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The map label for this gene is purF [H]

Identifier: 138893920

GI number: 138893920

Start: 271972

End: 273384

Strand: Direct

Name: purF [H]

Synonym: GTNG_0244

Alternate gene names: 138893920

Gene position: 271972-273384 (Clockwise)

Preceding gene: 138893919

Following gene: 138893921

Centisome position: 7.66

GC content: 55.91

Gene sequence:

>1413_bases
ATGCTTGCTGAAATCAAAGGATTGAACGAGGAGTGCGGCATTTTCGGCATTTGGGGGCATGAGGACGCCGCTAGGCTTAC
CTATTACGGGCTCCACAGCTTGCAGCACCGCGGCCAAGAAGGAGCGGGCATCGTGGTGGCGAGCGGCGGAAACTTATTCG
GTCATAAAGGACTCGGTTTGGTGACAGAGGTGTTTCAAAGCGACACGTTTGATGCGCTTCACGGGTCGGCGGCGATCGGC
CATGTCCGCTATTCGACGGCAGGCGGGGGCGGCTATGAGAACGTCCAGCCGCTCCTGTTCCGCTCACAGACTGGCTCGAT
GGCGCTCGCCCATAACGGCAACTTGACGAACGCTATCGATCTGAAGCTCTCGCTCGAAGCGCAAGGAAGCATTTTTCAGA
CGACATCGGATACGGAAGTGTTCGCCCATCTTATCCGTCGCAGCCAAGCGCCAACGTTTGTCGGACAAATGAAAGAGGCG
CTCAGCCAGATTGAAGGAGCGTTTGCGTTTTTATTGCTGACGGAAACGGCGCTTTATGTGGCGCTCGACCCGCACGGATT
CCGGCCATTGTCACTCGGCCGGCTCGGATCAGCGTATGTCGTCGCCTCGGAAACGTGCGCGTTTGATGTCATCGGGGCGA
CGTACGAGCGCGAAGTGGCTCCGGGGGAACTGCTTATCATCAGCGGCGAAGGGGTGCGTTCGGAACGATTTGCCCCGGAG
CAGCCGCGATCCATTTGCAGCATGGAATACATTTATTTTGCTCGTCCGGACAGCCATGTTGATGGCATCAACATCCATAC
AGCCCGGAAAAATTTAGGGAAACAGTTGGCACTTGAGGCGCCAGCTGAAGCCGATATTGTCACTGGGGTGCCGGACTCAA
GCATTTCCGTCGCTATCGGTTATGCCGAAGCGAGTGGCATTCCGTACGAATTGGGGCTCATTAAAAACCGTTATGTAGGT
CGGACGTTTATTCAGCCGTCACAAGCGTTGCGTGAACAAGGGGTAAAAATGAAGCTGTCGCCGGTGCGCGGTGTTGTCGC
TGGCAAGCGGGTTGTCATGGTCGACGATTCGATCGTGCGCGGGACGACGAGCCGCCGCATTGTCACAATGCTTCGCGAAG
CGGGAGCTTTGGAAGTGCATGTGCGTATCAGTGCGCCGCCGATCACCCATCCTTGCTTTTACGGCATTGATACGTCGTCA
AAGGAAGAACTGATTGCGGCGAAACATACGGTTGAAGACATCTGCCGCTTAATCGGCGCCGATTCGTTGGCGTTTATTAG
CCAGGAAGGGCTGCTGTCAGCGATCGGTCGTCCTGAAGGCATTCCACATCGCGGACAATGTTTGGCTTGCTTTACCGGCC
AATACCCAACCCGGGTCAACCAAACTACTCGGCCTTGCATGACAGTCAAATGA

Upstream 100 bases:

>100_bases
TGACCAATGACAGCATACTGACAGTAAACGGGGAACATGGGGGAACGGTTATTCGCCTTTCGGTCGACGAAATGCGAAAC
GTCTGGAAAGGGGCTATTCC

Downstream 100 bases:

