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The map label for this gene is trmE

Identifier: 13473776

GI number: 13473776

Start: 3566016

End: 3567347

Strand: Reverse

Name: trmE

Synonym: mll4483

Alternate gene names: 13473776

Gene position: 3567347-3566016 (Counterclockwise)

Preceding gene: 13473778

Following gene: 13473775

Centisome position: 50.7

GC content: 64.64

Gene sequence:

>1332_bases
ATGATTTCAGGCGATTCGATCGTTGCGCTGTCGAGCGGCCGGCTTCCGGCCGGTGTCGCCGTGCTGCGTATCTCCGGTCC
GCAGACTCGATTCGTGGTCGAAACGATTGCTGGTGGCATGGTTAAGGACCGGGTGGCGGTCTTGCGCAGATTCAAGGCGC
CGGATGGAACCGTGCTCGACAGCGGCTTGGTCATCTTCTTTCCCGGTCCGGCCAGCTTTACCGGGGAGGATGTCGCCGAA
TTCCATGTCCATGGCGGACGCGCCGTCGTGGCGCGGATGCTGGAGATCATCAGTGGTTTCGACGGCGTCAGGCACGCCGA
ACCCGGCGAATTCACGCGCCGCGCCTTTCTCAACGGCAAGGTCGACCTGGTCGAGACGGAAGCACTGGCGGATCTGGTCA
ATGCCGAGACCGAGGCGCAGCGACGCTTCGCGGTCCGCAATGCCGAGGGTGTCCAGAGCGAGCTCTATCTGAGCTGGCGC
CGCCGACTGATCCATGCGCGGGCGATGATCGAGGCCGAGATCGATTTTGCTGATGAAGATGACGTGCCAGGATCCGTTTC
GGATACGGTCTGGTCTGATGTCCGGGCGATGATTGGCGAGATCGATCGGCATATCGCCGGATTTCACGCCGCCGAGATCA
TCCGCGAAGGCTTCGAGGTGGTGATTCTCGGCGCGCCGAATGCCGGCAAGTCGAGTCTGTTCAACGCCTTGGCGCGGCGC
GATGCAGCTATTGTAACGGATGAGCCGGGCACGACGCGGGACCTACTCGAGGTGACGCTGGATCTCGGCGGCCTGCGTGT
CAGGCTGACGGACACGGCCGGCTTGCGCGAAGCGCCGGGTAAGGTCGAGGCGATCGGCATCGAGAAGGCGCGGGCCAAGG
CCGACCGCGCCGATCTCTTGCTGCTGCTGGAGGACATTTTGGCGCCCGGGGTCCTTGGGCCGTTGCCGGGCAAGGCGCCT
CTTTTGCGGGTCGGCACCAAACTCGACCTGCTGGATGAGGGGTCGGCTCGGGAGGCGGCCGGTCGCTACGACGTTGCCAT
TTCTGTTGTAGGCGGGACTGGCGTGGAAGCGCTGCTGGCTGAAATCGGCCGTCGCGCGGCGGATGCCGCCGGCGACGTCG
GCGATGTTCTGCCGTCGCGCCTGCGCCATGTCGAACTGCTCGGTGAGGCGAATCGCCATCTGCTTCGCGCCGCCGCCGAG
GACGCAGCAGGTCAGGAACTGCGCGCCGAAGAGCTGCGACTGGCGGCGGACAGCCTGGGTCGAATCGTCGGCGCGATCGA
TGTCGAGGACATGCTGGACGTGATCTTTTCGCAGTTCTGCATCGGGAAATGA

Upstream 100 bases:

>100_bases
CTGGCTTCTTCGGCAAGCAGGTTTTGCGCGGGCAATGTCACAATGGGCTACTCGGGTTGTTTGAAGGCGCGAGGCTAGGG
CGGTTCAACAGAGCGGGCAA

Downstream 100 bases:

>100_bases
CTCACGTGAAACAATGACTTGGGCGAACACGCCGGGGATCTCCGTGGTGGTGATGCATTTGCGGAAACGCCTGTCACTTC
GTCATCCTAGGGCGAAGCAA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MISGDSIVALSSGRLPAGVAVLRISGPQTRFVVETIAGGMVKDRVAVLRRFKAPDGTVLDSGLVIFFPGPASFTGEDVAE
FHVHGGRAVVARMLEIISGFDGVRHAEPGEFTRRAFLNGKVDLVETEALADLVNAETEAQRRFAVRNAEGVQSELYLSWR
RRLIHARAMIEAEIDFADEDDVPGSVSDTVWSDVRAMIGEIDRHIAGFHAAEIIREGFEVVILGAPNAGKSSLFNALARR
DAAIVTDEPGTTRDLLEVTLDLGGLRVRLTDTAGLREAPGKVEAIGIEKARAKADRADLLLLLEDILAPGVLGPLPGKAP
LLRVGTKLDLLDEGSAREAAGRYDVAISVVGGTGVEALLAEIGRRAADAAGDVGDVLPSRLRHVELLGEANRHLLRAAAE
DAAGQELRAEELRLAADSLGRIVGAIDVEDMLDVIFSQFCIGK

Sequences:

>Translated_443_residues
MISGDSIVALSSGRLPAGVAVLRISGPQTRFVVETIAGGMVKDRVAVLRRFKAPDGTVLDSGLVIFFPGPASFTGEDVAE
FHVHGGRAVVARMLEIISGFDGVRHAEPGEFTRRAFLNGKVDLVETEALADLVNAETEAQRRFAVRNAEGVQSELYLSWR
RRLIHARAMIEAEIDFADEDDVPGSVSDTVWSDVRAMIGEIDRHIAGFHAAEIIREGFEVVILGAPNAGKSSLFNALARR
DAAIVTDEPGTTRDLLEVTLDLGGLRVRLTDTAGLREAPGKVEAIGIEKARAKADRADLLLLLEDILAPGVLGPLPGKAP
LLRVGTKLDLLDEGSAREAAGRYDVAISVVGGTGVEALLAEIGRRAADAAGDVGDVLPSRLRHVELLGEANRHLLRAAAE
DAAGQELRAEELRLAADSLGRIVGAIDVEDMLDVIFSQFCIGK
>Mature_443_residues
MISGDSIVALSSGRLPAGVAVLRISGPQTRFVVETIAGGMVKDRVAVLRRFKAPDGTVLDSGLVIFFPGPASFTGEDVAE
FHVHGGRAVVARMLEIISGFDGVRHAEPGEFTRRAFLNGKVDLVETEALADLVNAETEAQRRFAVRNAEGVQSELYLSWR
RRLIHARAMIEAEIDFADEDDVPGSVSDTVWSDVRAMIGEIDRHIAGFHAAEIIREGFEVVILGAPNAGKSSLFNALARR
DAAIVTDEPGTTRDLLEVTLDLGGLRVRLTDTAGLREAPGKVEAIGIEKARAKADRADLLLLLEDILAPGVLGPLPGKAP
LLRVGTKLDLLDEGSAREAAGRYDVAISVVGGTGVEALLAEIGRRAADAAGDVGDVLPSRLRHVELLGEANRHLLRAAAE
DAAGQELRAEELRLAADSLGRIVGAIDVEDMLDVIFSQFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=470, Percent_Identity=40.8510638297872, Blast_Score=284, Evalue=9e-77,
Organism=Homo sapiens, GI193082993, Length=454, Percent_Identity=41.1894273127753, Blast_Score=278, Evalue=5e-75,
Organism=Homo sapiens, GI193082988, Length=502, Percent_Identity=38.6454183266932, Blast_Score=273, Evalue=3e-73,
Organism=Escherichia coli, GI2367268, Length=456, Percent_Identity=40.7894736842105, Blast_Score=278, Evalue=4e-76,
Organism=Caenorhabditis elegans, GI17507259, Length=456, Percent_Identity=32.4561403508772, Blast_Score=210, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6323665, Length=491, Percent_Identity=33.1975560081466, Blast_Score=251, Evalue=2e-67,
Organism=Drosophila melanogaster, GI45550813, Length=474, Percent_Identity=40.084388185654, Blast_Score=297, Evalue=8e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_RHILO (Q98DZ0)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_105344.1
- HSSP:   P25522
- ProteinModelPortal:   Q98DZ0
- SMR:   Q98DZ0
- GeneID:   1228005
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll4483
- NMPDR:   fig|266835.1.peg.3448
- HOGENOM:   HBG746795
- OMA:   LIDNAMV
- ProtClustDB:   PRK05291
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00449
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 47171; Mature: 47171

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: BINDING 23-23 BINDING 80-80 BINDING 120-120 BINDING 443-443

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISGDSIVALSSGRLPAGVAVLRISGPQTRFVVETIAGGMVKDRVAVLRRFKAPDGTVLD
CCCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEC
SGLVIFFPGPASFTGEDVAEFHVHGGRAVVARMLEIISGFDGVRHAEPGEFTRRAFLNGK
CCEEEEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCC
VDLVETEALADLVNAETEAQRRFAVRNAEGVQSELYLSWRRRLIHARAMIEAEIDFADED
EEEEHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCC
DVPGSVSDTVWSDVRAMIGEIDRHIAGFHAAEIIREGFEVVILGAPNAGKSSLFNALARR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHC
DAAIVTDEPGTTRDLLEVTLDLGGLRVRLTDTAGLREAPGKVEAIGIEKARAKADRADLL
CCEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEECHHHHHHCCHHHHH
LLLEDILAPGVLGPLPGKAPLLRVGTKLDLLDEGSAREAAGRYDVAISVVGGTGVEALLA
HHHHHHHCCCCCCCCCCCCCEEEECCEEEECCCCCCHHCCCCEEEEEEEECCCCHHHHHH
EIGRRAADAAGDVGDVLPSRLRHVELLGEANRHLLRAAAEDAAGQELRAEELRLAADSLG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RIVGAIDVEDMLDVIFSQFCIGK
HHHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MISGDSIVALSSGRLPAGVAVLRISGPQTRFVVETIAGGMVKDRVAVLRRFKAPDGTVLD
CCCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEC
SGLVIFFPGPASFTGEDVAEFHVHGGRAVVARMLEIISGFDGVRHAEPGEFTRRAFLNGK
CCEEEEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCC
VDLVETEALADLVNAETEAQRRFAVRNAEGVQSELYLSWRRRLIHARAMIEAEIDFADED
EEEEHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCC
DVPGSVSDTVWSDVRAMIGEIDRHIAGFHAAEIIREGFEVVILGAPNAGKSSLFNALARR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHC
DAAIVTDEPGTTRDLLEVTLDLGGLRVRLTDTAGLREAPGKVEAIGIEKARAKADRADLL
CCEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEECHHHHHHCCHHHHH
LLLEDILAPGVLGPLPGKAPLLRVGTKLDLLDEGSAREAAGRYDVAISVVGGTGVEALLA
HHHHHHHCCCCCCCCCCCCCEEEECCEEEECCCCCCHHCCCCEEEEEEEECCCCHHHHHH
EIGRRAADAAGDVGDVLPSRLRHVELLGEANRHLLRAAAEDAAGQELRAEELRLAADSLG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RIVGAIDVEDMLDVIFSQFCIGK
HHHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968