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The map label for this gene is 13472228

Identifier: 13472228

GI number: 13472228

Start: 1993490

End: 1994143

Strand: Direct

Name: 13472228

Synonym: mlr2452

Alternate gene names: NA

Gene position: 1993490-1994143 (Clockwise)

Preceding gene: 13472225

Following gene: 13472231

Centisome position: 28.33

GC content: 58.56

Gene sequence:

>654_bases
ATGATTGAACGTCTTGCCACCGGAAGTGCATTGCTTGGCGCAGCGCTTGCGAGGATTCAAGACGGCACGATGCGCTACAC
GTACAAGGGCGTGCCCACCTACAAAAATCCATTCGATCTGGCGCTGTACCAAATGCTGCTTTGGCAGCAAAAGCCCCGGA
CATTGATCGAGATCGGCTCCAAATGGGGCGGCAGCGCGCTCTGGTTTGCCGATATGATGTGCAGCTTCGGCGTCGACTGC
GTCATTCACTCGATCGACATCACGCCGCCCTCGATCAGCGTACCGGGCGTGACGTTTCATCGTGGCGACGGTCGCGACCT
GGCGGCAACCCTACCGGCGGATCTGATGGAGAGCCTCCCCAGGCCGATTTTCGTCATCGAGGACGCGGACCATCATTGCG
AGACGACGCTCGCCGTGCTGCGCTTCTTCGATCGTTGGCTGGTTGCCGGAGAGTATATTGCCGTCGAGGACGGTATTGTA
GACGATCTTTATGGTCCAGAATTTGTCGCCAGGCTGATGGGCGGCCCGCGTCGTGCGGTCGAGCTGTTCTTGCGCGATCG
TGGGCAGAACTATGAAATCGATACCGGCCTTTGCGACCACTTCGGCACCAATGTGACGTGGAACGTCAACGGCTATCTCC
GTCGCGTTCGCTAA

Upstream 100 bases:

>100_bases
TTCGGCAAGCATCTTCTGCACCGCCAACCGGCCCAATCACGCTTTGTCGCTGGCGGCATACCGGATAATTGGGTTTGACG
TCGGCTTGTAGGACAACCGT

Downstream 100 bases:

>100_bases
CAACGCTCATTCCTTGCGCCACCAGGATCCGGTCCAGTCGACCTCTTGCATCGGCGCCGTGATGCCGTGTTTATCGCGAA
AATCGATCACCGCGCGCCGG

Product: cephalosporin hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MIERLATGSALLGAALARIQDGTMRYTYKGVPTYKNPFDLALYQMLLWQQKPRTLIEIGSKWGGSALWFADMMCSFGVDC
VIHSIDITPPSISVPGVTFHRGDGRDLAATLPADLMESLPRPIFVIEDADHHCETTLAVLRFFDRWLVAGEYIAVEDGIV
DDLYGPEFVARLMGGPRRAVELFLRDRGQNYEIDTGLCDHFGTNVTWNVNGYLRRVR

Sequences:

>Translated_217_residues
MIERLATGSALLGAALARIQDGTMRYTYKGVPTYKNPFDLALYQMLLWQQKPRTLIEIGSKWGGSALWFADMMCSFGVDC
VIHSIDITPPSISVPGVTFHRGDGRDLAATLPADLMESLPRPIFVIEDADHHCETTLAVLRFFDRWLVAGEYIAVEDGIV
DDLYGPEFVARLMGGPRRAVELFLRDRGQNYEIDTGLCDHFGTNVTWNVNGYLRRVR
>Mature_217_residues
MIERLATGSALLGAALARIQDGTMRYTYKGVPTYKNPFDLALYQMLLWQQKPRTLIEIGSKWGGSALWFADMMCSFGVDC
VIHSIDITPPSISVPGVTFHRGDGRDLAATLPADLMESLPRPIFVIEDADHHCETTLAVLRFFDRWLVAGEYIAVEDGIV
DDLYGPEFVARLMGGPRRAVELFLRDRGQNYEIDTGLCDHFGTNVTWNVNGYLRRVR

Specific function: Catalyzes the O-methylation of the hydroxyl group located on C-2 of the first rhamnosyl residue linked to the phenolic group of glycosylated phenolphthiocerol dimycocerosates (PGL) and p-hydroxybenzoic acid derivatives (p-HBAD) [H]

COG id: COG3510

COG function: function code V; Cephalosporin hydroxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rhamnosyl O-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007072 [H]

Pfam domain/function: PF04989 CmcI [H]

EC number: NA

Molecular weight: Translated: 24359; Mature: 24359

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERLATGSALLGAALARIQDGTMRYTYKGVPTYKNPFDLALYQMLLWQQKPRTLIEIGS
CCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHCC
KWGGSALWFADMMCSFGVDCVIHSIDITPPSISVPGVTFHRGDGRDLAATLPADLMESLP
CCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCEEEECCCCCCCHHHCCHHHHHHCC
RPIFVIEDADHHCETTLAVLRFFDRWLVAGEYIAVEDGIVDDLYGPEFVARLMGGPRRAV
CCEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHCCCHHHHHHHHCCHHHHH
ELFLRDRGQNYEIDTGLCDHFGTNVTWNVNGYLRRVR
HHHHHHCCCCEEECCCCHHHCCCCEEEECCCEEEECC
>Mature Secondary Structure
MIERLATGSALLGAALARIQDGTMRYTYKGVPTYKNPFDLALYQMLLWQQKPRTLIEIGS
CCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHCC
KWGGSALWFADMMCSFGVDCVIHSIDITPPSISVPGVTFHRGDGRDLAATLPADLMESLP
CCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCEEEECCCCCCCHHHCCHHHHHHCC
RPIFVIEDADHHCETTLAVLRFFDRWLVAGEYIAVEDGIVDDLYGPEFVARLMGGPRRAV
CCEEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHCCCHHHHHHHHCCHHHHH
ELFLRDRGQNYEIDTGLCDHFGTNVTWNVNGYLRRVR
HHHHHHCCCCEEECCCCHHHCCCCEEEECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]