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The map label for this gene is pyrB

Identifier: 13470872

GI number: 13470872

Start: 536277

End: 537257

Strand: Direct

Name: pyrB

Synonym: mlr0686

Alternate gene names: 13470872

Gene position: 536277-537257 (Clockwise)

Preceding gene: 13470869

Following gene: 13470873

Centisome position: 7.62

GC content: 64.93

Gene sequence:

>981_bases
ATGACCGACGCTTCGTCCCTGCCACTCTACCCTCACCGCCATCTTCTGGGCATCAGCGATCTTTCGCCCGCCGACATCGA
GCTGTTGCTCGACCGGGCGGATCGGGCCGTCGCGATTTCGCGGCAGTCCGAAAAGAAGACCTCGACGCTGCGCGGCCGCA
CCCAGATCAATCTCTTCTACGAGGCCTCGACCCGCACGCAGTCCTCTTTCGAGCTGGCCGGAAAGCGGCTCGGCGCCGAT
GTCATGAACATGTCGGTGGCAAGTTCTTCCGTGAAGAAGGGCGAAACGCTCATCGACACGGCGATGACGCTGAACGCCAT
GCGGCCCGATATATTGATCATCCGCCATCAGTCGGCGGGTGCTGCCGCCCTGCTGGCGCAGAAAGTCGGCTGCTCGGTCG
TCAATGCCGGCGACGGCGCGCATGAACACCCGACCCAGGCGCTGCTCGACGCGCTAACCATCCGCCGCGCCAAGGGGCCG
CTGTCGAAGCTGATCGTGGCGATCTGCGGCGACATCCTGCATTCGCGCGTGGCGCGCTCCAACATCATGCTGCTCAACGC
GCTTGGCGCGCAGGTGCGCGTGGTGGCGCCTTCGACGCTGCTACCGTCCGGCATAGAGAAGATGGGCGTCATCGTCACGC
GCTCGATGGCCGAAGGCCTCAAGGACGCCGACGTGGTGATGATGCTGCGCCTGCAGCGCGAGCGCATGGAAGGCGCCTTC
GTGCCTTCGGTGCGCGAATATTTCCGCTATTTCGGCCTCGATGCCGAGAAGCTGAAGGCCGCCAAGGGTGACGCGCTGGT
GATGCATCCCGGCCCGATGAACCGCGGCGTCGAAATCGCCTCGGAAATCGCCGACGGCCCGCAAAGCGTGATCCAGGAGC
AGGTGGAGATGGGCGTCGCCGTGCGCATGGCGGTGATGGAAGCGCTGCTCGACCCCCGTCGTAATCAAGAGGGCCGCAAA
CAGGAGGGTCGCGGCGCATGA

Upstream 100 bases:

>100_bases
AGCGTTGCCGCGCGCGCGACGAAAATACCGGGGAGAACAGCGCCAATCCAAAGCTGGCGCTTGCGGGCGCAAAATCCGCA
CCCTATAGCAGCCCCTTGAG

Downstream 100 bases:

>100_bases
GCACGACAGTCTTTGAACGCGCCCGCATCGTCGACCCTTCGCGCGGCATCGACGAAGTCGGCTCCGTCATCGTCGAGGGC
CGCAAGATCGTTGCCGCCGG

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MTDASSLPLYPHRHLLGISDLSPADIELLLDRADRAVAISRQSEKKTSTLRGRTQINLFYEASTRTQSSFELAGKRLGAD
VMNMSVASSSVKKGETLIDTAMTLNAMRPDILIIRHQSAGAAALLAQKVGCSVVNAGDGAHEHPTQALLDALTIRRAKGP
LSKLIVAICGDILHSRVARSNIMLLNALGAQVRVVAPSTLLPSGIEKMGVIVTRSMAEGLKDADVVMMLRLQRERMEGAF
VPSVREYFRYFGLDAEKLKAAKGDALVMHPGPMNRGVEIASEIADGPQSVIQEQVEMGVAVRMAVMEALLDPRRNQEGRK
QEGRGA

Sequences:

>Translated_326_residues
MTDASSLPLYPHRHLLGISDLSPADIELLLDRADRAVAISRQSEKKTSTLRGRTQINLFYEASTRTQSSFELAGKRLGAD
VMNMSVASSSVKKGETLIDTAMTLNAMRPDILIIRHQSAGAAALLAQKVGCSVVNAGDGAHEHPTQALLDALTIRRAKGP
LSKLIVAICGDILHSRVARSNIMLLNALGAQVRVVAPSTLLPSGIEKMGVIVTRSMAEGLKDADVVMMLRLQRERMEGAF
VPSVREYFRYFGLDAEKLKAAKGDALVMHPGPMNRGVEIASEIADGPQSVIQEQVEMGVAVRMAVMEALLDPRRNQEGRK
QEGRGA
>Mature_325_residues
TDASSLPLYPHRHLLGISDLSPADIELLLDRADRAVAISRQSEKKTSTLRGRTQINLFYEASTRTQSSFELAGKRLGADV
MNMSVASSSVKKGETLIDTAMTLNAMRPDILIIRHQSAGAAALLAQKVGCSVVNAGDGAHEHPTQALLDALTIRRAKGPL
SKLIVAICGDILHSRVARSNIMLLNALGAQVRVVAPSTLLPSGIEKMGVIVTRSMAEGLKDADVVMMLRLQRERMEGAFV
PSVREYFRYFGLDAEKLKAAKGDALVMHPGPMNRGVEIASEIADGPQSVIQEQVEMGVAVRMAVMEALLDPRRNQEGRKQ
EGRGA

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=310, Percent_Identity=35.4838709677419, Blast_Score=171, Evalue=6e-43,
Organism=Escherichia coli, GI2367364, Length=315, Percent_Identity=34.2857142857143, Blast_Score=150, Evalue=9e-38,
Organism=Escherichia coli, GI1790703, Length=232, Percent_Identity=28.8793103448276, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1786469, Length=229, Percent_Identity=29.6943231441048, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=320, Percent_Identity=35, Blast_Score=143, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6322331, Length=306, Percent_Identity=33.3333333333333, Blast_Score=141, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24642586, Length=267, Percent_Identity=37.8277153558052, Blast_Score=155, Evalue=4e-38,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_RHILO (Q98M86)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_102441.1
- ProteinModelPortal:   Q98M86
- SMR:   Q98M86
- GeneID:   1225104
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr0686
- NMPDR:   fig|266835.1.peg.546
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- BRENDA:   2.1.3.2
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 35213; Mature: 35082

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDASSLPLYPHRHLLGISDLSPADIELLLDRADRAVAISRQSEKKTSTLRGRTQINLFY
CCCCCCCCCCCCHHEECCCCCCCHHHHHHHHCCCCEEEECCHHHHHHHHHCCCEEEEEEE
EASTRTQSSFELAGKRLGADVMNMSVASSSVKKGETLIDTAMTLNAMRPDILIIRHQSAG
ECCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEECCCC
AAALLAQKVGCSVVNAGDGAHEHPTQALLDALTIRRAKGPLSKLIVAICGDILHSRVARS
HHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
NIMLLNALGAQVRVVAPSTLLPSGIEKMGVIVTRSMAEGLKDADVVMMLRLQRERMEGAF
CEEEEEECCCEEEEEECCHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
VPSVREYFRYFGLDAEKLKAAKGDALVMHPGPMNRGVEIASEIADGPQSVIQEQVEMGVA
CHHHHHHHHHHCCCHHHHHHCCCCEEEECCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHH
VRMAVMEALLDPRRNQEGRKQEGRGA
HHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDASSLPLYPHRHLLGISDLSPADIELLLDRADRAVAISRQSEKKTSTLRGRTQINLFY
CCCCCCCCCCCHHEECCCCCCCHHHHHHHHCCCCEEEECCHHHHHHHHHCCCEEEEEEE
EASTRTQSSFELAGKRLGADVMNMSVASSSVKKGETLIDTAMTLNAMRPDILIIRHQSAG
ECCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEECCCC
AAALLAQKVGCSVVNAGDGAHEHPTQALLDALTIRRAKGPLSKLIVAICGDILHSRVARS
HHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
NIMLLNALGAQVRVVAPSTLLPSGIEKMGVIVTRSMAEGLKDADVVMMLRLQRERMEGAF
CEEEEEECCCEEEEEECCHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
VPSVREYFRYFGLDAEKLKAAKGDALVMHPGPMNRGVEIASEIADGPQSVIQEQVEMGVA
CHHHHHHHHHHCCCHHHHHHCCCCEEEECCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHH
VRMAVMEALLDPRRNQEGRKQEGRGA
HHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968