| Definition | Mesorhizobium loti MAFF303099 chromosome, complete genome. |
|---|---|
| Accession | NC_002678 |
| Length | 7,036,071 |
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The map label for this gene is topA [H]
Identifier: 13470988
GI number: 13470988
Start: 668879
End: 671500
Strand: Direct
Name: topA [H]
Synonym: mlr0839
Alternate gene names: 13470988
Gene position: 668879-671500 (Clockwise)
Preceding gene: 13470985
Following gene: 13470989
Centisome position: 9.51
GC content: 64.04
Gene sequence:
>2622_bases ATGGACGTCGTCGTCGTCGAATCGCCGGCCAAGGCGAAGACAATCAACAAATACCTCGGCAAGAACTACAAGGTTCTGGC CTCGTTCGGCCATGTCCGCGATTTGCCGGCCAAGGATGGCTCGGTGCGCCCGGATGAAGATTTCGCCATGTCCTGGGCGG TCGACACCGCCTCGGGCAAGCGGCTCGCCGACATCGCCAAGGCGGTCAAGGATGCCGACGGCCTGATCCTCGCCACCGAC CCGGATCGCGAAGGCGAGGCCATTTCCTGGCATGTTCTGGAAGTGCTGAAGCAGAAGCGCGCGCTGAAGGACAAGCCGGT CAGCCGGGTCGTCTTCAACGCCATCACCAAATCGTCGGTGCTGGAAGCCATGGCCAATCCGCGCCAGATCGACGCGCCGC TGGTCGATGCCTATCTGGCCCGCCGGGCGCTGGACTATCTCGTCGGCTTCACGCTGTCGCCGGTGCTGTGGCGCAAATTG CCCGGCGCCCGCTCGGCCGGCCGCGTCCAGTCCGTGGCCTTGCGTCTGGTCTGCGACCGTGAATCCGAAATCGAACGCTT CATCCGCGAGGAATATTGGCAGATCGCCGCCATCCTCGGCACGCCGCGCAACGAGACTTTCGAGGCGCGGCTGACCGCCT TCGAGCGCAAGAAGCTGCAAAAGCTCGACATTGCCAACAAGGCGCAGGCCGACGACATCAAGGCGATGCTCGAGGGCGCG ACCTTCAAGGCGTTGTCGGTGGAAGCCAAGCCGACCAAGCGCAACCCAGGCCCGCCCTTCACCACCTCGACCTTGCAGCA GGCCGCCTCCTCGGGCCTCGGTTTTTCGGCCACCCGCACCATGCAGGTGGCGCAGCGGCTCTATGAGGGTATGGAGATCG GCGGCGAAACGACCGGCCTGATCACCTATATGCGAACAGACGGCGTGCAGATGGCGCCCGAGGCTATCGACGCTGCCCGC GATGCCATCGCCAAGGAATTCGGCCCGAAATATCTGCCGGAAAAGCCGCGTTTCTACACGACCAAGGCCAAGAATGCCCA GGAAGCGCACGAGGCGATCCGTCCGACGGATTTCATGCGCACTCCGGCATCGGTTCGGCAGTATCTCGATTCCGACCAGA TGCGGCTTTATGAGCTGATCTGGAAGCGCGCCATCGCCAGCCAGATGCAGCCGGCCGAAATCGAGCGCACCACAGTCGAG ATCGAGGCCGTCAATGGCGCCCGCACAGCCGAACTTCGCGCTGTCGGTTCGGTCGTTCGCTTCGACGGCTTCATCGCCGC CTACACCGATCAGAAGGACGACGACGCGGAGGACGAGGAAAACCGCCGTCTGCCGGAAATCCGCGCCGGCGAGCAGCTTG CCCGCCAGGCAATCAACGCCACCCAGCACACGACCGAGCCGCCACCGCGCTATTCCGAGGCCACGCTGATCAAGAAGCTG GAAGAACTCGGCATCGGCCGGCCGTCGACCTATACGGCAATCCTGAAGACGCTCGAGGACCGCGACTATGTCACGATCGA CAAGCGCCGGCTGGTGCCGCAGGCCAAGGGCCGCCTGCTGTCCGCTTTCCTCGAAAGCTTCTTCGAGCGCTATGTCGAAT ATGACTTCACGGCATCGCTGGAGGAAAAGCTCGACGAGATTTCCGACGGCAAGCTCGCCTGGAAGGACGTGCTGCGCGAT TTCTGGAAGGATTTTTCGGGCGCCGTCGCCGACATCAAGGAGCTGCGCGTCACCGATGTGCTCGATGCGCTGAACGAGGA ACTGGCGCCCCTGGTCTTCCCCGCGCGCGAAGACGGCTCCAACCCGCGGATCTGCCCGAAATGCGGCACCGGCAACCTCT CGCTGAAGCTCGGCAAGTTCGGCGCCTTCGTCGGCTGCTCGAACTATCCGGAATGCTCCTTCACCCGCCAGCTCGGCGAC GCCGCCAATCCTAATGGCGAGAATGGCGGCGGCGATGACGGCACCAAGGTGCTCGGCAAGGATCCCTACACGGCCGAGGA AATCACCCTGCGATCGGGTCGCTTCGGGCCCTACCTCCAGCGCGGCGACGGTAAGGACGCCAAGCGCTCGAGCCTGCCCA AGGGCTGGACGCCCGAAACCATCGACCATGAGAAAGCATTGGCGTTGCTGGCGCTGCCGCGCGATATCGGCAAGCATCCT GAAACGTCCAAGATGATCTCGGCCGGGCTCGGCCGCTACGGCCCGTTCGTGCTGCATGATGGCACCTATGCCAATCTCGA CAGCATCGAGGATGTGTTCTCGATCGGCCTCAACCGCGCCGTCACGGTGATCGCCGAGAAGCAGTCGAAGGGCAAGGGCG GCCGCAATGGCGGCACGCCGGCGGCGCTGAAGGAGCTTGGCGACCATCCGGACGGTGGCGGCAAGATCGTCGTGCGCGAC GGCAAATACGGGCCTTACGTCAATTTCGGCAAGGTCAATGCCACGCTGCCCAAGGGCAAGGATCCGCAATCGGTGACCAT CGAGGACGCGCTGGCGCTGATTGCCGAGAAGGAAGCCAAGGGCGGCGGCGGCAAGAAGCCGTTCCGCAAGGCAGCCGCGG CCAAGGCGCCCGCGGCCAAGAAAACAGCCGCCAGGAAGCCGGCGGCGAAGAAAAAAGGGTAG
Upstream 100 bases:
>100_bases GACCGGATGCAAAAGCGCGGCCATGTGAGCGGCAAATAGAACGAGATTTCGTCGCGCGCAGCGGAATTCTTCTGCCGCAT CACAGCTGATATGAGACCTC
Downstream 100 bases:
>100_bases GACGGCGTGGCGCGCAGGATCACCGGACGGAGCCACGGCGATCCACGCACCGCCGACACGAGGGCCAAGGTCAAGGACGA CTATCGGCCTTCGCGCGACG
Product: DNA topoisomerase I
Products: NA
Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]
Number of amino acids: Translated: 873; Mature: 873
Protein sequence:
>873_residues MDVVVVESPAKAKTINKYLGKNYKVLASFGHVRDLPAKDGSVRPDEDFAMSWAVDTASGKRLADIAKAVKDADGLILATD PDREGEAISWHVLEVLKQKRALKDKPVSRVVFNAITKSSVLEAMANPRQIDAPLVDAYLARRALDYLVGFTLSPVLWRKL PGARSAGRVQSVALRLVCDRESEIERFIREEYWQIAAILGTPRNETFEARLTAFERKKLQKLDIANKAQADDIKAMLEGA TFKALSVEAKPTKRNPGPPFTTSTLQQAASSGLGFSATRTMQVAQRLYEGMEIGGETTGLITYMRTDGVQMAPEAIDAAR DAIAKEFGPKYLPEKPRFYTTKAKNAQEAHEAIRPTDFMRTPASVRQYLDSDQMRLYELIWKRAIASQMQPAEIERTTVE IEAVNGARTAELRAVGSVVRFDGFIAAYTDQKDDDAEDEENRRLPEIRAGEQLARQAINATQHTTEPPPRYSEATLIKKL EELGIGRPSTYTAILKTLEDRDYVTIDKRRLVPQAKGRLLSAFLESFFERYVEYDFTASLEEKLDEISDGKLAWKDVLRD FWKDFSGAVADIKELRVTDVLDALNEELAPLVFPAREDGSNPRICPKCGTGNLSLKLGKFGAFVGCSNYPECSFTRQLGD AANPNGENGGGDDGTKVLGKDPYTAEEITLRSGRFGPYLQRGDGKDAKRSSLPKGWTPETIDHEKALALLALPRDIGKHP ETSKMISAGLGRYGPFVLHDGTYANLDSIEDVFSIGLNRAVTVIAEKQSKGKGGRNGGTPAALKELGDHPDGGGKIVVRD GKYGPYVNFGKVNATLPKGKDPQSVTIEDALALIAEKEAKGGGGKKPFRKAAAAKAPAAKKTAARKPAAKKKG
Sequences:
