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The map label for this gene is truA

Identifier: 138893815

GI number: 138893815

Start: 142918

End: 143679

Strand: Direct

Name: truA

Synonym: GTNG_0136

Alternate gene names: 138893815

Gene position: 142918-143679 (Clockwise)

Preceding gene: 138893814

Following gene: 138893816

Centisome position: 4.03

GC content: 55.25

Gene sequence:

>762_bases
ATGACACGACGAATCAAATGCGTGGTAGCGTATGATGGCACGTATTTTTCCGGCTATCAAGTTCAGCCGGGAAAACGTAC
GGTGCAAGGCGAGTTCGAGGGAGTGCTCAAGCGGATGCATAAAGGGGATGACGTGCGAATCACGGCTTCCGGGAGGACGG
ACGCCGGCGTTCACGCCTACGGGCAAGTCATTCACTTTGATACGCCGCTTGCTCTTTCCCCTGAGCAGTGGAAAAAGGCG
TTAAACGCCCAGCTTCCAGACGATATCGCTGTCCGCTCGGTAGACGAGGCAGACAGCACGTTTCACGCCCGTTTTTCCGC
CAAAGCGAAAGAGTACCGCTATAACGTATGGACGGCGGCCGAGCGCGACGTGTTCCGTCGCCATTATTGCGCATGGCATC
CGTACCCGCTCCGTGTAGACGCCATGAGCGAGGCACTCGGCTTGCTTAAGGGGACACACGATTTTACGAGCTTTTGTTCG
GCCAAAACGGTGATTGAAGACCGTGTGCGCACCATTTATCAGGCCGAAATGGAAGTGGATGGCCCAATGCTTCAGTTTCG
GTTTGTTGGAAGCGGCTTTTTATACAACATGGTGCGCATCATCGTTGGTACAGTGCTTGAGGTCGGCCAAGGGAAACGGC
CTCCTGACGATATTGAGGCGCTGCTTGCGGCGAAAGACCGTCGGCTTGCCGGTCCCACCGCGCCGGCTGAAGGGCTGTAT
TTATGGCGCGTGTATTATGATGGTGAAAGTTTCGGTCATTAG

Upstream 100 bases:

>100_bases
TCGTCAACTCGTCTGGAAGCCGTTGGACACGTCTTTTTTAGTCGCCACCGCGCTGCTGGCTGCATTGCTTTTTCTATTAC
GTTCATAGCGGAGGACTGCC

Downstream 100 bases:

>100_bases
CTGAGGGCTGCTCTTCTCTTCATATAAACTGGCCAAAAGCCCTTTAAGCATCGCAATACGAGTGTTACCTGTCGTTTCCA
ATTAGGAAGGAAGACAAACG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MTRRIKCVVAYDGTYFSGYQVQPGKRTVQGEFEGVLKRMHKGDDVRITASGRTDAGVHAYGQVIHFDTPLALSPEQWKKA
LNAQLPDDIAVRSVDEADSTFHARFSAKAKEYRYNVWTAAERDVFRRHYCAWHPYPLRVDAMSEALGLLKGTHDFTSFCS
AKTVIEDRVRTIYQAEMEVDGPMLQFRFVGSGFLYNMVRIIVGTVLEVGQGKRPPDDIEALLAAKDRRLAGPTAPAEGLY
LWRVYYDGESFGH

Sequences:

>Translated_253_residues
MTRRIKCVVAYDGTYFSGYQVQPGKRTVQGEFEGVLKRMHKGDDVRITASGRTDAGVHAYGQVIHFDTPLALSPEQWKKA
LNAQLPDDIAVRSVDEADSTFHARFSAKAKEYRYNVWTAAERDVFRRHYCAWHPYPLRVDAMSEALGLLKGTHDFTSFCS
AKTVIEDRVRTIYQAEMEVDGPMLQFRFVGSGFLYNMVRIIVGTVLEVGQGKRPPDDIEALLAAKDRRLAGPTAPAEGLY
LWRVYYDGESFGH
>Mature_252_residues
TRRIKCVVAYDGTYFSGYQVQPGKRTVQGEFEGVLKRMHKGDDVRITASGRTDAGVHAYGQVIHFDTPLALSPEQWKKAL
NAQLPDDIAVRSVDEADSTFHARFSAKAKEYRYNVWTAAERDVFRRHYCAWHPYPLRVDAMSEALGLLKGTHDFTSFCSA
KTVIEDRVRTIYQAEMEVDGPMLQFRFVGSGFLYNMVRIIVGTVLEVGQGKRPPDDIEALLAAKDRRLAGPTAPAEGLYL
WRVYYDGESFGH

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=265, Percent_Identity=32.4528301886792, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI199559785, Length=260, Percent_Identity=28.0769230769231, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI70166634, Length=275, Percent_Identity=26.5454545454545, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI70166599, Length=275, Percent_Identity=26.5454545454545, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI70166645, Length=278, Percent_Identity=26.6187050359712, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI1788657, Length=243, Percent_Identity=38.6831275720165, Blast_Score=157, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI71985046, Length=263, Percent_Identity=26.615969581749, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6321112, Length=242, Percent_Identity=28.099173553719, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281362146, Length=264, Percent_Identity=26.5151515151515, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648455, Length=264, Percent_Identity=26.5151515151515, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_GEOTN (A4IJL9)

Other databases:

- EMBL:   CP000557
- RefSeq:   YP_001124268.1
- ProteinModelPortal:   A4IJL9
- SMR:   A4IJL9
- STRING:   A4IJL9
- GeneID:   4964991
- GenomeReviews:   CP000557_GR
- KEGG:   gtn:GTNG_0136
- NMPDR:   fig|420246.5.peg.136
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- PhylomeDB:   A4IJL9
- ProtClustDB:   PRK00021
- BioCyc:   GTHE420246:GTNG_0136-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 28577; Mature: 28446

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: NA

Important sites: ACT_SITE 54-54

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRRIKCVVAYDGTYFSGYQVQPGKRTVQGEFEGVLKRMHKGDDVRITASGRTDAGVHAY
CCCEEEEEEEECCCEECCEEECCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHC
GQVIHFDTPLALSPEQWKKALNAQLPDDIAVRSVDEADSTFHARFSAKAKEYRYNVWTAA
CEEEEECCCCCCCHHHHHHHHCCCCCCHHHHCCCCCCCCCEEHHHHCCHHHEEEEEECHH
ERDVFRRHYCAWHPYPLRVDAMSEALGLLKGTHDFTSFCSAKTVIEDRVRTIYQAEMEVD
HHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GPMLQFRFVGSGFLYNMVRIIVGTVLEVGQGKRPPDDIEALLAAKDRRLAGPTAPAEGLY
CCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEE
LWRVYYDGESFGH
EEEEEECCCCCCC
>Mature Secondary Structure 
TRRIKCVVAYDGTYFSGYQVQPGKRTVQGEFEGVLKRMHKGDDVRITASGRTDAGVHAY
CCEEEEEEEECCCEECCEEECCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHC
GQVIHFDTPLALSPEQWKKALNAQLPDDIAVRSVDEADSTFHARFSAKAKEYRYNVWTAA
CEEEEECCCCCCCHHHHHHHHCCCCCCHHHHCCCCCCCCCEEHHHHCCHHHEEEEEECHH
ERDVFRRHYCAWHPYPLRVDAMSEALGLLKGTHDFTSFCSAKTVIEDRVRTIYQAEMEVD
HHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GPMLQFRFVGSGFLYNMVRIIVGTVLEVGQGKRPPDDIEALLAAKDRRLAGPTAPAEGLY
CCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEE
LWRVYYDGESFGH
EEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA