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The map label for this gene is gltX

Identifier: 13470967

GI number: 13470967

Start: 643049

End: 644422

Strand: Reverse

Name: gltX

Synonym: mll0813

Alternate gene names: 13470967

Gene position: 644422-643049 (Counterclockwise)

Preceding gene: 13470968

Following gene: 13470962

Centisome position: 9.16

GC content: 64.19

Gene sequence:

>1374_bases
ATGACAGTTACCGTTCGTTTCGCCCCGTCGCCGACCGGCCGCATCCATATCGGCAATGCGCGCACCGCTCTGTTCAACTG
GCTGTTTGCGCTCAACAACAAGGGCCGCTTCATCCAGCGCTTCGACGACACCGACATTGGCCGCTCGAAGCAGGAATTCG
CCGACGCCATCCTCTACGACCTACATTGGCTGGGCATTTTCCCGGATGCCACCGAATATCAGTCGCGGCGCTTCGAGGTC
TATGACGCGGCGGTCGAGCGGCTGAAGGCGGCTGGCGTTCTCTACGCCTGCTACGAAACGCCGGAAGAACTCGATCTGAG
GCGCAAGGTGCGCCGTACGCGCGGCCTGCCGCCGGTCTATGGCCGCGAGGCCTTGGCGCTGACACATGAGCAGGTAGCCG
AATACCAGGCCGATGGCCGCCGGCCGCACTGGCGCTTCCTGCTTCCCAATTTCACCGGCGATCCGCTGCAGCCCGAACGC
ACCGAGGTTCATTGGAAAGACCTGGTGCGTGGCGAGGAAACGGTCGATCTCGCTTCGCTCTCCGACCCAGTGCTGGTGCG
CGAGGACGGCACCTATCTCTACACGCTGCCATCCGTCGTCGACGACATCGAGATGGGCGTCAGCCACGTCATCAGGGGTG
ACGACCATGTCACCAACACCGGCGTGCAGATCGCCTTGTTCAAGGCGCTGGGCACCGAGCCGCCTGTCTTCGGCCACCAC
AATCTCTTGACCACCGTGTCAGGCGAGGGGCTCTCGAAGCGCACCGGGGCACTCTCCATCGAAAGCCTGCGCGAGGATGG
CATCGAGCCGATGGCCGTCGCGTCGCTCGCCGTGCTGGTCGGCACTTCGGAGAATGTCGCGGCCGCGCATGATCTCACCG
AACTTGCCGGCCATTTCGACCCGGCCGCGACCTCGAAATCATCGGCCAAGTTCGATCCGGACGAACTGTTCGTGCTCAAC
CGCGTGCTGATGCACCACATGCCGTTCGACGAGGCGCGCGACAGGCTGATGGTGCTGGGCATCTCGGGCGAACAGGCCGA
GCCGTTCTGGCTGGCGGTGCGCGGCAATCTCGACCGGCTCGCCGATGCGGTGGGCTGGTGGCGCATCCTGCGCGAGGGGC
CGCAGGACAGACCGGAGTTCTCCGACGACGATCGTGACTTCCTCGGACAGGCTTTCGAAGTCTTGCCCGAAGAGCCCTGG
AACGGAACCGTCTGGAAGGACTGGACCGGCAAGATCAGGGAAGCGACCGGGCGCAAGGGCAAGGGGCTGTTCATGCCGCT
GAGGCTTGCCCTTACTGGACTGCCTTCGGGGCCGGAGCTTGCAGATTTATTGCCGCTGATGGGTCGGGAAGGAACGTTGG
CCCGACGACCCTGA

Upstream 100 bases:

>100_bases
AGGGCGCTAGTCTTTTCATCGCGCGCATCATGTTCTATGTGTGCCGACCAGTGCGGGGGCGCATCTTGTCAATCACAAAG
GCATCCGGTACGCCCCGCTC

Downstream 100 bases:

