Click here to switch to the map view.

The map label for this gene is dhaK [H]

Identifier: 13474411

GI number: 13474411

Start: 4218374

End: 4219360

Strand: Reverse

Name: dhaK [H]

Synonym: mll5289

Alternate gene names: 13474411

Gene position: 4219360-4218374 (Counterclockwise)

Preceding gene: 13474412

Following gene: 13474409

Centisome position: 59.97

GC content: 65.86

Gene sequence:

>987_bases
ATGAAGAAATTTATGAACGCGGTGGACACGGTGCTGACCGAGAGCCTCGACGGTTTCGTGGCTGCCCATGCCGATATCCT
CGCGCTTGGCGAAGAACACAAATTCGTCCGCCGCAGGGCGTTGCGGCCGGGCAAGGTGGCGCTGATCTCCGGCGGCGGCT
CGGGCCACGAGCCGCTGCATGCCGGCTTTGTCGGCCATGGCATGCTGGACGCCGCCTGCCCCGGCCAGGTGTTCACCTCG
CCGACGCCGGACCAGATGCTGGCGGCGACGCAGGCCGTCGACAGCGGCGCCGGCTGCCTGTTCATCGTCAAGAATTACGA
GGGCGATGTGATGAATTTCGACATGGCGGCCGAAATGTCTGACGGCGTTCTACGGGTGGTGACCAATGACGATGTCGCCG
TCGAGAATTCGTCCTATACGACCGGCCGTCGCGGCGTCGCCGGCACGCTGGTGGTGGAAAAGATCGTCGGCGCGGCGGCC
GAGCATGGCCTGGCGCTGCCGGTGCTGAAGGCGCTGGGCGAGCGCGTCAACGCCGCCACCCGCTCGATGGGCGTGGCGCT
GACCAGCGGCACCGTGCCGGCGGCAGGCAAGCCGACCTTCGATATCGGCGACGGCGAGATGGAATTCGGCGTCGGCATCC
ATGGCGAACCCGGCCGCCGGCGCGACACGTTGAAGAGCGCGGACGCGATCGCCGAGGAAATCTGCGCGTCGATCCTGGGC
GATCTCGGCGACAAGGCGAAAGGCCCCGCGCTGCTGTTCGTCAACGGCTTCGGCGGCACGCCGCTGATGGAGCTCTATCT
GATGTACAACAGCGCGCGAAGAATTCTCGAGAAGCGTGGCGTGACGATAGTCCGTTCGCTTGTCGGCAGCTATGTCACCT
CGCTCGACATGGCCGGCTGCTCGATCACGCTCACCATGCTCGATGACGAGATGACCGGCTGGTGGGATGCGCCGGTCCAT
ACGGCGGCATTGCGCTGGGGCATGTAG

Upstream 100 bases:

>100_bases
GCGCTCGACCGGGCGATCAACGAATTCATCCAGAAGATCACCAGCATCGCCGGCAAGGGCGGCAGCTGACCATTCTTGCC
GGGCTGTTTGGGAGGACGCC

Downstream 100 bases:

>100_bases
AAATTCGAGTGCGCGGCGATTTTCCCTGAAAGGAGCCATAGCGGTTCCGTTGGGAATTGTGCATGGCGATCTATGCGCCA
TCACCTGCTCTCAGCGGAAT

Product: dihydroxyacetone kinase subunit DhaK

Products: ADP; glycerone phosphate

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MKKFMNAVDTVLTESLDGFVAAHADILALGEEHKFVRRRALRPGKVALISGGGSGHEPLHAGFVGHGMLDAACPGQVFTS
PTPDQMLAATQAVDSGAGCLFIVKNYEGDVMNFDMAAEMSDGVLRVVTNDDVAVENSSYTTGRRGVAGTLVVEKIVGAAA
EHGLALPVLKALGERVNAATRSMGVALTSGTVPAAGKPTFDIGDGEMEFGVGIHGEPGRRRDTLKSADAIAEEICASILG
DLGDKAKGPALLFVNGFGGTPLMELYLMYNSARRILEKRGVTIVRSLVGSYVTSLDMAGCSITLTMLDDEMTGWWDAPVH
TAALRWGM

Sequences:

>Translated_328_residues
MKKFMNAVDTVLTESLDGFVAAHADILALGEEHKFVRRRALRPGKVALISGGGSGHEPLHAGFVGHGMLDAACPGQVFTS
PTPDQMLAATQAVDSGAGCLFIVKNYEGDVMNFDMAAEMSDGVLRVVTNDDVAVENSSYTTGRRGVAGTLVVEKIVGAAA
EHGLALPVLKALGERVNAATRSMGVALTSGTVPAAGKPTFDIGDGEMEFGVGIHGEPGRRRDTLKSADAIAEEICASILG
DLGDKAKGPALLFVNGFGGTPLMELYLMYNSARRILEKRGVTIVRSLVGSYVTSLDMAGCSITLTMLDDEMTGWWDAPVH
TAALRWGM
>Mature_328_residues
MKKFMNAVDTVLTESLDGFVAAHADILALGEEHKFVRRRALRPGKVALISGGGSGHEPLHAGFVGHGMLDAACPGQVFTS
PTPDQMLAATQAVDSGAGCLFIVKNYEGDVMNFDMAAEMSDGVLRVVTNDDVAVENSSYTTGRRGVAGTLVVEKIVGAAA
EHGLALPVLKALGERVNAATRSMGVALTSGTVPAAGKPTFDIGDGEMEFGVGIHGEPGRRRDTLKSADAIAEEICASILG
DLGDKAKGPALLFVNGFGGTPLMELYLMYNSARRILEKRGVTIVRSLVGSYVTSLDMAGCSITLTMLDDEMTGWWDAPVH
TAALRWGM

Specific function: Dihydroxyacetone binding subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone. Binds covalently dihydroxyacetone in hemiaminal linkage. Acts also as a corepressor of dhaR by binding to its sensor domain, in the

COG id: COG2376

COG function: function code G; Dihydroxyacetone kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DhaK domain [H]

Homologues:

Organism=Homo sapiens, GI20149621, Length=349, Percent_Identity=38.9684813753582, Blast_Score=201, Evalue=6e-52,
Organism=Escherichia coli, GI87081857, Length=356, Percent_Identity=47.752808988764, Blast_Score=312, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI17565018, Length=326, Percent_Identity=36.5030674846626, Blast_Score=195, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6321055, Length=301, Percent_Identity=36.5448504983389, Blast_Score=168, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6323570, Length=312, Percent_Identity=36.5384615384615, Blast_Score=153, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004006
- InterPro:   IPR012736 [H]

Pfam domain/function: PF02733 Dak1 [H]

EC number: 2.7.1.29

Molecular weight: Translated: 34441; Mature: 34441

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFMNAVDTVLTESLDGFVAAHADILALGEEHKFVRRRALRPGKVALISGGGSGHEPLH
CHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHCCCCCCEEEEECCCCCCCCCC
AGFVGHGMLDAACPGQVFTSPTPDQMLAATQAVDSGAGCLFIVKNYEGDVMNFDMAAEMS
CCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEHHHCCC
DGVLRVVTNDDVAVENSSYTTGRRGVAGTLVVEKIVGAAAEHGLALPVLKALGERVNAAT
CCEEEEEECCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RSMGVALTSGTVPAAGKPTFDIGDGEMEFGVGIHGEPGRRRDTLKSADAIAEEICASILG
HHCCEEEECCCCCCCCCCCEECCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DLGDKAKGPALLFVNGFGGTPLMELYLMYNSARRILEKRGVTIVRSLVGSYVTSLDMAGC
HCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
SITLTMLDDEMTGWWDAPVHTAALRWGM
EEEEEEECCCCCCCCCCCHHHHHHHCCC
>Mature Secondary Structure
MKKFMNAVDTVLTESLDGFVAAHADILALGEEHKFVRRRALRPGKVALISGGGSGHEPLH
CHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHCCCCCCEEEEECCCCCCCCCC
AGFVGHGMLDAACPGQVFTSPTPDQMLAATQAVDSGAGCLFIVKNYEGDVMNFDMAAEMS
CCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEHHHCCC
DGVLRVVTNDDVAVENSSYTTGRRGVAGTLVVEKIVGAAAEHGLALPVLKALGERVNAAT
CCEEEEEECCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RSMGVALTSGTVPAAGKPTFDIGDGEMEFGVGIHGEPGRRRDTLKSADAIAEEICASILG
HHCCEEEECCCCCCCCCCCEECCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
DLGDKAKGPALLFVNGFGGTPLMELYLMYNSARRILEKRGVTIVRSLVGSYVTSLDMAGC
HCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
SITLTMLDDEMTGWWDAPVHTAALRWGM
EEEEEEECCCCCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; glycerone

Specific reaction: ATP + glycerone = ADP + glycerone phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]