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The map label for this gene is phaZ1 [H]

Identifier: 13472223

GI number: 13472223

Start: 1987205

End: 1988320

Strand: Direct

Name: phaZ1 [H]

Synonym: mlr2444

Alternate gene names: 13472223

Gene position: 1987205-1988320 (Clockwise)

Preceding gene: 13472222

Following gene: 13472225

Centisome position: 28.24

GC content: 63.8

Gene sequence:

>1116_bases
ATGCGAGGCCGGACGGCATTTTCCCAACAAGGCCATGCCGACAGTCTGTCCGACCTCGCCGAGTTCGGATCCAATCCGGG
CGCCTTGCGGGCGCGTTTCTACGCACCCGAGGACTTGCCGAAGGGAGCAGCCTTGGTTGTCGTCCTGCACGGCTGCACGC
AGACCGCGGCGGGCTACGATCACGGCGCTGGCTGGTCGCGCCTGGCGGCGGAGGAAGGGTTTGCTGTATTGTTCCCGGAA
CAGCAGCGAGCCAACAATGCCAATCTCTGCTTCAACTGGTTCGTGCCCGAAGATGTAACCCGCGATCGCGGTGAAGCGCT
CTCCATCCGCCGGATGATCGAAGCCATGGTGACGAAGCATGGCCTGGACCGAGAGCGCATTTTCATCACCGGTCTTTCCG
CAGGCGGCGCCATGGCAGCGGCAATGCTGGCAACTTATCCGGACGTGTTTGCGGGTGGCGCGATCATTGCGGGCCTTGCC
TATGGCAGCGCATCGACGATCCCCGAAGCGTTCGATCGCATGCGTGGGCATGGTGGCCCAACCCCGGCGGAGCTCCACCG
ACGCCTGGCGCAGGCTTCGCCCCATCAGGGAAGCTGGCCGAGGATTTCGATCTGGCAGGGCACGGCCGATCACACGGTGG
CGCCGTCCAACGCCGACTCACTTGTCGCCCAATGGCAAAGCGTTCACCGCGTCGGGACAGGCCCTGATCGAGCAGAGACG
GTCGATCGACAAACGCGGCAGGTCTGGTGCGACGCCGATGGCAATGAAGTGATCGAAAAATACACCATTGCCGGCATGGG
CCATGGCACTCCCTTGAAGACGGGGGGCGAGGACGGCCTTGGCAATGCCGCGCCGTTCATGCTGGATGTCGGGATATCGT
CGACGCGCCGGATGGTGGCCTTCTGGAAGCTCGCACGCCGGGATGCTCGAGGGGAGGCCCAGCCTGCTACCGGCTCGGCC
CATCCTGCCGAGGGCACAGCTTTGCGCACATACATTGCGCCCGAAAAATCGAGGCCAGCTGCCGAACAACCGCGTCCAGA
TGGCTCACGTGCTTCTCCTACAGCCATTGGCAAGGTGATCGAGGATGCGCTTCGCGCAGCCGGCTTGATGCGTTAG

Upstream 100 bases:

>100_bases
CGATGTTGCTGGAAGTGCATTGTGCTCGGGCTTCCGCCACTCCGGCGGCTAGGATTTCGATTTGCGGAATATCTCAGATA
CGATCGCTCGCCTTTCGGCC

Downstream 100 bases:

>100_bases
AGCGTTTCACCGTTTCATGGAAACGGCGAATCCTCTAACTCTTTGTTTAGACGCAATTCCCAAGGGAAGCGCTACGCGCT
TTTCCCGGGAAAACCGCTTA

Product: polyhydroxybutyrate depolymerase

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MRGRTAFSQQGHADSLSDLAEFGSNPGALRARFYAPEDLPKGAALVVVLHGCTQTAAGYDHGAGWSRLAAEEGFAVLFPE
QQRANNANLCFNWFVPEDVTRDRGEALSIRRMIEAMVTKHGLDRERIFITGLSAGGAMAAAMLATYPDVFAGGAIIAGLA
YGSASTIPEAFDRMRGHGGPTPAELHRRLAQASPHQGSWPRISIWQGTADHTVAPSNADSLVAQWQSVHRVGTGPDRAET
VDRQTRQVWCDADGNEVIEKYTIAGMGHGTPLKTGGEDGLGNAAPFMLDVGISSTRRMVAFWKLARRDARGEAQPATGSA
HPAEGTALRTYIAPEKSRPAAEQPRPDGSRASPTAIGKVIEDALRAAGLMR

Sequences:

