Click here to switch to the map view.

The map label for this gene is emrB [H]

Identifier: 13471110

GI number: 13471110

Start: 820237

End: 821850

Strand: Reverse

Name: emrB [H]

Synonym: mll0994

Alternate gene names: 13471110

Gene position: 821850-820237 (Counterclockwise)

Preceding gene: 13471111

Following gene: 13471107

Centisome position: 11.68

GC content: 62.08

Gene sequence:

>1614_bases
ATGACAAGCATCGCGTCATCCATGCCGGCTCCGGCTCGACCTGCCCGGAACGAGACCGGAAGTGCTGGCACTGCCGCTTC
ACCGCGCGTCTGGGTGGCGGTGGTCGGCTCCTCCCTCGGCGCTTTCATGGCTGTGCTGAACATTCAGATCGTCAACGCCT
CGCTGGCCGACATCCAGGGCGCCATCGGAGCCGGCATCGACGATGGCGGCTGGATCTCGACCGCCTATCTCATCGCGGAG
ATCGTGGTAATCCCGCTGACGGGATGGCTGTCCCAGGTCTTCTCGCTGCGCAACTATCTCATCGCCAACGCAGTACTATT
CCTCGCCTTTTCAGTGGCTTGTGCCTTCGCCGCCAACCTCGGGCAGATGATCGTCTTGAGGGCCATCCAGGGTTTCACTG
GCGGCGTGCTCATCCCGATGGCCTTCACCATCGTTATCACCCTGCTACCCAAGGCGAGACAGCCGATCGGACTGGCGCTC
TTCGCAATGTCAGCAACCTTCGCGCCAGCGATAGGCCCGACGATCGGCGGCTACCTCACCGAGAACTGGGGCTGGCAGTA
TATCTTCTACGTCAACATCGTGCCCGGCGCGCTGATGGTCGGCATGCTGTGGTTCTCGCTCGACCGCCAGCCGATGCGGC
TCGCCTTGCTCGGCGAGGGCGACTGGCCCGGAATCGCGACCATGGCCATCGGCCTCGCCGCGCTGCAGACGGTGCTCGAG
GAAGGCAACAAGAACGACTGGTTCGGCTCGCTTTTCGTCCTGCGGCTGGCAATCGTCGCGGCCGTGTCGCTCTCGCTGTT
CGTCTGGATCGAACTCACATCGAGCCATCCGCTGCTCAACCTGAGGCTGCTCCTGCGTCGCAATTTCGGCTTCGGCATCC
TGGCCAATTTCATGCTGGGGACGGCGCTCTACGGCTCGGTCTTCATCCTCCCGGTGTATCTCGCGCGGATCCAAGGTTAC
AATGCCGAGCAGATCGGCTTGGTGCTCGCATGGACCGGCCTGCCGCAGCTGTTGCTGATCCCGCTGGTTCCCTACCTGAT
GCAGCGCTTCGACGTGCGCCTTGTGATTGTGACCGGCTTCGCACTCTTCGCCGCCTCGAATTTCATGAACGTGCACATGA
CCGCCGGCTACGCCAGTGACCAATTGTTCTGGCCGAACATTGTGCGGGCGATCGGCCAGGCCCTGGTTTTCGCACCGCTC
TCGGCGATCGCAGTATCGGGCATCGAGCAGGAGAACGCCGGCTCGGCATCGTCTTTGTTCAACATGATCCGCAACCTCGG
CGGCGCCGTCGGCATCGCGCTGCTGCAGACCTTCCTGACCAAGCGCCAGCAGTATCATTCGAACGTGCTGGTGCAGTCGA
TCTCATTGTTCGAAGAGGCAACGCGTTCCCGCATCGCGAAGCTCACCGCCTATTTCCTGTCGCATGGAGTCAGTGATCAC
GTGCTCGCCGCACAGAAAGGGGTGATCGCGATTGCGCTGAGAGTGAGGCAGCAGGCCTTCATTATGGCTTTCAGCGACAC
TTTCTTCCTGCTCGGGGTTGCCCTGGTGATCGCGCTCGCGGCCACTCTGCTTCTGAAGAAACCCGGCCATATTGAAGCCG
GCGGCGCGCACTGA

