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The map label for this gene is nepI [H]

Identifier: 13470943

GI number: 13470943

Start: 612332

End: 613540

Strand: Reverse

Name: nepI [H]

Synonym: mll0781

Alternate gene names: 13470943

Gene position: 613540-612332 (Counterclockwise)

Preceding gene: 13470944

Following gene: 13470942

Centisome position: 8.72

GC content: 62.61

Gene sequence:

>1209_bases
GTGACAGACACGACATTACAGCTTGAAGATGCGGCGATAGACGTTGATGCGGCTGCGCCGGCCACGTGGGACGCAACCAT
CTGGTTCGCGGTCATTTCATTGGCCGCCACCAGCTTTGCCCTGGTGTCAGCCGAATTTCTGCCGGCAGGTCTGCTGACGC
CCCTGGCGCGCGATCTCGGCATCAGCGAAGGAACGGCCGGGCAGGTCGTCACCGCCACAGCTTCTGTCGGCGCCGTGACG
GCCATGTTGAGCAATGTTCTCATCGGCCGATTAAACCGCAAGGCAGTGCTGGTTGGTCTCAGCGCCTTGGCCGTTGGTTC
CAACATTCTTGCGGCGCTGGCGACCGATTTCTGGCTGTTGTTGCTGGGTCGGGCCGGACTGGGGATCGCTCTCAGCGCTT
TCTGGGCGCTTTCGGTCGCCGTCGTGGCGAGGCTGGTTGGCGCCAATGCGACTGGGCGGGGCATGGCTATCGTCACCCTC
GGCGTCTCGCTCGCAACCATAGCCGCACCGTCGATGGGCGCGTTGATCAGCGACTGGCTGGGTTGGCGCAGCGCCATGGC
CATGACAGCGGGGTTGGCCTTGCTTGCCATGCTGCTGCAGTTGCTTAGCCTGCCGTCCTTGCCTGCAACGGCAAGCAATA
GTCTTGCAGACGTCCTTCGGCTGACACGGCGGCGTGGCATTCAACTTGGTATGCTTGCTATCCTTTTGTTGATGACGGGG
CATTTTGCCGGCTCGGTCTATGTGCGCCCCTTCCTCGAACATGTGACGCTCCTTGCAACCGGGCCAATTGCCCTGGCGTT
GCTCGGATTTGGCATCGCCGCAGTGATCGGCAATATTGCTGGTGGCCGCATGGCCGACGCCAATATCCACGTGGCTCTCG
TGGTTAACGCCGCATTGATGGCGTTTGCGGCGCTGGCATTGGTGCTCTGGGGCGTGCACATTGGCGTTGCCTTTGGCTTC
GCTACGCTGTGGGGCTTCGCCTTCGGCATGGCGCCGGTGGTGCTGCCGACAAATTTGTCTCGCGCAGCGCCGGACGCGCT
GGAAGCAGCGGGCAGCCTGATGGTCGCCTCTTTTCAGGTCGCCATCACCATCGGCGCGGTTGTCGGCGGCTATGCTGTGG
ACACCTATGGCCCTACGGCGCCTTTGACCGTGACTGCTATCCTTGCAGCATCGACAGCCGTCTTGGCGCTGCTACAGCCG
CGCGGCTAG

Upstream 100 bases:

>100_bases
GCACAATTCCCGGACGAATGGACATGAATTTCGGATTGTGAGACACATATCATCCGAAGAAACCCGTCTATGAAGATCTC
CAAACTTTGGAGATACTTCC

Downstream 100 bases:

>100_bases
CTTTGCCGCCGCTGCCACAGCAGCTCGAATCTGGCCCGGACAATATTGCAGGCGCGTTACACAAGTTTACGCAATGCGCG
CGTTGCTTGTTGCCACAATC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MTDTTLQLEDAAIDVDAAAPATWDATIWFAVISLAATSFALVSAEFLPAGLLTPLARDLGISEGTAGQVVTATASVGAVT
AMLSNVLIGRLNRKAVLVGLSALAVGSNILAALATDFWLLLLGRAGLGIALSAFWALSVAVVARLVGANATGRGMAIVTL
GVSLATIAAPSMGALISDWLGWRSAMAMTAGLALLAMLLQLLSLPSLPATASNSLADVLRLTRRRGIQLGMLAILLLMTG
HFAGSVYVRPFLEHVTLLATGPIALALLGFGIAAVIGNIAGGRMADANIHVALVVNAALMAFAALALVLWGVHIGVAFGF
ATLWGFAFGMAPVVLPTNLSRAAPDALEAAGSLMVASFQVAITIGAVVGGYAVDTYGPTAPLTVTAILAASTAVLALLQP
RG

