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The map label for this gene is hemC

Identifier: 13473577

GI number: 13473577

Start: 3373336

End: 3374262

Strand: Reverse

Name: hemC

Synonym: mll4223

Alternate gene names: 13473577

Gene position: 3374262-3373336 (Counterclockwise)

Preceding gene: 13473584

Following gene: 13473576

Centisome position: 47.96

GC content: 67.64

Gene sequence:

>927_bases
ATGCAAACAACCTTGAAAATCGGAACACGGGGCAGCCCGCTGGCGCTGGCCCAGGCGCATGAAACGCAGGCGCGGCTGAT
GGCCGCGCATGGCCTGCCGGCGGAAGCCTTCGAGGTCGTCGTCATCTCGACCAGCGGCGACCGCATCCAGGACCGGCCGC
TGTCGGAGGCCGGCGGCAAGGGCCTGTTCACCAAGGAAATCGAGGAAGCGCTGCTCGCCGGTGCGATCGACATCGCCGTG
CATTCGTCCAAGGACATGCCGACGCAATTGCCCGATGGCCTGGAGCTTTCCGCCTTCCTGCCGCGCGAGGACGCGCGCGA
CGCCTTTGTCGGCAAGGCGGCAAAGACCATCGCCGACCTGCTGCGCGGGGCCAAGGTCGGCTCGTCGTCGCTCAGGCGGC
AGGCACTGATCCGCCGCATGCGGCCGGATCTCGACGTGGTGATGTTCCGCGGCAATGTGCAGACGCGGCTGCGCAAGCTC
GACGAAGGGGTCGCCGCCGGCACCATCCTCGCCTATGCCGGGCTGAAGCGGCTGGGTCTGGAGCATGTCGCCACCGACTT
GATGCCGCTCGACATTTTTCCGCCCGCACCCGGCCAGGGGGCGATCGGCATCGAGACGCGCATCGGTGATCGCGCCGTCG
AAAAGATGCTGGTCGCCATCCACGATGTGCCGACGGGGCAGGCGCTGGCCTGCGAGCGCGCCTTCCTGGCGGCGCTCGAC
GGCTCCTGCCGCACGCCGATCGCCGGCCATGCGACTATCGAGACCGGAAATCTCTCTTTCGCCGGCCTGATCATCTCACC
CGACGGGACGCAGTCGCATACGGTCGAATTGCAGGGACCAGCACAGGATGCCGCCCGCATCGGCGATGAGGCCGCACGGA
CGGTGCGGGCCAAGGCCGGGGAAAAATTCTTCGACGGCTGGGTCTGA

Upstream 100 bases:

>100_bases
CCCGGAAAGGCCTCGGTTATCTTAGCCGCATTTCTGCGACGCCAAGTGGCTCCACTTGGTCGCAAATGCTCCGGCCGGGG
GTTATCACGGACGGCGCGCC

Downstream 100 bases:

>100_bases
CGATGCTTCGCGTCCTGGTGACCAGGCCGGAACCGGGCGCCTCTCGCACAGCGCTGCGGCTTCAGGAGACGGGCTTTCAA
CCGGTCGTTCTGCCTTTGAC

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MQTTLKIGTRGSPLALAQAHETQARLMAAHGLPAEAFEVVVISTSGDRIQDRPLSEAGGKGLFTKEIEEALLAGAIDIAV
HSSKDMPTQLPDGLELSAFLPREDARDAFVGKAAKTIADLLRGAKVGSSSLRRQALIRRMRPDLDVVMFRGNVQTRLRKL
DEGVAAGTILAYAGLKRLGLEHVATDLMPLDIFPPAPGQGAIGIETRIGDRAVEKMLVAIHDVPTGQALACERAFLAALD
GSCRTPIAGHATIETGNLSFAGLIISPDGTQSHTVELQGPAQDAARIGDEAARTVRAKAGEKFFDGWV

Sequences:

>Translated_308_residues
MQTTLKIGTRGSPLALAQAHETQARLMAAHGLPAEAFEVVVISTSGDRIQDRPLSEAGGKGLFTKEIEEALLAGAIDIAV
HSSKDMPTQLPDGLELSAFLPREDARDAFVGKAAKTIADLLRGAKVGSSSLRRQALIRRMRPDLDVVMFRGNVQTRLRKL
DEGVAAGTILAYAGLKRLGLEHVATDLMPLDIFPPAPGQGAIGIETRIGDRAVEKMLVAIHDVPTGQALACERAFLAALD
GSCRTPIAGHATIETGNLSFAGLIISPDGTQSHTVELQGPAQDAARIGDEAARTVRAKAGEKFFDGWV
>Mature_308_residues
MQTTLKIGTRGSPLALAQAHETQARLMAAHGLPAEAFEVVVISTSGDRIQDRPLSEAGGKGLFTKEIEEALLAGAIDIAV
HSSKDMPTQLPDGLELSAFLPREDARDAFVGKAAKTIADLLRGAKVGSSSLRRQALIRRMRPDLDVVMFRGNVQTRLRKL
DEGVAAGTILAYAGLKRLGLEHVATDLMPLDIFPPAPGQGAIGIETRIGDRAVEKMLVAIHDVPTGQALACERAFLAALD
GSCRTPIAGHATIETGNLSFAGLIISPDGTQSHTVELQGPAQDAARIGDEAARTVRAKAGEKFFDGWV

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI66933009, Length=290, Percent_Identity=40, Blast_Score=185, Evalue=5e-47,
Organism=Homo sapiens, GI20149500, Length=289, Percent_Identity=40.1384083044983, Blast_Score=185, Evalue=5e-47,
Organism=Escherichia coli, GI48994974, Length=288, Percent_Identity=46.1805555555556, Blast_Score=231, Evalue=4e-62,
Organism=Saccharomyces cerevisiae, GI6319996, Length=317, Percent_Identity=36.9085173501577, Blast_Score=171, Evalue=1e-43,
Organism=Drosophila melanogaster, GI20130425, Length=283, Percent_Identity=41.3427561837456, Blast_Score=184, Evalue=7e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_RHILO (Q98EI7)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_105145.1
- ProteinModelPortal:   Q98EI7
- SMR:   Q98EI7
- GeneID:   1227806
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll4223
- NMPDR:   fig|266835.1.peg.3249
- HOGENOM:   HBG624842
- OMA:   SLHAVGQ
- ProtClustDB:   PRK00072
- BRENDA:   2.5.1.61
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 32580; Mature: 32580

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTTLKIGTRGSPLALAQAHETQARLMAAHGLPAEAFEVVVISTSGDRIQDRPLSEAGGK
CCCEEEECCCCCCEEEHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCHHCCCC
GLFTKEIEEALLAGAIDIAVHSSKDMPTQLPDGLELSAFLPREDARDAFVGKAAKTIADL
CCHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHH
LRGAKVGSSSLRRQALIRRMRPDLDVVMFRGNVQTRLRKLDEGVAAGTILAYAGLKRLGL
HHCCCCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
EHVATDLMPLDIFPPAPGQGAIGIETRIGDRAVEKMLVAIHDVPTGQALACERAFLAALD
HHHHHHCEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
GSCRTPIAGHATIETGNLSFAGLIISPDGTQSHTVELQGPAQDAARIGDEAARTVRAKAG
CCCCCCCCCCEEEEECCEEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCC
EKFFDGWV
CHHHCCCC
>Mature Secondary Structure
MQTTLKIGTRGSPLALAQAHETQARLMAAHGLPAEAFEVVVISTSGDRIQDRPLSEAGGK
CCCEEEECCCCCCEEEHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCHHCCCC
GLFTKEIEEALLAGAIDIAVHSSKDMPTQLPDGLELSAFLPREDARDAFVGKAAKTIADL
CCHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHH
LRGAKVGSSSLRRQALIRRMRPDLDVVMFRGNVQTRLRKLDEGVAAGTILAYAGLKRLGL
HHCCCCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
EHVATDLMPLDIFPPAPGQGAIGIETRIGDRAVEKMLVAIHDVPTGQALACERAFLAALD
HHHHHHCEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
GSCRTPIAGHATIETGNLSFAGLIISPDGTQSHTVELQGPAQDAARIGDEAARTVRAKAG
CCCCCCCCCCEEEEECCEEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCC
EKFFDGWV
CHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968