Click here to switch to the map view.

The map label for this gene is rlmN

Identifier: 13473680

GI number: 13473680

Start: 3463983

End: 3465215

Strand: Direct

Name: rlmN

Synonym: mlr4359

Alternate gene names: 13473680

Gene position: 3463983-3465215 (Clockwise)

Preceding gene: 13473677

Following gene: 13473681

Centisome position: 49.23

GC content: 64.23

Gene sequence:

>1233_bases
ATGACCCTTTCCTTCGACCTCACCACTGAAGGTGCCCGTGACGCGTTGCGCGCCCGCGCCGCGCCCGAGAAGCCGTCGCT
GATCGGCCTGACGCGCGCCGAGCTTGGCGAGGCGCTGGTCGCGTCCGGCATCGTGCCGGAGCGCCAGGCCAAGATGCGCG
CCCAGCAGCTCTGGCACTGGATGTATGTGCGCGGCGTTTCCGACTTCGCCGGCATGTTCAACATCTCCAAGGACCTGCGC
GCCGAGCTCGACAAGCATTTCACCGTTGCCCGTCCCGAAATCGTCGAGGAGCAGATCTCTTCCGACGGGACCCGCAAATG
GCTGTTCCGCTTTCCGCCGCGCGGCGCCGGCCGGCCGGTCGAGATCGAGACCGTCTACATCCCCGAGGAAGGCCGCGGCA
CGCTCTGCATCTCCTCTCAGGTCGGCTGCACGCTGACCTGCTCGTTCTGCCACACAGGCACGCAGAAACTGGTGCGCAAC
CTCACCACTGAGGAAATCCTTGCCCAGCTGCTCACCGCCCGCGACCGGCTGGGCGACTTCCCCGACCGCGACACGCCGGA
CGGCGCCATCGTGCCGGCCGAGGGACGCAAGGTGTCCAACATCGTCATGATGGGCATGGGCGAACCGCTCTACAATTTCG
AGGCGGTGAAGAAGGCGCTGCTGATCGCTTCCGACGGCGACGGGCTTTCCCTGTCGAAGCGCCGCATCACACTGTCGACC
TCCGGCGTCGTGCCCGAGATTTTCCGCACCGGCGAGGAGATCGGCGTCATGCTGGCGATCTCGCTGCATGCCACCAATGA
CGATCTGCGTGACCTTTTGGTGCCGATCAACAAGAAGTATCCGCTGAAGGAGCTGATTGCCGCCTGCCGCGCCTATCCCG
GCCTGTCGAACGCCAAGCGCATCACTTTCGAATATGTGATGCTGAAGGACGTCAACGATTCCATCGAGGACGCCAAGGGC
CTGATCAAGCTGCTCAAGGGCATTCCGGCCAAGATCAATCTGATCCCGTTCAACCCGTGGCCGGGCACCAACTACCAGTG
CTCGGACTGGGAGACGATCGAGAAATTCGCCGACTACATCAACAATGCCGGCTATGCCTCGCCGATCCGCACACCGCGCG
GCCGCGACATCCTCGCCGCCTGCGGCCAGCTGAAATCGGAATCCGAGCGCATGCGAAAAGTGGACCGGCTGGCGCTGGAA
GCCATGATGATCGCGGGGCACGGGGAGGCGTGA

Upstream 100 bases:

>100_bases
TCCCCGGCATGACTCTGCCCTCAACCTGTGCTATGCGCCGCGCTTCAGTTCCCGGCACGATGGATGAAATCGGCCGCAAA
CCGCTTATATCAGTGCGATC

Downstream 100 bases:

>100_bases
GCCGTCTCGTCGCCGGGGTCAAACGCATCGCCATCATAGCCGTCGGTTATATCGCCGGTGTGCTGACCTCGATCCTGGTG
CCCGTCCTGCTGATCACACT

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MTLSFDLTTEGARDALRARAAPEKPSLIGLTRAELGEALVASGIVPERQAKMRAQQLWHWMYVRGVSDFAGMFNISKDLR
AELDKHFTVARPEIVEEQISSDGTRKWLFRFPPRGAGRPVEIETVYIPEEGRGTLCISSQVGCTLTCSFCHTGTQKLVRN
LTTEEILAQLLTARDRLGDFPDRDTPDGAIVPAEGRKVSNIVMMGMGEPLYNFEAVKKALLIASDGDGLSLSKRRITLST
SGVVPEIFRTGEEIGVMLAISLHATNDDLRDLLVPINKKYPLKELIAACRAYPGLSNAKRITFEYVMLKDVNDSIEDAKG
LIKLLKGIPAKINLIPFNPWPGTNYQCSDWETIEKFADYINNAGYASPIRTPRGRDILAACGQLKSESERMRKVDRLALE
AMMIAGHGEA

Sequences:

>Translated_410_residues
MTLSFDLTTEGARDALRARAAPEKPSLIGLTRAELGEALVASGIVPERQAKMRAQQLWHWMYVRGVSDFAGMFNISKDLR
AELDKHFTVARPEIVEEQISSDGTRKWLFRFPPRGAGRPVEIETVYIPEEGRGTLCISSQVGCTLTCSFCHTGTQKLVRN
LTTEEILAQLLTARDRLGDFPDRDTPDGAIVPAEGRKVSNIVMMGMGEPLYNFEAVKKALLIASDGDGLSLSKRRITLST
SGVVPEIFRTGEEIGVMLAISLHATNDDLRDLLVPINKKYPLKELIAACRAYPGLSNAKRITFEYVMLKDVNDSIEDAKG
LIKLLKGIPAKINLIPFNPWPGTNYQCSDWETIEKFADYINNAGYASPIRTPRGRDILAACGQLKSESERMRKVDRLALE
AMMIAGHGEA
>Mature_409_residues
TLSFDLTTEGARDALRARAAPEKPSLIGLTRAELGEALVASGIVPERQAKMRAQQLWHWMYVRGVSDFAGMFNISKDLRA
ELDKHFTVARPEIVEEQISSDGTRKWLFRFPPRGAGRPVEIETVYIPEEGRGTLCISSQVGCTLTCSFCHTGTQKLVRNL
TTEEILAQLLTARDRLGDFPDRDTPDGAIVPAEGRKVSNIVMMGMGEPLYNFEAVKKALLIASDGDGLSLSKRRITLSTS
GVVPEIFRTGEEIGVMLAISLHATNDDLRDLLVPINKKYPLKELIAACRAYPGLSNAKRITFEYVMLKDVNDSIEDAKGL
IKLLKGIPAKINLIPFNPWPGTNYQCSDWETIEKFADYINNAGYASPIRTPRGRDILAACGQLKSESERMRKVDRLALEA
MMIAGHGEA

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=363, Percent_Identity=46.831955922865, Blast_Score=292, Evalue=2e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_RHILO (Q98E86)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_105248.1
- ProteinModelPortal:   Q98E86
- GeneID:   1227909
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr4359
- NMPDR:   fig|266835.1.peg.3352
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   CLSK864022
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 45341; Mature: 45210

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSFDLTTEGARDALRARAAPEKPSLIGLTRAELGEALVASGIVPERQAKMRAQQLWHW
CEEEEECCCCHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
MYVRGVSDFAGMFNISKDLRAELDKHFTVARPEIVEEQISSDGTRKWLFRFPPRGAGRPV
HHHHHHHHHHHHHCCCHHHHHHHHHCEECCCHHHHHHHHCCCCCEEEEEECCCCCCCCCE
EIETVYIPEEGRGTLCISSQVGCTLTCSFCHTGTQKLVRNLTTEEILAQLLTARDRLGDF
EEEEEEECCCCCCEEEEECCCCCEEEEHHHHCCHHHHHHCCCHHHHHHHHHHHHHHCCCC
PDRDTPDGAIVPAEGRKVSNIVMMGMGEPLYNFEAVKKALLIASDGDGLSLSKRRITLST
CCCCCCCCCEECCCCCEEEEEEEEECCCCCHHHHHHHHEEEEECCCCCCEEECEEEEEEC
SGVVPEIFRTGEEIGVMLAISLHATNDDLRDLLVPINKKYPLKELIAACRAYPGLSNAKR
CCCCHHHHHCCCCCEEEEEEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCE
ITFEYVMLKDVNDSIEDAKGLIKLLKGIPAKINLIPFNPWPGTNYQCSDWETIEKFADYI
EEEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHH
NNAGYASPIRTPRGRDILAACGQLKSESERMRKVDRLALEAMMIAGHGEA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCC
>Mature Secondary Structure 
TLSFDLTTEGARDALRARAAPEKPSLIGLTRAELGEALVASGIVPERQAKMRAQQLWHW
EEEEECCCCHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
MYVRGVSDFAGMFNISKDLRAELDKHFTVARPEIVEEQISSDGTRKWLFRFPPRGAGRPV
HHHHHHHHHHHHHCCCHHHHHHHHHCEECCCHHHHHHHHCCCCCEEEEEECCCCCCCCCE
EIETVYIPEEGRGTLCISSQVGCTLTCSFCHTGTQKLVRNLTTEEILAQLLTARDRLGDF
EEEEEEECCCCCCEEEEECCCCCEEEEHHHHCCHHHHHHCCCHHHHHHHHHHHHHHCCCC
PDRDTPDGAIVPAEGRKVSNIVMMGMGEPLYNFEAVKKALLIASDGDGLSLSKRRITLST
CCCCCCCCCEECCCCCEEEEEEEEECCCCCHHHHHHHHEEEEECCCCCCEEECEEEEEEC
SGVVPEIFRTGEEIGVMLAISLHATNDDLRDLLVPINKKYPLKELIAACRAYPGLSNAKR
CCCCHHHHHCCCCCEEEEEEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCE
ITFEYVMLKDVNDSIEDAKGLIKLLKGIPAKINLIPFNPWPGTNYQCSDWETIEKFADYI
EEEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHH
NNAGYASPIRTPRGRDILAACGQLKSESERMRKVDRLALEAMMIAGHGEA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968