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The map label for this gene is leuD

Identifier: 13473715

GI number: 13473715

Start: 3494905

End: 3495510

Strand: Reverse

Name: leuD

Synonym: mll4408

Alternate gene names: 13473715

Gene position: 3495510-3494905 (Counterclockwise)

Preceding gene: 13473718

Following gene: 13473714

Centisome position: 49.68

GC content: 60.89

Gene sequence:

>606_bases
ATGGAAAAATTCACCAAGCTCACCGGCGTCGCCGCTCCGATGCCGATCGTCAATGTCGACACCGACATGATCATCCCGAA
GGATTATCTCAAGACGATCAAGCGCACCGGCCTCGGCACCGGCCTGTTCGCCGAGATGCGCTACAATGACGACGGTTCGG
AAAATCCGGATTTCGTGCTCAACAAGCCGGCCTACCGCAAGGCGCAGATCCTGGTCGCCGGCGACAATTTCGGCTGCGGC
TCGAGCCGCGAGCACGCGCCGTGGGCGCTGCTCGATTTCGGCATTCGCTGCGTCATCTCGACCTCGTTCGCCGACATTTT
CTACAACAACTGCTTCAAGAACGGCGTGCTGCCGATCACCGTCAGCCCCGAGGATCTCGAGAAGCTGATGGATGACGCCT
CGCGCGGCTCCAACGCCACTTTGTCGGTTGACCTCGAAGCCAAGGAAATCCGCGGCCCCGATGGCGGCGTGGTCAAGTTC
GATCTCGACGACTTCAAGCGGCACTGCATGCTCAACGGGCTCGACGATATCGGCCTGACCATGGAGAAGGCCGGCGCCAT
CGCCTCGTTCGAGAAGAAAAACGCCGAACAGCGCCCCTGGGCCTGA

Upstream 100 bases:

>100_bases
TTGTGCCAGCGCGCACGAACAGTTGCCTTTTATTTGCCCTCGGCTTTGTGTAAACGGGGCTTCAGCAAATCCCCCCGCAA
CGAAAACACGGGCCAAAGCC

Downstream 100 bases:

>100_bases
CCGGCACCATCCGACGATAGGCCCGGCCGCGTTCCGGCCGGGTTTGGTGGCGACTGAGGGAGGGACAGTCATGGCACGTT
CGCTTCTTGCCGGCGTTTGC

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MEKFTKLTGVAAPMPIVNVDTDMIIPKDYLKTIKRTGLGTGLFAEMRYNDDGSENPDFVLNKPAYRKAQILVAGDNFGCG
SSREHAPWALLDFGIRCVISTSFADIFYNNCFKNGVLPITVSPEDLEKLMDDASRGSNATLSVDLEAKEIRGPDGGVVKF
DLDDFKRHCMLNGLDDIGLTMEKAGAIASFEKKNAEQRPWA

Sequences:

>Translated_201_residues
MEKFTKLTGVAAPMPIVNVDTDMIIPKDYLKTIKRTGLGTGLFAEMRYNDDGSENPDFVLNKPAYRKAQILVAGDNFGCG
SSREHAPWALLDFGIRCVISTSFADIFYNNCFKNGVLPITVSPEDLEKLMDDASRGSNATLSVDLEAKEIRGPDGGVVKF
DLDDFKRHCMLNGLDDIGLTMEKAGAIASFEKKNAEQRPWA
>Mature_201_residues
MEKFTKLTGVAAPMPIVNVDTDMIIPKDYLKTIKRTGLGTGLFAEMRYNDDGSENPDFVLNKPAYRKAQILVAGDNFGCG
SSREHAPWALLDFGIRCVISTSFADIFYNNCFKNGVLPITVSPEDLEKLMDDASRGSNATLSVDLEAKEIRGPDGGVVKF
DLDDFKRHCMLNGLDDIGLTMEKAGAIASFEKKNAEQRPWA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=195, Percent_Identity=49.7435897435897, Blast_Score=202, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6321429, Length=204, Percent_Identity=50.9803921568627, Blast_Score=200, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_RHILO (Q98E51)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_105283.1
- ProteinModelPortal:   Q98E51
- SMR:   Q98E51
- GeneID:   1227944
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll4408
- NMPDR:   fig|266835.1.peg.3387
- HOGENOM:   HBG304838
- OMA:   DEISITM
- ProtClustDB:   PRK01641
- BRENDA:   4.2.1.33
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 22117; Mature: 22117

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKFTKLTGVAAPMPIVNVDTDMIIPKDYLKTIKRTGLGTGLFAEMRYNDDGSENPDFVL
CCCHHHHHCCCCCCCEEECCCCEECCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEE
NKPAYRKAQILVAGDNFGCGSSREHAPWALLDFGIRCVISTSFADIFYNNCFKNGVLPIT
CCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHCEEEEEEHHHHHHHHHHHHCCCEEEEE
VSPEDLEKLMDDASRGSNATLSVDLEAKEIRGPDGGVVKFDLDDFKRHCMLNGLDDIGLT
ECHHHHHHHHHHHCCCCCCEEEEEEEHHHCCCCCCCEEEEEHHHHHHHHHHCCCCCCCEE
MEKAGAIASFEKKNAEQRPWA
HHHHCCHHHHHHCCCCCCCCC
>Mature Secondary Structure
MEKFTKLTGVAAPMPIVNVDTDMIIPKDYLKTIKRTGLGTGLFAEMRYNDDGSENPDFVL
CCCHHHHHCCCCCCCEEECCCCEECCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEE
NKPAYRKAQILVAGDNFGCGSSREHAPWALLDFGIRCVISTSFADIFYNNCFKNGVLPIT
CCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHCEEEEEEHHHHHHHHHHHHCCCEEEEE
VSPEDLEKLMDDASRGSNATLSVDLEAKEIRGPDGGVVKFDLDDFKRHCMLNGLDDIGLT
ECHHHHHHHHHHHCCCCCCEEEEEEEHHHCCCCCCCEEEEEHHHHHHHHHHCCCCCCCEE
MEKAGAIASFEKKNAEQRPWA
HHHHCCHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968