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The map label for this gene is dapF

Identifier: 13473650

GI number: 13473650

Start: 3436326

End: 3437210

Strand: Direct

Name: dapF

Synonym: mlr4318

Alternate gene names: 13473650

Gene position: 3436326-3437210 (Clockwise)

Preceding gene: 13473649

Following gene: 13473651

Centisome position: 48.84

GC content: 66.33

Gene sequence:

>885_bases
ATGGCAAGCACTGCCCCTTTCGCCAAGATGAACGGCATCGGCAACGAGATCATCGTCGCCGATATGCGCGGCCGTGCCGA
TCAGGTGACGGCGGCCGCCGCCCTTGCGCTGAACGGAGATGCCGCGACCCGCTTCGACCAGATCATGGCGATCCATGATG
CGAGGACGCCGGGCACCGCCTACTATGTCGACATCCTGAATTCCGACGGCACCAGTGCACAGGCCTGCGGCAATGGCATG
CGTTGCGTCGTCCAGGCGCTCGCCGCCGAGACCGGGCAAAAGACCTTCACCTTCGAGACCCGCGCCGGCATTCTCAATGC
CCGGGAGCATGCCGACGGCACGATCTCGGTCGACATGGGCATGCCGCATTTCGGCTGGCAGGAGATCCCGCTCGCCGAGG
AGTTCCGCGACACCCGCATGATCGAATTGCAGATCGGCCCGATCGACGCGCCGGTGCTGCATTCGCCCTCAGCCGTCTCG
ATGGGCAACCCGCACGCCATCTTCTGGGTCGACCGCGACGTCTGGTCCTATGAGCTCGAACGTTTCGGCCCGCTGCTCGA
AAACCACCCGATCTTTCCCGAGCGCGCCAACATCACCATCGCCCAGGTGACCTCGCCCGAGAGCATGATCATCCGCACCT
GGGAACGCGGCGCCGGCCTGACCAAGGCCTGCGGCTCCGCCGCCTGCGCATCGGTGGTGGCCGCGGCCCGCACCAGGCGC
ACCGGCCGCAGCGTCAACCTTTTGACGCCCGGCGGCGGCACGCTGCATGTCGAATGGCTAGATAACGACCACGTCATCCT
GACCGGTGCCGCCGAGTGGGAATTTTCCGGCAGCTTCGATCCCTCGACCGGCACCTGGGCCCGCGACACCGAAAGCGCGG
CCTGA

Upstream 100 bases:

>100_bases
CAGGTCCGGCGTCTTCGCCGTGCTGAACACGCCTGCGCACTGGTAATTCCCGCCCGTTTCCGCCATATAGCGCCAAACGG
ACACGGAATTTCGCGGAGCT

Downstream 100 bases:

>100_bases
TGTCGGTTGCTCTGCCCAGGAGCCCGGACGTCGACGGCTCCCTTTCCGAGGCCCCCAACCGCATTGACGTGGTGACATTC
GGCTGCCGGCTGAACACCTA

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MASTAPFAKMNGIGNEIIVADMRGRADQVTAAAALALNGDAATRFDQIMAIHDARTPGTAYYVDILNSDGTSAQACGNGM
RCVVQALAAETGQKTFTFETRAGILNAREHADGTISVDMGMPHFGWQEIPLAEEFRDTRMIELQIGPIDAPVLHSPSAVS
MGNPHAIFWVDRDVWSYELERFGPLLENHPIFPERANITIAQVTSPESMIIRTWERGAGLTKACGSAACASVVAAARTRR
TGRSVNLLTPGGGTLHVEWLDNDHVILTGAAEWEFSGSFDPSTGTWARDTESAA

Sequences:

>Translated_294_residues
MASTAPFAKMNGIGNEIIVADMRGRADQVTAAAALALNGDAATRFDQIMAIHDARTPGTAYYVDILNSDGTSAQACGNGM
RCVVQALAAETGQKTFTFETRAGILNAREHADGTISVDMGMPHFGWQEIPLAEEFRDTRMIELQIGPIDAPVLHSPSAVS
MGNPHAIFWVDRDVWSYELERFGPLLENHPIFPERANITIAQVTSPESMIIRTWERGAGLTKACGSAACASVVAAARTRR
TGRSVNLLTPGGGTLHVEWLDNDHVILTGAAEWEFSGSFDPSTGTWARDTESAA
>Mature_293_residues
ASTAPFAKMNGIGNEIIVADMRGRADQVTAAAALALNGDAATRFDQIMAIHDARTPGTAYYVDILNSDGTSAQACGNGMR
CVVQALAAETGQKTFTFETRAGILNAREHADGTISVDMGMPHFGWQEIPLAEEFRDTRMIELQIGPIDAPVLHSPSAVSM
GNPHAIFWVDRDVWSYELERFGPLLENHPIFPERANITIAQVTSPESMIIRTWERGAGLTKACGSAACASVVAAARTRRT
GRSVNLLTPGGGTLHVEWLDNDHVILTGAAEWEFSGSFDPSTGTWARDTESAA

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=279, Percent_Identity=32.9749103942652, Blast_Score=134, Evalue=9e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_RHILO (Q98EB6)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_105218.1
- ProteinModelPortal:   Q98EB6
- SMR:   Q98EB6
- GeneID:   1227879
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr4318
- NMPDR:   fig|266835.1.peg.3322
- HOGENOM:   HBG399442
- OMA:   TRTWERG
- ProtClustDB:   PRK00450
- BRENDA:   5.1.1.7
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 31560; Mature: 31429

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 76-76 ACT_SITE 224-224

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASTAPFAKMNGIGNEIIVADMRGRADQVTAAAALALNGDAATRFDQIMAIHDARTPGTA
CCCCCCCHHHCCCCCEEEEEECCCCCHHHHEEEEEEECCCHHHHHHHEEEEECCCCCCCE
YYVDILNSDGTSAQACGNGMRCVVQALAAETGQKTFTFETRAGILNAREHADGTISVDMG
EEEEEECCCCCCHHHHCCHHHHHHHHHHHHCCCEEEEEEHHCCCCCCCCCCCCEEEEECC
MPHFGWQEIPLAEEFRDTRMIELQIGPIDAPVLHSPSAVSMGNPHAIFWVDRDVWSYELE
CCCCCCCCCCCHHHHCCCEEEEEEECCCCCCCCCCCCEECCCCCEEEEEEECCHHHHHHH
RFGPLLENHPIFPERANITIAQVTSPESMIIRTWERGAGLTKACGSAACASVVAAARTRR
HHCCHHHCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHC
TGRSVNLLTPGGGTLHVEWLDNDHVILTGAAEWEFSGSFDPSTGTWARDTESAA
CCCEEEEECCCCCEEEEEEECCCCEEEEECCCEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ASTAPFAKMNGIGNEIIVADMRGRADQVTAAAALALNGDAATRFDQIMAIHDARTPGTA
CCCCCCHHHCCCCCEEEEEECCCCCHHHHEEEEEEECCCHHHHHHHEEEEECCCCCCCE
YYVDILNSDGTSAQACGNGMRCVVQALAAETGQKTFTFETRAGILNAREHADGTISVDMG
EEEEEECCCCCCHHHHCCHHHHHHHHHHHHCCCEEEEEEHHCCCCCCCCCCCCEEEEECC
MPHFGWQEIPLAEEFRDTRMIELQIGPIDAPVLHSPSAVSMGNPHAIFWVDRDVWSYELE
CCCCCCCCCCCHHHHCCCEEEEEEECCCCCCCCCCCCEECCCCCEEEEEEECCHHHHHHH
RFGPLLENHPIFPERANITIAQVTSPESMIIRTWERGAGLTKACGSAACASVVAAARTRR
HHCCHHHCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHC
TGRSVNLLTPGGGTLHVEWLDNDHVILTGAAEWEFSGSFDPSTGTWARDTESAA
CCCEEEEECCCCCEEEEEEECCCCEEEEECCCEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968