>100_bases
TCAATGATCAGCGTGCTTCAAGTTGGAAGGGGACAACGGACAGCAAGGTGCTTGTCGCATGCTTGGGTGCAGCGGGCGAA
GAAAAGGGGGATTTAGCGTG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MLAEIKGLNEECGIFGIWGHEDAARLTYYGLHSLQHRGQEGAGIVVASGGNLFGHKGLGLVTEVFQSDTFDALHGSAAIG
HVRYSTAGGGGYENVQPLLFRSQTGSMALAHNGNLTNAIDLKLSLEAQGSIFQTTSDTEVFAHLIRRSQAPTFVGQMKEA
LSQIEGAFAFLLLTETALYVALDPHGFRPLSLGRLGSAYVVASETCAFDVIGATYEREVAPGELLIISGEGVRSERFAPE
QPRSICSMEYIYFARPDSHVDGINIHTARKNLGKQLALEAPAEADIVTGVPDSSISVAIGYAEASGIPYELGLIKNRYVG
RTFIQPSQALREQGVKMKLSPVRGVVAGKRVVMVDDSIVRGTTSRRIVTMLREAGALEVHVRISAPPITHPCFYGIDTSS
KEELIAAKHTVEDICRLIGADSLAFISQEGLLSAIGRPEGIPHRGQCLACFTGQYPTRVNQTTRPCMTVK

Sequences:

>Translated_470_residues
MLAEIKGLNEECGIFGIWGHEDAARLTYYGLHSLQHRGQEGAGIVVASGGNLFGHKGLGLVTEVFQSDTFDALHGSAAIG
HVRYSTAGGGGYENVQPLLFRSQTGSMALAHNGNLTNAIDLKLSLEAQGSIFQTTSDTEVFAHLIRRSQAPTFVGQMKEA
LSQIEGAFAFLLLTETALYVALDPHGFRPLSLGRLGSAYVVASETCAFDVIGATYEREVAPGELLIISGEGVRSERFAPE
QPRSICSMEYIYFARPDSHVDGINIHTARKNLGKQLALEAPAEADIVTGVPDSSISVAIGYAEASGIPYELGLIKNRYVG
RTFIQPSQALREQGVKMKLSPVRGVVAGKRVVMVDDSIVRGTTSRRIVTMLREAGALEVHVRISAPPITHPCFYGIDTSS
KEELIAAKHTVEDICRLIGADSLAFISQEGLLSAIGRPEGIPHRGQCLACFTGQYPTRVNQTTRPCMTVK
>Mature_470_residues
MLAEIKGLNEECGIFGIWGHEDAARLTYYGLHSLQHRGQEGAGIVVASGGNLFGHKGLGLVTEVFQSDTFDALHGSAAIG
HVRYSTAGGGGYENVQPLLFRSQTGSMALAHNGNLTNAIDLKLSLEAQGSIFQTTSDTEVFAHLIRRSQAPTFVGQMKEA
LSQIEGAFAFLLLTETALYVALDPHGFRPLSLGRLGSAYVVASETCAFDVIGATYEREVAPGELLIISGEGVRSERFAPE
QPRSICSMEYIYFARPDSHVDGINIHTARKNLGKQLALEAPAEADIVTGVPDSSISVAIGYAEASGIPYELGLIKNRYVG
RTFIQPSQALREQGVKMKLSPVRGVVAGKRVVMVDDSIVRGTTSRRIVTMLREAGALEVHVRISAPPITHPCFYGIDTSS
KEELIAAKHTVEDICRLIGADSLAFISQEGLLSAIGRPEGIPHRGQCLACFTGQYPTRVNQTTRPCMTVK

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=517, Percent_Identity=39.458413926499, Blast_Score=361, Evalue=1e-100,
Organism=Homo sapiens, GI4826742, Length=187, Percent_Identity=27.807486631016, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788651, Length=470, Percent_Identity=40, Blast_Score=305, Evalue=6e-84,
Organism=Escherichia coli, GI1790167, Length=200, Percent_Identity=29.5, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17554892, Length=482, Percent_Identity=40.8713692946058, Blast_Score=347, Evalue=7e-96,
Organism=Saccharomyces cerevisiae, GI6323958, Length=452, Percent_Identity=35.6194690265487, Blast_Score=247, Evalue=2e-66,
Organism=Drosophila melanogaster, GI28573187, Length=496, Percent_Identity=40.9274193548387, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24659598, Length=486, Percent_Identity=39.7119341563786, Blast_Score=349, Evalue=2e-96,
Organism=Drosophila melanogaster, GI24659604, Length=457, Percent_Identity=40.7002188183807, Blast_Score=342, Evalue=3e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 50542; Mature: 50542

