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The map label for this gene is artJ [H]

Identifier: 13474707

GI number: 13474707

Start: 4544702

End: 4545475

Strand: Direct

Name: artJ [H]

Synonym: mlr5654

Alternate gene names: 13474707

Gene position: 4544702-4545475 (Clockwise)

Preceding gene: 13474706

Following gene: 13474712

Centisome position: 64.59

GC content: 60.47

Gene sequence:

>774_bases
ATGCAGATCTCGAAATGGATCATGTCGGCGGCAGGCGCTGCCATGTTGGCGATGGCCGCCGGCTCTCTGACGGCGCAGGC
TGCCGAGAAATTGAAGATCGGCACCGAGGGCGCCTATCCGCCGTTCAACACCATCACCTCGGACGGCAAGGTTGTCGGCT
TCGATATCGACATCGCCAATGCGCTGTGTGCTCAGATGAAGGTCGAGTGCGAGATCGTCACCCAGGACTGGGATGGCATC
ATCCCCGCCCTGCAGGCCAAGAAGTTCGACGCCATCATCGCCTCGATGAGCATCACGGAAGAGCGCAAGAAGCAGGTCGC
CTTCACCAACAAATACTACACAACGCCGCTCGCTCTGGTGGCGCCGAAGGACAGCGATCTGACCTCGGCAGACCCGGCGG
CCCTTGCTGGCAAGACGGTCGGCGCCCAGGCTTCGACCACACAGGCCGACTATGCCCAGAACACCTATGGCAAGGCGGGT
GCCGAGGCAAAGCTCTACCCAACCCAGGAAGAGGCGATCACCGACCTGACCAATGGCCGGCTTGATGCCGTGATCTCGGA
CAAGTTCGTGCTGGTGGACTGGCTGAAGAAGGCAAGCGACGGTTGCTGCAAGCTGGTCGGCGACGTCAAGGGCACGGAAA
CACAAGCCGGCATCGCCGTGCGCCTCGACGACACCGCCCTGCGCGACAAGCTCAATGCGGCCATCGATGCGATCGTCGCA
GATGGCACCTACAAGAAGATCCAGGCCAAGTACTTCGATTTTGATATTTATTGA

Upstream 100 bases:

>100_bases
ACCCCGATTGCTGCCAGGCAAGATACTTGCCTGCGCTGGCACATCATGCTTGGCTGGCTTGTCGGCCGTCGAGATGGCGG
CGCTCAAGGGGAACAAGAAA

Downstream 100 bases:

>100_bases
GGTTGAAGAGCGCCTCTTCCTTCTCCCACAGGGGAGAAGGGGAAGCTGGCGCTACAGCGCCTTCACCGCCACCTTCACCG
GCTTTTCGATCTCGAGCGGG

Product: ABC transporter, periplasmic amino acid-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MQISKWIMSAAGAAMLAMAAGSLTAQAAEKLKIGTEGAYPPFNTITSDGKVVGFDIDIANALCAQMKVECEIVTQDWDGI
IPALQAKKFDAIIASMSITEERKKQVAFTNKYYTTPLALVAPKDSDLTSADPAALAGKTVGAQASTTQADYAQNTYGKAG
AEAKLYPTQEEAITDLTNGRLDAVISDKFVLVDWLKKASDGCCKLVGDVKGTETQAGIAVRLDDTALRDKLNAAIDAIVA
DGTYKKIQAKYFDFDIY

Sequences:

>Translated_257_residues
MQISKWIMSAAGAAMLAMAAGSLTAQAAEKLKIGTEGAYPPFNTITSDGKVVGFDIDIANALCAQMKVECEIVTQDWDGI
IPALQAKKFDAIIASMSITEERKKQVAFTNKYYTTPLALVAPKDSDLTSADPAALAGKTVGAQASTTQADYAQNTYGKAG
AEAKLYPTQEEAITDLTNGRLDAVISDKFVLVDWLKKASDGCCKLVGDVKGTETQAGIAVRLDDTALRDKLNAAIDAIVA
DGTYKKIQAKYFDFDIY
>Mature_257_residues
MQISKWIMSAAGAAMLAMAAGSLTAQAAEKLKIGTEGAYPPFNTITSDGKVVGFDIDIANALCAQMKVECEIVTQDWDGI
IPALQAKKFDAIIASMSITEERKKQVAFTNKYYTTPLALVAPKDSDLTSADPAALAGKTVGAQASTTQADYAQNTYGKAG
AEAKLYPTQEEAITDLTNGRLDAVISDKFVLVDWLKKASDGCCKLVGDVKGTETQAGIAVRLDDTALRDKLNAAIDAIVA
DGTYKKIQAKYFDFDIY

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=237, Percent_Identity=41.7721518987342, Blast_Score=182, Evalue=1e-47,
Organism=Escherichia coli, GI1788648, Length=234, Percent_Identity=39.3162393162393, Blast_Score=167, Evalue=7e-43,
Organism=Escherichia coli, GI1788649, Length=260, Percent_Identity=36.9230769230769, Blast_Score=161, Evalue=4e-41,
Organism=Escherichia coli, GI1787088, Length=242, Percent_Identity=39.6694214876033, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1787031, Length=241, Percent_Identity=35.2697095435685, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1788228, Length=248, Percent_Identity=28.6290322580645, Blast_Score=118, Evalue=5e-28,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27370; Mature: 27370

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQISKWIMSAAGAAMLAMAAGSLTAQAAEKLKIGTEGAYPPFNTITSDGKVVGFDIDIAN
CCHHHHHHHHCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEHHHH
ALCAQMKVECEIVTQDWDGIIPALQAKKFDAIIASMSITEERKKQVAFTNKYYTTPLALV
HHHHHEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEECCEEEE
APKDSDLTSADPAALAGKTVGAQASTTQADYAQNTYGKAGAEAKLYPTQEEAITDLTNGR
ECCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHCCCCCCCCCCEECCCHHHHHHHHCCCC
LDAVISDKFVLVDWLKKASDGCCKLVGDVKGTETQAGIAVRLDDTALRDKLNAAIDAIVA
EEEEECCCCHHHHHHHHCCCCCEEECCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCC
DGTYKKIQAKYFDFDIY
CCCHHEEEEEEEEEECC
>Mature Secondary Structure
MQISKWIMSAAGAAMLAMAAGSLTAQAAEKLKIGTEGAYPPFNTITSDGKVVGFDIDIAN
CCHHHHHHHHCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEHHHH
ALCAQMKVECEIVTQDWDGIIPALQAKKFDAIIASMSITEERKKQVAFTNKYYTTPLALV
HHHHHEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEECCEEEE
APKDSDLTSADPAALAGKTVGAQASTTQADYAQNTYGKAGAEAKLYPTQEEAITDLTNGR
ECCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHCCCCCCCCCCEECCCHHHHHHHHCCCC
LDAVISDKFVLVDWLKKASDGCCKLVGDVKGTETQAGIAVRLDDTALRDKLNAAIDAIVA
EEEEECCCCHHHHHHHHCCCCCEEECCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHCC
DGTYKKIQAKYFDFDIY
CCCHHEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]