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The map label for this gene is pdhB [H]

Identifier: 13470620

GI number: 13470620

Start: 271824

End: 273209

Strand: Direct

Name: pdhB [H]

Synonym: mlr0384

Alternate gene names: 13470620

Gene position: 271824-273209 (Clockwise)

Preceding gene: 13470619

Following gene: 13470621

Centisome position: 3.86

GC content: 63.13

Gene sequence:

>1386_bases
ATGCCGATCGAAATTCTCATGCCCGCTCTCTCGCCAACCATGGAAGAGGGCAATCTTTCCAAGTGGTTGAAGAACGAGGG
CGACAAGGTCGTCGCCGGCGACGTCATCGCCGAGATCGAGACCGACAAGGCGACGATGGAGGTCGAAGCCGTCGATGAGG
GCACGCTCGCCAAGATCGTGGTTCCCGCCGGCACCGAGGGCGTCAAGGTCAACGCGGTGATCGCCGTGCTTGCGGTTGAA
GGCGAAGACACCGACAAGGCCGGCGAAGGCATCGGCGAAGAGCCCGCCAAGGCTGAAACGGCTTCACCGGCACCAGTTGC
TGCCAAGAGCGAGGCCGCCGCACCAGTCGCGGCAGCACCGAAGACCGAGATCGCCGCCGATCCTGACATCCCTGCCGGCA
CCGAAATGGTCTCGACCACGGTACGCGAAGCACTGCGCGATGCCATGGCCGAAGAGATGCGCCGCGACGGCGATGTCTTC
GTCATGGGCGAGGAGGTCGCCGAATACCAGGGCGCCTACAAGATCACCCAAGGCTTGCTGCAGGAATTCGGGCCGCGTCG
CGTCGTCGACACGCCGATCACCGAGCATGGTTTTGCCGGCGTCGGCGTCGGTGCGGCGATGGCTGGGCTGAAGCCGATCG
TCGAGTTCATGACCTTCAATTTCGCCATGCAGGCGATCGACCAGATCATCAATTCGGCCGCCAAGACGCTTTACATGTCC
GGCGGCCAGATGGGCGCGCCGATCGTATTCCGCGGACCGAACGGGGCCGCTGCCCGCGTCGCCGCCCAGCACTCGCAGTG
CTACGCCGCCTGGTACAGCCACATTCCGGGCCTGAAGGTGGTGATGCCGTATACGGCCGCTGACGCCAAGGGCCTGCTCA
AGGCGGCGATCCGCGACCCGAACCCGGTCATCTTTCTCGAGAACGAAATCCTCTACGGCCAGTCCTTCGACGTGCCGAAG
CTTGATGATTTCGTGCTGCCGATCGGCAAGGCCCGCATCCACAAGCAAGGCAAGGACGTCACCATCGTCTCCTTCGGCAT
CGGCATGACCTACGCGGTCAAGGCGGAAGCGGAATTGCGCGGCCTGGGCATCGACGCCGAGATCATCGATTTGCGCACCA
TCCGCCCGCTCGACCTCGACACCATCATCGCCTCGGTCAAGAAGACCAACCGGCTGGTCGTCGTTGAAGAAGGCTTCCCG
CAGAGCTCGGTCGGCGACCACATCGCCAACCAGGTGTCGCAGCGCGCTTTCGATTTCCTTGACGCGCCGGTTATCACCAT
TGCCGGCAAGGATGTGCCGATGCCCTATGCGGCCAACCTCGAAAAGCTGGCTTTGCCGAATGTCGGCGAGGTCATCGAGG
CGGTCAAAGCCGTCACATATCGCTGA

Upstream 100 bases:

>100_bases
CGACATCGTCGCCGACGCCGCCGAATTTGCCCAGAACGATGCGGAGCCGGATCCGTCCGAGCTCTGGACCGATATCGTAT
TGTAAGGGGAGGGCCAGGAC

Downstream 100 bases:

>100_bases
ACCGGCCGGGAGGACAAAATGCCAATCAACATCACCATGCCGGCGCTCTCGCCCACGATGGAAGAGGGCAATCTGTCGAA
ATGGCTGGTCAAGGAAGGCG

Product: pyruvate dehydrogenase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MPIEILMPALSPTMEEGNLSKWLKNEGDKVVAGDVIAEIETDKATMEVEAVDEGTLAKIVVPAGTEGVKVNAVIAVLAVE
GEDTDKAGEGIGEEPAKAETASPAPVAAKSEAAAPVAAAPKTEIAADPDIPAGTEMVSTTVREALRDAMAEEMRRDGDVF
VMGEEVAEYQGAYKITQGLLQEFGPRRVVDTPITEHGFAGVGVGAAMAGLKPIVEFMTFNFAMQAIDQIINSAAKTLYMS
GGQMGAPIVFRGPNGAAARVAAQHSQCYAAWYSHIPGLKVVMPYTAADAKGLLKAAIRDPNPVIFLENEILYGQSFDVPK
LDDFVLPIGKARIHKQGKDVTIVSFGIGMTYAVKAEAELRGLGIDAEIIDLRTIRPLDLDTIIASVKKTNRLVVVEEGFP
QSSVGDHIANQVSQRAFDFLDAPVITIAGKDVPMPYAANLEKLALPNVGEVIEAVKAVTYR

Sequences:

>Translated_461_residues
MPIEILMPALSPTMEEGNLSKWLKNEGDKVVAGDVIAEIETDKATMEVEAVDEGTLAKIVVPAGTEGVKVNAVIAVLAVE
GEDTDKAGEGIGEEPAKAETASPAPVAAKSEAAAPVAAAPKTEIAADPDIPAGTEMVSTTVREALRDAMAEEMRRDGDVF
VMGEEVAEYQGAYKITQGLLQEFGPRRVVDTPITEHGFAGVGVGAAMAGLKPIVEFMTFNFAMQAIDQIINSAAKTLYMS
GGQMGAPIVFRGPNGAAARVAAQHSQCYAAWYSHIPGLKVVMPYTAADAKGLLKAAIRDPNPVIFLENEILYGQSFDVPK
LDDFVLPIGKARIHKQGKDVTIVSFGIGMTYAVKAEAELRGLGIDAEIIDLRTIRPLDLDTIIASVKKTNRLVVVEEGFP
QSSVGDHIANQVSQRAFDFLDAPVITIAGKDVPMPYAANLEKLALPNVGEVIEAVKAVTYR
>Mature_460_residues
PIEILMPALSPTMEEGNLSKWLKNEGDKVVAGDVIAEIETDKATMEVEAVDEGTLAKIVVPAGTEGVKVNAVIAVLAVEG
EDTDKAGEGIGEEPAKAETASPAPVAAKSEAAAPVAAAPKTEIAADPDIPAGTEMVSTTVREALRDAMAEEMRRDGDVFV
MGEEVAEYQGAYKITQGLLQEFGPRRVVDTPITEHGFAGVGVGAAMAGLKPIVEFMTFNFAMQAIDQIINSAAKTLYMSG
GQMGAPIVFRGPNGAAARVAAQHSQCYAAWYSHIPGLKVVMPYTAADAKGLLKAAIRDPNPVIFLENEILYGQSFDVPKL
DDFVLPIGKARIHKQGKDVTIVSFGIGMTYAVKAEAELRGLGIDAEIIDLRTIRPLDLDTIIASVKKTNRLVVVEEGFPQ
SSVGDHIANQVSQRAFDFLDAPVITIAGKDVPMPYAANLEKLALPNVGEVIEAVKAVTYR

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=324, Percent_Identity=57.4074074074074, Blast_Score=394, Evalue=1e-110,
Organism=Homo sapiens, GI291084858, Length=324, Percent_Identity=53.7037037037037, Blast_Score=361, Evalue=1e-100,
Organism=Homo sapiens, GI4557353, Length=353, Percent_Identity=32.5779036827195, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI34101272, Length=353, Percent_Identity=32.5779036827195, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI203098753, Length=82, Percent_Identity=57.3170731707317, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI203098816, Length=82, Percent_Identity=57.3170731707317, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI260898739, Length=59, Percent_Identity=67.7966101694915, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI31711992, Length=82, Percent_Identity=45.1219512195122, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17538422, Length=323, Percent_Identity=62.2291021671827, Blast_Score=410, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17506935, Length=339, Percent_Identity=39.8230088495575, Blast_Score=197, Evalue=7e-51,
Organism=Caenorhabditis elegans, GI17560088, Length=120, Percent_Identity=39.1666666666667, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319698, Length=326, Percent_Identity=57.9754601226994, Blast_Score=392, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6324258, Length=133, Percent_Identity=45.1127819548872, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321632, Length=72, Percent_Identity=50, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21358145, Length=319, Percent_Identity=59.5611285266458, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24650940, Length=319, Percent_Identity=59.5611285266458, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI160714832, Length=318, Percent_Identity=34.5911949685535, Blast_Score=194, Evalue=2e-49,
Organism=Drosophila melanogaster, GI160714828, Length=318, Percent_Identity=34.5911949685535, Blast_Score=192, Evalue=3e-49,
Organism=Drosophila melanogaster, GI24650943, Length=89, Percent_Identity=62.9213483146067, Blast_Score=132, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24650945, Length=89, Percent_Identity=62.9213483146067, Blast_Score=132, Evalue=7e-31,
Organism=Drosophila melanogaster, GI20129315, Length=75, Percent_Identity=50.6666666666667, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24582497, Length=64, Percent_Identity=48.4375, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 48867; Mature: 48735

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIEILMPALSPTMEEGNLSKWLKNEGDKVVAGDVIAEIETDKATMEVEAVDEGTLAKIV
CCCEEEECCCCCCCCCCCHHHHHHCCCCEEEECCEEEEEECCCEEEEEEECCCCCEEEEE
VPAGTEGVKVNAVIAVLAVEGEDTDKAGEGIGEEPAKAETASPAPVAAKSEAAAPVAAAP
EECCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KTEIAADPDIPAGTEMVSTTVREALRDAMAEEMRRDGDVFVMGEEVAEYQGAYKITQGLL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHCCHHHHHHHHH
QEFGPRRVVDTPITEHGFAGVGVGAAMAGLKPIVEFMTFNFAMQAIDQIINSAAKTLYMS
HHCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEC
GGQMGAPIVFRGPNGAAARVAAQHSQCYAAWYSHIPGLKVVMPYTAADAKGLLKAAIRDP
CCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCC
NPVIFLENEILYGQSFDVPKLDDFVLPIGKARIHKQGKDVTIVSFGIGMTYAVKAEAELR
CCEEEEECEEEECCCCCCCCCCCHHCCCCHHHHHCCCCCEEEEEECCCEEEEEEECCHHE
GLGIDAEIIDLRTIRPLDLDTIIASVKKTNRLVVVEEGFPQSSVGDHIANQVSQRAFDFL
ECCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
DAPVITIAGKDVPMPYAANLEKLALPNVGEVIEAVKAVTYR
CCCEEEEECCCCCCCHHCCCHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
PIEILMPALSPTMEEGNLSKWLKNEGDKVVAGDVIAEIETDKATMEVEAVDEGTLAKIV
CCEEEECCCCCCCCCCCHHHHHHCCCCEEEECCEEEEEECCCEEEEEEECCCCCEEEEE
VPAGTEGVKVNAVIAVLAVEGEDTDKAGEGIGEEPAKAETASPAPVAAKSEAAAPVAAAP
EECCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
KTEIAADPDIPAGTEMVSTTVREALRDAMAEEMRRDGDVFVMGEEVAEYQGAYKITQGLL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHCCHHHHHHHHH
QEFGPRRVVDTPITEHGFAGVGVGAAMAGLKPIVEFMTFNFAMQAIDQIINSAAKTLYMS
HHCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEC
GGQMGAPIVFRGPNGAAARVAAQHSQCYAAWYSHIPGLKVVMPYTAADAKGLLKAAIRDP
CCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCC
NPVIFLENEILYGQSFDVPKLDDFVLPIGKARIHKQGKDVTIVSFGIGMTYAVKAEAELR
CCEEEEECEEEECCCCCCCCCCCHHCCCCHHHHHCCCCCEEEEEECCCEEEEEEECCHHE
GLGIDAEIIDLRTIRPLDLDTIIASVKKTNRLVVVEEGFPQSSVGDHIANQVSQRAFDFL
ECCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
DAPVITIAGKDVPMPYAANLEKLALPNVGEVIEAVKAVTYR
CCCEEEEECCCCCCCHHCCCHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]