Upstream 100 bases:

>100_bases
GGTGCTTGCCGCGGATGGCCTGTCCGCACGGCCCGCCACGCTATCCATCAATTGGAACACCGAGGCCGCGACGAAGCGCG
CCGAGTAGGCATTAGCAGCC

Downstream 100 bases:

>100_bases
GCGGCTTGGCCGACGCGATCCGGTATGCGCACGAAGCGGCGTGGCGAAAGCGACCGCAAGCGTCACCAGAAGGCGATTGA
CGGTCGGAAGGAATAGCCGA

Product: transport protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MTSIASSMPAPARPARNETGSAGTAASPRVWVAVVGSSLGAFMAVLNIQIVNASLADIQGAIGAGIDDGGWISTAYLIAE
IVVIPLTGWLSQVFSLRNYLIANAVLFLAFSVACAFAANLGQMIVLRAIQGFTGGVLIPMAFTIVITLLPKARQPIGLAL
FAMSATFAPAIGPTIGGYLTENWGWQYIFYVNIVPGALMVGMLWFSLDRQPMRLALLGEGDWPGIATMAIGLAALQTVLE
EGNKNDWFGSLFVLRLAIVAAVSLSLFVWIELTSSHPLLNLRLLLRRNFGFGILANFMLGTALYGSVFILPVYLARIQGY
NAEQIGLVLAWTGLPQLLLIPLVPYLMQRFDVRLVIVTGFALFAASNFMNVHMTAGYASDQLFWPNIVRAIGQALVFAPL
SAIAVSGIEQENAGSASSLFNMIRNLGGAVGIALLQTFLTKRQQYHSNVLVQSISLFEEATRSRIAKLTAYFLSHGVSDH
VLAAQKGVIAIALRVRQQAFIMAFSDTFFLLGVALVIALAATLLLKKPGHIEAGGAH

Sequences:

>Translated_537_residues
MTSIASSMPAPARPARNETGSAGTAASPRVWVAVVGSSLGAFMAVLNIQIVNASLADIQGAIGAGIDDGGWISTAYLIAE
IVVIPLTGWLSQVFSLRNYLIANAVLFLAFSVACAFAANLGQMIVLRAIQGFTGGVLIPMAFTIVITLLPKARQPIGLAL
FAMSATFAPAIGPTIGGYLTENWGWQYIFYVNIVPGALMVGMLWFSLDRQPMRLALLGEGDWPGIATMAIGLAALQTVLE
EGNKNDWFGSLFVLRLAIVAAVSLSLFVWIELTSSHPLLNLRLLLRRNFGFGILANFMLGTALYGSVFILPVYLARIQGY
NAEQIGLVLAWTGLPQLLLIPLVPYLMQRFDVRLVIVTGFALFAASNFMNVHMTAGYASDQLFWPNIVRAIGQALVFAPL
SAIAVSGIEQENAGSASSLFNMIRNLGGAVGIALLQTFLTKRQQYHSNVLVQSISLFEEATRSRIAKLTAYFLSHGVSDH
VLAAQKGVIAIALRVRQQAFIMAFSDTFFLLGVALVIALAATLLLKKPGHIEAGGAH
>Mature_536_residues
TSIASSMPAPARPARNETGSAGTAASPRVWVAVVGSSLGAFMAVLNIQIVNASLADIQGAIGAGIDDGGWISTAYLIAEI
VVIPLTGWLSQVFSLRNYLIANAVLFLAFSVACAFAANLGQMIVLRAIQGFTGGVLIPMAFTIVITLLPKARQPIGLALF
AMSATFAPAIGPTIGGYLTENWGWQYIFYVNIVPGALMVGMLWFSLDRQPMRLALLGEGDWPGIATMAIGLAALQTVLEE
GNKNDWFGSLFVLRLAIVAAVSLSLFVWIELTSSHPLLNLRLLLRRNFGFGILANFMLGTALYGSVFILPVYLARIQGYN
AEQIGLVLAWTGLPQLLLIPLVPYLMQRFDVRLVIVTGFALFAASNFMNVHMTAGYASDQLFWPNIVRAIGQALVFAPLS
AIAVSGIEQENAGSASSLFNMIRNLGGAVGIALLQTFLTKRQQYHSNVLVQSISLFEEATRSRIAKLTAYFLSHGVSDHV
LAAQKGVIAIALRVRQQAFIMAFSDTFFLLGVALVIALAATLLLKKPGHIEAGGAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=498, Percent_Identity=28.3132530120482, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1788710, Length=536, Percent_Identity=29.2910447761194, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI87081983, Length=420, Percent_Identity=25.952380952381, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1790195, Length=438, Percent_Identity=24.8858447488584, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788392, Length=454, Percent_Identity=23.568281938326, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6322958, Length=187, Percent_Identity=24.0641711229947, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6325455, Length=149, Percent_Identity=25.503355704698, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 57458; Mature: 57326