>Translated_371_residues
MRGRTAFSQQGHADSLSDLAEFGSNPGALRARFYAPEDLPKGAALVVVLHGCTQTAAGYDHGAGWSRLAAEEGFAVLFPE
QQRANNANLCFNWFVPEDVTRDRGEALSIRRMIEAMVTKHGLDRERIFITGLSAGGAMAAAMLATYPDVFAGGAIIAGLA
YGSASTIPEAFDRMRGHGGPTPAELHRRLAQASPHQGSWPRISIWQGTADHTVAPSNADSLVAQWQSVHRVGTGPDRAET
VDRQTRQVWCDADGNEVIEKYTIAGMGHGTPLKTGGEDGLGNAAPFMLDVGISSTRRMVAFWKLARRDARGEAQPATGSA
HPAEGTALRTYIAPEKSRPAAEQPRPDGSRASPTAIGKVIEDALRAAGLMR
>Mature_371_residues
MRGRTAFSQQGHADSLSDLAEFGSNPGALRARFYAPEDLPKGAALVVVLHGCTQTAAGYDHGAGWSRLAAEEGFAVLFPE
QQRANNANLCFNWFVPEDVTRDRGEALSIRRMIEAMVTKHGLDRERIFITGLSAGGAMAAAMLATYPDVFAGGAIIAGLA
YGSASTIPEAFDRMRGHGGPTPAELHRRLAQASPHQGSWPRISIWQGTADHTVAPSNADSLVAQWQSVHRVGTGPDRAET
VDRQTRQVWCDADGNEVIEKYTIAGMGHGTPLKTGGEDGLGNAAPFMLDVGISSTRRMVAFWKLARRDARGEAQPATGSA
HPAEGTALRTYIAPEKSRPAAEQPRPDGSRASPTAIGKVIEDALRAAGLMR

Specific function: Specific for poly(hydroxyalkanoic acid) consisting of monomers of four or five carbon atoms and for P- nitrophenylbutyrate as substrates [H]

COG id: COG3509

COG function: function code Q; Poly(3-hydroxybutyrate) depolymerase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010126 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 39532; Mature: 39532

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGRTAFSQQGHADSLSDLAEFGSNPGALRARFYAPEDLPKGAALVVVLHGCTQTAAGYD
CCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEEEECCCHHCCCCC
HGAGWSRLAAEEGFAVLFPEQQRANNANLCFNWFVPEDVTRDRGEALSIRRMIEAMVTKH
CCCCHHHHHHHCCCEEECCCHHCCCCCCEEEEECCCCHHHHCCCHHHHHHHHHHHHHHHC
GLDRERIFITGLSAGGAMAAAMLATYPDVFAGGAIIAGLAYGSASTIPEAFDRMRGHGGP
CCCCCEEEEEECCCCHHHHHHHHHHCHHHHHCCHHHHHHHCCCCCCHHHHHHHHHCCCCC
TPAELHRRLAQASPHQGSWPRISIWQGTADHTVAPSNADSLVAQWQSVHRVGTGPDRAET
CHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHH
VDRQTRQVWCDADGNEVIEKYTIAGMGHGTPLKTGGEDGLGNAAPFMLDVGISSTRRMVA
HHHHHHEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHH
FWKLARRDARGEAQPATGSAHPAEGTALRTYIAPEKSRPAAEQPRPDGSRASPTAIGKVI
HHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHH
EDALRAAGLMR
HHHHHHHCCCC
>Mature Secondary Structure
MRGRTAFSQQGHADSLSDLAEFGSNPGALRARFYAPEDLPKGAALVVVLHGCTQTAAGYD
CCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEEEECCCHHCCCCC
HGAGWSRLAAEEGFAVLFPEQQRANNANLCFNWFVPEDVTRDRGEALSIRRMIEAMVTKH
CCCCHHHHHHHCCCEEECCCHHCCCCCCEEEEECCCCHHHHCCCHHHHHHHHHHHHHHHC
GLDRERIFITGLSAGGAMAAAMLATYPDVFAGGAIIAGLAYGSASTIPEAFDRMRGHGGP
CCCCCEEEEEECCCCHHHHHHHHHHCHHHHHCCHHHHHHHCCCCCCHHHHHHHHHCCCCC
TPAELHRRLAQASPHQGSWPRISIWQGTADHTVAPSNADSLVAQWQSVHRVGTGPDRAET
CHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHH
VDRQTRQVWCDADGNEVIEKYTIAGMGHGTPLKTGGEDGLGNAAPFMLDVGISSTRRMVA
HHHHHHEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHH
FWKLARRDARGEAQPATGSAHPAEGTALRTYIAPEKSRPAAEQPRPDGSRASPTAIGKVI
HHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHH
EDALRAAGLMR
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8269961 [H]