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The map label for this gene is tsr

Identifier: 13358161

GI number: 13358161

Start: 744129

End: 745001

Strand: Reverse

Name: tsr

Synonym: UU596

Alternate gene names: 13358161

Gene position: 745001-744129 (Counterclockwise)

Preceding gene: 13358162

Following gene: 13358160

Centisome position: 99.11

GC content: 27.95

Gene sequence:

>873_bases
ATGTCACCATTAGTTAATGCCAAAAAAATGATTCAAAATGCTTATGAAAATCATTATGCAGTGGCTGCAATTAATATCAA
TAATTTAGAATGAATTAAAGCAGCATTATTAGCAGCTCAAGAAACAAACTCTCCTCTTTTATTAGCGACATCTGAAGGTG
CCGTAAAATATATGGGTGGTTATGATAATTGTTATGCAATGGTTGTTAATTTAATAAAACAAATGAATATTAAAACTCCT
GTTTGTTTGCATTTAGATCATGGTACATATGAAGGATGTATAAAAGCTATTAATGCTGGTTATTCCTCAATTATGTATGA
TGGATCAAAACTTTCGATCGAAGAAAACATTAAAAACACTAAAGAACTTTTGACAATAACTAAATTAAAAGGTGTATCAG
TAGAAGTTGAAGTTGGTTCAATTGGTGGGACAGAAGATGGGATTACTTCAGAAGGTGAATTAGCTAATATCGACGATTGT
TATCAAATGTGTTTATTAGATATTGATATGTTAGCGTGTGGTATTGGAAATATTCACGGAATATATCCTAAAAATTGAAA
AGGTTTAAATTTTAATTTATTAAAAGAAATTAACAATAAAGTGAATAAACCAATTGTTTTACACGGAGGTTCTGGTATTA
GTGATGAACAAATTTTAAAAGCAATTAATTTGGGTGTAGCAAAAATTAATATTAATACAGAATGTCAAATTGCATTTTCA
AATGCATTACAAGATCACTTAACAAAAGCAGGTGATTTAATTCTTTCTAAACAGTATGATCCACGAAAAATTTTGGCTTA
TGGTGTGGACGCAATTAAAAATACAATCATTGATAAATTCACTAAGTTTAATAGTTTAAATAAAATAAAATAA

Upstream 100 bases:

>100_bases
GTTGGCTACGAAGATGAAGATGAAGAATTTAAAAATAATAATACTTCTAATAAAGATGAAATAGAAGACGAAGAGGAATT
AGAAGAGGAAGAATAAAAAA

Downstream 100 bases:

>100_bases
GGAGAATAAAATGTCGTTATCAGCAGGTCTTGTTGGTTTGCCAAACGTTGGTAAATCCACATTATTTAATACAATTACAA
ATTCTAGAGTTGAGGCAGCT

Product: fructose-bisphosphate aldolase

Products: NA

Alternate protein names: FBP aldolase; FBPA; Fructose-1,6-bisphosphate aldolase [H]

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MSPLVNAKKMIQNAYENHYAVAAININNLEWIKAALLAAQETNSPLLLATSEGAVKYMGGYDNCYAMVVNLIKQMNIKTP
VCLHLDHGTYEGCIKAINAGYSSIMYDGSKLSIEENIKNTKELLTITKLKGVSVEVEVGSIGGTEDGITSEGELANIDDC
YQMCLLDIDMLACGIGNIHGIYPKNWKGLNFNLLKEINNKVNKPIVLHGGSGISDEQILKAINLGVAKININTECQIAFS
NALQDHLTKAGDLILSKQYDPRKILAYGVDAIKNTIIDKFTKFNSLNKIK

Sequences:

>Translated_290_residues
MSPLVNAKKMIQNAYENHYAVAAININNLE*IKAALLAAQETNSPLLLATSEGAVKYMGGYDNCYAMVVNLIKQMNIKTP
VCLHLDHGTYEGCIKAINAGYSSIMYDGSKLSIEENIKNTKELLTITKLKGVSVEVEVGSIGGTEDGITSEGELANIDDC
YQMCLLDIDMLACGIGNIHGIYPKN*KGLNFNLLKEINNKVNKPIVLHGGSGISDEQILKAINLGVAKININTECQIAFS
NALQDHLTKAGDLILSKQYDPRKILAYGVDAIKNTIIDKFTKFNSLNKIK
>Mature_289_residues
SPLVNAKKMIQNAYENHYAVAAININNLE*IKAALLAAQETNSPLLLATSEGAVKYMGGYDNCYAMVVNLIKQMNIKTPV
CLHLDHGTYEGCIKAINAGYSSIMYDGSKLSIEENIKNTKELLTITKLKGVSVEVEVGSIGGTEDGITSEGELANIDDCY
QMCLLDIDMLACGIGNIHGIYPKN*KGLNFNLLKEINNKVNKPIVLHGGSGISDEQILKAINLGVAKININTECQIAFSN
ALQDHLTKAGDLILSKQYDPRKILAYGVDAIKNTIIDKFTKFNSLNKIK

Specific function: Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3- phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis [H]

COG id: COG0191

COG function: function code G; Fructose/tagatose bisphosphate aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class II fructose-bisphosphate aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1789526, Length=290, Percent_Identity=34.4827586206897, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI87082041, Length=277, Percent_Identity=34.2960288808664, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI1788072, Length=241, Percent_Identity=35.2697095435685, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1789293, Length=346, Percent_Identity=26.5895953757225, Blast_Score=91, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6322790, Length=340, Percent_Identity=27.3529411764706, Blast_Score=99, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011289
- InterPro:   IPR000771 [H]

Pfam domain/function: PF01116 F_bP_aldolase [H]

EC number: =4.1.2.13 [H]

Molecular weight: Translated: 31305; Mature: 31173

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS00806 ALDOLASE_CLASS_II_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPLVNAKKMIQNAYENHYAVAAININNLEIKAALLAAQETNSPLLLATSEGAVKYMGGY
CCCCCHHHHHHHHHHHCCEEEEEEEECCEEEEEEEEEECCCCCCEEEEECCCCEEECCCC
DNCYAMVVNLIKQMNIKTPVCLHLDHGTYEGCIKAINAGYSSIMYDGSKLSIEENIKNTK
HHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCHHEEECCCEEEHHHHHHHHH
ELLTITKLKGVSVEVEVGSIGGTEDGITSEGELANIDDCYQMCLLDIDMLACGIGNIHGI
HHHHHEEECCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEE
YPKNKGLNFNLLKEINNKVNKPIVLHGGSGISDEQILKAINLGVAKININTECQIAFSNA
CCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCEEEEECCCEEEEEHHHH
LQDHLTKAGDLILSKQYDPRKILAYGVDAIKNTIIDKFTKFNSLNKIK
HHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SPLVNAKKMIQNAYENHYAVAAININNLEIKAALLAAQETNSPLLLATSEGAVKYMGGY
CCCCHHHHHHHHHHHCCEEEEEEEECCEEEEEEEEEECCCCCCEEEEECCCCEEECCCC
DNCYAMVVNLIKQMNIKTPVCLHLDHGTYEGCIKAINAGYSSIMYDGSKLSIEENIKNTK
HHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCHHEEECCCEEEHHHHHHHHH
ELLTITKLKGVSVEVEVGSIGGTEDGITSEGELANIDDCYQMCLLDIDMLACGIGNIHGI
HHHHHEEECCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEE
YPKNKGLNFNLLKEINNKVNKPIVLHGGSGISDEQILKAINLGVAKININTECQIAFSNA
CCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCEEEEECCCEEEEEHHHH
LQDHLTKAGDLILSKQYDPRKILAYGVDAIKNTIIDKFTKFNSLNKIK
HHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11048724 [H]