>Translated_873_residues MDVVVVESPAKAKTINKYLGKNYKVLASFGHVRDLPAKDGSVRPDEDFAMSWAVDTASGKRLADIAKAVKDADGLILATD PDREGEAISWHVLEVLKQKRALKDKPVSRVVFNAITKSSVLEAMANPRQIDAPLVDAYLARRALDYLVGFTLSPVLWRKL PGARSAGRVQSVALRLVCDRESEIERFIREEYWQIAAILGTPRNETFEARLTAFERKKLQKLDIANKAQADDIKAMLEGA TFKALSVEAKPTKRNPGPPFTTSTLQQAASSGLGFSATRTMQVAQRLYEGMEIGGETTGLITYMRTDGVQMAPEAIDAAR DAIAKEFGPKYLPEKPRFYTTKAKNAQEAHEAIRPTDFMRTPASVRQYLDSDQMRLYELIWKRAIASQMQPAEIERTTVE IEAVNGARTAELRAVGSVVRFDGFIAAYTDQKDDDAEDEENRRLPEIRAGEQLARQAINATQHTTEPPPRYSEATLIKKL EELGIGRPSTYTAILKTLEDRDYVTIDKRRLVPQAKGRLLSAFLESFFERYVEYDFTASLEEKLDEISDGKLAWKDVLRD FWKDFSGAVADIKELRVTDVLDALNEELAPLVFPAREDGSNPRICPKCGTGNLSLKLGKFGAFVGCSNYPECSFTRQLGD AANPNGENGGGDDGTKVLGKDPYTAEEITLRSGRFGPYLQRGDGKDAKRSSLPKGWTPETIDHEKALALLALPRDIGKHP ETSKMISAGLGRYGPFVLHDGTYANLDSIEDVFSIGLNRAVTVIAEKQSKGKGGRNGGTPAALKELGDHPDGGGKIVVRD GKYGPYVNFGKVNATLPKGKDPQSVTIEDALALIAEKEAKGGGGKKPFRKAAAAKAPAAKKTAARKPAAKKKG >Mature_873_residues MDVVVVESPAKAKTINKYLGKNYKVLASFGHVRDLPAKDGSVRPDEDFAMSWAVDTASGKRLADIAKAVKDADGLILATD PDREGEAISWHVLEVLKQKRALKDKPVSRVVFNAITKSSVLEAMANPRQIDAPLVDAYLARRALDYLVGFTLSPVLWRKL PGARSAGRVQSVALRLVCDRESEIERFIREEYWQIAAILGTPRNETFEARLTAFERKKLQKLDIANKAQADDIKAMLEGA TFKALSVEAKPTKRNPGPPFTTSTLQQAASSGLGFSATRTMQVAQRLYEGMEIGGETTGLITYMRTDGVQMAPEAIDAAR DAIAKEFGPKYLPEKPRFYTTKAKNAQEAHEAIRPTDFMRTPASVRQYLDSDQMRLYELIWKRAIASQMQPAEIERTTVE IEAVNGARTAELRAVGSVVRFDGFIAAYTDQKDDDAEDEENRRLPEIRAGEQLARQAINATQHTTEPPPRYSEATLIKKL EELGIGRPSTYTAILKTLEDRDYVTIDKRRLVPQAKGRLLSAFLESFFERYVEYDFTASLEEKLDEISDGKLAWKDVLRD FWKDFSGAVADIKELRVTDVLDALNEELAPLVFPAREDGSNPRICPKCGTGNLSLKLGKFGAFVGCSNYPECSFTRQLGD AANPNGENGGGDDGTKVLGKDPYTAEEITLRSGRFGPYLQRGDGKDAKRSSLPKGWTPETIDHEKALALLALPRDIGKHP ETSKMISAGLGRYGPFVLHDGTYANLDSIEDVFSIGLNRAVTVIAEKQSKGKGGRNGGTPAALKELGDHPDGGGKIVVRD GKYGPYVNFGKVNATLPKGKDPQSVTIEDALALIAEKEAKGGGGKKPFRKAAAAKAPAAKKTAARKPAAKKKG
Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]
COG id: COG0550
COG function: function code L; Topoisomerase IA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 Toprim domain [H]
Homologues:
Organism=Homo sapiens, GI10835218, Length=626, Percent_Identity=25.2396166134185, Blast_Score=123, Evalue=7e-28, Organism=Homo sapiens, GI4507635, Length=633, Percent_Identity=24.1706161137441, Blast_Score=111, Evalue=3e-24, Organism=Escherichia coli, GI1787529, Length=663, Percent_Identity=38.9140271493213, Blast_Score=447, Evalue=1e-126, Organism=Escherichia coli, GI1788061, Length=539, Percent_Identity=26.3450834879406, Blast_Score=130, Evalue=4e-31, Organism=Caenorhabditis elegans, GI17555378, Length=510, Percent_Identity=27.4509803921569, Blast_Score=129, Evalue=8e-30, Organism=Caenorhabditis elegans, GI32563869, Length=570, Percent_Identity=27.1929824561404, Blast_Score=121, Evalue=2e-27, Organism=Saccharomyces