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The map label for this gene is bclA [H]

Identifier: 13470819

GI number: 13470819

Start: 484176

End: 485801

Strand: Direct

Name: bclA [H]

Synonym: mlr0619

Alternate gene names: 13470819

Gene position: 484176-485801 (Clockwise)

Preceding gene: 13470817

Following gene: 13470820

Centisome position: 6.88

GC content: 64.02

Gene sequence:

>1626_bases
ATGCTTGGGCCTTCAGCGCATATCGACACGTTCACGCGCGATCATCTGCCGCCGCCGGATCAATGGCCGGATTTCCTGCT
CGATGGCTTCGACTATCCTGAACATCTCAACGTCGGCGTCGAATTGACCGACAGGCTGGTCGAGAAGGGTCTTGGCGACC
ACACGGCCTTGATCGGCAATGGCCGCCGCCGCACCTACAAGGAACTGTCCGACTGGACGAACAGGCTGGCCCACGCGCTG
GTCGAGAATTACGACGTCAAGCCGGGCAACCGGGTGCTGATCCGCTCCGCCAACAACCCCGCAATGGTCGCCTGCTGGCT
TGCCGCCACCAAAGTCGGCGCGGTGGTCGTCAACACCATGCCGATGCTGCGCACCGGCGAGCTCACCAAGATCGTCGACA
AGGCGGAAATAACCATCGCGCTCTGCGACACCAGGCTGATGGACGAGATGACCGCCTGCGCCAAGGACAGCACCTTCCTG
AAGCAGGTCATCGGCTTCGACGGCACCGCCAACCATGATGCCGAGCTTGATCGCGCCGCGCTCGACAAGCCGGTCACCTT
CACCGCCGTCAACACCGGCCGCGACGACGTCGCCTTGCTCGGCTTCACCTCGGGCACGACGGGCGTGCCGAAGGCGACCA
TGCACTTCCACCGCGATCTCCTGATCATCGCCGATGCCTATGCCCGGGAAATCCTCCAGGTCACGCCGGACGACATCTTC
GTCGGCTCGCCGCCGCTCGCCTTCACCTTCGGCCTTGGCGGCCTCGCCATCTTCCCCCTGCGCTTCGGTGCAGCGGCAAC
ATTGCTGGAACAAGCAACGCCGCCCAACATGATCCACATCATCGAGACCTACAAAGCGACCATCTCCTTCACCGCGCCGA
CCGCCTATCGCGCCATGCTGAAGGCCATGGATGAAGGCGCTGACCTGTCATCGCTGCGCGTCGCCGTTTCGGCCGGCGAG
ACATTGCCGGCTCCGGTTTTCGAGGAATGGACCGAGAAGACCGGCAAGCCGATCCTCGACGGCATCGGCGCCACCGAAAT
GCTGCACATCTTCATCTCCAACCGCTTTGACGACAGGAAGCCAGCGTCGACCGGCAAGCCTGTGGGCGGCTACGAAGCGC
GCATCGTCGACGACGCGATGCGCGAGGTGCCGCGCGGCGAGACCGGGCGGCTGGCGGTGCGCGGCCCGACCGGCTGCCGC
TACATGGCCGACGACAGGCAGAAGGAGTATGTGCGCGACGGCTGGAACCTGACCGGCGACACTTTCACCCAGGACGAGGA
CGGTTTCTTCCATTTCGCCGCGCGTTCCGACGATATGATCGTCAGCGCCGGCTACAACATTGCCGGGCCTGAGGTCGAGG
CGGCACTGCTGTCGCATCCCGATGTCGCCGAATGCGCGGTCATCGGCGCAGAGGATGGCGAACGCGGCCAGATCGTCGAG
GCGCATGTCGTGCTGGTGCAAGGCGTGGCGCCCGATGCGTTGACCGTCAAGCGCCTGCAGGACCACGTCAAGGCAATGAT
CGCGCCCTACAAATATCCGCGCTCGATCAAATTCATCGCCGCCCTGCCCAAGACCCAGACCGGCAAGATCCAGCGCTTCC
GGCTGCGCACGGAGAAAATCAATTGA

Upstream 100 bases:

>100_bases
GGCCGCACAAAGCCTCTCGCGAAAAGACGGGAACTTCAGATCCACCACCTCGGACAGGTGCTTCGCGCACTGGCCAGGCA
GCAAATGGGATGGCGGCGGC

Downstream 100 bases:

>100_bases
GCGAGCCCTACGATCCGGCGTCCGAAGGCGCACAGATGTCGTTCAACGAGCGCATGTCCTACAGCGACTATCTGCACCTG
GAAAAAGTGCTGGAGGCACA

Product: acid-CoA ligase

Products: NA

Alternate protein names: Benzoyl-CoA synthetase [H]

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MLGPSAHIDTFTRDHLPPPDQWPDFLLDGFDYPEHLNVGVELTDRLVEKGLGDHTALIGNGRRRTYKELSDWTNRLAHAL
VENYDVKPGNRVLIRSANNPAMVACWLAATKVGAVVVNTMPMLRTGELTKIVDKAEITIALCDTRLMDEMTACAKDSTFL
KQVIGFDGTANHDAELDRAALDKPVTFTAVNTGRDDVALLGFTSGTTGVPKATMHFHRDLLIIADAYAREILQVTPDDIF
VGSPPLAFTFGLGGLAIFPLRFGAAATLLEQATPPNMIHIIETYKATISFTAPTAYRAMLKAMDEGADLSSLRVAVSAGE
TLPAPVFEEWTEKTGKPILDGIGATEMLHIFISNRFDDRKPASTGKPVGGYEARIVDDAMREVPRGETGRLAVRGPTGCR
YMADDRQKEYVRDGWNLTGDTFTQDEDGFFHFAARSDDMIVSAGYNIAGPEVEAALLSHPDVAECAVIGAEDGERGQIVE
AHVVLVQGVAPDALTVKRLQDHVKAMIAPYKYPRSIKFIAALPKTQTGKIQRFRLRTEKIN

Sequences:

>Translated_541_residues
MLGPSAHIDTFTRDHLPPPDQWPDFLLDGFDYPEHLNVGVELTDRLVEKGLGDHTALIGNGRRRTYKELSDWTNRLAHAL
VENYDVKPGNRVLIRSANNPAMVACWLAATKVGAVVVNTMPMLRTGELTKIVDKAEITIALCDTRLMDEMTACAKDSTFL
KQVIGFDGTANHDAELDRAALDKPVTFTAVNTGRDDVALLGFTSGTTGVPKATMHFHRDLLIIADAYAREILQVTPDDIF
VGSPPLAFTFGLGGLAIFPLRFGAAATLLEQATPPNMIHIIETYKATISFTAPTAYRAMLKAMDEGADLSSLRVAVSAGE
TLPAPVFEEWTEKTGKPILDGIGATEMLHIFISNRFDDRKPASTGKPVGGYEARIVDDAMREVPRGETGRLAVRGPTGCR
YMADDRQKEYVRDGWNLTGDTFTQDEDGFFHFAARSDDMIVSAGYNIAGPEVEAALLSHPDVAECAVIGAEDGERGQIVE
AHVVLVQGVAPDALTVKRLQDHVKAMIAPYKYPRSIKFIAALPKTQTGKIQRFRLRTEKIN
>Mature_541_residues
MLGPSAHIDTFTRDHLPPPDQWPDFLLDGFDYPEHLNVGVELTDRLVEKGLGDHTALIGNGRRRTYKELSDWTNRLAHAL
VENYDVKPGNRVLIRSANNPAMVACWLAATKVGAVVVNTMPMLRTGELTKIVDKAEITIALCDTRLMDEMTACAKDSTFL
KQVIGFDGTANHDAELDRAALDKPVTFTAVNTGRDDVALLGFTSGTTGVPKATMHFHRDLLIIADAYAREILQVTPDDIF
VGSPPLAFTFGLGGLAIFPLRFGAAATLLEQATPPNMIHIIETYKATISFTAPTAYRAMLKAMDEGADLSSLRVAVSAGE
TLPAPVFEEWTEKTGKPILDGIGATEMLHIFISNRFDDRKPASTGKPVGGYEARIVDDAMREVPRGETGRLAVRGPTGCR
YMADDRQKEYVRDGWNLTGDTFTQDEDGFFHFAARSDDMIVSAGYNIAGPEVEAALLSHPDVAECAVIGAEDGERGQIVE
AHVVLVQGVAPDALTVKRLQDHVKAMIAPYKYPRSIKFIAALPKTQTGKIQRFRLRTEKIN

Specific function: Catalyzes the ligation of benzoate and CoA to form benzoyl-CoA at the expense of ATP. The enzyme also ligates 2- aminobenzoate and CoA. The enzyme shows activity toward a number of benzoate derivates [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. Benzoate-CoA ligase subfamily [H]

Homologues:

Organism=Homo sapiens, GI42544132, Length=536, Percent_Identity=29.8507462686567, Blast_Score=215, Evalue=9e-56,
Organism=Homo sapiens, GI122937307, Length=548, Percent_Identity=29.5620437956204, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI58082049, Length=492, Percent_Identity=31.3008130081301, Blast_Score=195, Evalue=7e-50,
Organism=Homo sapiens, GI157311624, Length=492, Percent_Identity=30.6910569105691, Blast_Score=192, Evalue=5e-49,
Organism=Homo sapiens, GI157311622, Length=492, Percent_Identity=30.6910569105691, Blast_Score=192, Evalue=5e-49,
Organism=Homo sapiens, GI38505220, Length=493, Percent_Identity=28.8032454361055, Blast_Score=187, Evalue=3e-47,
Organism=Homo sapiens, GI115511026, Length=538, Percent_Identity=26.7657992565056, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI187761345, Length=528, Percent_Identity=25.5681818181818, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI187761343, Length=528, Percent_Identity=25.5681818181818, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI28416953, Length=550, Percent_Identity=28.3636363636364, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI13375727, Length=549, Percent_Identity=25.5009107468124, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI156151445, Length=526, Percent_Identity=25.4752851711027, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI8923896, Length=572, Percent_Identity=23.9510489510489, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI253970489, Length=582, Percent_Identity=23.5395189003436, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI195947378, Length=453, Percent_Identity=23.3995584988962, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI42544134, Length=367, Percent_Identity=23.9782016348774, Blast_Score=101, Evalue=2e-21,
Organism=Escherichia coli, GI145693145, Length=506, Percent_Identity=30.0395256916996, Blast_Score=188, Evalue=7e-49,
Organism=Escherichia coli, GI1788107, Length=520, Percent_Identity=28.6538461538462, Blast_Score=178, Evalue=7e-46,
Organism=Escherichia coli, GI1790505, Length=554, Percent_Identity=26.8953068592058, Blast_Score=162, Evalue=7e-41,
Organism=Escherichia coli, GI221142682, Length=506, Percent_Identity=26.6798418972332, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1786810, Length=516, Percent_Identity=25.968992248062, Blast_Score=135, Evalue=9e-33,
Organism=Escherichia coli, GI1786529, Length=583, Percent_Identity=27.2727272727273, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1789201, Length=478, Percent_Identity=25.7322175732218, Blast_Score=96, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17558820, Length=493, Percent_Identity=26.7748478701826, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI32563687, Length=483, Percent_Identity=26.5010351966874, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17538037, Length=504, Percent_Identity=26.5873015873016, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17557194, Length=502, Percent_Identity=26.4940239043825, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI71996755, Length=532, Percent_Identity=26.1278195488722, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17559526, Length=364, Percent_Identity=28.8461538461538, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI71994690, Length=530, Percent_Identity=27.1698113207547, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI71994694, Length=530, Percent_Identity=27.1698113207547, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI71994703, Length=530, Percent_Identity=27.1698113207547, Blast_Score=118, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17531443, Length=491, Percent_Identity=24.2362525458248, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71982997, Length=561, Percent_Identity=25.668449197861, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71983001, Length=561, Percent_Identity=25.668449197861, Blast_Score=110, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17560308, Length=524, Percent_Identity=26.5267175572519, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17560140, Length=498, Percent_Identity=23.4939759036145, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71985884, Length=389, Percent_Identity=25.706940874036, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17507435, Length=493, Percent_Identity=23.9350912778905, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319264, Length=528, Percent_Identity=28.5984848484849, Blast_Score=158, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6323182, Length=528, Percent_Identity=28.5984848484849, Blast_Score=143, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6319699, Length=348, Percent_Identity=29.3103448275862, Blast_Score=131, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24667955, Length=564, Percent_Identity=27.1276595744681, Blast_Score=139, Evalue=6e-33,
Organism=Drosophila melanogaster, GI62472339, Length=532, Percent_Identity=26.6917293233083, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24581924, Length=527, Percent_Identity=26.1859582542694, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI21355181, Length=506, Percent_Identity=26.6798418972332, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24667959, Length=367, Percent_Identity=28.3378746594005, Blast_Score=122, Evalue=7e-28,
Organism=Drosophila melanogaster, GI21355225, Length=554, Percent_Identity=24.9097472924188, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI18859661, Length=356, Percent_Identity=28.0898876404494, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648260, Length=356, Percent_Identity=27.247191011236, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24653035, Length=495, Percent_Identity=24.040404040404, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI161076582, Length=509, Percent_Identity=23.7721021611002, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21356441, Length=354, Percent_Identity=25.7062146892655, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24648257, Length=352, Percent_Identity=26.4204545454545, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356947, Length=492, Percent_Identity=24.1869918699187, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19922652, Length=505, Percent_Identity=23.3663366336634, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24648253, Length=350, Percent_Identity=25.7142857142857, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648255, Length=350, Percent_Identity=26, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21358303, Length=313, Percent_Identity=25.5591054313099, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI20130357, Length=352, Percent_Identity=22.7272727272727, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011957 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.25 [H]

Molecular weight: Translated: 59148; Mature: 59148

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGPSAHIDTFTRDHLPPPDQWPDFLLDGFDYPEHLNVGVELTDRLVEKGLGDHTALIGN
CCCCCCCCCCCHHCCCCCCCCCHHHHHCCCCCHHHCCCCHHHHHHHHHCCCCCCEEEECC
GRRRTYKELSDWTNRLAHALVENYDVKPGNRVLIRSANNPAMVACWLAATKVGAVVVNTM
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEEEHHHHHHCEEEECCC
PMLRTGELTKIVDKAEITIALCDTRLMDEMTACAKDSTFLKQVIGFDGTANHDAELDRAA
CCCCCCCHHHHHCCCEEEEEEECHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHH
LDKPVTFTAVNTGRDDVALLGFTSGTTGVPKATMHFHRDLLIIADAYAREILQVTPDDIF
CCCCEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHCCCEEEEECHHHHHHHHCCCCCEE
VGSPPLAFTFGLGGLAIFPLRFGAAATLLEQATPPNMIHIIETYKATISFTAPTAYRAML
ECCCCCEEEECCCCCEEHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEEECCHHHHHHH
KAMDEGADLSSLRVAVSAGETLPAPVFEEWTEKTGKPILDGIGATEMLHIFISNRFDDRK
HHHHCCCCHHHEEEEEECCCCCCCHHHHHHHHHCCCHHHHCCCHHHHHHHHHHCCCCCCC
PASTGKPVGGYEARIVDDAMREVPRGETGRLAVRGPTGCRYMADDRQKEYVRDGWNLTGD
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHCCCCCCCC
TFTQDEDGFFHFAARSDDMIVSAGYNIAGPEVEAALLSHPDVAECAVIGAEDGERGQIVE
CCCCCCCCEEEEEECCCCEEEECCCCCCCCCHHHHHHCCCCHHHEEEEECCCCCCCCEEE
AHVVLVQGVAPDALTVKRLQDHVKAMIAPYKYPRSIKFIAALPKTQTGKIQRFRLRTEKI
EEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCEEEEEEECCCC
N
C
>Mature Secondary Structure
MLGPSAHIDTFTRDHLPPPDQWPDFLLDGFDYPEHLNVGVELTDRLVEKGLGDHTALIGN
CCCCCCCCCCCHHCCCCCCCCCHHHHHCCCCCHHHCCCCHHHHHHHHHCCCCCCEEEECC
GRRRTYKELSDWTNRLAHALVENYDVKPGNRVLIRSANNPAMVACWLAATKVGAVVVNTM
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEEEHHHHHHCEEEECCC
PMLRTGELTKIVDKAEITIALCDTRLMDEMTACAKDSTFLKQVIGFDGTANHDAELDRAA
CCCCCCCHHHHHCCCEEEEEEECHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHH
LDKPVTFTAVNTGRDDVALLGFTSGTTGVPKATMHFHRDLLIIADAYAREILQVTPDDIF
CCCCEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHCCCEEEEECHHHHHHHHCCCCCEE
VGSPPLAFTFGLGGLAIFPLRFGAAATLLEQATPPNMIHIIETYKATISFTAPTAYRAML
ECCCCCEEEECCCCCEEHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEEECCHHHHHHH
KAMDEGADLSSLRVAVSAGETLPAPVFEEWTEKTGKPILDGIGATEMLHIFISNRFDDRK
HHHHCCCCHHHEEEEEECCCCCCCHHHHHHHHHCCCHHHHCCCHHHHHHHHHHCCCCCCC
PASTGKPVGGYEARIVDDAMREVPRGETGRLAVRGPTGCRYMADDRQKEYVRDGWNLTGD
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHCCCCCCCC
TFTQDEDGFFHFAARSDDMIVSAGYNIAGPEVEAALLSHPDVAECAVIGAEDGERGQIVE
CCCCCCCCEEEEEECCCCEEEECCCCCCCCCHHHHHHCCCCHHHEEEEECCCCCCCCEEE
AHVVLVQGVAPDALTVKRLQDHVKAMIAPYKYPRSIKFIAALPKTQTGKIQRFRLRTEKI
EEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCEEEEEEECCCC
N
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12897012 [H]