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The map label for this gene is mcbR [H]

Identifier: 13471149

GI number: 13471149

Start: 873598

End: 874278

Strand: Direct

Name: mcbR [H]

Synonym: mlr1038

Alternate gene names: 13471149

Gene position: 873598-874278 (Clockwise)

Preceding gene: 13471143

Following gene: 13471150

Centisome position: 12.42

GC content: 63.14

Gene sequence:

>681_bases
ATGGCCGGAGCGGCGTTCAGGAAGCCTGGTGTCGAGATCGTGCCCCTGTCCAGGGAGACCTTGCAGGACAGGGTCTATCG
CCATGTCACCGAATTGATCCTCGACGGCAGCATCGTGCCCGGCGAGATGGTGACGGTGCAAAGCCTCGCCGACGCCTTTG
GGGTCAGTCCGATGCCGGTCAGGGAGGCGTTGCGGCGGCTGACTGCGGCCAATGCCTTGATGGTCGTTTCAGGGCGCTCG
ATCGGCATTCCCGCACTCAGCCGCGCGCGCCTCATCGATCTCAGGAATGTCCGCTTCGAGATCGAGGCGATCGCCGCCGC
CTGGGCGGCCGAGCGCATCGACGATCAGGGCATCGCGCAGCTCGGCCAGCATCTGGCAGCGCTGGAACAGGCCAATGCGG
CGGGAGACGTCAAATCCTATCTGCGCGCCAACTATGCCTTCCATTTCTCCATCTACCGGGCGGCCGGCTCGGAAAACATC
CTCAACATCATCGAGAATCTGTGGCTGCAGATCAGCCCCTATTTCAACATGCTGCATGATTCCGGAAACTATTCGACCGC
CAACGAGCACCACCAGGCGATGTTCGCGGCGCTGCGCAACCGCGACGCCGAGGCCGTCCGCCAGGCGATCCGCGCCGATA
TCGACGCCGCTTTCGACGTTCTGGTCGACCTGCTCAAATAG

Upstream 100 bases:

>100_bases
CATTCGAGCCGCGATCGCCAACGTTTGACGCGGCAGCCGGAACCCTGCATTCCGGGAACGGGTTGTGGCGCGGCCTTTGC
GCCGAAAGGTTTGATGGGAA

Downstream 100 bases:

>100_bases
GTCGCGGCGGCAAAAGACGCGATTTTTTCCGTCAGGATAGTCGCCTCTGGAATTGACCCCAAATTAAGCTATGATGCGCT
TGGTATTTCTTGGCGGCGGC

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MAGAAFRKPGVEIVPLSRETLQDRVYRHVTELILDGSIVPGEMVTVQSLADAFGVSPMPVREALRRLTAANALMVVSGRS
IGIPALSRARLIDLRNVRFEIEAIAAAWAAERIDDQGIAQLGQHLAALEQANAAGDVKSYLRANYAFHFSIYRAAGSENI
LNIIENLWLQISPYFNMLHDSGNYSTANEHHQAMFAALRNRDAEAVRQAIRADIDAAFDVLVDLLK

Sequences:

>Translated_226_residues
MAGAAFRKPGVEIVPLSRETLQDRVYRHVTELILDGSIVPGEMVTVQSLADAFGVSPMPVREALRRLTAANALMVVSGRS
IGIPALSRARLIDLRNVRFEIEAIAAAWAAERIDDQGIAQLGQHLAALEQANAAGDVKSYLRANYAFHFSIYRAAGSENI
LNIIENLWLQISPYFNMLHDSGNYSTANEHHQAMFAALRNRDAEAVRQAIRADIDAAFDVLVDLLK
>Mature_225_residues
AGAAFRKPGVEIVPLSRETLQDRVYRHVTELILDGSIVPGEMVTVQSLADAFGVSPMPVREALRRLTAANALMVVSGRSI
GIPALSRARLIDLRNVRFEIEAIAAAWAAERIDDQGIAQLGQHLAALEQANAAGDVKSYLRANYAFHFSIYRAAGSENIL
NIIENLWLQISPYFNMLHDSGNYSTANEHHQAMFAALRNRDAEAVRQAIRADIDAAFDVLVDLLK

Specific function: Important for biofilm formation. Represses expression of mcbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081914, Length=178, Percent_Identity=31.4606741573034, Blast_Score=112, Evalue=2e-26,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 24837; Mature: 24705

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGAAFRKPGVEIVPLSRETLQDRVYRHVTELILDGSIVPGEMVTVQSLADAFGVSPMPV
CCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCH
REALRRLTAANALMVVSGRSIGIPALSRARLIDLRNVRFEIEAIAAAWAAERIDDQGIAQ
HHHHHHHHHHCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LGQHLAALEQANAAGDVKSYLRANYAFHFSIYRAAGSENILNIIENLWLQISPYFNMLHD
HHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHEEHHHHHHHHHC
SGNYSTANEHHQAMFAALRNRDAEAVRQAIRADIDAAFDVLVDLLK
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
AGAAFRKPGVEIVPLSRETLQDRVYRHVTELILDGSIVPGEMVTVQSLADAFGVSPMPV
CCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCH
REALRRLTAANALMVVSGRSIGIPALSRARLIDLRNVRFEIEAIAAAWAAERIDDQGIAQ
HHHHHHHHHHCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LGQHLAALEQANAAGDVKSYLRANYAFHFSIYRAAGSENILNIIENLWLQISPYFNMLHD
HHHHHHHHHHCCCHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHEEHHHHHHHHHC
SGNYSTANEHHQAMFAALRNRDAEAVRQAIRADIDAAFDVLVDLLK
CCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]