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The map label for this gene is ntaA [H]

Identifier: 13474284

GI number: 13474284

Start: 4075691

End: 4076938

Strand: Reverse

Name: ntaA [H]

Synonym: mll5137

Alternate gene names: 13474284

Gene position: 4076938-4075691 (Counterclockwise)

Preceding gene: 13474285

Following gene: 13474279

Centisome position: 57.94

GC content: 66.59

Gene sequence:

>1248_bases
ATGACCGCGCAGATGAAGCTCGGCGCCTTCCTGTGGGCGACTGGACACCATATCGCTGCCTGGCGGCACCCCAAAGCACA
TGTGACGGCCGGCATCGACATCGACCACTACATCCAGCTGGCGCGCACGGCGGAAGCGGCGAAGTTCGACATGATCTTCT
GCGAGGACGCCGCCGGCCTGCGCGAAGCCAATGTGGGCATCGCCAGCCAGACGTCGCGCTCGATCGGCTTCGAGCCGATC
AGCCTGCTGTCGGCACTGGCCGTGCAGACCAGCCGCATCGGCCTCGTGTCGACGGCATCGACAAGCTACAACGAGCCTTA
CGGGCTGGCGCGGACGTTCCTGTCGCTCGACCATCTGAGCGGCGGCCGGGCCGGCTGGAACCTGGTCACCTCGGCCAGCC
CCATCGAAGCCGCCAATTTCGGCTCGACGGGCCTCAAGCCGCATGCCGACCGCTACGAGCGGGCGCGCGAATTCGCCGGG
GTTGTCACCGGCCTATGGCGTGGCAAGGCGTCCGGCCATGACGGCCAGAGCTTTTCGGTGCGCGATCCGCTCGACCTGCC
GCGTTCGCCACAGGGCGCACCAGTGATGGTCCAGGCCGGCGCCTCGGATGTCGGCCGCGACCTTGCCGCCCGCACCGCCG
ATGTGGTGTTCACGGCGGCGCAGACCTTCGAGGAAGCCAAGGCCTTTTATGACGATCTCAAGGGGCGCATGGCAGCCTAT
GGGCGTGAGCCTGACGACATCAAGATCATGCCCGGCGTCTCGCCCGTTGTGGCGGAAACCGAGGCCGAAGCCCGGGAGAA
ACACGAGGAATTGCAGGTGCTGATCCCCGACGATGTCGGCGTGGCGCTGCTGTCCAGCTATCTCAGTATCTCCGATCTCT
GGCGCTATCCGATCGACGGGCCGCTGCCCGAACTGCCGGAGAGCGAGGGAATGAAAAGCCGGCAGGCGCTGGTCGTCGAG
CAATCGCGCCGCGACGGCCTTTCCATCCGCCAACTGGCTCGCCATTTCGCCGGCGCGCGCGGCCATTGGCGCATCGTCGG
CACGGCGGCGCAGGTCGCCGACGAATTGCAGGCTCGATTCGAAGGCGGTGCGGCCGACGGCTTCAACGTCATGCCGTCTT
ATTTCCCGGGAGAACTCGATGCCTTTGCCACGCTGGTGGTGCCGGAGCTGCAGCGGCGCGGCCTGTTCCGTCGGGACTAT
GAGGGTCGCACCTTGCGCGACCATCTGGGCTTGAAACGGCCGGTTTAG

Upstream 100 bases:

>100_bases
ATCATATTCTGCCGCCGTTGCGGCGGCGTGGCCTTGTTCGAGAGACCTATCTCGGCACAACGCTGCGCTCGCATCTTGGA
CTGGCCGGCGGAGGTGACCG

Downstream 100 bases:

>100_bases
CCGTAATGCTAGCTTAGCCGGCCATCACCCTGTCATGCGGATGGTCGATATTTGGTGCCGCGTTGTGAGTGACCAGCGCC
GTCATGATCTCCGCGGCTGC

Product: nitrilotriacetate monooxygenase component A

Products: an aldehyde; SO3; H2O; FMN [C]

Alternate protein names: NTA monooxygenase component A; NTA-MO A [H]

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MTAQMKLGAFLWATGHHIAAWRHPKAHVTAGIDIDHYIQLARTAEAAKFDMIFCEDAAGLREANVGIASQTSRSIGFEPI
SLLSALAVQTSRIGLVSTASTSYNEPYGLARTFLSLDHLSGGRAGWNLVTSASPIEAANFGSTGLKPHADRYERAREFAG
VVTGLWRGKASGHDGQSFSVRDPLDLPRSPQGAPVMVQAGASDVGRDLAARTADVVFTAAQTFEEAKAFYDDLKGRMAAY
GREPDDIKIMPGVSPVVAETEAEAREKHEELQVLIPDDVGVALLSSYLSISDLWRYPIDGPLPELPESEGMKSRQALVVE
QSRRDGLSIRQLARHFAGARGHWRIVGTAAQVADELQARFEGGAADGFNVMPSYFPGELDAFATLVVPELQRRGLFRRDY
EGRTLRDHLGLKRPV

Sequences:

>Translated_415_residues
MTAQMKLGAFLWATGHHIAAWRHPKAHVTAGIDIDHYIQLARTAEAAKFDMIFCEDAAGLREANVGIASQTSRSIGFEPI
SLLSALAVQTSRIGLVSTASTSYNEPYGLARTFLSLDHLSGGRAGWNLVTSASPIEAANFGSTGLKPHADRYERAREFAG
VVTGLWRGKASGHDGQSFSVRDPLDLPRSPQGAPVMVQAGASDVGRDLAARTADVVFTAAQTFEEAKAFYDDLKGRMAAY
GREPDDIKIMPGVSPVVAETEAEAREKHEELQVLIPDDVGVALLSSYLSISDLWRYPIDGPLPELPESEGMKSRQALVVE
QSRRDGLSIRQLARHFAGARGHWRIVGTAAQVADELQARFEGGAADGFNVMPSYFPGELDAFATLVVPELQRRGLFRRDY
EGRTLRDHLGLKRPV
>Mature_414_residues
TAQMKLGAFLWATGHHIAAWRHPKAHVTAGIDIDHYIQLARTAEAAKFDMIFCEDAAGLREANVGIASQTSRSIGFEPIS
LLSALAVQTSRIGLVSTASTSYNEPYGLARTFLSLDHLSGGRAGWNLVTSASPIEAANFGSTGLKPHADRYERAREFAGV
VTGLWRGKASGHDGQSFSVRDPLDLPRSPQGAPVMVQAGASDVGRDLAARTADVVFTAAQTFEEAKAFYDDLKGRMAAYG
REPDDIKIMPGVSPVVAETEAEAREKHEELQVLIPDDVGVALLSSYLSISDLWRYPIDGPLPELPESEGMKSRQALVVEQ
SRRDGLSIRQLARHFAGARGHWRIVGTAAQVADELQARFEGGAADGFNVMPSYFPGELDAFATLVVPELQRRGLFRRDYE
GRTLRDHLGLKRPV

Specific function: Hydroxylation of nitrilotriacetate [H]

COG id: COG2141

COG function: function code C; Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ntaA/snaA/soxA(dszA) monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1787166, Length=183, Percent_Identity=32.2404371584699, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1787247, Length=158, Percent_Identity=32.9113924050633, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011251
- InterPro:   IPR016048
- InterPro:   IPR016215 [H]

Pfam domain/function: PF00296 Bac_luciferase [H]

EC number: 1.14.14.5 [C]

Molecular weight: Translated: 45093; Mature: 44962

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQMKLGAFLWATGHHIAAWRHPKAHVTAGIDIDHYIQLARTAEAAKFDMIFCEDAAGL
CCCCEECCEEEEECCCEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHEEEEEECCCCCC
REANVGIASQTSRSIGFEPISLLSALAVQTSRIGLVSTASTSYNEPYGLARTFLSLDHLS
HHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHCC
GGRAGWNLVTSASPIEAANFGSTGLKPHADRYERAREFAGVVTGLWRGKASGHDGQSFSV
CCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
RDPLDLPRSPQGAPVMVQAGASDVGRDLAARTADVVFTAAQTFEEAKAFYDDLKGRMAAY
CCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GREPDDIKIMPGVSPVVAETEAEAREKHEELQVLIPDDVGVALLSSYLSISDLWRYPIDG
CCCCCCEEEECCCCCHHHCCHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCC
PLPELPESEGMKSRQALVVEQSRRDGLSIRQLARHFAGARGHWRIVGTAAQVADELQARF
CCCCCCCCCCCCHHHHHHEEHHCCCCCHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHH
EGGAADGFNVMPSYFPGELDAFATLVVPELQRRGLFRRDYEGRTLRDHLGLKRPV
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCC
>Mature Secondary Structure 
TAQMKLGAFLWATGHHIAAWRHPKAHVTAGIDIDHYIQLARTAEAAKFDMIFCEDAAGL
CCCEECCEEEEECCCEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHEEEEEECCCCCC
REANVGIASQTSRSIGFEPISLLSALAVQTSRIGLVSTASTSYNEPYGLARTFLSLDHLS
HHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHCC
GGRAGWNLVTSASPIEAANFGSTGLKPHADRYERAREFAGVVTGLWRGKASGHDGQSFSV
CCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
RDPLDLPRSPQGAPVMVQAGASDVGRDLAARTADVVFTAAQTFEEAKAFYDDLKGRMAAY
CCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GREPDDIKIMPGVSPVVAETEAEAREKHEELQVLIPDDVGVALLSSYLSISDLWRYPIDG
CCCCCCEEEECCCCCHHHCCHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCC
PLPELPESEGMKSRQALVVEQSRRDGLSIRQLARHFAGARGHWRIVGTAAQVADELQARF
CCCCCCCCCCCCHHHHHHEEHHCCCCCHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHH
EGGAADGFNVMPSYFPGELDAFATLVVPELQRRGLFRRDYEGRTLRDHLGLKRPV
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: an alkanesulfonate; O2; FMNH2 [C]

Specific reaction: an alkanesulfonate + O2 + FMNH2 = an aldehyde + SO3 + H2O + FMN [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8892809; 9023192; 1735711 [H]