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The map label for this gene is ogt [H]

Identifier: 13472284

GI number: 13472284

Start: 2051211

End: 2051780

Strand: Direct

Name: ogt [H]

Synonym: mlr2526

Alternate gene names: 13472284

Gene position: 2051211-2051780 (Clockwise)

Preceding gene: 13472276

Following gene: 13472286

Centisome position: 29.15

GC content: 67.02

Gene sequence:

>570_bases
ATGGAAACGACACCGTCGATCACAGCCGGCCACGCAGTGTTAGAAACAGTGATCGGTTTCATGGGCATCGCCTGGAGCGA
AAAGGGCCTGATCCGGCTCTGCCTGCCCGAACGCAGCCGTGAATCAGTGGAGCGGCGGCTGATGCGCCATGCCGGCGTTT
CCGCCTCCACCGCGCAGCCGCCATGGGTGGTCGAGCTGATTGCCTCGATCAAGGCTTACGCCGCGGGCGAGGACGTCGAT
TTCTCCAATGTGCCGGTCGATCTCGACGGCGTCGACGATTTCCGCCTGGCCATCTATGACGCGGCACGCAAACTCAGCTA
TGGCGAGACAACCACCTATGGCGAACTCGCCAAGCGCGCCGGCCATGCCGGCCTGCCGCGCGAGACGGGAGCGGCCCTTG
GCGCCAACCCCGTGCCGCTGGTCATCCCCTGCCACCGCATCCTGGCGGCCGGCGGCAAGATCGGCGGCTTCTCGGCGCCC
GGCGGCTCCGTCACCAAGGAAAAGATGCTGGCCATGGAAGGCGTGCGCGTCGGCCCGCCGCCGCCGGCGCAGGTGTCCTT
CGGCTTCTGA

Upstream 100 bases:

>100_bases
TGGTTTTCGGGTGGCGAGACGCGGGCGGTGATGCCATATTGTTCCTGATATGTACTGGCAGGAATCCCGGATTTCGCCGC
TTTGCGGAAAGGAGCGCATC

Downstream 100 bases:

>100_bases
ACCGTGGTGCGGACGCGCTGCCCCTAGTTGTTGTGGAAGAACCGCCGGCGGAAATGCCGGTCATTGTCCGGCCGCTTGCA
GTTGTAGACCGGACAGGACT

Product: methylated-DNA-protein-cystein methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 189; Mature: 189

Protein sequence:

>189_residues
METTPSITAGHAVLETVIGFMGIAWSEKGLIRLCLPERSRESVERRLMRHAGVSASTAQPPWVVELIASIKAYAAGEDVD
FSNVPVDLDGVDDFRLAIYDAARKLSYGETTTYGELAKRAGHAGLPRETGAALGANPVPLVIPCHRILAAGGKIGGFSAP
GGSVTKEKMLAMEGVRVGPPPPAQVSFGF

Sequences:

>Translated_189_residues
METTPSITAGHAVLETVIGFMGIAWSEKGLIRLCLPERSRESVERRLMRHAGVSASTAQPPWVVELIASIKAYAAGEDVD
FSNVPVDLDGVDDFRLAIYDAARKLSYGETTTYGELAKRAGHAGLPRETGAALGANPVPLVIPCHRILAAGGKIGGFSAP
GGSVTKEKMLAMEGVRVGPPPPAQVSFGF
>Mature_189_residues
METTPSITAGHAVLETVIGFMGIAWSEKGLIRLCLPERSRESVERRLMRHAGVSASTAQPPWVVELIASIKAYAAGEDVD
FSNVPVDLDGVDDFRLAIYDAARKLSYGETTTYGELAKRAGHAGLPRETGAALGANPVPLVIPCHRILAAGGKIGGFSAP
GGSVTKEKMLAMEGVRVGPPPPAQVSFGF

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=86, Percent_Identity=39.5348837209302, Blast_Score=70, Evalue=9e-13,
Organism=Escherichia coli, GI1787596, Length=109, Percent_Identity=33.0275229357798, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1 [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19869; Mature: 19869

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00435 PEROXIDASE_1 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METTPSITAGHAVLETVIGFMGIAWSEKGLIRLCLPERSRESVERRLMRHAGVSASTAQP
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCC
PWVVELIASIKAYAAGEDVDFSNVPVDLDGVDDFRLAIYDAARKLSYGETTTYGELAKRA
CHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHC
GHAGLPRETGAALGANPVPLVIPCHRILAAGGKIGGFSAPGGSVTKEKMLAMEGVRVGPP
CCCCCCCCCCCCCCCCCCEEEEHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCC
PPAQVSFGF
CCCCCCCCC
>Mature Secondary Structure
METTPSITAGHAVLETVIGFMGIAWSEKGLIRLCLPERSRESVERRLMRHAGVSASTAQP
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCC
PWVVELIASIKAYAAGEDVDFSNVPVDLDGVDDFRLAIYDAARKLSYGETTTYGELAKRA
CHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHC
GHAGLPRETGAALGANPVPLVIPCHRILAAGGKIGGFSAPGGSVTKEKMLAMEGVRVGPP
CCCCCCCCCCCCCCCCCCEEEEHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCC
PPAQVSFGF
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]