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The map label for this gene is leuC

Identifier: 13473614

GI number: 13473614

Start: 3406055

End: 3407464

Strand: Reverse

Name: leuC

Synonym: mll4272

Alternate gene names: 13473614

Gene position: 3407464-3406055 (Counterclockwise)

Preceding gene: 13473615

Following gene: 13473613

Centisome position: 48.43

GC content: 64.61

Gene sequence:

>1410_bases
ATGAGCGCACCGCGCACCCTCTACGACAAGATTTTCGACGACCACGTCGTCGACCGCCAGGACGATGGCACCTGCCTGCT
CTACATCGACCGCCACCTCGTCCATGAAGTGACCAGCCCGCAGGCCTTCGAAGGCCTGCGCCTGAGCGGCCGTAAGGTCC
GCCATCCGGAAAAGACGCTGGCCGTGGTCGATCACAATGTCTCGACCTCGCCCGAGCGCAAGTTCGGCATCAAGAACGAG
GAAAGCCGCATCCAGGTCGAGGCGCTGGCCAAGAACGCCAAGGACTTCGGCGTCGAATATTATTCCGAGAACGATATCCG
CCAGGGCATCGTCCACATCATCGGCCCGGAGCAGGGCTTTACCTTGCCCGGCATGACCATCGTCTGCGGCGACAGCCACA
CGTCGACGCATGGCGCGTTCGGCGCGCTGGCGCACGGCATCGGCACCTCGGAAGTCGAGCATGTGCTGGCGACGCAGACG
CTGATCCAGCGCAAGGCCAAGAACATGCTGGTGCGTGTCGACGGCCAGCTGCCGGAAGGCGTCACCGCCAAGGACATCAT
CCTGGCCATCATTGGCGAGATTGGCACCGCCGGCGGCACCGGCTATGTCATCGAATATGCCGGCGAGGCGATCCGCTCGC
TGTCGATGGAAGGCCGCATGACGATCTGCAACATGTCGATCGAGGGCGGTGCGCGCGCCGGCCTGATCGCCGCCGACGAG
ACCACCTTCGCCTATGTCAAGGACAAGCCGCGCGCGCCGAAGGGCGCGGCCTGGGATGCCGCGCTCGCCTACTGGAAGAC
GCTGCAGTCCGACGAAGGCGCGCATTTCGACAAGGTGATCGTGCTCGACGCGGCCAAGCTGCCGCCGATCGTGTCCTGGG
GCTCCTCGCCCGAGGATGTGGTTTCGGTGCAGGGCGTCGTTCCCAATCCGGAAGAGATCACGGACGAGAACAAACGCACC
TCAAAGATCCGCGCGCTCGACTATATGGGCCTGACGCCGGGCACCAAGATCACCGATATCGCGCTCGACCGCGTCTTCAT
CGGCTCCTGCACCAATGGCCGCATCGAGGATCTGCGCGCCGCCGCCAAGGTGATCGAGGGCAAGACCGTCAATCCGCGCG
TCAACGCCATGATCGTGCCGGGCTCCGGCCTGGTCAAGGAACAGGCGGAAGCCGAGGGTCTGGACAAGATCTTCCTCGCC
GCCGGGTTCGACTGGCGCGAGCCGGGCTGCTCGATGTGCCTGGCCATGAACGACGACAGGCTGAAGCCGCATGAACGCTG
TGCCTCGACCTCGAACCGCAATTTCGAGGGAAGGCAAGGCTTCAAGGGCCGCACCCATCTGGTGTCGCCGGCCATGGCGG
CGGCGGCGGCGATCGCCGGCCACTTCGTCGACATCCGTGATTGGAAGTAA

Upstream 100 bases:

>100_bases
TGCGGCGCTTTGACCAGCCGACTTAGCTAGGCCGCTTTCGCCGGGCCTAGGGCTTGCGGCGTTACACTCTTCCCATTCTC
ATATCGACGGATTTACGCAC

Downstream 100 bases:

>100_bases
CCTCCGGTCCACACGTTCCGATGACTGCCGGACCCCGGACCGCATTGCGGTTCCGGGGTCTTTTTCATTCACGCGGTCCG
CATATTCGGCCGCGCTTAAC

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSAPRTLYDKIFDDHVVDRQDDGTCLLYIDRHLVHEVTSPQAFEGLRLSGRKVRHPEKTLAVVDHNVSTSPERKFGIKNE
ESRIQVEALAKNAKDFGVEYYSENDIRQGIVHIIGPEQGFTLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQT
LIQRKAKNMLVRVDGQLPEGVTAKDIILAIIGEIGTAGGTGYVIEYAGEAIRSLSMEGRMTICNMSIEGGARAGLIAADE
TTFAYVKDKPRAPKGAAWDAALAYWKTLQSDEGAHFDKVIVLDAAKLPPIVSWGSSPEDVVSVQGVVPNPEEITDENKRT
SKIRALDYMGLTPGTKITDIALDRVFIGSCTNGRIEDLRAAAKVIEGKTVNPRVNAMIVPGSGLVKEQAEAEGLDKIFLA
AGFDWREPGCSMCLAMNDDRLKPHERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDIRDWK

Sequences:

>Translated_469_residues
MSAPRTLYDKIFDDHVVDRQDDGTCLLYIDRHLVHEVTSPQAFEGLRLSGRKVRHPEKTLAVVDHNVSTSPERKFGIKNE
ESRIQVEALAKNAKDFGVEYYSENDIRQGIVHIIGPEQGFTLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQT
LIQRKAKNMLVRVDGQLPEGVTAKDIILAIIGEIGTAGGTGYVIEYAGEAIRSLSMEGRMTICNMSIEGGARAGLIAADE
TTFAYVKDKPRAPKGAAWDAALAYWKTLQSDEGAHFDKVIVLDAAKLPPIVSWGSSPEDVVSVQGVVPNPEEITDENKRT
SKIRALDYMGLTPGTKITDIALDRVFIGSCTNGRIEDLRAAAKVIEGKTVNPRVNAMIVPGSGLVKEQAEAEGLDKIFLA
AGFDWREPGCSMCLAMNDDRLKPHERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDIRDWK
>Mature_468_residues
SAPRTLYDKIFDDHVVDRQDDGTCLLYIDRHLVHEVTSPQAFEGLRLSGRKVRHPEKTLAVVDHNVSTSPERKFGIKNEE
SRIQVEALAKNAKDFGVEYYSENDIRQGIVHIIGPEQGFTLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTL
IQRKAKNMLVRVDGQLPEGVTAKDIILAIIGEIGTAGGTGYVIEYAGEAIRSLSMEGRMTICNMSIEGGARAGLIAADET
TFAYVKDKPRAPKGAAWDAALAYWKTLQSDEGAHFDKVIVLDAAKLPPIVSWGSSPEDVVSVQGVVPNPEEITDENKRTS
KIRALDYMGLTPGTKITDIALDRVFIGSCTNGRIEDLRAAAKVIEGKTVNPRVNAMIVPGSGLVKEQAEAEGLDKIFLAA
GFDWREPGCSMCLAMNDDRLKPHERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDIRDWK

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI8659555, Length=454, Percent_Identity=24.6696035242291, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI4501867, Length=380, Percent_Identity=27.6315789473684, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI41352693, Length=367, Percent_Identity=25.3405994550409, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1786259, Length=465, Percent_Identity=66.2365591397849, Blast_Score=624, Evalue=1e-180,
Organism=Escherichia coli, GI1787531, Length=359, Percent_Identity=27.0194986072423, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87081781, Length=340, Percent_Identity=24.4117647058824, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25149337, Length=382, Percent_Identity=28.7958115183246, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI32564738, Length=382, Percent_Identity=28.7958115183246, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI25149342, Length=300, Percent_Identity=28.6666666666667, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17568399, Length=378, Percent_Identity=26.1904761904762, Blast_Score=98, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321429, Length=472, Percent_Identity=64.8305084745763, Blast_Score=620, Evalue=1e-178,
Organism=Saccharomyces cerevisiae, GI6320440, Length=427, Percent_Identity=26.9320843091335, Blast_Score=154, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6322261, Length=383, Percent_Identity=29.7650130548303, Blast_Score=132, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6323335, Length=361, Percent_Identity=29.0858725761773, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI281365315, Length=483, Percent_Identity=27.1221532091097, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI17864292, Length=483, Percent_Identity=27.1221532091097, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI161076999, Length=382, Percent_Identity=27.7486910994764, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI28571643, Length=362, Percent_Identity=27.6243093922652, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24645686, Length=381, Percent_Identity=27.8215223097113, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI17137564, Length=381, Percent_Identity=27.0341207349081, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_RHILO (Q98EF1)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_105182.1
- ProteinModelPortal:   Q98EF1
- SMR:   Q98EF1
- GeneID:   1227843
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll4272
- NMPDR:   fig|266835.1.peg.3286
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- ProtClustDB:   PRK05478
- BRENDA:   4.2.1.33
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 50872; Mature: 50741

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPRTLYDKIFDDHVVDRQDDGTCLLYIDRHLVHEVTSPQAFEGLRLSGRKVRHPEKTL
CCCCHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHCCCCHHHCCCCCCCCCCCCCCCEE
AVVDHNVSTSPERKFGIKNEESRIQVEALAKNAKDFGVEYYSENDIRQGIVHIIGPEQGF
EEEECCCCCCCCHHCCCCCCCHHEEHHHHHCCHHHCCEEEECCCCHHCCEEEEECCCCCC
TLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLIQRKAKNMLVRVDGQLPEG
CCCCEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCC
VTAKDIILAIIGEIGTAGGTGYVIEYAGEAIRSLSMEGRMTICNMSIEGGARAGLIAADE
CCHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEECC
TTFAYVKDKPRAPKGAAWDAALAYWKTLQSDEGAHFDKVIVLDAAKLPPIVSWGSSPEDV
CEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEECCCCHHHE
VSVQGVVPNPEEITDENKRTSKIRALDYMGLTPGTKITDIALDRVFIGSCTNGRIEDLRA
EEEECCCCCCHHHCCCCHHHHHEEEEEEECCCCCCEEHHEEECEEEEECCCCCHHHHHHH
AAKVIEGKTVNPRVNAMIVPGSGLVKEQAEAEGLDKIFLAAGFDWREPGCSMCLAMNDDR
HHHHHCCCCCCCCCCEEEECCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCC
LKPHERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDIRDWK
CCHHHHHHCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHEEEEECCC
>Mature Secondary Structure 
SAPRTLYDKIFDDHVVDRQDDGTCLLYIDRHLVHEVTSPQAFEGLRLSGRKVRHPEKTL
CCCHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHCCCCHHHCCCCCCCCCCCCCCCEE
AVVDHNVSTSPERKFGIKNEESRIQVEALAKNAKDFGVEYYSENDIRQGIVHIIGPEQGF
EEEECCCCCCCCHHCCCCCCCHHEEHHHHHCCHHHCCEEEECCCCHHCCEEEEECCCCCC
TLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLIQRKAKNMLVRVDGQLPEG
CCCCEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCC
VTAKDIILAIIGEIGTAGGTGYVIEYAGEAIRSLSMEGRMTICNMSIEGGARAGLIAADE
CCHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEEECC
TTFAYVKDKPRAPKGAAWDAALAYWKTLQSDEGAHFDKVIVLDAAKLPPIVSWGSSPEDV
CEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEECCCCHHHE
VSVQGVVPNPEEITDENKRTSKIRALDYMGLTPGTKITDIALDRVFIGSCTNGRIEDLRA
EEEECCCCCCHHHCCCCHHHHHEEEEEEECCCCCCEEHHEEECEEEEECCCCCHHHHHHH
AAKVIEGKTVNPRVNAMIVPGSGLVKEQAEAEGLDKIFLAAGFDWREPGCSMCLAMNDDR
HHHHHCCCCCCCCCCEEEECCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCC
LKPHERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDIRDWK
CCHHHHHHCCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968