cerevisiae, GI6323263, Length=556, Percent_Identity=24.2805755395683, Blast_Score=106, Evalue=1e-23, Organism=Drosophila melanogaster, GI24585251, Length=614, Percent_Identity=27.5244299674267, Blast_Score=144, Evalue=2e-34, Organism=Drosophila melanogaster, GI24640096, Length=568, Percent_Identity=26.056338028169, Blast_Score=120, Evalue=4e-27,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003601 - InterPro: IPR013497 - InterPro: IPR013824 - InterPro: IPR013825 - InterPro: IPR000380 - InterPro: IPR003602 - InterPro: IPR013498 - InterPro: IPR005733 - InterPro: IPR006171 [H]
Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]
EC number: =5.99.1.2 [H]
Molecular weight: Translated: 95624; Mature: 95624
Theoretical pI: Translated: 9.25; Mature: 9.25
Prosite motif: PS00396 TOPOISOMERASE_I_PROK
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.6 %Cys (Translated Protein) 1.4 %Met (Translated Protein) 1.9 %Cys+Met (Translated Protein) 0.6 %Cys (Mature Protein) 1.4 %Met (Mature Protein) 1.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MDVVVVESPAKAKTINKYLGKNYKVLASFGHVRDLPAKDGSVRPDEDFAMSWAVDTASGK CCEEEECCCHHHHHHHHHHCCCEEEEECCCCHHCCCCCCCCCCCCCCCEEEEEEECCCCC RLADIAKAVKDADGLILATDPDREGEAISWHVLEVLKQKRALKDKPVSRVVFNAITKSSV HHHHHHHHHHCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH LEAMANPRQIDAPLVDAYLARRALDYLVGFTLSPVLWRKLPGARSAGRVQSVALRLVCDR HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC ESEIERFIREEYWQIAAILGTPRNETFEARLTAFERKKLQKLDIANKAQADDIKAMLEGA HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCC TFKALSVEAKPTKRNPGPPFTTSTLQQAASSGLGFSATRTMQVAQRLYEGMEIGGETTGL CEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEE ITYMRTDGVQMAPEAIDAARDAIAKEFGPKYLPEKPRFYTTKAKNAQEAHEAIRPTDFMR EEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHCCCHHHHC TPASVRQYLDSDQMRLYELIWKRAIASQMQPAEIERTTVEIEAVNGARTAELRAVGSVVR CHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHEEEEEEEEECCCCCHHHHHHHHHHHH FDGFIAAYTDQKDDDAEDEENRRLPEIRAGEQLARQAINATQHTTEPPPRYSEATLIKKL HCCEEEEECCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH EELGIGRPSTYTAILKTLEDRDYVTIDKRRLVPQAKGRLLSAFLESFFERYVEYDFTASL HHHCCCCCHHHHHHHHHHCCCCCEEEHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHH EEKLDEISDGKLAWKDVLRDFWKDFSGAVADIKELRVTDVLDALNEELAPLVFPAREDGS HHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCC NPRICPKCGTGNLSLKLGKFGAFVGCSNYPECSFTRQLGDAANPNGENGGGDDGTKVLGK