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAEIKGLNEECGIFGIWGHEDAARLTYYGLHSLQHRGQEGAGIVVASGGNLFGHKGLGL
CCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCEECCCCCHH
VTEVFQSDTFDALHGSAAIGHVRYSTAGGGGYENVQPLLFRSQTGSMALAHNGNLTNAID
HHHHHHCCCHHHHCCCEEEEEEEEECCCCCCCCCCCEEEEECCCCCEEEEECCCCCEEEE
LKLSLEAQGSIFQTTSDTEVFAHLIRRSQAPTFVGQMKEALSQIEGAFAFLLLTETALYV
EEEEEECCCCEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEEEECCEEEE
ALDPHGFRPLSLGRLGSAYVVASETCAFDVIGATYEREVAPGELLIISGEGVRSERFAPE
EECCCCCCEEECCCCCCEEEEEECCCEEEEECCCHHCCCCCCCEEEECCCCCCCCCCCCC
QPRSICSMEYIYFARPDSHVDGINIHTARKNLGKQLALEAPAEADIVTGVPDSSISVAIG
CCHHHHCEEEEEEECCCCCCCCEEEEHHHHHCCCEEEECCCCCCCEEECCCCCCEEEEEE
YAEASGIPYELGLIKNRYVGRTFIQPSQALREQGVKMKLSPVRGVVAGKRVVMVDDSIVR
EECCCCCCEEEEECCCCCCCCEECCHHHHHHHCCCEEEECCCCHHHCCCEEEEECCHHHC
GTTSRRIVTMLREAGALEVHVRISAPPITHPCFYGIDTSSKEELIAAKHTVEDICRLIGA
CCCHHHHHHHHHHCCCEEEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCC
DSLAFISQEGLLSAIGRPEGIPHRGQCLACFTGQYPTRVNQTTRPCMTVK
CCEEEHHHCCHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEECC
>Mature Secondary Structure
MLAEIKGLNEECGIFGIWGHEDAARLTYYGLHSLQHRGQEGAGIVVASGGNLFGHKGLGL
CCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCEECCCCCHH
VTEVFQSDTFDALHGSAAIGHVRYSTAGGGGYENVQPLLFRSQTGSMALAHNGNLTNAID
HHHHHHCCCHHHHCCCEEEEEEEEECCCCCCCCCCCEEEEECCCCCEEEEECCCCCEEEE
LKLSLEAQGSIFQTTSDTEVFAHLIRRSQAPTFVGQMKEALSQIEGAFAFLLLTETALYV
EEEEEECCCCEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEEEECCEEEE
ALDPHGFRPLSLGRLGSAYVVASETCAFDVIGATYEREVAPGELLIISGEGVRSERFAPE
EECCCCCCEEECCCCCCEEEEEECCCEEEEECCCHHCCCCCCCEEEECCCCCCCCCCCCC
QPRSICSMEYIYFARPDSHVDGINIHTARKNLGKQLALEAPAEADIVTGVPDSSISVAIG
CCHHHHCEEEEEEECCCCCCCCEEEEHHHHHCCCEEEECCCCCCCEEECCCCCCEEEEEE
YAEASGIPYELGLIKNRYVGRTFIQPSQALREQGVKMKLSPVRGVVAGKRVVMVDDSIVR
EECCCCCCEEEEECCCCCCCCEECCHHHHHHHCCCEEEECCCCHHHCCCEEEEECCHHHC
GTTSRRIVTMLREAGALEVHVRISAPPITHPCFYGIDTSSKEELIAAKHTVEDICRLIGA
CCCHHHHHHHHHHCCCEEEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCC
DSLAFISQEGLLSAIGRPEGIPHRGQCLACFTGQYPTRVNQTTRPCMTVK
CCEEEHHHCCHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]