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The map label for this gene is ppa

Identifier: 13508267

GI number: 13508267

Start: 649839

End: 650393

Strand: Direct

Name: ppa

Synonym: MPN528

Alternate gene names: 13508267

Gene position: 649839-650393 (Clockwise)

Preceding gene: 13508265

Following gene: 13508428

Centisome position: 79.6

GC content: 37.12

Gene sequence:

>555_bases
ATGGACAAATTTTTAATCGATGTTACTGTAGAAATTCCTAAGAGCAGTAAGATCAAGTATGAATACGACCGCAAGACTAG
TCAAATCCGTGTCGACAGGATTCTCTTTGGTAGTGAATCTTATCCACAAAACTATGGTTTTATCGCTAACACCTTAGACT
GGGATGGTGATGAATTGGACTGTTTTATCTTCGCTGACCAAGCCTTTCTACCAGGTGTAGTAGTACCTACTAGAATTGTA
GGAGCTTTAGAAATGGTAGATGACGGTGAATTGGATACCAAACTTTTAGGGGTCATTGATTGTGATCCGCGTTACAAAGA
GATTAACTCCGTCAATGATTTACCAAAACACCGCGTTGATGAAATTATTGGTTTTCTCAAAACTTACAAATTACTACAAA
AGAAAGAAGTAATTATTAAGGGAGTGCAAAGTTTGGAGTGGGCTAAGAAAGAATACCAAGTGTGTGTTGATTTAATGAAG
CAATATGGTAAGTTGCCTAAGGATGAGTTTATCGCTAAAATGCAAAAACTCCATCCTGAACACTACCAAAAATAG

Upstream 100 bases:

>100_bases
GGTGTTGTTCTTTTTTTGGCCAAAAAGCTATTCTTGCACCATTCATTATTTAGTAAAGCCTACTAGCTAGAAAAGTAGTT
AAATAATAATATTTATTAAT

Downstream 100 bases:

>100_bases
TTCAAGTTATGATTAGCGCTAACCGTGGCATGTTTTTGGAAACAATTGTTAACCAAACAATTGTACGACTAGGAGATCAT
CCAGATATTTGACTCGAAAA

Product: inorganic pyrophosphatase

Products: NA

Alternate protein names: Pyrophosphate phospho-hydrolase; PPase

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MDKFLIDVTVEIPKSSKIKYEYDRKTSQIRVDRILFGSESYPQNYGFIANTLDWDGDELDCFIFADQAFLPGVVVPTRIV
GALEMVDDGELDTKLLGVIDCDPRYKEINSVNDLPKHRVDEIIGFLKTYKLLQKKEVIIKGVQSLEWAKKEYQVCVDLMK
QYGKLPKDEFIAKMQKLHPEHYQK

Sequences:

>Translated_184_residues
MDKFLIDVTVEIPKSSKIKYEYDRKTSQIRVDRILFGSESYPQNYGFIANTLDWDGDELDCFIFADQAFLPGVVVPTRIV
GALEMVDDGELDTKLLGVIDCDPRYKEINSVNDLPKHRVDEIIGFLKTYKLLQKKEVIIKGVQSLEWAKKEYQVCVDLMK
QYGKLPKDEFIAKMQKLHPEHYQK
>Mature_184_residues
MDKFLIDVTVEIPKSSKIKYEYDRKTSQIRVDRILFGSESYPQNYGFIANTLDWDGDELDCFIFADQAFLPGVVVPTRIV
GALEMVDDGELDTKLLGVIDCDPRYKEINSVNDLPKHRVDEIIGFLKTYKLLQKKEVIIKGVQSLEWAKKEYQVCVDLMK
QYGKLPKDEFIAKMQKLHPEHYQK

Specific function: Unknown

COG id: COG0221

COG function: function code C; Inorganic pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PPase family

Homologues:

Organism=Escherichia coli, GI1790673, Length=150, Percent_Identity=38.6666666666667, Blast_Score=91, Evalue=4e-20,

Paralogues:

None

Copy number: 5480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): IPYR_MYCPN (P75250)

Other databases:

- EMBL:   U00089
- PIR:   S73640
- RefSeq:   NP_110216.1
- ProteinModelPortal:   P75250
- GeneID:   876725
- GenomeReviews:   U00089_GR
- KEGG:   mpn:MPN528
- HOGENOM:   HBG529150
- OMA:   MIDDGEI
- ProtClustDB:   PRK02230
- BioCyc:   MetaCyc:MONOMER-644
- BioCyc:   MPNE272634:MPN528-MONOMER
- BRENDA:   3.6.1.1
- GO:   GO:0005737
- HAMAP:   MF_00209
- InterPro:   IPR008162
- Gene3D:   G3DSA:3.90.80.10
- PANTHER:   PTHR10286

Pfam domain/function: PF00719 Pyrophosphatase; SSF50324 Pyrophosphatase

EC number: =3.6.1.1

Molecular weight: Translated: 21369; Mature: 21369

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00387 PPASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKFLIDVTVEIPKSSKIKYEYDRKTSQIRVDRILFGSESYPQNYGFIANTLDWDGDELD
CCCEEEEEEEECCCCCCEEEEECCCCHHEEEEHEEECCCCCCCCCCCEEECCCCCCCCEE
CFIFADQAFLPGVVVPTRIVGALEMVDDGELDTKLLGVIDCDPRYKEINSVNDLPKHRVD
EEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHCHHCCHHHHHH
EIIGFLKTYKLLQKKEVIIKGVQSLEWAKKEYQVCVDLMKQYGKLPKDEFIAKMQKLHPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHH
HYQK
HCCC
>Mature Secondary Structure
MDKFLIDVTVEIPKSSKIKYEYDRKTSQIRVDRILFGSESYPQNYGFIANTLDWDGDELD
CCCEEEEEEEECCCCCCEEEEECCCCHHEEEEHEEECCCCCCCCCCCEEECCCCCCCCEE
CFIFADQAFLPGVVVPTRIVGALEMVDDGELDTKLLGVIDCDPRYKEINSVNDLPKHRVD
EEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHCHHCCHHHHHH
EIIGFLKTYKLLQKKEVIIKGVQSLEWAKKEYQVCVDLMKQYGKLPKDEFIAKMQKLHPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHH
HYQK
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633