>100_bases
CCTTGCGCTGCTCCGGCGGTAGTGCGGATACGGGCGACGTCACCGGCTTGGCGGAAAGCCGTTTGACAGCCTCGCGGTCG
AGCCCGCCCTCCCTGTTGGC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 2; GluRS 2

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MTVTVRFAPSPTGRIHIGNARTALFNWLFALNNKGRFIQRFDDTDIGRSKQEFADAILYDLHWLGIFPDATEYQSRRFEV
YDAAVERLKAAGVLYACYETPEELDLRRKVRRTRGLPPVYGREALALTHEQVAEYQADGRRPHWRFLLPNFTGDPLQPER
TEVHWKDLVRGEETVDLASLSDPVLVREDGTYLYTLPSVVDDIEMGVSHVIRGDDHVTNTGVQIALFKALGTEPPVFGHH
NLLTTVSGEGLSKRTGALSIESLREDGIEPMAVASLAVLVGTSENVAAAHDLTELAGHFDPAATSKSSAKFDPDELFVLN
RVLMHHMPFDEARDRLMVLGISGEQAEPFWLAVRGNLDRLADAVGWWRILREGPQDRPEFSDDDRDFLGQAFEVLPEEPW
NGTVWKDWTGKIREATGRKGKGLFMPLRLALTGLPSGPELADLLPLMGREGTLARRP

Sequences:

>Translated_457_residues
MTVTVRFAPSPTGRIHIGNARTALFNWLFALNNKGRFIQRFDDTDIGRSKQEFADAILYDLHWLGIFPDATEYQSRRFEV
YDAAVERLKAAGVLYACYETPEELDLRRKVRRTRGLPPVYGREALALTHEQVAEYQADGRRPHWRFLLPNFTGDPLQPER
TEVHWKDLVRGEETVDLASLSDPVLVREDGTYLYTLPSVVDDIEMGVSHVIRGDDHVTNTGVQIALFKALGTEPPVFGHH
NLLTTVSGEGLSKRTGALSIESLREDGIEPMAVASLAVLVGTSENVAAAHDLTELAGHFDPAATSKSSAKFDPDELFVLN
RVLMHHMPFDEARDRLMVLGISGEQAEPFWLAVRGNLDRLADAVGWWRILREGPQDRPEFSDDDRDFLGQAFEVLPEEPW
NGTVWKDWTGKIREATGRKGKGLFMPLRLALTGLPSGPELADLLPLMGREGTLARRP
>Mature_456_residues
TVTVRFAPSPTGRIHIGNARTALFNWLFALNNKGRFIQRFDDTDIGRSKQEFADAILYDLHWLGIFPDATEYQSRRFEVY
DAAVERLKAAGVLYACYETPEELDLRRKVRRTRGLPPVYGREALALTHEQVAEYQADGRRPHWRFLLPNFTGDPLQPERT
EVHWKDLVRGEETVDLASLSDPVLVREDGTYLYTLPSVVDDIEMGVSHVIRGDDHVTNTGVQIALFKALGTEPPVFGHHN
LLTTVSGEGLSKRTGALSIESLREDGIEPMAVASLAVLVGTSENVAAAHDLTELAGHFDPAATSKSSAKFDPDELFVLNR
VLMHHMPFDEARDRLMVLGISGEQAEPFWLAVRGNLDRLADAVGWWRILREGPQDRPEFSDDDRDFLGQAFEVLPEEPWN
GTVWKDWTGKIREATGRKGKGLFMPLRLALTGLPSGPELADLLPLMGREGTLARRP

