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The map label for this gene is artJ [H]

Identifier: 13470758

GI number: 13470758

Start: 404013

End: 404798

Strand: Direct

Name: artJ [H]

Synonym: mlr0549

Alternate gene names: 13470758

Gene position: 404013-404798 (Clockwise)

Preceding gene: 13470757

Following gene: 13470759

Centisome position: 5.74

GC content: 61.32

Gene sequence:

>786_bases
ATGCGTATTGCACTGCGTATCGCGCTCGCCGCATCGGCTGCGCTGCTGACGCTCGGCGTCGCCCAGGCCCAGGAAAAGAC
CCTGCGGATCGGCACCGAAGGCGCCTATCCCCCGTTCAACAACCTCACCTCCGACGGCAAGCTCGTCGGCTTCGATATCG
ACATCGCCCAGGCGCTTTGCGATCAGATGAAGGTGAAGTGCACCTTCGTCGCCCAGGATTGGGACGGCATCATCCCGGCA
CTTCAGGCCGGCAAGTTCGACGCCATCGTCGCTTCGATGTCGATCACGCCTGAGCGCAAGGAGAAGGTCGACTTCACCCA
CAAATACTACAACACACCCTCGGCTCTCGCAGTGCCGAAAGACAGCACGCTGAAGGGCGTGACCAAGGCAGATCTCGCCG
GCAAGACCATCGGCGTCGCCACGACGACCACCCATTTCAACTATGCCTCGAAGACCTACACCGACAGCACCGTGAAGGGC
TATCCGAGCAGCCCCGAGGAGCAGGCAGATCTGGCCAATGGTCGTCTGGACGCCATCGAGGACGATATCGTCGTGCTCCA
GCAGTGGCTGGACTCGCCTGATGGCGCCTGCTGCAAGATTCTCGGCCAGCCCTCGCCGCAACCGGTCGAGATCTTCGGGC
CGGGCGCCGGCATAGCCGTGCGCAAGGGCGAGACCGATCTGGTCAACAAGCTCAATGAGGCCATCGACGCCATCCGCGCC
AATGGAAAATACAAGGAAATCAACGACAAGTACTTCAAGTTCGACGTCTTCGGCGCCGAGTCCTGA

Upstream 100 bases:

>100_bases
TCTTGGAAAGAATGGGAGTATCGCCTCAGCGGGCGGTCAAAAGCACCGCCCCATAGAGCCGTTTCGGAACGACGGTCGGG
ACCATAAAGAGAGGACTATC

Downstream 100 bases:

>100_bases
TAGACGGATCAAGACGGCGGGGAAATTCCCCGCCGTCTTTCTATTCATCTTGGAACGCCGTGGAGATAAGACCCGTCAAT
GCCAGCCCAAAGCATATGGA

Product: amino acid ABC transporter amino acid-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MRIALRIALAASAALLTLGVAQAQEKTLRIGTEGAYPPFNNLTSDGKLVGFDIDIAQALCDQMKVKCTFVAQDWDGIIPA
LQAGKFDAIVASMSITPERKEKVDFTHKYYNTPSALAVPKDSTLKGVTKADLAGKTIGVATTTTHFNYASKTYTDSTVKG
YPSSPEEQADLANGRLDAIEDDIVVLQQWLDSPDGACCKILGQPSPQPVEIFGPGAGIAVRKGETDLVNKLNEAIDAIRA
NGKYKEINDKYFKFDVFGAES

Sequences:

>Translated_261_residues
MRIALRIALAASAALLTLGVAQAQEKTLRIGTEGAYPPFNNLTSDGKLVGFDIDIAQALCDQMKVKCTFVAQDWDGIIPA
LQAGKFDAIVASMSITPERKEKVDFTHKYYNTPSALAVPKDSTLKGVTKADLAGKTIGVATTTTHFNYASKTYTDSTVKG
YPSSPEEQADLANGRLDAIEDDIVVLQQWLDSPDGACCKILGQPSPQPVEIFGPGAGIAVRKGETDLVNKLNEAIDAIRA
NGKYKEINDKYFKFDVFGAES
>Mature_261_residues
MRIALRIALAASAALLTLGVAQAQEKTLRIGTEGAYPPFNNLTSDGKLVGFDIDIAQALCDQMKVKCTFVAQDWDGIIPA
LQAGKFDAIVASMSITPERKEKVDFTHKYYNTPSALAVPKDSTLKGVTKADLAGKTIGVATTTTHFNYASKTYTDSTVKG
YPSSPEEQADLANGRLDAIEDDIVVLQQWLDSPDGACCKILGQPSPQPVEIFGPGAGIAVRKGETDLVNKLNEAIDAIRA
NGKYKEINDKYFKFDVFGAES

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=247, Percent_Identity=39.2712550607287, Blast_Score=177, Evalue=7e-46,
Organism=Escherichia coli, GI1787088, Length=251, Percent_Identity=37.4501992031873, Blast_Score=167, Evalue=9e-43,
Organism=Escherichia coli, GI1788648, Length=237, Percent_Identity=37.5527426160338, Blast_Score=165, Evalue=3e-42,
Organism=Escherichia coli, GI1788649, Length=236, Percent_Identity=37.7118644067797, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1788228, Length=232, Percent_Identity=31.0344827586207, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1787031, Length=256, Percent_Identity=31.25, Blast_Score=113, Evalue=1e-26,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28053; Mature: 28053

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIALRIALAASAALLTLGVAQAQEKTLRIGTEGAYPPFNNLTSDGKLVGFDIDIAQALC
CEEEEEEEHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCEEEEEEHHHHHHHH
DQMKVKCTFVAQDWDGIIPALQAGKFDAIVASMSITPERKEKVDFTHKYYNTPSALAVPK
HHHEEEEEEEECCCCCCHHHHHCCCCEEEEEEEECCCCHHHHCCHHHHHCCCCCEEECCC
DSTLKGVTKADLAGKTIGVATTTTHFNYASKTYTDSTVKGYPSSPEEQADLANGRLDAIE
CCCCCCCHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHH
DDIVVLQQWLDSPDGACCKILGQPSPQPVEIFGPGAGIAVRKGETDLVNKLNEAIDAIRA
HHHHHHHHHHCCCCCCEEEEECCCCCCCEEEECCCCCEEEECCCHHHHHHHHHHHHHHHC
NGKYKEINDKYFKFDVFGAES
CCCEEECCCEEEEEEEECCCC
>Mature Secondary Structure
MRIALRIALAASAALLTLGVAQAQEKTLRIGTEGAYPPFNNLTSDGKLVGFDIDIAQALC
CEEEEEEEHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCEEEEEEHHHHHHHH
DQMKVKCTFVAQDWDGIIPALQAGKFDAIVASMSITPERKEKVDFTHKYYNTPSALAVPK
HHHEEEEEEEECCCCCCHHHHHCCCCEEEEEEEECCCCHHHHCCHHHHHCCCCCEEECCC
DSTLKGVTKADLAGKTIGVATTTTHFNYASKTYTDSTVKGYPSSPEEQADLANGRLDAIE
CCCCCCCHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHH
DDIVVLQQWLDSPDGACCKILGQPSPQPVEIFGPGAGIAVRKGETDLVNKLNEAIDAIRA
HHHHHHHHHHCCCCCCEEEEECCCCCCCEEEECCCCCEEEECCCHHHHHHHHHHHHHHHC
NGKYKEINDKYFKFDVFGAES
CCCEEECCCEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]