTTGCTTGAAGCGATATTGGCGGTGATGGAAGGAAAAGAGCTGGAGTTGGAG
TGGACGAATGAAGCAGTGCTTGGCGTCGTGCTGGCAGCTAAAGGTTATCCGGGTGCTTACGAGCGCGGAGTGGCCATCCG
CGGACTTGACCGGCTCGCTCCGGATGCGTTGTTGTTCCATGCTGGTACGAAGCGGGAAGGCGGAACGCTCTATACAAACG
GCGGCCGTGTCCTTTTGTTAGCAGCCAAAGGAGGAACGCTTGCTGAAGCAAAGGAAAAAGCGTACCAGCAGCTTGCGGCG
ATCGAATGTGATGGCCTGTTTTACCGCCGTGACATTGGCCGGCGCGCTATCGAACGCGCCTCCGCCGTAGATACACGTAT
GAAAGGGCGATAA

Upstream 100 bases:

>100_bases
CTGATTCGATCCGCAAAGCAAATGAGTACGGCATTGCCATGGTGTTTACTGGTGTTCGCCATTTCAAACATTAAACGGCG
GCAAAAAGGGGAGGAAGATG

Downstream 100 bases:

>100_bases
TACCGCCGATCAATGTTGCTAATACAAACGACATATCGGTCATATATTCAAGCAACATCATCATTCACTCCTTTGTATCG
AAGGTGTCCCAACCAAACGT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MNILVVGRGGREHAIAWKVAQSPLAGKLYAAPGNPGIGEVAELVNIDELDIDALVQFAKQNAIDLTIVGPEAPLAAGIAD
RFMAEGLRIFGPNQAAALIEGSKAFAKELMKTYGIPTAEHATFSSYEEAKAYIEEKGAPIVIKADGLAAGKGVTVAQTVE
EALAAAKAALVDGQFGTAGSQVVIEEYLEGEEFSFMAFVNGEKVYPLAIAQDHKRAYDGDEGPNTGGMGAYSPVPQISAD
MVQRALETILRPTAKALASEGRPFTGVLYAGLIATAEGPKVIEFNARFGDPEAQVVLPRLKTDLLEAILAVMEGKELELE
WTNEAVLGVVLAAKGYPGAYERGVAIRGLDRLAPDALLFHAGTKREGGTLYTNGGRVLLLAAKGGTLAEAKEKAYQQLAA
IECDGLFYRRDIGRRAIERASAVDTRMKGR

Sequences:

>Translated_430_residues
MNILVVGRGGREHAIAWKVAQSPLAGKLYAAPGNPGIGEVAELVNIDELDIDALVQFAKQNAIDLTIVGPEAPLAAGIAD
RFMAEGLRIFGPNQAAALIEGSKAFAKELMKTYGIPTAEHATFSSYEEAKAYIEEKGAPIVIKADGLAAGKGVTVAQTVE
EALAAAKAALVDGQFGTAGSQVVIEEYLEGEEFSFMAFVNGEKVYPLAIAQDHKRAYDGDEGPNTGGMGAYSPVPQISAD
MVQRALETILRPTAKALASEGRPFTGVLYAGLIATAEGPKVIEFNARFGDPEAQVVLPRLKTDLLEAILAVMEGKELELE
WTNEAVLGVVLAAKGYPGAYERGVAIRGLDRLAPDALLFHAGTKREGGTLYTNGGRVLLLAAKGGTLAEAKEKAYQQLAA
IECDGLFYRRDIGRRAIERASAVDTRMKGR
>Mature_430_residues
MNILVVGRGGREHAIAWKVAQSPLAGKLYAAPGNPGIGEVAELVNIDELDIDALVQFAKQNAIDLTIVGPEAPLAAGIAD
RFMAEGLRIFGPNQAAALIEGSKAFAKELMKTYGIPTAEHATFSSYEEAKAYIEEKGAPIVIKADGLAAGKGVTVAQTVE
EALAAAKAALVDGQFGTAGSQVVIEEYLEGEEFSFMAFVNGEKVYPLAIAQDHKRAYDGDEGPNTGGMGAYSPVPQISAD
MVQRALETILRPTAKALASEGRPFTGVLYAGLIATAEGPKVIEFNARFGDPEAQVVLPRLKTDLLEAILAVMEGKELELE
WTNEAVLGVVLAAKGYPGAYERGVAIRGLDRLAPDALLFHAGTKREGGTLYTNGGRVLLLAAKGGTLAEAKEKAYQQLAA
IECDGLFYRRDIGRRAIERASAVDTRMKGR

