Click here to switch to the map view.

The map label for this gene is trmD

Identifier: 13473625

GI number: 13473625

Start: 3414241

End: 3414939

Strand: Reverse

Name: trmD

Synonym: mll4287

Alternate gene names: 13473625

Gene position: 3414939-3414241 (Counterclockwise)

Preceding gene: 13473626

Following gene: 13473622

Centisome position: 48.53

GC content: 68.24

Gene sequence:

>699_bases
GTGACATTCAAAGCCTCGGTGCTGACGCTCTACCCCGAGATGTTTCCGGGCGCGCTCGGCCTGTCGCTGGCGGGCCGCGC
GCTCGAGGCCGGCACCTGGTCGCTGGAAGCGATCCAGATCCGCGATTTCGCCACCGACAAGCACCGCACCGTCGACGACA
CGCCGGCCGGCGGCGGCGCCGGCATGGTGATGCGTGCCGATGTGCTGGCCAGGGCAATCGACCATGCCTCGCCGCCGGGC
GATACGCGTCCGCGCCTTTTGATGAGCCCACGCGGCAAACCGCTGACCCAGGCGCGGGTGCGGGAACTTGCTGCCGGGCC
GGGTGCCGTCATCCTGTGCGGCCGCTTCGAGGGCGTCGACCAGCGGCTGATCGAGGTGCGGGGATTAGAGGAGGTGTCGA
TCGGCGATTTCATCCTCTCCGGCGGCGAGCCGGCGGCGCTCGTGCTGCTCGACGCGGTGGTGCGGCTGCTGCCCGGCGTG
ATGGGCAATGCGGTATCCGGCGAGGAAGAGAGTTTCGAGAACGGCCTGCTTGAACATCCGCACTACACAAGGCCGCAGGA
GTTCGAGGGCCGGGAGATTCCGCAAGTGCTGACCTCGGGCAATCACAAGAAGATCGCCGCCTGGCGGCGGGAGCAGGCGG
AGCAGCTGACCAGGGAGAGGCGGCCGGATCTTCTCGGCGCTCACCCCTTGGCAAAATAG

Upstream 100 bases:

>100_bases
CGGCCGGGCTGGTCGAGGACGAGGACGGCGATGCGCCGCGCGAGGAAGACTTCGACCCGAAGGGCAGGCCGCGCGGACCG
CGCGACGCCGGGGGCAACCG

Downstream 100 bases:

>100_bases
TAAAACGCCAGAAACAGGCCGACGGCGACGACGAAGCCGCGCACCACGTTCTGCGGCACGCGCTTGGCGATCCAGACGCC
GGCATAGCCGCCGAGCGCAC

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MTFKASVLTLYPEMFPGALGLSLAGRALEAGTWSLEAIQIRDFATDKHRTVDDTPAGGGAGMVMRADVLARAIDHASPPG
DTRPRLLMSPRGKPLTQARVRELAAGPGAVILCGRFEGVDQRLIEVRGLEEVSIGDFILSGGEPAALVLLDAVVRLLPGV
MGNAVSGEEESFENGLLEHPHYTRPQEFEGREIPQVLTSGNHKKIAAWRREQAEQLTRERRPDLLGAHPLAK

Sequences:

>Translated_232_residues
MTFKASVLTLYPEMFPGALGLSLAGRALEAGTWSLEAIQIRDFATDKHRTVDDTPAGGGAGMVMRADVLARAIDHASPPG
DTRPRLLMSPRGKPLTQARVRELAAGPGAVILCGRFEGVDQRLIEVRGLEEVSIGDFILSGGEPAALVLLDAVVRLLPGV
MGNAVSGEEESFENGLLEHPHYTRPQEFEGREIPQVLTSGNHKKIAAWRREQAEQLTRERRPDLLGAHPLAK
>Mature_231_residues
TFKASVLTLYPEMFPGALGLSLAGRALEAGTWSLEAIQIRDFATDKHRTVDDTPAGGGAGMVMRADVLARAIDHASPPGD
TRPRLLMSPRGKPLTQARVRELAAGPGAVILCGRFEGVDQRLIEVRGLEEVSIGDFILSGGEPAALVLLDAVVRLLPGVM
GNAVSGEEESFENGLLEHPHYTRPQEFEGREIPQVLTSGNHKKIAAWRREQAEQLTRERRPDLLGAHPLAK

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=220, Percent_Identity=46.3636363636364, Blast_Score=194, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_RHILO (Q98EE1)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_105193.1
- ProteinModelPortal:   Q98EE1
- SMR:   Q98EE1
- GeneID:   1227854
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll4287
- NMPDR:   fig|266835.1.peg.3297
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- BRENDA:   2.1.1.31
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 25082; Mature: 24951

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: BINDING 114-114

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFKASVLTLYPEMFPGALGLSLAGRALEAGTWSLEAIQIRDFATDKHRTVDDTPAGGGA
CCCCCHHHHHHHHHCCCHHHHHHHCCHHCCCCCEEEEEEEEHHCCCCCCCCCCCCCCCCC
GMVMRADVLARAIDHASPPGDTRPRLLMSPRGKPLTQARVRELAAGPGAVILCGRFEGVD
CHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCHH
QRLIEVRGLEEVSIGDFILSGGEPAALVLLDAVVRLLPGVMGNAVSGEEESFENGLLEHP
HHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCC
HYTRPQEFEGREIPQVLTSGNHKKIAAWRREQAEQLTRERRPDLLGAHPLAK
CCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TFKASVLTLYPEMFPGALGLSLAGRALEAGTWSLEAIQIRDFATDKHRTVDDTPAGGGA
CCCCHHHHHHHHHCCCHHHHHHHCCHHCCCCCEEEEEEEEHHCCCCCCCCCCCCCCCCC
GMVMRADVLARAIDHASPPGDTRPRLLMSPRGKPLTQARVRELAAGPGAVILCGRFEGVD
CHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCHH
QRLIEVRGLEEVSIGDFILSGGEPAALVLLDAVVRLLPGVMGNAVSGEEESFENGLLEHP
HHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCC
HYTRPQEFEGREIPQVLTSGNHKKIAAWRREQAEQLTRERRPDLLGAHPLAK
CCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968