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=494, Percent_Identity=30.7692307692308, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI62241042, Length=109, Percent_Identity=39.4495412844037, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI4826960, Length=103, Percent_Identity=38.8349514563107, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1788743, Length=483, Percent_Identity=31.8840579710145, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI226510925, Length=285, Percent_Identity=28.4210526315789, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1786895, Length=104, Percent_Identity=34.6153846153846, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71993394, Length=475, Percent_Identity=29.8947368421053, Blast_Score=172, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6324540, Length=496, Percent_Identity=29.8387096774194, Blast_Score=167, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6321192, Length=231, Percent_Identity=33.7662337662338, Blast_Score=90, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6324742, Length=127, Percent_Identity=32.2834645669291, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24665321, Length=486, Percent_Identity=30.4526748971193, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24649466, Length=109, Percent_Identity=38.5321100917431, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE2_RHILO (Q98LZ1)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_102536.1
- ProteinModelPortal:   Q98LZ1
- SMR:   Q98LZ1
- GeneID:   1225199
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mll0813
- NMPDR:   fig|266835.1.peg.641
- HOGENOM:   HBG628189
- OMA:   PHWRFKL
- ProtClustDB:   PRK12558
- BRENDA:   6.1.1.17
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 51184; Mature: 51053

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 253-253

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTVRFAPSPTGRIHIGNARTALFNWLFALNNKGRFIQRFDDTDIGRSKQEFADAILYD
CEEEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHH
LHWLGIFPDATEYQSRRFEVYDAAVERLKAAGVLYACYETPEELDLRRKVRRTRGLPPVY
HHHEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHCCCCCCC
GREALALTHEQVAEYQADGRRPHWRFLLPNFTGDPLQPERTEVHWKDLVRGEETVDLASL
CCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCEEHHCC
SDPVLVREDGTYLYTLPSVVDDIEMGVSHVIRGDDHVTNTGVQIALFKALGTEPPVFGHH
CCCEEEEECCCEEEECHHHHHHHHHHHHHHHCCCCCEECCCEEEEEEEHHCCCCCCCCCC
NLLTTVSGEGLSKRTGALSIESLREDGIEPMAVASLAVLVGTSENVAAAHDLTELAGHFD
CEEEEECCCCCCCCCCCEEHHHHHHCCCCHHHHHHHHHEECCCCCCHHHHHHHHHHHCCC
PAATSKSSAKFDPDELFVLNRVLMHHMPFDEARDRLMVLGISGEQAEPFWLAVRGNLDRL
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHCCCEEEEEEECCCCCCCEEEEEECCHHHH
ADAVGWWRILREGPQDRPEFSDDDRDFLGQAFEVLPEEPWNGTVWKDWTGKIREATGRKG
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHCCCCC
KGLFMPLRLALTGLPSGPELADLLPLMGREGTLARRP
CCEEEEHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TVTVRFAPSPTGRIHIGNARTALFNWLFALNNKGRFIQRFDDTDIGRSKQEFADAILYD
EEEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHH
LHWLGIFPDATEYQSRRFEVYDAAVERLKAAGVLYACYETPEELDLRRKVRRTRGLPPVY
HHHEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHCCCCCCC
GREALALTHEQVAEYQADGRRPHWRFLLPNFTGDPLQPERTEVHWKDLVRGEETVDLASL
CCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCEEHHCC
SDPVLVREDGTYLYTLPSVVDDIEMGVSHVIRGDDHVTNTGVQIALFKALGTEPPVFGHH
CCCEEEEECCCEEEECHHHHHHHHHHHHHHHCCCCCEECCCEEEEEEEHHCCCCCCCCCC
NLLTTVSGEGLSKRTGALSIESLREDGIEPMAVASLAVLVGTSENVAAAHDLTELAGHFD
CEEEEECCCCCCCCCCCEEHHHHHHCCCCHHHHHHHHHEECCCCCCHHHHHHHHHHHCCC
PAATSKSSAKFDPDELFVLNRVLMHHMPFDEARDRLMVLGISGEQAEPFWLAVRGNLDRL
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHCCCEEEEEEECCCCCCCEEEEEECCHHHH
ADAVGWWRILREGPQDRPEFSDDDRDFLGQAFEVLPEEPWNGTVWKDWTGKIREATGRKG
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHCCCCC
KGLFMPLRLALTGLPSGPELADLLPLMGREGTLARRP
CCEEEEHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968