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSIASSMPAPARPARNETGSAGTAASPRVWVAVVGSSLGAFMAVLNIQIVNASLADIQG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHEECCHHHHHHH
AIGAGIDDGGWISTAYLIAEIVVIPLTGWLSQVFSLRNYLIANAVLFLAFSVACAFAANL
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GQMIVLRAIQGFTGGVLIPMAFTIVITLLPKARQPIGLALFAMSATFAPAIGPTIGGYLT
HHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEE
ENWGWQYIFYVNIVPGALMVGMLWFSLDRQPMRLALLGEGDWPGIATMAIGLAALQTVLE
CCCCEEEEEEEEEHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHH
EGNKNDWFGSLFVLRLAIVAAVSLSLFVWIELTSSHPLLNLRLLLRRNFGFGILANFMLG
CCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHH
TALYGSVFILPVYLARIQGYNAEQIGLVLAWTGLPQLLLIPLVPYLMQRFDVRLVIVTGF
HHHHHHHHHHHHHHHHHCCCCCHHEEEEEEECCCHHHHHHHHHHHHHHHHCEEEEEEHHH
ALFAASNFMNVHMTAGYASDQLFWPNIVRAIGQALVFAPLSAIAVSGIEQENAGSASSLF
HHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
NMIRNLGGAVGIALLQTFLTKRQQYHSNVLVQSISLFEEATRSRIAKLTAYFLSHGVSDH
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
VLAAQKGVIAIALRVRQQAFIMAFSDTFFLLGVALVIALAATLLLKKPGHIEAGGAH
HHHHHCCHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TSIASSMPAPARPARNETGSAGTAASPRVWVAVVGSSLGAFMAVLNIQIVNASLADIQG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHEECCHHHHHHH
AIGAGIDDGGWISTAYLIAEIVVIPLTGWLSQVFSLRNYLIANAVLFLAFSVACAFAANL
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GQMIVLRAIQGFTGGVLIPMAFTIVITLLPKARQPIGLALFAMSATFAPAIGPTIGGYLT
HHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEE
ENWGWQYIFYVNIVPGALMVGMLWFSLDRQPMRLALLGEGDWPGIATMAIGLAALQTVLE
CCCCEEEEEEEEEHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHH
EGNKNDWFGSLFVLRLAIVAAVSLSLFVWIELTSSHPLLNLRLLLRRNFGFGILANFMLG
CCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHH
TALYGSVFILPVYLARIQGYNAEQIGLVLAWTGLPQLLLIPLVPYLMQRFDVRLVIVTGF
HHHHHHHHHHHHHHHHHCCCCCHHEEEEEEECCCHHHHHHHHHHHHHHHHCEEEEEEHHH
ALFAASNFMNVHMTAGYASDQLFWPNIVRAIGQALVFAPLSAIAVSGIEQENAGSASSLF
HHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
NMIRNLGGAVGIALLQTFLTKRQQYHSNVLVQSISLFEEATRSRIAKLTAYFLSHGVSDH
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
VLAAQKGVIAIALRVRQQAFIMAFSDTFFLLGVALVIALAATLLLKKPGHIEAGGAH
HHHHHCCHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]