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The map label for this gene is rbsB [H]

Identifier: 13471994

GI number: 13471994

Start: 1747126

End: 1748133

Strand: Reverse

Name: rbsB [H]

Synonym: mll2148

Alternate gene names: 13471994

Gene position: 1748133-1747126 (Counterclockwise)

Preceding gene: 13471998

Following gene: 13471993

Centisome position: 24.85

GC content: 64.48

Gene sequence:

>1008_bases
ATGATCCTTCCTGAAACCGTCCCGGTTTCCGGGGCGTGCGTTCTGTGGAGGAGATCAACCATGAACAGACGTGAATTCCT
GCTGACGTCCGTGGCCGCCGGCGCCATGGTGCTTGCCGCACCTTCGGTGTTCGCCGAGGACAAGCTGACCATACTGGGCT
CGGTGCCCAATCTCGGCTTTCCGTTCTTCGTGCACATGCTGAACGAGATCAAGGCCGAGGCGCAGGCGCAAGGCGTCAAC
CTGACCGAAAGCGACGGCCAGAATTCGGCCACCAAGCAGACCGCAGACATCGAGGCGGCACTGGTGCAGAAGGTCAACGC
CATCGTGATTTCGCCGCTCGACGTCAACGCGCTGGCGCCGGCGATCGAGGAGGCGGTCAAGGCCGGTGTTCCCGTGGTGA
CGATCGACCGCCGCGTCGACGGTGTCCAGGGCATTCTCGCCCATGTCGGCGCCGACAATGTCAAGGGCGGCGAGGCCGAG
GCCAGCGCCATGGTGGCGGCGTTCCCCAACGGCGCCAAGCTGTTCCATCTGCAGGGCCAGCCGGGCGCCGGTCCGGCGAT
CGACCGCAACAAGGGCGTGCACAATGTGCTCGATCCCCTGAAGGACAAGTACCAGATCATCTTCGAGCAGACCGCAAACT
TTGCCCGCGCCGAAGCGCTGTCGGTGACGGAAGCCGGCCTCGCCGCCAATGGCAAGCCGGACGCCATCATCTGCGCCAAT
GACGACATGGCGCTCGGCGCGCTCGAGGCCTGTGCGGCGCGCAACTTCACCGACGTCAAGATCTACGGCTTCGACGCGCT
GCCGGAAGCGCTGGTTGCCGTGCGCGACGGCAAGCTCGCCGGCACGGTCGAACAGTTCCCCGGCCAGCAGTCGCGCACCG
CGGTGCAGATCGCGGTCGCCTACGCCAAGAACAAGACCGAGCCGAAGGAAAAGCTGGTGCTGTTGACGCCCATCGTCATC
GGCAAGGACAATCTTGATAAGGCGGAGAGGTTGAGCGAGACGAAGTAG

Upstream 100 bases:

>100_bases
TGCTGACGCCGATGGGAGGCAGTTTTTTGCAACGGCGATCAGCTGGAGCGGCATGCGCTGTCCCGCGCAAGGAGGATGCT
CCAACACTTTGAACCGACGC

Downstream 100 bases:

>100_bases
GGGGCTTGTGAAAGCTGAGCGCATGAAGGGGGGACGTCGCGTTCTGTCGCGCCCCCCTCTGTCCTGCCGGACATCTCCCC
CACGAGGGGGGAGATCGACC

Product: ribose-binding protein of ribose ABC transporter

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MILPETVPVSGACVLWRRSTMNRREFLLTSVAAGAMVLAAPSVFAEDKLTILGSVPNLGFPFFVHMLNEIKAEAQAQGVN
LTESDGQNSATKQTADIEAALVQKVNAIVISPLDVNALAPAIEEAVKAGVPVVTIDRRVDGVQGILAHVGADNVKGGEAE
ASAMVAAFPNGAKLFHLQGQPGAGPAIDRNKGVHNVLDPLKDKYQIIFEQTANFARAEALSVTEAGLAANGKPDAIICAN
DDMALGALEACAARNFTDVKIYGFDALPEALVAVRDGKLAGTVEQFPGQQSRTAVQIAVAYAKNKTEPKEKLVLLTPIVI
GKDNLDKAERLSETK

Sequences:

>Translated_335_residues
MILPETVPVSGACVLWRRSTMNRREFLLTSVAAGAMVLAAPSVFAEDKLTILGSVPNLGFPFFVHMLNEIKAEAQAQGVN
LTESDGQNSATKQTADIEAALVQKVNAIVISPLDVNALAPAIEEAVKAGVPVVTIDRRVDGVQGILAHVGADNVKGGEAE
ASAMVAAFPNGAKLFHLQGQPGAGPAIDRNKGVHNVLDPLKDKYQIIFEQTANFARAEALSVTEAGLAANGKPDAIICAN
DDMALGALEACAARNFTDVKIYGFDALPEALVAVRDGKLAGTVEQFPGQQSRTAVQIAVAYAKNKTEPKEKLVLLTPIVI
GKDNLDKAERLSETK
>Mature_335_residues
MILPETVPVSGACVLWRRSTMNRREFLLTSVAAGAMVLAAPSVFAEDKLTILGSVPNLGFPFFVHMLNEIKAEAQAQGVN
LTESDGQNSATKQTADIEAALVQKVNAIVISPLDVNALAPAIEEAVKAGVPVVTIDRRVDGVQGILAHVGADNVKGGEAE
ASAMVAAFPNGAKLFHLQGQPGAGPAIDRNKGVHNVLDPLKDKYQIIFEQTANFARAEALSVTEAGLAANGKPDAIICAN
DDMALGALEACAARNFTDVKIYGFDALPEALVAVRDGKLAGTVEQFPGQQSRTAVQIAVAYAKNKTEPKEKLVLLTPIVI
GKDNLDKAERLSETK

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=278, Percent_Identity=33.0935251798561, Blast_Score=140, Evalue=8e-35,
Organism=Escherichia coli, GI1790674, Length=257, Percent_Identity=31.5175097276265, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1788473, Length=328, Percent_Identity=29.8780487804878, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1789990, Length=271, Percent_Identity=30.6273062730627, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1790526, Length=293, Percent_Identity=26.962457337884, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35270; Mature: 35270

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILPETVPVSGACVLWRRSTMNRREFLLTSVAAGAMVLAAPSVFAEDKLTILGSVPNLGF
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCEEEEECCCHHCCCCEEEEECCCCCCH
PFFVHMLNEIKAEAQAQGVNLTESDGQNSATKQTADIEAALVQKVNAIVISPLDVNALAP
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCHHHHH
AIEEAVKAGVPVVTIDRRVDGVQGILAHVGADNVKGGEAEASAMVAAFPNGAKLFHLQGQ
HHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCHHCEEEEECCCCCEEEEEECC
PGAGPAIDRNKGVHNVLDPLKDKYQIIFEQTANFARAEALSVTEAGLAANGKPDAIICAN
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEECC
DDMALGALEACAARNFTDVKIYGFDALPEALVAVRDGKLAGTVEQFPGQQSRTAVQIAVA
CCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCCCCCCHHHCCCCCCCCEEEEEEE
YAKNKTEPKEKLVLLTPIVIGKDNLDKAERLSETK
ECCCCCCHHHCEEEEEEEEECCCCCCHHHHHHCCC
>Mature Secondary Structure
MILPETVPVSGACVLWRRSTMNRREFLLTSVAAGAMVLAAPSVFAEDKLTILGSVPNLGF
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCEEEEECCCHHCCCCEEEEECCCCCCH
PFFVHMLNEIKAEAQAQGVNLTESDGQNSATKQTADIEAALVQKVNAIVISPLDVNALAP
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCHHHHH
AIEEAVKAGVPVVTIDRRVDGVQGILAHVGADNVKGGEAEASAMVAAFPNGAKLFHLQGQ
HHHHHHHCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCHHCEEEEECCCCCEEEEEECC
PGAGPAIDRNKGVHNVLDPLKDKYQIIFEQTANFARAEALSVTEAGLAANGKPDAIICAN
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEECC
DDMALGALEACAARNFTDVKIYGFDALPEALVAVRDGKLAGTVEQFPGQQSRTAVQIAVA
CCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCCCCCCCHHHCCCCCCCCEEEEEEE
YAKNKTEPKEKLVLLTPIVIGKDNLDKAERLSETK
ECCCCCCHHHCEEEEEEEEECCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]