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The map label for this gene is basR [H]

Identifier: 13474480

GI number: 13474480

Start: 4293427

End: 4294101

Strand: Reverse

Name: basR [H]

Synonym: mll5373

Alternate gene names: 13474480

Gene position: 4294101-4293427 (Counterclockwise)

Preceding gene: 13474484

Following gene: 13474479

Centisome position: 61.03

GC content: 67.7

Gene sequence:

>675_bases
ATGCGTATCTTGCTGGTCGAAGACGAGCCCGAGATGGTCTCGGCGCTGCGTGCCGCGCTGAAGCGCCACGACATGGTGGT
CGATCACGCCGGCACATTGCTTGAGGCGGAAGGCTTCGTCGCCGTCGACAGCTACGACGCCATCCTGCTCGACCGCCAGT
TGCCCGACGGCGACGGGCTGTCGCTGGTGCCGAGATTGCGCTCGGCGGCGAACACCACGCCGGTGCTGGTGCTGACGGCC
AAGGGCGACACGTCCGACAAGGTCGATGGGCTGGACATGGGCGCGGACGACTATCTGGCAAAGCCGTTCGCCTTCGAGGA
ATTGCTGGCCCGCTTGCGCGCATTGCTCAGGCGGCCGGCGCCGCTGCAGTCGCAATTCATCCGCGCCGGCCATCTGGTGC
TCGATGTCGGACATCGCGAGGCGGCGATCGGCGGCGAGCCGCTCAGCCTGCCGCGCCGCGAACTGCTGGTGCTGGAAGCA
CTGATGCGGCGCACCGGCCGCATGGTGCAGCGCGAGGCGCTGATGGAAGCGGTGTTCGGCCTCGACGACGAGATCCAATC
CAACGCGCTCGACACCCATGTCTCGCGCCTGCGCCGCAAGCTCGCCGACGCCGATGCCGGCGTGACGATCAACGGCATTC
GCGGCGTCGGCTATCTGCTGCGCGAAACGACGTGA

Upstream 100 bases:

>100_bases
AGAAAGGACTTGCCGGCCACGGGCCATTCGGCTTTGCCATGTCTGCGCAATGTTGGCGCGGTAGCGCCCGATGCGGTGTG
CTCGTTGCCGGGGAGTATGA

Downstream 100 bases:

>100_bases
GCCTCAATCGATGAGCCTCAAGCGCCCGCGTTCGCTCAAATGGAGCCTGGTGCTGCGCATCGCCTTACTGCAATGCGCGA
TGCTGACGCTGATCATCGTC

Product: two-component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MRILLVEDEPEMVSALRAALKRHDMVVDHAGTLLEAEGFVAVDSYDAILLDRQLPDGDGLSLVPRLRSAANTTPVLVLTA
KGDTSDKVDGLDMGADDYLAKPFAFEELLARLRALLRRPAPLQSQFIRAGHLVLDVGHREAAIGGEPLSLPRRELLVLEA
LMRRTGRMVQREALMEAVFGLDDEIQSNALDTHVSRLRRKLADADAGVTINGIRGVGYLLRETT

Sequences:

>Translated_224_residues
MRILLVEDEPEMVSALRAALKRHDMVVDHAGTLLEAEGFVAVDSYDAILLDRQLPDGDGLSLVPRLRSAANTTPVLVLTA
KGDTSDKVDGLDMGADDYLAKPFAFEELLARLRALLRRPAPLQSQFIRAGHLVLDVGHREAAIGGEPLSLPRRELLVLEA
LMRRTGRMVQREALMEAVFGLDDEIQSNALDTHVSRLRRKLADADAGVTINGIRGVGYLLRETT
>Mature_224_residues
MRILLVEDEPEMVSALRAALKRHDMVVDHAGTLLEAEGFVAVDSYDAILLDRQLPDGDGLSLVPRLRSAANTTPVLVLTA
KGDTSDKVDGLDMGADDYLAKPFAFEELLARLRALLRRPAPLQSQFIRAGHLVLDVGHREAAIGGEPLSLPRRELLVLEA
LMRRTGRMVQREALMEAVFGLDDEIQSNALDTHVSRLRRKLADADAGVTINGIRGVGYLLRETT

Specific function: Member of the two-component regulatory system BasS/BasR. BasR induces the transcription of the ugd, ais, arnBCADTEF and eptA-basRS loci, all involved in resistance to polymyxin [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790552, Length=220, Percent_Identity=40.9090909090909, Blast_Score=157, Evalue=4e-40,
Organism=Escherichia coli, GI1789402, Length=222, Percent_Identity=39.6396396396396, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1786784, Length=222, Percent_Identity=39.6396396396396, Blast_Score=146, Evalue=9e-37,
Organism=Escherichia coli, GI1787375, Length=220, Percent_Identity=36.8181818181818, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI87082012, Length=218, Percent_Identity=35.3211009174312, Blast_Score=121, Evalue=3e-29,
Organism=Escherichia coli, GI1789809, Length=227, Percent_Identity=34.8017621145374, Blast_Score=121, Evalue=3e-29,
Organism=Escherichia coli, GI1786599, Length=220, Percent_Identity=33.6363636363636, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1790860, Length=226, Percent_Identity=35.3982300884956, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1788394, Length=225, Percent_Identity=35.1111111111111, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI2367329, Length=241, Percent_Identity=36.0995850622407, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1786911, Length=205, Percent_Identity=37.0731707317073, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1787229, Length=226, Percent_Identity=32.7433628318584, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1790863, Length=227, Percent_Identity=29.0748898678414, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI145693140, Length=231, Percent_Identity=29.004329004329, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 24553; Mature: 24553

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILLVEDEPEMVSALRAALKRHDMVVDHAGTLLEAEGFVAVDSYDAILLDRQLPDGDGL
CEEEEECCCHHHHHHHHHHHHHHCCHHHCCCCEEECCCEEEECCCCEEEEECCCCCCCCC
SLVPRLRSAANTTPVLVLTAKGDTSDKVDGLDMGADDYLAKPFAFEELLARLRALLRRPA
HHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHCCCC
PLQSQFIRAGHLVLDVGHREAAIGGEPLSLPRRELLVLEALMRRTGRMVQREALMEAVFG
CHHHHHHHHCCEEEECCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LDDEIQSNALDTHVSRLRRKLADADAGVTINGIRGVGYLLRETT
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHCCC
>Mature Secondary Structure
MRILLVEDEPEMVSALRAALKRHDMVVDHAGTLLEAEGFVAVDSYDAILLDRQLPDGDGL
CEEEEECCCHHHHHHHHHHHHHHCCHHHCCCCEEECCCEEEECCCCEEEEECCCCCCCCC
SLVPRLRSAANTTPVLVLTAKGDTSDKVDGLDMGADDYLAKPFAFEELLARLRALLRRPA
HHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHCCCC
PLQSQFIRAGHLVLDVGHREAAIGGEPLSLPRRELLVLEALMRRTGRMVQREALMEAVFG
CHHHHHHHHCCEEEECCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LDDEIQSNALDTHVSRLRRKLADADAGVTINGIRGVGYLLRETT
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8282725; 7610040; 9278503 [H]