Click here to switch to the map view.

The map label for this gene is pcaF [H]

Identifier: 13473539

GI number: 13473539

Start: 3334462

End: 3335667

Strand: Reverse

Name: pcaF [H]

Synonym: mll4180

Alternate gene names: 13473539

Gene position: 3335667-3334462 (Counterclockwise)

Preceding gene: 13473540

Following gene: 13473538

Centisome position: 47.41

GC content: 67.08

Gene sequence:

>1206_bases
ATGGCCGAGGCCTATATCTGCGATTACATCCGCACGCCGATCGGCCGCTTCGGCGGGTCGCTGTCCTCGGTCCGATCAGA
CGACCTCGGCGCCATTCCGCTGAAGGCTTTGGCCGAGCGCAATGCCGGTATCGACTGGCAGGCGGTGGACGACGTCGTCT
ATGGCTGCGCCAACCAGGCCGGCGAGGACAACCGCAACGTGGCGCGCATGGCGCTGCTCCTGGCTGGCCTGCCGAAGGAA
ATCCCGGGTTCGACCGTCAATCGCCTGTGCGGGTCCGGCATGGATGCGTTGACCATCGCGGCGCGCGCCATCAAGGCCGG
CGAGGCGGAATTGATGATTGCCGGTGGCGTCGAATCGATGAGCCGGGCGCCCTTCGTCATGCCCAAGGCCGACACGGCGT
TTTCGCGCAATGCCGAGATTTACGACACCACGATCGGCTGGCGCTTCGTCAACCCGCTGATGAAGAAGCAATATGGCGTC
GATTCCATGCCGGAAACCGGCGAGAACGTCGCCGAGGATTTTGCCGTGTCTCGCGCCGACCAGGACGCCTTTGCCGTGCG
CAGCCAGGACAAGGCTGTCGCCGCGCAGGCCAATGGCAGGCTGGCCAAGGAGATCACAGCGGTGACGATCCCGCAGCGCA
AGGGCGATCCGGTGGTGGTCTCGAAAGACGAACATCCACGCGCCGGCACCACGGTCGAGGCGCTGGCCAAGCTGCCGACG
CCGTTCCGCCAGGGCGGCACGGTGACCGCCGGCAATGCCTCCGGCGTCAATGACGGGGCGGCGGTGCTGATCGTTGCTTC
CGAGGCTGCGGTGAAAAAATATGGCCTGACGCCGATTGCCCGCATCCTCGGTGGTGCAGCCGCCGGTGTGGCGCCGCGCA
TCATGGGTATCGGCCCGGCGCCGGCGACGCAGAAACTGTGCGCGCGGCTCGGCCTGACGCCGCAACAGTTCGATGTCATC
GAGCTCAACGAAGCCTTTGCCTCGCAAGGCATCGCCGTGCTGCGCCAGCTCGGCATTGCCGAGGATGCCGAGCACATCAA
CCCGAATGGCGGCGCCATCGCGCTCGGCCATCCGCTGGGGATGTCGGGTGCGCGCATCTCCGGCACGGCGGCGCTGGAGC
TGCGTGAGCGCGGCGGTCGCTACGCGCTTGCGACGATGTGCATCGGCGTCGGCCAGGGCATCGCCATCGCGCTCGAACGG
GCCTGA

Upstream 100 bases:

>100_bases
ACACCGGCGCCAAGCGAACTCGAACTCAACACATTGCGCGACCTGCAGGCCCGCACCAAGGCGGCGCATGAGGGAACGGG
AAACAAGAAAGCTGCATGAC

Downstream 100 bases:

>100_bases
CCGTCGCCTTCCCTTCGCCAGCGAGAATCGGGGCTGGATTCGGCGCGCCGTCAAATTTGACCATGTGGACAAAAGTCCGG
ACCCGTTCCATAGTTCCCCG

Product: beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MAEAYICDYIRTPIGRFGGSLSSVRSDDLGAIPLKALAERNAGIDWQAVDDVVYGCANQAGEDNRNVARMALLLAGLPKE
IPGSTVNRLCGSGMDALTIAARAIKAGEAELMIAGGVESMSRAPFVMPKADTAFSRNAEIYDTTIGWRFVNPLMKKQYGV
DSMPETGENVAEDFAVSRADQDAFAVRSQDKAVAAQANGRLAKEITAVTIPQRKGDPVVVSKDEHPRAGTTVEALAKLPT
PFRQGGTVTAGNASGVNDGAAVLIVASEAAVKKYGLTPIARILGGAAAGVAPRIMGIGPAPATQKLCARLGLTPQQFDVI
ELNEAFASQGIAVLRQLGIAEDAEHINPNGGAIALGHPLGMSGARISGTAALELRERGGRYALATMCIGVGQGIAIALER
A

Sequences:

>Translated_401_residues
MAEAYICDYIRTPIGRFGGSLSSVRSDDLGAIPLKALAERNAGIDWQAVDDVVYGCANQAGEDNRNVARMALLLAGLPKE
IPGSTVNRLCGSGMDALTIAARAIKAGEAELMIAGGVESMSRAPFVMPKADTAFSRNAEIYDTTIGWRFVNPLMKKQYGV
DSMPETGENVAEDFAVSRADQDAFAVRSQDKAVAAQANGRLAKEITAVTIPQRKGDPVVVSKDEHPRAGTTVEALAKLPT
PFRQGGTVTAGNASGVNDGAAVLIVASEAAVKKYGLTPIARILGGAAAGVAPRIMGIGPAPATQKLCARLGLTPQQFDVI
ELNEAFASQGIAVLRQLGIAEDAEHINPNGGAIALGHPLGMSGARISGTAALELRERGGRYALATMCIGVGQGIAIALER
A
>Mature_400_residues
AEAYICDYIRTPIGRFGGSLSSVRSDDLGAIPLKALAERNAGIDWQAVDDVVYGCANQAGEDNRNVARMALLLAGLPKEI
PGSTVNRLCGSGMDALTIAARAIKAGEAELMIAGGVESMSRAPFVMPKADTAFSRNAEIYDTTIGWRFVNPLMKKQYGVD
SMPETGENVAEDFAVSRADQDAFAVRSQDKAVAAQANGRLAKEITAVTIPQRKGDPVVVSKDEHPRAGTTVEALAKLPTP
FRQGGTVTAGNASGVNDGAAVLIVASEAAVKKYGLTPIARILGGAAAGVAPRIMGIGPAPATQKLCARLGLTPQQFDVIE
LNEAFASQGIAVLRQLGIAEDAEHINPNGGAIALGHPLGMSGARISGTAALELRERGGRYALATMCIGVGQGIAIALERA

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=389, Percent_Identity=42.159383033419, Blast_Score=281, Evalue=7e-76,
Organism=Homo sapiens, GI148539872, Length=392, Percent_Identity=38.7755102040816, Blast_Score=253, Evalue=3e-67,
Organism=Homo sapiens, GI4557237, Length=401, Percent_Identity=36.6583541147132, Blast_Score=230, Evalue=1e-60,
Organism=Homo sapiens, GI4501853, Length=398, Percent_Identity=38.4422110552764, Blast_Score=212, Evalue=6e-55,
Organism=Homo sapiens, GI4504327, Length=425, Percent_Identity=30.5882352941176, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI194353979, Length=391, Percent_Identity=29.156010230179, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1787663, Length=400, Percent_Identity=65.5, Blast_Score=536, Evalue=1e-154,
Organism=Escherichia coli, GI1788554, Length=402, Percent_Identity=47.0149253731343, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=42.643391521197, Blast_Score=281, Evalue=4e-77,
Organism=Escherichia coli, GI48994986, Length=405, Percent_Identity=43.2098765432099, Blast_Score=266, Evalue=1e-72,
Organism=Escherichia coli, GI1788683, Length=424, Percent_Identity=30.4245283018868, Blast_Score=147, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI133906874, Length=398, Percent_Identity=43.4673366834171, Blast_Score=289, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI25147385, Length=404, Percent_Identity=33.4158415841584, Blast_Score=217, Evalue=9e-57,
Organism=Caenorhabditis elegans, GI17535921, Length=405, Percent_Identity=33.0864197530864, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17551802, Length=432, Percent_Identity=29.8611111111111, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17535917, Length=402, Percent_Identity=29.6019900497512, Blast_Score=152, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6325229, Length=403, Percent_Identity=38.2133995037221, Blast_Score=216, Evalue=5e-57,
Organism=Saccharomyces cerevisiae, GI6322031, Length=339, Percent_Identity=37.1681415929204, Blast_Score=193, Evalue=5e-50,
Organism=Drosophila melanogaster, GI24655093, Length=400, Percent_Identity=43, Blast_Score=315, Evalue=3e-86,
Organism=Drosophila melanogaster, GI17648125, Length=401, Percent_Identity=40.6483790523691, Blast_Score=261, Evalue=7e-70,
Organism=Drosophila melanogaster, GI24640423, Length=405, Percent_Identity=34.5679012345679, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI17137578, Length=424, Percent_Identity=30.8962264150943, Blast_Score=152, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 41603; Mature: 41472

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEAYICDYIRTPIGRFGGSLSSVRSDDLGAIPLKALAERNAGIDWQAVDDVVYGCANQA
CCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCC
GEDNRNVARMALLLAGLPKEIPGSTVNRLCGSGMDALTIAARAIKAGEAELMIAGGVESM
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCHHHH
SRAPFVMPKADTAFSRNAEIYDTTIGWRFVNPLMKKQYGVDSMPETGENVAEDFAVSRAD
CCCCEECCCCCCHHCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCC
QDAFAVRSQDKAVAAQANGRLAKEITAVTIPQRKGDPVVVSKDEHPRAGTTVEALAKLPT
CHHHHHCCCCCEEEHHCCCCHHHHEEEEECCCCCCCCEEEECCCCCCCCCHHHHHHHCCC
PFRQGGTVTAGNASGVNDGAAVLIVASEAAVKKYGLTPIARILGGAAAGVAPRIMGIGPA
CHHCCCEEEECCCCCCCCCEEEEEEECHHHHHHHCCCHHHHHHCCHHHCCCCCEEECCCC
PATQKLCARLGLTPQQFDVIELNEAFASQGIAVLRQLGIAEDAEHINPNGGAIALGHPLG
CHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCEEEECCCCC
MSGARISGTAALELRERGGRYALATMCIGVGQGIAIALERA
CCCCEECCCEEEEEHHCCCCEEEHHHHHHCCCCEEEEEECC
>Mature Secondary Structure 
AEAYICDYIRTPIGRFGGSLSSVRSDDLGAIPLKALAERNAGIDWQAVDDVVYGCANQA
CCHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCC
GEDNRNVARMALLLAGLPKEIPGSTVNRLCGSGMDALTIAARAIKAGEAELMIAGGVESM
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCHHHH
SRAPFVMPKADTAFSRNAEIYDTTIGWRFVNPLMKKQYGVDSMPETGENVAEDFAVSRAD
CCCCEECCCCCCHHCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCC
QDAFAVRSQDKAVAAQANGRLAKEITAVTIPQRKGDPVVVSKDEHPRAGTTVEALAKLPT
CHHHHHCCCCCEEEHHCCCCHHHHEEEEECCCCCCCCEEEECCCCCCCCCHHHHHHHCCC
PFRQGGTVTAGNASGVNDGAAVLIVASEAAVKKYGLTPIARILGGAAAGVAPRIMGIGPA
CHHCCCEEEECCCCCCCCCEEEEEEECHHHHHHHCCCHHHHHHCCHHHCCCCCEEECCCC
PATQKLCARLGLTPQQFDVIELNEAFASQGIAVLRQLGIAEDAEHINPNGGAIALGHPLG
CHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCEEEECCCCC
MSGARISGTAALELRERGGRYALATMCIGVGQGIAIALERA
CCCCEECCCEEEEEHHCCCCEEEHHHHHHCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]