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The map label for this gene is pdhC [H]

Identifier: 13470621

GI number: 13470621

Start: 273228

End: 274589

Strand: Direct

Name: pdhC [H]

Synonym: mlr0385

Alternate gene names: 13470621

Gene position: 273228-274589 (Clockwise)

Preceding gene: 13470620

Following gene: 13470622

Centisome position: 3.88

GC content: 65.27

Gene sequence:

>1362_bases
ATGCCAATCAACATCACCATGCCGGCGCTCTCGCCCACGATGGAAGAGGGCAATCTGTCGAAATGGCTGGTCAAGGAAGG
CGACAAGGTTTCACCCGGAGACGTCATCGCCGAGATCGAGACCGACAAGGCGACGATGGAGGTCGAGGCCGTCGATGAGG
GCACGGTGGCCAAGCTCGTCGTTCCCGCCGGTACCGAAGGCGTCAAGGTCAATGCGCTGATCGCCGTGCTTGCGGCCGAG
GGCGAGGATGCGAGCGCCGCCGCAAAAAGTGGTGGCGGTGCCGCACCGGCGAAAGCCGAGGCTCCGAAAGCGGACGCTCC
CAAGGCTGAGGCACCAAAGGCTGAACCTGCTGCCGCAGCCGCTCCCAAGGCAGAGCCGGCTCCGGTCGCCAATGGCCACG
CGGCTGGCGAGCGCACCTTCGCTTCGCCGCTCGCGCGCCGCATCGCCAAGGAGGCCGGCGTCGACGTGTCGGCCGTGACC
GGCACCGGCCCGCATGGCCGTGTGGTGAAGGCCGATGTCGATGCAGCGATTGCCGGCGGTGGTGCCAAGGCAGCCCTCGC
CGCCAAGGCTCCGGCCGGTGCGCCGGCGGCTCCCGCGCCGGCTGTGAAGCCGATGTCGGACGACCAGGTGCTGAAGCTGT
TCGAGCAAGGCTCCTACGAACTCGTCCCGCACGACAATATGCGCAAGACCATCGCGCGCCGGCTGGTCGAGGCCAAGTCC
ACCATCCCGCATTTCTACCTGACGCTCGACTGCGAACTCGATGCGTTGCTGGCCTTGCGCACGCAGATCAATGCCGCCGC
ACCGATGAAGAAGACCGAAAAGGGTGACGTGCCTGCCTACAAGCTGTCGGTCAACGACATGGTGATCAAGGCGATGGCGA
TGGCGCTGAAGGCGGTGCCGGATGCCAATGCCTCGTGGACCGAAACCGCCATGGTCAAGCACAAGCACGCCGATGTCGGC
GTTGCCGTGTCGATCCCCGGCGGCCTGATCACGCCGATCATCCGTAAGGCGGACGAAAAAACGCTGTCGACCATTTCCAA
CGAGATGAAGGACCTGGCCAGCCGCGCGCGCAGCCGCAAGCTGAAGCCGGAAGAGTATCAGGGCGGCACGACGGCGGTCT
CCAATCTCGGCATGTTCGGCATCAAGGACTTTGCCGCCGTCATCAATCCGCCGCATGCGACGATCCTGGCGGTCGGCGCC
GGTGAAGAGCGGGCTGTGGTCAAGAATGGCGAGCTCAAAATCGCCACCGTGATGTCGGTGACGCTGTCGACCGATCATCG
CGCCGTTGATGGCGCGCTCGGCGCCGAGCTGCTGGTCGCCTTCAAGCGGCTGATCGAGAACCCGATGGGCATGCTGGTCT
AG

Upstream 100 bases:

>100_bases
CTATGCGGCCAACCTCGAAAAGCTGGCTTTGCCGAATGTCGGCGAGGTCATCGAGGCGGTCAAAGCCGTCACATATCGCT
GAACCGGCCGGGAGGACAAA

Downstream 100 bases:

>100_bases
GAAAGGAAAAGGCTCGTGCGGAAATTCTTCACCAGCCTTTTCGCCTTTTTCATTTCAGGTTTCGCCGGCAGCCTCGTCGC
GCTCGGACTGGCCATCGCAA

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MPINITMPALSPTMEEGNLSKWLVKEGDKVSPGDVIAEIETDKATMEVEAVDEGTVAKLVVPAGTEGVKVNALIAVLAAE
GEDASAAAKSGGGAAPAKAEAPKADAPKAEAPKAEPAAAAAPKAEPAPVANGHAAGERTFASPLARRIAKEAGVDVSAVT
GTGPHGRVVKADVDAAIAGGGAKAALAAKAPAGAPAAPAPAVKPMSDDQVLKLFEQGSYELVPHDNMRKTIARRLVEAKS
TIPHFYLTLDCELDALLALRTQINAAAPMKKTEKGDVPAYKLSVNDMVIKAMAMALKAVPDANASWTETAMVKHKHADVG
VAVSIPGGLITPIIRKADEKTLSTISNEMKDLASRARSRKLKPEEYQGGTTAVSNLGMFGIKDFAAVINPPHATILAVGA
GEERAVVKNGELKIATVMSVTLSTDHRAVDGALGAELLVAFKRLIENPMGMLV

Sequences:

>Translated_453_residues
MPINITMPALSPTMEEGNLSKWLVKEGDKVSPGDVIAEIETDKATMEVEAVDEGTVAKLVVPAGTEGVKVNALIAVLAAE
GEDASAAAKSGGGAAPAKAEAPKADAPKAEAPKAEPAAAAAPKAEPAPVANGHAAGERTFASPLARRIAKEAGVDVSAVT
GTGPHGRVVKADVDAAIAGGGAKAALAAKAPAGAPAAPAPAVKPMSDDQVLKLFEQGSYELVPHDNMRKTIARRLVEAKS
TIPHFYLTLDCELDALLALRTQINAAAPMKKTEKGDVPAYKLSVNDMVIKAMAMALKAVPDANASWTETAMVKHKHADVG
VAVSIPGGLITPIIRKADEKTLSTISNEMKDLASRARSRKLKPEEYQGGTTAVSNLGMFGIKDFAAVINPPHATILAVGA
GEERAVVKNGELKIATVMSVTLSTDHRAVDGALGAELLVAFKRLIENPMGMLV
>Mature_452_residues
PINITMPALSPTMEEGNLSKWLVKEGDKVSPGDVIAEIETDKATMEVEAVDEGTVAKLVVPAGTEGVKVNALIAVLAAEG
EDASAAAKSGGGAAPAKAEAPKADAPKAEAPKAEPAAAAAPKAEPAPVANGHAAGERTFASPLARRIAKEAGVDVSAVTG
TGPHGRVVKADVDAAIAGGGAKAALAAKAPAGAPAAPAPAVKPMSDDQVLKLFEQGSYELVPHDNMRKTIARRLVEAKST
IPHFYLTLDCELDALLALRTQINAAAPMKKTEKGDVPAYKLSVNDMVIKAMAMALKAVPDANASWTETAMVKHKHADVGV
AVSIPGGLITPIIRKADEKTLSTISNEMKDLASRARSRKLKPEEYQGGTTAVSNLGMFGIKDFAAVINPPHATILAVGAG
EERAVVKNGELKIATVMSVTLSTDHRAVDGALGAELLVAFKRLIENPMGMLV

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=466, Percent_Identity=41.2017167381974, Blast_Score=332, Evalue=5e-91,
Organism=Homo sapiens, GI203098753, Length=473, Percent_Identity=38.9006342494715, Blast_Score=292, Evalue=5e-79,
Organism=Homo sapiens, GI203098816, Length=473, Percent_Identity=38.9006342494715, Blast_Score=291, Evalue=6e-79,
Organism=Homo sapiens, GI260898739, Length=160, Percent_Identity=44.375, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI110671329, Length=454, Percent_Identity=25.5506607929515, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI19923748, Length=231, Percent_Identity=30.7359307359307, Blast_Score=110, Evalue=4e-24,
Organism=Escherichia coli, GI1786946, Length=451, Percent_Identity=31.0421286031042, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1786305, Length=426, Percent_Identity=30.5164319248826, Blast_Score=147, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17560088, Length=461, Percent_Identity=42.7331887201735, Blast_Score=331, Evalue=5e-91,
Organism=Caenorhabditis elegans, GI17538894, Length=325, Percent_Identity=33.5384615384615, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI25146366, Length=456, Percent_Identity=30.4824561403509, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17537937, Length=461, Percent_Identity=28.1995661605206, Blast_Score=149, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6324258, Length=467, Percent_Identity=42.3982869379015, Blast_Score=317, Evalue=2e-87,
Organism=Saccharomyces cerevisiae, GI6320352, Length=232, Percent_Identity=32.3275862068966, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6321632, Length=179, Percent_Identity=32.4022346368715, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI20129315, Length=462, Percent_Identity=40.04329004329, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI24582497, Length=451, Percent_Identity=39.4678492239468, Blast_Score=265, Evalue=5e-71,
Organism=Drosophila melanogaster, GI18859875, Length=463, Percent_Identity=26.3498920086393, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24645909, Length=231, Percent_Identity=32.4675324675325, Blast_Score=108, Evalue=5e-24,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 46622; Mature: 46491

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPINITMPALSPTMEEGNLSKWLVKEGDKVSPGDVIAEIETDKATMEVEAVDEGTVAKLV
CCEEEECCCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCEEEEEEECCCCCEEEEE
VPAGTEGVKVNALIAVLAAEGEDASAAAKSGGGAAPAKAEAPKADAPKAEAPKAEPAAAA
EECCCCCEEEEEEEEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
APKAEPAPVANGHAAGERTFASPLARRIAKEAGVDVSAVTGTGPHGRVVKADVDAAIAGG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEECCCEEEECC
GAKAALAAKAPAGAPAAPAPAVKPMSDDQVLKLFEQGSYELVPHDNMRKTIARRLVEAKS
CCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHH
TIPHFYLTLDCELDALLALRTQINAAAPMKKTEKGDVPAYKLSVNDMVIKAMAMALKAVP
CCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCC
DANASWTETAMVKHKHADVGVAVSIPGGLITPIIRKADEKTLSTISNEMKDLASRARSRK
CCCCCCHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
LKPEEYQGGTTAVSNLGMFGIKDFAAVINPPHATILAVGAGEERAVVKNGELKIATVMSV
CCCHHCCCCHHHHHHCCCCCHHHHHHHCCCCCEEEEEEECCCCCEEEECCCEEEEEEEEE
TLSTDHRAVDGALGAELLVAFKRLIENPMGMLV
EECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PINITMPALSPTMEEGNLSKWLVKEGDKVSPGDVIAEIETDKATMEVEAVDEGTVAKLV
CEEEECCCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCEEEEEEECCCCCEEEEE
VPAGTEGVKVNALIAVLAAEGEDASAAAKSGGGAAPAKAEAPKADAPKAEAPKAEPAAAA
EECCCCCEEEEEEEEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
APKAEPAPVANGHAAGERTFASPLARRIAKEAGVDVSAVTGTGPHGRVVKADVDAAIAGG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEECCCEEEECC
GAKAALAAKAPAGAPAAPAPAVKPMSDDQVLKLFEQGSYELVPHDNMRKTIARRLVEAKS
CCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHH
TIPHFYLTLDCELDALLALRTQINAAAPMKKTEKGDVPAYKLSVNDMVIKAMAMALKAVP
CCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCC
DANASWTETAMVKHKHADVGVAVSIPGGLITPIIRKADEKTLSTISNEMKDLASRARSRK
CCCCCCHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
LKPEEYQGGTTAVSNLGMFGIKDFAAVINPPHATILAVGAGEERAVVKNGELKIATVMSV
CCCHHCCCCHHHHHHCCCCCHHHHHHHCCCCCEEEEEEECCCCCEEEECCCEEEEEEEEE
TLSTDHRAVDGALGAELLVAFKRLIENPMGMLV
EECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]