CCCCCCCCCCCCEEEEECCCEEEECCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCEECCC DPYTAEEITLRSGRFGPYLQRGDGKDAKRSSLPKGWTPETIDHEKALALLALPRDIGKHP CCCCHHHEEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHCCCC ETSKMISAGLGRYGPFVLHDGTYANLDSIEDVFSIGLNRAVTVIAEKQSKGKGGRNGGTP CHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCH AALKELGDHPDGGGKIVVRDGKYGPYVNFGKVNATLPKGKDPQSVTIEDALALIAEKEAK HHHHHCCCCCCCCCEEEEECCCCCCCEECCCEEEECCCCCCCCCEEHHHHHHHHHHHHCC GGGGKKPFRKAAAAKAPAAKKTAARKPAAKKKG CCCCCCHHHHHHHHCCCCHHHHHHCCCCCCCCC >Mature Secondary Structure MDVVVVESPAKAKTINKYLGKNYKVLASFGHVRDLPAKDGSVRPDEDFAMSWAVDTASGK CCEEEECCCHHHHHHHHHHCCCEEEEECCCCHHCCCCCCCCCCCCCCCEEEEEEECCCCC RLADIAKAVKDADGLILATDPDREGEAISWHVLEVLKQKRALKDKPVSRVVFNAITKSSV HHHHHHHHHHCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH LEAMANPRQIDAPLVDAYLARRALDYLVGFTLSPVLWRKLPGARSAGRVQSVALRLVCDR HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC ESEIERFIREEYWQIAAILGTPRNETFEARLTAFERKKLQKLDIANKAQADDIKAMLEGA HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCC TFKALSVEAKPTKRNPGPPFTTSTLQQAASSGLGFSATRTMQVAQRLYEGMEIGGETTGL CEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEE ITYMRTDGVQMAPEAIDAARDAIAKEFGPKYLPEKPRFYTTKAKNAQEAHEAIRPTDFMR EEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHCCCHHHHC TPASVRQYLDSDQMRLYELIWKRAIASQMQPAEIERTTVEIEAVNGARTAELRAVGSVVR CHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHEEEEEEEEECCCCCHHHHHHHHHHHH FDGFIAAYTDQKDDDAEDEENRRLPEIRAGEQLARQAINATQHTTEPPPRYSEATLIKKL HCCEEEEECCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH EELGIGRPSTYTAILKTLEDRDYVTIDKRRLVPQAKGRLLSAFLESFFERYVEYDFTASL HHHCCCCCHHHHHHHHHHCCCCCEEEHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHH EEKLDEISDGKLAWKDVLRDFWKDFSGAVADIKELRVTDVLDALNEELAPLVFPAREDGS HHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCC NPRICPKCGTGNLSLKLGKFGAFVGCSNYPECSFTRQLGDAANPNGENGGGDDGTKVLGK CCCCCCCCCCCCEEEEECCCEEEECCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCEECCC DPYTAEEITLRSGRFGPYLQRGDGKDAKRSSLPKGWTPETIDHEKALALLALPRDIGKHP CCCCHHHEEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHCCCC ETSKMISAGLGRYGPFVLHDGTYANLDSIEDVFSIGLNRAVTVIAEKQSKGKGGRNGGTP CHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCH AALKELGDHPDGGGKIVVRDGKYGPYVNFGKVNATLPKGKDPQSVTIEDALALIAEKEAK HHHHHCCCCCCCCCEEEEECCCCCCCEECCCEEEECCCCCCCCCEEHHHHHHHHHHHHCC GGGGKKPFRKAAAAKAPAAKKTAARKPAAKKKG CCCCCCHHHHHHHHCCCCHHHHHHCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA