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The map label for this gene is rutB [H]

Identifier: 13473531

GI number: 13473531

Start: 3326307

End: 3326978

Strand: Direct

Name: rutB [H]

Synonym: mlr4169

Alternate gene names: 13473531

Gene position: 3326307-3326978 (Clockwise)

Preceding gene: 13473526

Following gene: 13473534

Centisome position: 47.28

GC content: 63.24

Gene sequence:

>672_bases
ATGATCAAGGCAACGCCCTTCGACTTCCCCTATGACGGCAGGCTGGTGGCCGAAAACACGGCACTGATCGTCATCGACCT
GCAGCAGGATTTCCTGTCGACCACGGGCTATTTCGCCAGAAAGGGATATGACCCCTCACCGCTGCGGGCGATCCTGCCCA
CGGTGAACTGGCTGATCGCCGCTTCGCGCAAAGCCGGCGTCAGGGTCATCCATACCAGGCAGGGCTATCGCGCCGACATG
GCCGACATGACGCCTTACGAGAAGTGGCGCCGCAAGCGCTCCGGCCTCGACGGCACCGATGTCCTGCTGCGCTCGGGCGC
GGGGTTCCAGATCGTTCCGGAGATCGATGTCGCGCCGCAGGACATCATCGTCGACAAGACCTGCAACAGCGCCTTCACCT
ATACGGATTTCGAGCTTGTGCTGCGCGCGCAAGGCATCACGCACCTGATGTTTTCCGGATGCACGACCGATGTCTGCGTC
CACACTACGCTGCGCGAGGCCTGCGACCGCAATTTTCAGTGCCTGACGATCTCGGACGCCTGCGCCAGTGGCGACCAGAA
AGCTCACGAGGCGGCCCTGCACATGGTGACGGTCGAGGATGGCGTCTTCGGCGTCCTGGCCGATTCAGCCGCCGTCGTCG
ATGGCCTGTCGCGGCTTGGCGACAGGCGTTAG

Upstream 100 bases:

>100_bases
CGCGACATCCTGCTTCTCCGCGTCACAACCGCGGCCTGAGTGGACGACTTGAACTTCGCCAGTCAGGATGGCTCTCACGA
CGGCAAGGAGACACTTTCGG

Downstream 100 bases:

>100_bases
ACCACAGTAGTCTACAGTCTTCAGCGGGCTACCCGCGTCTCCAGCCCGGCGTGAGGCGCATAGGCGCTCGGCTGGAAAGC
ATCATGTTCGCGGCGCGCCA

Product: hypothetical protein

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MIKATPFDFPYDGRLVAENTALIVIDLQQDFLSTTGYFARKGYDPSPLRAILPTVNWLIAASRKAGVRVIHTRQGYRADM
ADMTPYEKWRRKRSGLDGTDVLLRSGAGFQIVPEIDVAPQDIIVDKTCNSAFTYTDFELVLRAQGITHLMFSGCTTDVCV
HTTLREACDRNFQCLTISDACASGDQKAHEAALHMVTVEDGVFGVLADSAAVVDGLSRLGDRR

Sequences:

>Translated_223_residues
MIKATPFDFPYDGRLVAENTALIVIDLQQDFLSTTGYFARKGYDPSPLRAILPTVNWLIAASRKAGVRVIHTRQGYRADM
ADMTPYEKWRRKRSGLDGTDVLLRSGAGFQIVPEIDVAPQDIIVDKTCNSAFTYTDFELVLRAQGITHLMFSGCTTDVCV
HTTLREACDRNFQCLTISDACASGDQKAHEAALHMVTVEDGVFGVLADSAAVVDGLSRLGDRR
>Mature_223_residues
MIKATPFDFPYDGRLVAENTALIVIDLQQDFLSTTGYFARKGYDPSPLRAILPTVNWLIAASRKAGVRVIHTRQGYRADM
ADMTPYEKWRRKRSGLDGTDVLLRSGAGFQIVPEIDVAPQDIIVDKTCNSAFTYTDFELVLRAQGITHLMFSGCTTDVCV
HTTLREACDRNFQCLTISDACASGDQKAHEAALHMVTVEDGVFGVLADSAAVVDGLSRLGDRR

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=211, Percent_Identity=29.3838862559242, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI87081992, Length=195, Percent_Identity=27.1794871794872, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 24551; Mature: 24551

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKATPFDFPYDGRLVAENTALIVIDLQQDFLSTTGYFARKGYDPSPLRAILPTVNWLIA
CCCCCCCCCCCCCEEEECCCEEEEEECCHHHHHHCCHHHHCCCCCCHHHHHHHHHHHEEE
ASRKAGVRVIHTRQGYRADMADMTPYEKWRRKRSGLDGTDVLLRSGAGFQIVPEIDVAPQ
ECCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHEECCCCEEEEECCCCCCC
DIIVDKTCNSAFTYTDFELVLRAQGITHLMFSGCTTDVCVHTTLREACDRNFQCLTISDA
CEEEECCCCCCEEECHHHHEEECCCHHEEHHCCCCHHHHHHHHHHHHHCCCCEEEEECHH
CASGDQKAHEAALHMVTVEDGVFGVLADSAAVVDGLSRLGDRR
HCCCCHHHHHHEEEEEEECCCEEEEHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIKATPFDFPYDGRLVAENTALIVIDLQQDFLSTTGYFARKGYDPSPLRAILPTVNWLIA
CCCCCCCCCCCCCEEEECCCEEEEEECCHHHHHHCCHHHHCCCCCCHHHHHHHHHHHEEE
ASRKAGVRVIHTRQGYRADMADMTPYEKWRRKRSGLDGTDVLLRSGAGFQIVPEIDVAPQ
ECCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHHHHEECCCCEEEEECCCCCCC
DIIVDKTCNSAFTYTDFELVLRAQGITHLMFSGCTTDVCVHTTLREACDRNFQCLTISDA
CEEEECCCCCCEEECHHHHEEECCCHHEEHHCCCCHHHHHHHHHHHHHCCCCEEEEECHH
CASGDQKAHEAALHMVTVEDGVFGVLADSAAVVDGLSRLGDRR
HCCCCHHHHHHEEEEEEECCCEEEEHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA