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The map label for this gene is gltX

Identifier: 13358164

GI number: 13358164

Start: 746762

End: 748210

Strand: Reverse

Name: gltX

Synonym: UU599

Alternate gene names: 13358164

Gene position: 748210-746762 (Counterclockwise)

Preceding gene: 13358165

Following gene: 13358163

Centisome position: 99.53

GC content: 25.81

Gene sequence:

>1449_bases
ATGAAAATTAGAACACGTTATGCTCCATCACCAACCGGTTATTTACATATTGGAGGAGCGCGAACAGCTTTATTTAATTA
TTTACTAGCAAAAGCTTATGGTGGGGATTTTATTATTCGTATTGAAGATACTGATATTGAAAGAAATGTGGAAGGGGGAA
TTAATTCCCAACTAGATTTTTTAGCATGAATGGGTATTATTCCAGACGAATCAATTCGTAATCCTAAAGCTTTTGGCCCA
TATATCCAATCTGAAAAATTAAAACATTATGAAAAATTAGCATTAGATCTAGTTGATCAAAAAAAAGCTTATTTTTGTTT
TTGTAGTAAAGAACAATTAGATGCAGATCGCGAATTAGCTGAAAAATCACATCAAACACCTAAATACAAACGGCACTGTT
TAAATTTAGATAAAAAAACAATTGAAAGTAACTTATTACAAAATAAAGAATACACAATTCGTTTAAAAATTAATGAAAAT
ATGGAATATAGCTGGGATGATTTAATTCGGGGTAAAATTAGTATTCCAGGTAGTGCTTTAACTGATCCTGTCATTTTAAA
ATCAAATAAGATTGCAATGTATAATTTTGCAGTTGTAATTGACGATTATGAAATGCAAATTTCACATGTCATTCGTGGAG
AAGAGCATATTTCTAATACACCTTATCAATTAGCGATTGCTCAGGCCTTAAATTATGATATAACAAAAATTAAATATGGT
CATTTGTCAATTATTGTTGATGAGACAGGAAAAAAATTATCAAAGCGAAATTTATCTTTAAAACAGTTTGTGTCTGATTA
TGAAAAAGATGGTTATTGGCCACATGCTATTACAAACTTTGTTGCATTATTAGGTTGAAGCCCAAAAAATAACGATGAAA
TAATGAGTTTAGAAACGATGATTAAAAATTTTGATATCAATAATTTATCAAAATCCCCCGCCTTTTTTGATATCAATAAA
ATGAATTGGTTTTCAACACAATATTTTAATAATATTACCCAAGAAGAATTTATCAATTTTATAAAGAAACATTCATTAAC
TAAAGAATTAGTTTTGAATGATTATACTTTTATTAATAAATGTCTATTATTTAAATCTCATATTATTAATTTAAAACAAC
TAATTGATTTAGTAATTGAACAATTTAATTGTGATAAAAAAGTATTAGCATCAGATGTAGATTATATTAAAAAAAACCAA
TTAATAACTGTTGTTCGTGTTTTTTATGAACAATTAATAATTAATGATGAATTTAATGAAGAATTTATAAAAGAAATAAT
TAAAAAAGTTCAAATAATAACTAATAACAAAGGTGCTAATTTATACATGCCAATTCGTATTGCAACTACATTTAGTTCGC
ATGGTCCAGAATTAGCTAAAACAATTTGTTATTTAGGACGCGAAAAAGTATTAAAAAATTTAATAAATATTTTAAAAATT
TTGGATTAA

Upstream 100 bases:

>100_bases
TATAATGAATTATTATCCGATGGTTTTTATACAATTTTAAGTGGTGCAGGTAGTTCATTTATTCGCATAAAATTAAAAAA
TAAGGAAGATTTAATTATTA

Downstream 100 bases:

>100_bases
TAATTATTATGGTAAAAAATTTTTTTATAAAAGATCCTATTCATGGTTTAATTACGTTTGATGAACAATATGAATGAGCT
TATGAATTAATTAAAACAAA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MKIRTRYAPSPTGYLHIGGARTALFNYLLAKAYGGDFIIRIEDTDIERNVEGGINSQLDFLAWMGIIPDESIRNPKAFGP
YIQSEKLKHYEKLALDLVDQKKAYFCFCSKEQLDADRELAEKSHQTPKYKRHCLNLDKKTIESNLLQNKEYTIRLKINEN
MEYSWDDLIRGKISIPGSALTDPVILKSNKIAMYNFAVVIDDYEMQISHVIRGEEHISNTPYQLAIAQALNYDITKIKYG
HLSIIVDETGKKLSKRNLSLKQFVSDYEKDGYWPHAITNFVALLGWSPKNNDEIMSLETMIKNFDINNLSKSPAFFDINK
MNWFSTQYFNNITQEEFINFIKKHSLTKELVLNDYTFINKCLLFKSHIINLKQLIDLVIEQFNCDKKVLASDVDYIKKNQ
LITVVRVFYEQLIINDEFNEEFIKEIIKKVQIITNNKGANLYMPIRIATTFSSHGPELAKTICYLGREKVLKNLINILKI
LD

Sequences:

>Translated_482_residues
MKIRTRYAPSPTGYLHIGGARTALFNYLLAKAYGGDFIIRIEDTDIERNVEGGINSQLDFLA*MGIIPDESIRNPKAFGP
YIQSEKLKHYEKLALDLVDQKKAYFCFCSKEQLDADRELAEKSHQTPKYKRHCLNLDKKTIESNLLQNKEYTIRLKINEN
MEYSWDDLIRGKISIPGSALTDPVILKSNKIAMYNFAVVIDDYEMQISHVIRGEEHISNTPYQLAIAQALNYDITKIKYG
HLSIIVDETGKKLSKRNLSLKQFVSDYEKDGYWPHAITNFVALLG*SPKNNDEIMSLETMIKNFDINNLSKSPAFFDINK
MNWFSTQYFNNITQEEFINFIKKHSLTKELVLNDYTFINKCLLFKSHIINLKQLIDLVIEQFNCDKKVLASDVDYIKKNQ
LITVVRVFYEQLIINDEFNEEFIKEIIKKVQIITNNKGANLYMPIRIATTFSSHGPELAKTICYLGREKVLKNLINILKI
LD
>Mature_482_residues
MKIRTRYAPSPTGYLHIGGARTALFNYLLAKAYGGDFIIRIEDTDIERNVEGGINSQLDFLA*MGIIPDESIRNPKAFGP
YIQSEKLKHYEKLALDLVDQKKAYFCFCSKEQLDADRELAEKSHQTPKYKRHCLNLDKKTIESNLLQNKEYTIRLKINEN
MEYSWDDLIRGKISIPGSALTDPVILKSNKIAMYNFAVVIDDYEMQISHVIRGEEHISNTPYQLAIAQALNYDITKIKYG
HLSIIVDETGKKLSKRNLSLKQFVSDYEKDGYWPHAITNFVALLG*SPKNNDEIMSLETMIKNFDINNLSKSPAFFDINK
MNWFSTQYFNNITQEEFINFIKKHSLTKELVLNDYTFINKCLLFKSHIINLKQLIDLVIEQFNCDKKVLASDVDYIKKNQ
LITVVRVFYEQLIINDEFNEEFIKEIIKKVQIITNNKGANLYMPIRIATTFSSHGPELAKTICYLGREKVLKNLINILKI
LD

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=497, Percent_Identity=29.3762575452716, Blast_Score=233, Evalue=2e-61,
Organism=Escherichia coli, GI1788743, Length=497, Percent_Identity=31.7907444668008, Blast_Score=255, Evalue=5e-69,
Organism=Escherichia coli, GI226510925, Length=253, Percent_Identity=28.4584980237154, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71993394, Length=333, Percent_Identity=41.4414414414414, Blast_Score=225, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6324540, Length=342, Percent_Identity=36.8421052631579, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6324742, Length=336, Percent_Identity=24.1071428571429, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24665321, Length=510, Percent_Identity=30.7843137254902, Blast_Score=211, Evalue=1e-54,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 55501; Mature: 55501

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRTRYAPSPTGYLHIGGARTALFNYLLAKAYGGDFIIRIEDTDIERNVEGGINSQLDF
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCE
LAMGIIPDESIRNPKAFGPYIQSEKLKHYEKLALDLVDQKKAYFCFCSKEQLDADRELAE
EEEECCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHCCHHHHHHH
KSHQTPKYKRHCLNLDKKTIESNLLQNKEYTIRLKINENMEYSWDDLIRGKISIPGSALT
HHCCCHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHCCCEEECCCCCCC
DPVILKSNKIAMYNFAVVIDDYEMQISHVIRGEEHISNTPYQLAIAQALNYDITKIKYGH
CCEEEECCEEEEEEEEEEEECHHHHHHHHHCCHHHCCCCCHHHHHHHHHCCCCEEEEECE
LSIIVDETGKKLSKRNLSLKQFVSDYEKDGYWPHAITNFVALLGSPKNNDEIMSLETMIK
EEEEEECCCCHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHH
NFDINNLSKSPAFFDINKMNWFSTQYFNNITQEEFINFIKKHSLTKELVLNDYTFINKCL
HCCCCCCCCCCCEEEECCCCHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHCCHHHHHHHH
LFKSHIINLKQLIDLVIEQFNCDKKVLASDVDYIKKNQLITVVRVFYEQLIINDEFNEEF
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHH
IKEIIKKVQIITNNKGANLYMPIRIATTFSSHGPELAKTICYLGREKVLKNLINILKILD
HHHHHHHHEEEECCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIRTRYAPSPTGYLHIGGARTALFNYLLAKAYGGDFIIRIEDTDIERNVEGGINSQLDF
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCE
LAMGIIPDESIRNPKAFGPYIQSEKLKHYEKLALDLVDQKKAYFCFCSKEQLDADRELAE
EEEECCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHCCHHHHHHH
KSHQTPKYKRHCLNLDKKTIESNLLQNKEYTIRLKINENMEYSWDDLIRGKISIPGSALT
HHCCCHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHCCCEEECCCCCCC
DPVILKSNKIAMYNFAVVIDDYEMQISHVIRGEEHISNTPYQLAIAQALNYDITKIKYGH
CCEEEECCEEEEEEEEEEEECHHHHHHHHHCCHHHCCCCCHHHHHHHHHCCCCEEEEECE
LSIIVDETGKKLSKRNLSLKQFVSDYEKDGYWPHAITNFVALLGSPKNNDEIMSLETMIK
EEEEEECCCCHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHH
NFDINNLSKSPAFFDINKMNWFSTQYFNNITQEEFINFIKKHSLTKELVLNDYTFINKCL
HCCCCCCCCCCCEEEECCCCHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHCCHHHHHHHH
LFKSHIINLKQLIDLVIEQFNCDKKVLASDVDYIKKNQLITVVRVFYEQLIINDEFNEEF
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHH
IKEIIKKVQIITNNKGANLYMPIRIATTFSSHGPELAKTICYLGREKVLKNLINILKILD
HHHHHHHHEEEECCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA