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The map label for this gene is cwlD [H]

Identifier: 138893819

GI number: 138893819

Start: 145485

End: 146201

Strand: Direct

Name: cwlD [H]

Synonym: GTNG_0140

Alternate gene names: 138893819

Gene position: 145485-146201 (Clockwise)

Preceding gene: 138893818

Following gene: 138893820

Centisome position: 4.1

GC content: 51.46

Gene sequence:

>717_bases
ATGAAAAAAAAGTGGAAGTGGCTGGTCGCTGTTGCTGCGATCACCATCCTCGGTGTTTTGTTATTTCCATCCTTATTTTC
TGATCTTACATCGACCAAGCCTTGGAATCTTCCGTTATCAGGGAGAATTATCGTATTAGACCCCGGCCACGGTGGACCAG
ACGGCGGTGCTGTTGGCGGCGAGGTGGTAGAAAAGGAAATCGCGCTGAACGTGGCGAAAAAACTGCGCGATTACTTGCAG
CAGCAAGGCGCGCTCGTGCTTATGACACGAGAGACGGATCGCGACTTGGCCAGCCCGTCAACGCGCGGCTACAGCCGGCG
GAAAACGGAAGACTTGCGTGAACGAACGACATTCATTAACAAGTCCGATGCGGATCTATTCATCAGCATTCATTTAAACG
CCATTCCATCGCCGCGATGGCGGGGGGCACAGACGTTTTACTACGGTTCGCTCATCGAAAACGAGCGGCTGGCGAAATTT
ATCCAGGCTGAGCTACGGCGCAACTTGGAAAACACTCATCGGGTGGCGAAAATGATTGACACGGTTTATTTGTTGAAACA
CGCGAAAAAGCCAGGTGCGCTTGTTGAGATCGGGTTTTTATCGAATCCGGACGAGCGGGAATTGCTCGCCTCTGATCATT
ACCAGACGCAACTCGCCGCTTCCATTTACAAAGGGGTATTGCGCTACTTTTCCAATGAACCCACGCTTCGTGAATAG

Upstream 100 bases:

>100_bases
GGGGAAAAAGCGGTACCGGCTAAAACTTCACTAAATGAGTTCTATTCCCGCGCCTTGTCCCATATAAGATAGAGAAGGGA
AATGCGCGGGAGGAGAAACG

Downstream 100 bases:

>100_bases
CGAGGGTTTTTTTGAGTAGAACAGCGGCAGCGAAACATGATCTTTACCAAAGGGAAATGGAAATCCTCATTCCCACTATA
AGTAAATGGGAAAGAAGGCT

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MKKKWKWLVAVAAITILGVLLFPSLFSDLTSTKPWNLPLSGRIIVLDPGHGGPDGGAVGGEVVEKEIALNVAKKLRDYLQ
QQGALVLMTRETDRDLASPSTRGYSRRKTEDLRERTTFINKSDADLFISIHLNAIPSPRWRGAQTFYYGSLIENERLAKF
IQAELRRNLENTHRVAKMIDTVYLLKHAKKPGALVEIGFLSNPDERELLASDHYQTQLAASIYKGVLRYFSNEPTLRE

Sequences:

>Translated_238_residues
MKKKWKWLVAVAAITILGVLLFPSLFSDLTSTKPWNLPLSGRIIVLDPGHGGPDGGAVGGEVVEKEIALNVAKKLRDYLQ
QQGALVLMTRETDRDLASPSTRGYSRRKTEDLRERTTFINKSDADLFISIHLNAIPSPRWRGAQTFYYGSLIENERLAKF
IQAELRRNLENTHRVAKMIDTVYLLKHAKKPGALVEIGFLSNPDERELLASDHYQTQLAASIYKGVLRYFSNEPTLRE
>Mature_238_residues
MKKKWKWLVAVAAITILGVLLFPSLFSDLTSTKPWNLPLSGRIIVLDPGHGGPDGGAVGGEVVEKEIALNVAKKLRDYLQ
QQGALVLMTRETDRDLASPSTRGYSRRKTEDLRERTTFINKSDADLFISIHLNAIPSPRWRGAQTFYYGSLIENERLAKF
IQAELRRNLENTHRVAKMIDTVYLLKHAKKPGALVEIGFLSNPDERELLASDHYQTQLAASIYKGVLRYFSNEPTLRE

Specific function: Unknown

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=234, Percent_Identity=26.4957264957265, Blast_Score=85, Evalue=3e-18,
Organism=Escherichia coli, GI87082163, Length=240, Percent_Identity=28.3333333333333, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR014234 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 26863; Mature: 26863

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKWKWLVAVAAITILGVLLFPSLFSDLTSTKPWNLPLSGRIIVLDPGHGGPDGGAVGG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCH
EVVEKEIALNVAKKLRDYLQQQGALVLMTRETDRDLASPSTRGYSRRKTEDLRERTTFIN
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHCCCCCCCCCHHHHHHHHHHHHHCC
KSDADLFISIHLNAIPSPRWRGAQTFYYGSLIENERLAKFIQAELRRNLENTHRVAKMID
CCCCEEEEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVYLLKHAKKPGALVEIGFLSNPDERELLASDHYQTQLAASIYKGVLRYFSNEPTLRE
HHHHHHHCCCCCCEEEEECCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKKKWKWLVAVAAITILGVLLFPSLFSDLTSTKPWNLPLSGRIIVLDPGHGGPDGGAVGG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCH
EVVEKEIALNVAKKLRDYLQQQGALVLMTRETDRDLASPSTRGYSRRKTEDLRERTTFIN
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHCCCCCCCCCHHHHHHHHHHHHHCC
KSDADLFISIHLNAIPSPRWRGAQTFYYGSLIENERLAKFIQAELRRNLENTHRVAKMID
CCCCEEEEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVYLLKHAKKPGALVEIGFLSNPDERELLASDHYQTQLAASIYKGVLRYFSNEPTLRE
HHHHHHHCCCCCCEEEEECCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7559346; 8969501; 9384377 [H]