Sequences:

>Translated_402_residues
MTDTTLQLEDAAIDVDAAAPATWDATIWFAVISLAATSFALVSAEFLPAGLLTPLARDLGISEGTAGQVVTATASVGAVT
AMLSNVLIGRLNRKAVLVGLSALAVGSNILAALATDFWLLLLGRAGLGIALSAFWALSVAVVARLVGANATGRGMAIVTL
GVSLATIAAPSMGALISDWLGWRSAMAMTAGLALLAMLLQLLSLPSLPATASNSLADVLRLTRRRGIQLGMLAILLLMTG
HFAGSVYVRPFLEHVTLLATGPIALALLGFGIAAVIGNIAGGRMADANIHVALVVNAALMAFAALALVLWGVHIGVAFGF
ATLWGFAFGMAPVVLPTNLSRAAPDALEAAGSLMVASFQVAITIGAVVGGYAVDTYGPTAPLTVTAILAASTAVLALLQP
RG
>Mature_401_residues
TDTTLQLEDAAIDVDAAAPATWDATIWFAVISLAATSFALVSAEFLPAGLLTPLARDLGISEGTAGQVVTATASVGAVTA
MLSNVLIGRLNRKAVLVGLSALAVGSNILAALATDFWLLLLGRAGLGIALSAFWALSVAVVARLVGANATGRGMAIVTLG
VSLATIAAPSMGALISDWLGWRSAMAMTAGLALLAMLLQLLSLPSLPATASNSLADVLRLTRRRGIQLGMLAILLLMTGH
FAGSVYVRPFLEHVTLLATGPIALALLGFGIAAVIGNIAGGRMADANIHVALVVNAALMAFAALALVLWGVHIGVAFGFA
TLWGFAFGMAPVVLPTNLSRAAPDALEAAGSLMVASFQVAITIGAVVGGYAVDTYGPTAPLTVTAILAASTAVLALLQPR
G

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and especially inosine. Involved in the resistance to 6-mercaptopurine [H]

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. NepI (TC 2.A.1.2.26) subfamily [H]

Homologues:

Organism=Escherichia coli, GI145693198, Length=368, Percent_Identity=38.0434782608696, Blast_Score=227, Evalue=8e-61,
Organism=Escherichia coli, GI1787808, Length=347, Percent_Identity=25.3602305475504, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787947, Length=284, Percent_Identity=28.5211267605634, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1786595, Length=255, Percent_Identity=25.0980392156863, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 40580; Mature: 40448

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTTLQLEDAAIDVDAAAPATWDATIWFAVISLAATSFALVSAEFLPAGLLTPLARDLG
CCCCEEEEECCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
ISEGTAGQVVTATASVGAVTAMLSNVLIGRLNRKAVLVGLSALAVGSNILAALATDFWLL
CCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHH
LLGRAGLGIALSAFWALSVAVVARLVGANATGRGMAIVTLGVSLATIAAPSMGALISDWL
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHCCCCHHHHHHHHH
GWRSAMAMTAGLALLAMLLQLLSLPSLPATASNSLADVLRLTRRRGIQLGMLAILLLMTG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHC
HFAGSVYVRPFLEHVTLLATGPIALALLGFGIAAVIGNIAGGRMADANIHVALVVNAALM
CCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEECCCEEEHHHHHHHHH
AFAALALVLWGVHIGVAFGFATLWGFAFGMAPVVLPTNLSRAAPDALEAAGSLMVASFQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHCCHHHHHHHH
AITIGAVVGGYAVDTYGPTAPLTVTAILAASTAVLALLQPRG
HHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TDTTLQLEDAAIDVDAAAPATWDATIWFAVISLAATSFALVSAEFLPAGLLTPLARDLG
CCCEEEEECCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCC
ISEGTAGQVVTATASVGAVTAMLSNVLIGRLNRKAVLVGLSALAVGSNILAALATDFWLL
CCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHH
LLGRAGLGIALSAFWALSVAVVARLVGANATGRGMAIVTLGVSLATIAAPSMGALISDWL
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHCCCCHHHHHHHHH
GWRSAMAMTAGLALLAMLLQLLSLPSLPATASNSLADVLRLTRRRGIQLGMLAILLLMTG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHC
HFAGSVYVRPFLEHVTLLATGPIALALLGFGIAAVIGNIAGGRMADANIHVALVVNAALM
CCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCEECCCEEEHHHHHHHHH
AFAALALVLWGVHIGVAFGFATLWGFAFGMAPVVLPTNLSRAAPDALEAAGSLMVASFQV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHCCHHHHHHHH
AITIGAVVGGYAVDTYGPTAPLTVTAILAASTAVLALLQPRG
HHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA