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The map label for this gene is cocE [H]

Identifier: 13474275

GI number: 13474275

Start: 4064854

End: 4066839

Strand: Reverse

Name: cocE [H]

Synonym: mll5128

Alternate gene names: 13474275

Gene position: 4066839-4064854 (Counterclockwise)

Preceding gene: 13474276

Following gene: 13474274

Centisome position: 57.8

GC content: 63.44

Gene sequence:

>1986_bases
ATGAAAACCGTCACAGAATTTCCCCGCAAGGTCGTCGAATTCCCTGATATGGGCATCGTCATGCCGGATGGCTGCCGGCT
GTCGGCGCGGGTTTGGATGCCGGAGGATGCCGGCAACGATCCGGTGCCTGTCATCCTTGAGCATCTGCCTTACCGCAAGC
GCGATGGCACCATTTTTCGCGATCAACTGACACATCCCTATTTTGCCGGCCACGGCTACGCCTCGATCCGCGTCGACATG
CGCGGCAATGGCGATTCCGAAGGGCTGATGGACGACGAATATTCCGAGCAGGAATTGCAGGACGCCTGCGACGTCATCGC
CTGGGCGGTGGCTCAACCCTGGTGTAACGGCAATGTCGGCATGATGGGCATTTCCTGGGGCGGCTTCAACTGCCTGCAGG
TTGCGGCCAAGCAGCCGCCGGCGCTGAAGGCGGTGATCAGCCTGTGCTCGACCGTCGACCGCTACGCCGACGACATCCAC
TACAAGGGCGGCTGTCTGCTGATCGAGAATTTCGGCTGGGCCTCCACCATGCTGTCCTACTCGTCGCGGCCGCCGGACCC
GCTGATCGCCGGCGACAACAGATGGCGCGATCTGTGGCTGACCCGCCTGGAAAACCAGCCCTTCCTTTTGCCGCTGTGGC
TCAGCCACCAGCACCGTGACGCCTATTGGAAGCGCGGCTCGATCTGCGAGGATTTTTCGGCCGTCAAGGCCGCCGTTCTG
TCGATCGGCGGCTGGCATGACGGCTACCGCAACACGATCTCGAACCTCGTCAGCAACATTGAGGCACCGGTCAAGGGCAT
TGTCGGCCCCTGGATCCACAAATACCCGCACTATGCCGGCCCCCAGCCGGCCATCGGCTTCCTGCAGGAAGCGCTGCGCT
GGTGGGACCGCTGGCTGAAGGGCACCGAGACCGGCGTCGAGGACGATCCCGCCTACCGCGCCTACGTGATGGACAGCGTA
CGCCCGGCACGCTGGCATCCAGAGCGGCCCGGCCGCTGGGTGGCGGAACAACAATGGCCGTCACCCGCGATCAAGACCCG
GACCATCGAACTGCTGCCCAAGGGGGGCAGACCTGCCATCGTCGCCTCGCCGCAGACCTGCGGGCTCGCCGGCGGCGAGT
ATTTTCCGTTCACCTTCGGCCCGGAACTGCCGGGCGACCAGCGTCCCGACGATGCCTTGTCTGTCTGTTTCGACCAGCCG
GAGCTCACCGAGGCCATCGACATTGTCGGGGCGCCGGAAGTGGAGGTCAGGCTTTCTTCCGATCGCCCGCAGGCCAACAT
CGCGATCCGGCTCTGCGACGTGCATCCCGACGGCGCTTCGGAACTGATCTCCTATGGCGTGCTCAATTTGACGCATCGCG
ACTCGCACGAATTCCCGCAGGCCCTGGTGCCGGGCGAGACCGTCTCTGCGCGCGTCGTGCTCGATCAATGCGCCTACCGC
GTGCCGGCCGGCCACAGCCTGCGCATCGCCGTGTCGAACGCCTATTGGCCCATGATCTGGCCGTCGCCGGAGCCGGTGCA
GCTGACCTTGTCGGCGGCGACACTTGCCTTGCCGCTGCGGCCGCCGGCAACAGGCGACGAAGCGACGTTCGCCGAGCCAG
AAGGTGCTGCGCCTTGGGCGACCGAGACGGTCCGTGCAGCCAATTCCGAGCGCCATGTCGATCGCGATGAAAAGACCGGA
ATCGTCACGCTTTCCATAGCGGATGATTTCGGCGCGGTGCGCGATCTCGACCACGGCCTGGTCAATGGCAGCATCGCCCG
CGAAACATGGACGATCCATCCTGACAATCCATTGTCAGCCTCCGGGAAAACACACTGGACGCAGACACTGTCGCGAAATG
GATGGTCGGTGCGAACCGAGACATGGGCCGAGATGCGATCGGACGCACAAAACTTCATCGTCAGCGCCAGAATCGAAGCC
TATGAGGGCGAAAAAATCGTCTTCGAGCGCAATTTCGAGGAAAAGATTCCACGCGCGCTTGTTTGA

Upstream 100 bases:

>100_bases
TGAGGGTGTGCCGCAGCTAGCAGTACAGGTCGCATAATTCGCTGCTCCAGTCGCGAATGGCGATTGAAACTTGCCGCGCC
GCGGGGCGTTATGCGCACCC

Downstream 100 bases:

>100_bases
GCGCTGACACCGTTGGTCCAAATGCGACGTACGATTTACGCCACATGATGTCGCATTCGGTCTTGCTTACCGGTTTCCCT
TACGGTCATTATTGCCCTCA

Product: glutaryl 7-ACA acylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 661; Mature: 661

Protein sequence:

>661_residues
MKTVTEFPRKVVEFPDMGIVMPDGCRLSARVWMPEDAGNDPVPVILEHLPYRKRDGTIFRDQLTHPYFAGHGYASIRVDM
RGNGDSEGLMDDEYSEQELQDACDVIAWAVAQPWCNGNVGMMGISWGGFNCLQVAAKQPPALKAVISLCSTVDRYADDIH
YKGGCLLIENFGWASTMLSYSSRPPDPLIAGDNRWRDLWLTRLENQPFLLPLWLSHQHRDAYWKRGSICEDFSAVKAAVL
SIGGWHDGYRNTISNLVSNIEAPVKGIVGPWIHKYPHYAGPQPAIGFLQEALRWWDRWLKGTETGVEDDPAYRAYVMDSV
RPARWHPERPGRWVAEQQWPSPAIKTRTIELLPKGGRPAIVASPQTCGLAGGEYFPFTFGPELPGDQRPDDALSVCFDQP
ELTEAIDIVGAPEVEVRLSSDRPQANIAIRLCDVHPDGASELISYGVLNLTHRDSHEFPQALVPGETVSARVVLDQCAYR
VPAGHSLRIAVSNAYWPMIWPSPEPVQLTLSAATLALPLRPPATGDEATFAEPEGAAPWATETVRAANSERHVDRDEKTG
IVTLSIADDFGAVRDLDHGLVNGSIARETWTIHPDNPLSASGKTHWTQTLSRNGWSVRTETWAEMRSDAQNFIVSARIEA
YEGEKIVFERNFEEKIPRALV

Sequences:

>Translated_661_residues
MKTVTEFPRKVVEFPDMGIVMPDGCRLSARVWMPEDAGNDPVPVILEHLPYRKRDGTIFRDQLTHPYFAGHGYASIRVDM
RGNGDSEGLMDDEYSEQELQDACDVIAWAVAQPWCNGNVGMMGISWGGFNCLQVAAKQPPALKAVISLCSTVDRYADDIH
YKGGCLLIENFGWASTMLSYSSRPPDPLIAGDNRWRDLWLTRLENQPFLLPLWLSHQHRDAYWKRGSICEDFSAVKAAVL
SIGGWHDGYRNTISNLVSNIEAPVKGIVGPWIHKYPHYAGPQPAIGFLQEALRWWDRWLKGTETGVEDDPAYRAYVMDSV
RPARWHPERPGRWVAEQQWPSPAIKTRTIELLPKGGRPAIVASPQTCGLAGGEYFPFTFGPELPGDQRPDDALSVCFDQP
ELTEAIDIVGAPEVEVRLSSDRPQANIAIRLCDVHPDGASELISYGVLNLTHRDSHEFPQALVPGETVSARVVLDQCAYR
VPAGHSLRIAVSNAYWPMIWPSPEPVQLTLSAATLALPLRPPATGDEATFAEPEGAAPWATETVRAANSERHVDRDEKTG
IVTLSIADDFGAVRDLDHGLVNGSIARETWTIHPDNPLSASGKTHWTQTLSRNGWSVRTETWAEMRSDAQNFIVSARIEA
YEGEKIVFERNFEEKIPRALV
>Mature_661_residues
MKTVTEFPRKVVEFPDMGIVMPDGCRLSARVWMPEDAGNDPVPVILEHLPYRKRDGTIFRDQLTHPYFAGHGYASIRVDM
RGNGDSEGLMDDEYSEQELQDACDVIAWAVAQPWCNGNVGMMGISWGGFNCLQVAAKQPPALKAVISLCSTVDRYADDIH
YKGGCLLIENFGWASTMLSYSSRPPDPLIAGDNRWRDLWLTRLENQPFLLPLWLSHQHRDAYWKRGSICEDFSAVKAAVL
SIGGWHDGYRNTISNLVSNIEAPVKGIVGPWIHKYPHYAGPQPAIGFLQEALRWWDRWLKGTETGVEDDPAYRAYVMDSV
RPARWHPERPGRWVAEQQWPSPAIKTRTIELLPKGGRPAIVASPQTCGLAGGEYFPFTFGPELPGDQRPDDALSVCFDQP
ELTEAIDIVGAPEVEVRLSSDRPQANIAIRLCDVHPDGASELISYGVLNLTHRDSHEFPQALVPGETVSARVVLDQCAYR
VPAGHSLRIAVSNAYWPMIWPSPEPVQLTLSAATLALPLRPPATGDEATFAEPEGAAPWATETVRAANSERHVDRDEKTG
IVTLSIADDFGAVRDLDHGLVNGSIARETWTIHPDNPLSASGKTHWTQTLSRNGWSVRTETWAEMRSDAQNFIVSARIEA
YEGEKIVFERNFEEKIPRALV

Specific function: Hydrolyzes cocaine to benzoate and ecgonine methyl ester, endowing the bacteria with the ability to utilize cocaine as a sole source of carbon and energy for growth, as this bacterium lives in the rhizosphere of coca plants [H]

COG id: COG2936

COG function: function code R; Predicted acyl esterases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha/beta hydrolase family. Carboxylesterase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005674
- InterPro:   IPR008979
- InterPro:   IPR013736
- InterPro:   IPR000383 [H]

Pfam domain/function: PF02129 Peptidase_S15; PF08530 PepX_C [H]

EC number: NA

Molecular weight: Translated: 73570; Mature: 73570

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTVTEFPRKVVEFPDMGIVMPDGCRLSARVWMPEDAGNDPVPVILEHLPYRKRDGTIFR
CCCHHHHHHHHHCCCCCCEECCCCCEEEEEEECCCCCCCCCHHHHHHHCCCCCCCCCEEH
DQLTHPYFAGHGYASIRVDMRGNGDSEGLMDDEYSEQELQDACDVIAWAVAQPWCNGNVG
HHCCCCEECCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEE
MMGISWGGFNCLQVAAKQPPALKAVISLCSTVDRYADDIHYKGGCLLIENFGWASTMLSY
EEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCEECCCEEEEECCCHHHHHHHH
SSRPPDPLIAGDNRWRDLWLTRLENQPFLLPLWLSHQHRDAYWKRGSICEDFSAVKAAVL
CCCCCCCEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHCCCCCHHHHHHHHHHHH
SIGGWHDGYRNTISNLVSNIEAPVKGIVGPWIHKYPHYAGPQPAIGFLQEALRWWDRWLK
HCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHC
GTETGVEDDPAYRAYVMDSVRPARWHPERPGRWVAEQQWPSPAIKTRTIELLPKGGRPAI
CCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCEE
VASPQTCGLAGGEYFPFTFGPELPGDQRPDDALSVCFDQPELTEAIDIVGAPEVEVRLSS
EECCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHCCCCHHHHHHHHCCCCCEEEEECC
DRPQANIAIRLCDVHPDGASELISYGVLNLTHRDSHEFPQALVPGETVSARVVLDQCAYR
CCCCCEEEEEEEECCCCCHHHHHHHHHEEEECCCCCCCCHHHCCCCCCCHHEEHHHHHHC
VPAGHSLRIAVSNAYWPMIWPSPEPVQLTLSAATLALPLRPPATGDEATFAEPEGAAPWA
CCCCCEEEEEEECCCCCEECCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCH
TETVRAANSERHVDRDEKTGIVTLSIADDFGAVRDLDHGLVNGSIARETWTIHPDNPLSA
HHHHHHCCCHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEECCEEEECCCCCCCC
SGKTHWTQTLSRNGWSVRTETWAEMRSDAQNFIVSARIEAYEGEKIVFERNFEEKIPRAL
CCCCHHHHHHHCCCCEEEHHHHHHHHHHHHHEEEEEEEEEECCCEEEEECCHHHHCCHHC
V
C
>Mature Secondary Structure
MKTVTEFPRKVVEFPDMGIVMPDGCRLSARVWMPEDAGNDPVPVILEHLPYRKRDGTIFR
CCCHHHHHHHHHCCCCCCEECCCCCEEEEEEECCCCCCCCCHHHHHHHCCCCCCCCCEEH
DQLTHPYFAGHGYASIRVDMRGNGDSEGLMDDEYSEQELQDACDVIAWAVAQPWCNGNVG
HHCCCCEECCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEE
MMGISWGGFNCLQVAAKQPPALKAVISLCSTVDRYADDIHYKGGCLLIENFGWASTMLSY
EEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCEECCCEEEEECCCHHHHHHHH
SSRPPDPLIAGDNRWRDLWLTRLENQPFLLPLWLSHQHRDAYWKRGSICEDFSAVKAAVL
CCCCCCCEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCHHHHCCCCCHHHHHHHHHHHH
SIGGWHDGYRNTISNLVSNIEAPVKGIVGPWIHKYPHYAGPQPAIGFLQEALRWWDRWLK
HCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHC
GTETGVEDDPAYRAYVMDSVRPARWHPERPGRWVAEQQWPSPAIKTRTIELLPKGGRPAI
CCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCEE
VASPQTCGLAGGEYFPFTFGPELPGDQRPDDALSVCFDQPELTEAIDIVGAPEVEVRLSS
EECCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHCCCCHHHHHHHHCCCCCEEEEECC
DRPQANIAIRLCDVHPDGASELISYGVLNLTHRDSHEFPQALVPGETVSARVVLDQCAYR
CCCCCEEEEEEEECCCCCHHHHHHHHHEEEECCCCCCCCHHHCCCCCCCHHEEHHHHHHC
VPAGHSLRIAVSNAYWPMIWPSPEPVQLTLSAATLALPLRPPATGDEATFAEPEGAAPWA
CCCCCEEEEEEECCCCCEECCCCCCEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCH
TETVRAANSERHVDRDEKTGIVTLSIADDFGAVRDLDHGLVNGSIARETWTIHPDNPLSA
HHHHHHCCCHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEECCEEEECCCCCCCC
SGKTHWTQTLSRNGWSVRTETWAEMRSDAQNFIVSARIEAYEGEKIVFERNFEEKIPRAL
CCCCHHHHHHHCCCCEEEHHHHHHHHHHHHHEEEEEEEEEECCCEEEEECCHHHHCCHHC
V
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10698749; 11742345; 12369817 [H]