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=422, Percent_Identity=48.1042654028436, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI209869995, Length=422, Percent_Identity=48.1042654028436, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI209869993, Length=422, Percent_Identity=48.1042654028436, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI28416899, Length=422, Percent_Identity=48.1042654028436, Blast_Score=385, Evalue=1e-107,
Organism=Escherichia coli, GI1790438, Length=426, Percent_Identity=55.1643192488263, Blast_Score=439, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17567511, Length=423, Percent_Identity=40.1891252955083, Blast_Score=305, Evalue=3e-83,
Organism=Saccharomyces cerevisiae, GI6321203, Length=445, Percent_Identity=40.6741573033708, Blast_Score=323, Evalue=3e-89,
Organism=Drosophila melanogaster, GI24582400, Length=436, Percent_Identity=44.7247706422018, Blast_Score=325, Evalue=4e-89,
Organism=Drosophila melanogaster, GI62471619, Length=432, Percent_Identity=44.4444444444444, Blast_Score=322, Evalue=2e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45501; Mature: 45501

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILVVGRGGREHAIAWKVAQSPLAGKLYAAPGNPGIGEVAELVNIDELDIDALVQFAKQ
CEEEEEECCCCCCEEEEEECCCCCCCEEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHC
NAIDLTIVGPEAPLAAGIADRFMAEGLRIFGPNQAAALIEGSKAFAKELMKTYGIPTAEH
CCEEEEEECCCCCHHHHHHHHHHHCCCEEECCCCCEEEEECCHHHHHHHHHHHCCCCCCC
ATFSSYEEAKAYIEEKGAPIVIKADGLAAGKGVTVAQTVEEALAAAKAALVDGQFGTAGS
CCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
QVVIEEYLEGEEFSFMAFVNGEKVYPLAIAQDHKRAYDGDEGPNTGGMGAYSPVPQISAD
HHHHHHHHCCCCEEEEEEECCCEEEEEEEECCHHHCCCCCCCCCCCCCCCCCCCCCHHHH
MVQRALETILRPTAKALASEGRPFTGVLYAGLIATAEGPKVIEFNARFGDPEAQVVLPRL
HHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHEECCCCEEEEEECCCCCCCCEEEHHHH
KTDLLEAILAVMEGKELELEWTNEAVLGVVLAAKGYPGAYERGVAIRGLDRLAPDALLFH
HHHHHHHHHHHHCCCEEEEEECCCCEEEEEEEECCCCCHHHCCEEEECHHHCCCCEEEEE
AGTKREGGTLYTNGGRVLLLAAKGGTLAEAKEKAYQQLAAIECDGLFYRRDIGRRAIERA
CCCCCCCCEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
SAVDTRMKGR
HHHHHHCCCC
>Mature Secondary Structure
MNILVVGRGGREHAIAWKVAQSPLAGKLYAAPGNPGIGEVAELVNIDELDIDALVQFAKQ
CEEEEEECCCCCCEEEEEECCCCCCCEEEECCCCCCHHHHHHHHCCCCCCHHHHHHHHHC
NAIDLTIVGPEAPLAAGIADRFMAEGLRIFGPNQAAALIEGSKAFAKELMKTYGIPTAEH
CCEEEEEECCCCCHHHHHHHHHHHCCCEEECCCCCEEEEECCHHHHHHHHHHHCCCCCCC
ATFSSYEEAKAYIEEKGAPIVIKADGLAAGKGVTVAQTVEEALAAAKAALVDGQFGTAGS
CCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
QVVIEEYLEGEEFSFMAFVNGEKVYPLAIAQDHKRAYDGDEGPNTGGMGAYSPVPQISAD
HHHHHHHHCCCCEEEEEEECCCEEEEEEEECCHHHCCCCCCCCCCCCCCCCCCCCCHHHH
MVQRALETILRPTAKALASEGRPFTGVLYAGLIATAEGPKVIEFNARFGDPEAQVVLPRL
HHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHEECCCCEEEEEECCCCCCCCEEEHHHH
KTDLLEAILAVMEGKELELEWTNEAVLGVVLAAKGYPGAYERGVAIRGLDRLAPDALLFH
HHHHHHHHHHHHCCCEEEEEECCCCEEEEEEEECCCCCHHHCCEEEECHHHCCCCEEEEE
AGTKREGGTLYTNGGRVLLLAAKGGTLAEAKEKAYQQLAAIECDGLFYRRDIGRRAIERA
CCCCCCCCEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
SAVDTRMKGR
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]