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The map label for this gene is mtkB [H]

Identifier: 13471374

GI number: 13471374

Start: 1111919

End: 1112818

Strand: Direct

Name: mtkB [H]

Synonym: mlr1326

Alternate gene names: 13471374

Gene position: 1111919-1112818 (Clockwise)

Preceding gene: 13471373

Following gene: 13471378

Centisome position: 15.8

GC content: 66.33

Gene sequence:

>900_bases
ATGGCAATCCTGCTCAACCGCAGCACCCGCGTCATCGTCCAGGGTTTCACCGGCAAGATCGGCAGCTTCCACGCCGAGGA
CATGAAACGCTATGGCACCAAGCTGGTCGGCGGCGTCACCCCCGGCAAGGGCGGCCAGACGCATCTCGGCCTGCCCGTCT
TCAACACGGTCAAGGGCGCCGTGCGCGAGACCCGCGCCGAGGCGAGCATCGTCTTCGTGCCGCCACCCTTCGCCGCCGAT
TCGATCATGGAAGCCGCGGACGCCGGCATCAAACTCTGCGTCTGCATCACCGACGGCATCCCCTCGCAGGACATGATGCA
GGTCAAGCGCTACATGCGCCGCTATCGTTTCGAGGACCGCATGAGGCTGATCGGCCCGAACTGCGCCGGCATGATCACGC
CGGGACAGGCACTGATGGGCATCATGCCGGGCAGCATCTATCTGCCCGGCCGCGTCGGCATTGTCGGCCGCTCCGGCACA
CTCGGCTACGAGGCCGCCTCGCAGATGAAGGCGCTTGGCATCGGCGTGTCGACCAGCGTCGGCATCGGCGGCGACCCGAT
CAACGGCTCCTCCTTCAAGGACATCCTGAAGCTGTTCGAGCAGGACGACGAGACCGACGCCGTGGTGATGATCGGCGAGA
TCGGCGGCCCGCAAGAGGCGGAAGCCGCGATCTGGGCGCGCGACAACATGCGCAAGCCGCTTATCGCCTACATTGCCGGC
CTCTCCGCCCCGAAAGGCCGCCGCATGGGCCACGCCGGCGCCATCATCTCCGCCTTCGGCGAGTCGGCGCAGGAGAAGGT
CGAGATCCTGAAGGAGGCCGGCGTGATCATCGTGCCGACACCGTCATCGTTTGGCGAGGTGGTGGCGGATACGCTGGCAC
GGATGAAGAAGGCGGCGTGA

Upstream 100 bases:

>100_bases
CGGTGATCGTCGCCGACACGCTGGCCGAAGCCGCCGAAAAAGCCGTCGCCGCCTGGCGCGCGGCCGCCAAGAAAAAAGCA
GCCTGAGGGAGGTCGAGAAA

Downstream 100 bases:

>100_bases
TTTCCGCGCCTCTGCACGCTGGGGGTCCCTAACATACGCCCGCGCGAGCCTCCCCGGTCATAGGAGAGTCGGCGATCACC
CTTGCCGGGCGCCGCTCGCC

Product: succinyl-CoA synthetase subunit alpha

Products: NA

Alternate protein names: MTK-alpha; Malate thiokinase; Malyl-CoA synthetase [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MAILLNRSTRVIVQGFTGKIGSFHAEDMKRYGTKLVGGVTPGKGGQTHLGLPVFNTVKGAVRETRAEASIVFVPPPFAAD
SIMEAADAGIKLCVCITDGIPSQDMMQVKRYMRRYRFEDRMRLIGPNCAGMITPGQALMGIMPGSIYLPGRVGIVGRSGT
LGYEAASQMKALGIGVSTSVGIGGDPINGSSFKDILKLFEQDDETDAVVMIGEIGGPQEAEAAIWARDNMRKPLIAYIAG
LSAPKGRRMGHAGAIISAFGESAQEKVEILKEAGVIIVPTPSSFGEVVADTLARMKKAA

Sequences:

>Translated_299_residues
MAILLNRSTRVIVQGFTGKIGSFHAEDMKRYGTKLVGGVTPGKGGQTHLGLPVFNTVKGAVRETRAEASIVFVPPPFAAD
SIMEAADAGIKLCVCITDGIPSQDMMQVKRYMRRYRFEDRMRLIGPNCAGMITPGQALMGIMPGSIYLPGRVGIVGRSGT
LGYEAASQMKALGIGVSTSVGIGGDPINGSSFKDILKLFEQDDETDAVVMIGEIGGPQEAEAAIWARDNMRKPLIAYIAG
LSAPKGRRMGHAGAIISAFGESAQEKVEILKEAGVIIVPTPSSFGEVVADTLARMKKAA
>Mature_298_residues
AILLNRSTRVIVQGFTGKIGSFHAEDMKRYGTKLVGGVTPGKGGQTHLGLPVFNTVKGAVRETRAEASIVFVPPPFAADS
IMEAADAGIKLCVCITDGIPSQDMMQVKRYMRRYRFEDRMRLIGPNCAGMITPGQALMGIMPGSIYLPGRVGIVGRSGTL
GYEAASQMKALGIGVSTSVGIGGDPINGSSFKDILKLFEQDDETDAVVMIGEIGGPQEAEAAIWARDNMRKPLIAYIAGL
SAPKGRRMGHAGAIISAFGESAQEKVEILKEAGVIIVPTPSSFGEVVADTLARMKKAA

Specific function: Unknown

COG id: COG0074

COG function: function code C; Succinyl-CoA synthetase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the succinate/malate CoA ligase alpha subunit family [H]

Homologues:

Organism=Homo sapiens, GI109452591, Length=298, Percent_Identity=52.3489932885906, Blast_Score=275, Evalue=4e-74,
Organism=Homo sapiens, GI38569423, Length=257, Percent_Identity=30.3501945525292, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI38569421, Length=257, Percent_Identity=30.3501945525292, Blast_Score=92, Evalue=7e-19,
Organism=Escherichia coli, GI1786949, Length=292, Percent_Identity=57.1917808219178, Blast_Score=331, Evalue=4e-92,
Organism=Caenorhabditis elegans, GI17550194, Length=289, Percent_Identity=49.8269896193772, Blast_Score=272, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI17553126, Length=287, Percent_Identity=48.780487804878, Blast_Score=267, Evalue=4e-72,
Organism=Caenorhabditis elegans, GI17551266, Length=258, Percent_Identity=28.2945736434109, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17557344, Length=282, Percent_Identity=27.6595744680851, Blast_Score=91, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6324716, Length=302, Percent_Identity=46.0264900662252, Blast_Score=268, Evalue=8e-73,
Organism=Drosophila melanogaster, GI21356559, Length=296, Percent_Identity=47.6351351351351, Blast_Score=283, Evalue=1e-76,
Organism=Drosophila melanogaster, GI28574296, Length=296, Percent_Identity=48.6486486486487, Blast_Score=254, Evalue=5e-68,
Organism=Drosophila melanogaster, GI281363475, Length=270, Percent_Identity=26.2962962962963, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI281363473, Length=270, Percent_Identity=26.2962962962963, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI281363477, Length=270, Percent_Identity=26.2962962962963, Blast_Score=88, Evalue=6e-18,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017440
- InterPro:   IPR003781
- InterPro:   IPR005810
- InterPro:   IPR005811
- InterPro:   IPR016040
- InterPro:   IPR016102 [H]

Pfam domain/function: PF02629 CoA_binding; PF00549 Ligase_CoA [H]

EC number: =6.2.1.9 [H]

Molecular weight: Translated: 31601; Mature: 31470

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: PS00399 SUCCINYL_COA_LIG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAILLNRSTRVIVQGFTGKIGSFHAEDMKRYGTKLVGGVTPGKGGQTHLGLPVFNTVKGA
CEEEEECCCEEEEEECCCCCCCCCHHHHHHHCHHHCCCCCCCCCCCEEECCCHHHHHHHH
VRETRAEASIVFVPPPFAADSIMEAADAGIKLCVCITDGIPSQDMMQVKRYMRRYRFEDR
HHHHHCCCEEEEECCCCCHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHCHHHH
MRLIGPNCAGMITPGQALMGIMPGSIYLPGRVGIVGRSGTLGYEAASQMKALGIGVSTSV
HHEECCCCCCEECCHHHHHHCCCCCEECCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCC
GIGGDPINGSSFKDILKLFEQDDETDAVVMIGEIGGPQEAEAAIWARDNMRKPLIAYIAG
CCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHC
LSAPKGRRMGHAGAIISAFGESAQEKVEILKEAGVIIVPTPSSFGEVVADTLARMKKAA
CCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AILLNRSTRVIVQGFTGKIGSFHAEDMKRYGTKLVGGVTPGKGGQTHLGLPVFNTVKGA
EEEEECCCEEEEEECCCCCCCCCHHHHHHHCHHHCCCCCCCCCCCEEECCCHHHHHHHH
VRETRAEASIVFVPPPFAADSIMEAADAGIKLCVCITDGIPSQDMMQVKRYMRRYRFEDR
HHHHHCCCEEEEECCCCCHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHCHHHH
MRLIGPNCAGMITPGQALMGIMPGSIYLPGRVGIVGRSGTLGYEAASQMKALGIGVSTSV
HHEECCCCCCEECCHHHHHHCCCCCEECCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCC
GIGGDPINGSSFKDILKLFEQDDETDAVVMIGEIGGPQEAEAAIWARDNMRKPLIAYIAG
CCCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHC
LSAPKGRRMGHAGAIISAFGESAQEKVEILKEAGVIIVPTPSSFGEVVADTLARMKKAA
CCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7961516 [H]