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The map label for this gene is uvrD

Identifier: 13358064

GI number: 13358064

Start: 617250

End: 619481

Strand: Reverse

Name: uvrD

Synonym: UU501

Alternate gene names: 13358064

Gene position: 619481-617250 (Counterclockwise)

Preceding gene: 13358065

Following gene: 13358063

Centisome position: 82.41

GC content: 26.3

Gene sequence:

>2232_bases
ATGGGATTAGGAATTGATTATTCACGCTTAAATCAAGAACAAAAAGAAGCAGTTACAGCAGACTTAGGACCGCAATTAGT
AATTGCAGGGGCAGGGACAGGAAAAACATCTGTTTTAACTTTACGTATTGCTTATTTAATTACTGAAAAAAATATTCATC
CATCACGGATTTTAGGTTTTACTTTTACTAATAAGGCTGCTGATGAAATGAAAGAACGTGTTGGAAAAACAATTGGCGTT
TCTATTCCTTATTTAAGTACTTTTCATTCAATGTGTGTAAAAATTTTACAACAAGATATTCATTATTTAAATTATCATAA
CAATATTAAAATTATTGATACAGACGATCAAGAAGTTTTGCTTAAAGAAATTTTTGATCAATTAAACATTGAAAAAAAAT
CGCAAGTTATTAAGAAAATAATTAAAACGATTAGTAAAGTTAAAAATAAATTTTTTGATCAAAACGATATGCTAAATGAA
AAAAATCACAAGTATTTAGAATTGGTTGATTTAAATGATGCACAAAGATTAGTTGATATTTATAAGATTTATTGTGATCG
ATGTTTTAAATTAAATTATGTTGATTTTGATGATTTGATCAATTTAACTCATAAGTTATTTATTGAGTTTCCAGAAGTTT
TAGAAAAATGACAAAATAAATTTGATTATATTCTTGTTGATGAATTTCAAGATACTAATAAAATTCAATATGATTTAATT
AGTTTATTAGCAACTAAACATCAAAATTTATTTGTGGTTGGCGACCCTGATCAAATGATTTATTCATTTAGAGGTGCTGA
ACAATGAATTATTAATAATTTTAGTCAAAATTTTAAGAATACAAAAGTAACAATTTTAAAAACAAATTATCGTTCAACTC
AGCCAATTTTAAATACAGCTAATAGATTAATCGATGCTAATAACAACAATTATAAAAAAAATTTAACTGCTTTTAATACT
AATGACAATAATTTACCAATTTATTTACGAGGGCAAAACCCAATTGATGAAGCTAATTGAATTGCTAGAAAAATTAGAGA
ATTGTTAGAAGAAGGAACGCCAGCTAACCAAATCGCTGTTTTGTTCCGAAGCAATCACTATTCTCGTACGATTGAACAAT
CAATGATGCGCGAGAGCATTCCTTATACAATTCTTGGCAGTAAAAAGTTTTATGAACGTGCTGAAATTAAGGACATGATT
GCTTATTTAAAAGTAGTTAATGATTTAGATGAACTATCTTTTTTAAGGATTATTAATACACCACGTCGTGCGATTGGTCC
AACTACTTTTGAACATGTTAAACATTATGCAATAAATAATAATTTATTTTTATTTGAAGCATTAGCTGAAGTTGAAAAAA
ACCATTTAATAAATAACACACAAAAAAAGAATATTTTAAATTTTGTTAATTTAATTAAAGAAATCCGTGATGAAATGGAA
GATTTAAAAATTCATGAGATTTTAGAATTAATTTACAAAAAAGTTAACTATGAAGCTTATTTATTAGAAAACGAAAAAGC
AGAAGATAAAATCGATAATGTTTATGAATTAAAACGCGCTATGAAAATGTATGTTGATCGTCATCCAGATGACACGATTA
ATGATTATTTAAATTCGATTGCTTTATATTTGAATAAGGATGGTAAAGATAGTAAAGAAAATGTTTTATTAATGACAGTT
CATAATTCTAAAGGTCTAGAATATGAAAACGTTTTCGTTGCTGGTATGAACGAAGGACTTCTTCCTTCTGATCGAGCAAT
TAATGATGATCCAATTAAAGGAGTTGAAGAAGAACGAAGAATTGCTTATGTAGCCTTAACACGAGCAAAAAAAAATCTTT
ATATTTCATCTGCTTGTTGTTATGATCCATTAGCAAGAAGACAAGTATTTGAAAGTCGTTTTATTAATGAAATTGGTTTT
AATAATTTAAAGATTATAAATAGTAGTTTTAAAAATAATAAACCAGAGGATATGCCATTAAAATCATTCTTAAAACAACA
AGAAGAACGTTCATGGTTCGATTCAAAACAAAAACAAAAAGAAGTTGAAGATAATTTTTATCAAACAATGAAAAACGATT
TTGAAATTGGTGAAAGAATTGTTCACACAAGTTTTGGTGATGGTGTTATTATTGGAATTGATGGTGATATCTTAACAATT
GCTTTCAGTAAATATCGTCAACCAAAACAAATTAATTTTAATCATAAAACAATCGTAAGAAGAATAGGTTAA

Upstream 100 bases:

>100_bases
GCCTAAACATAAAACAAAACGTGAAGTTGAGATTCAAAAAATCGATAATTTAATTCAAGAACTAGATAAAATATAAAATG
AAAAATAAGGAGAAGCTTTT

Downstream 100 bases:

>100_bases
AAAATGAAAATTTTAGCTATTGGAGATATTTTTTCAAAACAAGGAAGAAAAGCAGTGGCCAATGAATTGCCCAAGCTAAA
ACAAGAGTATCAAATTGATT

Product: DNA helicase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 743; Mature: 742

Protein sequence:

>743_residues
MGLGIDYSRLNQEQKEAVTADLGPQLVIAGAGTGKTSVLTLRIAYLITEKNIHPSRILGFTFTNKAADEMKERVGKTIGV
SIPYLSTFHSMCVKILQQDIHYLNYHNNIKIIDTDDQEVLLKEIFDQLNIEKKSQVIKKIIKTISKVKNKFFDQNDMLNE
KNHKYLELVDLNDAQRLVDIYKIYCDRCFKLNYVDFDDLINLTHKLFIEFPEVLEKWQNKFDYILVDEFQDTNKIQYDLI
SLLATKHQNLFVVGDPDQMIYSFRGAEQWIINNFSQNFKNTKVTILKTNYRSTQPILNTANRLIDANNNNYKKNLTAFNT
NDNNLPIYLRGQNPIDEANWIARKIRELLEEGTPANQIAVLFRSNHYSRTIEQSMMRESIPYTILGSKKFYERAEIKDMI
AYLKVVNDLDELSFLRIINTPRRAIGPTTFEHVKHYAINNNLFLFEALAEVEKNHLINNTQKKNILNFVNLIKEIRDEME
DLKIHEILELIYKKVNYEAYLLENEKAEDKIDNVYELKRAMKMYVDRHPDDTINDYLNSIALYLNKDGKDSKENVLLMTV
HNSKGLEYENVFVAGMNEGLLPSDRAINDDPIKGVEEERRIAYVALTRAKKNLYISSACCYDPLARRQVFESRFINEIGF
NNLKIINSSFKNNKPEDMPLKSFLKQQEERSWFDSKQKQKEVEDNFYQTMKNDFEIGERIVHTSFGDGVIIGIDGDILTI
AFSKYRQPKQINFNHKTIVRRIG

Sequences:

>Translated_743_residues
MGLGIDYSRLNQEQKEAVTADLGPQLVIAGAGTGKTSVLTLRIAYLITEKNIHPSRILGFTFTNKAADEMKERVGKTIGV
SIPYLSTFHSMCVKILQQDIHYLNYHNNIKIIDTDDQEVLLKEIFDQLNIEKKSQVIKKIIKTISKVKNKFFDQNDMLNE
KNHKYLELVDLNDAQRLVDIYKIYCDRCFKLNYVDFDDLINLTHKLFIEFPEVLEK*QNKFDYILVDEFQDTNKIQYDLI
SLLATKHQNLFVVGDPDQMIYSFRGAEQ*IINNFSQNFKNTKVTILKTNYRSTQPILNTANRLIDANNNNYKKNLTAFNT
NDNNLPIYLRGQNPIDEAN*IARKIRELLEEGTPANQIAVLFRSNHYSRTIEQSMMRESIPYTILGSKKFYERAEIKDMI
AYLKVVNDLDELSFLRIINTPRRAIGPTTFEHVKHYAINNNLFLFEALAEVEKNHLINNTQKKNILNFVNLIKEIRDEME
DLKIHEILELIYKKVNYEAYLLENEKAEDKIDNVYELKRAMKMYVDRHPDDTINDYLNSIALYLNKDGKDSKENVLLMTV
HNSKGLEYENVFVAGMNEGLLPSDRAINDDPIKGVEEERRIAYVALTRAKKNLYISSACCYDPLARRQVFESRFINEIGF
NNLKIINSSFKNNKPEDMPLKSFLKQQEERSWFDSKQKQKEVEDNFYQTMKNDFEIGERIVHTSFGDGVIIGIDGDILTI
AFSKYRQPKQINFNHKTIVRRIG
>Mature_742_residues
GLGIDYSRLNQEQKEAVTADLGPQLVIAGAGTGKTSVLTLRIAYLITEKNIHPSRILGFTFTNKAADEMKERVGKTIGVS
IPYLSTFHSMCVKILQQDIHYLNYHNNIKIIDTDDQEVLLKEIFDQLNIEKKSQVIKKIIKTISKVKNKFFDQNDMLNEK
NHKYLELVDLNDAQRLVDIYKIYCDRCFKLNYVDFDDLINLTHKLFIEFPEVLEK*QNKFDYILVDEFQDTNKIQYDLIS
LLATKHQNLFVVGDPDQMIYSFRGAEQ*IINNFSQNFKNTKVTILKTNYRSTQPILNTANRLIDANNNNYKKNLTAFNTN
DNNLPIYLRGQNPIDEAN*IARKIRELLEEGTPANQIAVLFRSNHYSRTIEQSMMRESIPYTILGSKKFYERAEIKDMIA
YLKVVNDLDELSFLRIINTPRRAIGPTTFEHVKHYAINNNLFLFEALAEVEKNHLINNTQKKNILNFVNLIKEIRDEMED
LKIHEILELIYKKVNYEAYLLENEKAEDKIDNVYELKRAMKMYVDRHPDDTINDYLNSIALYLNKDGKDSKENVLLMTVH
NSKGLEYENVFVAGMNEGLLPSDRAINDDPIKGVEEERRIAYVALTRAKKNLYISSACCYDPLARRQVFESRFINEIGFN
NLKIINSSFKNNKPEDMPLKSFLKQQEERSWFDSKQKQKEVEDNFYQTMKNDFEIGERIVHTSFGDGVIIGIDGDILTIA
FSKYRQPKQINFNHKTIVRRIG

Specific function: Essential helicase. May act as a helicase in plasmid pT181 replication [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=730, Percent_Identity=32.1917808219178, Blast_Score=366, Evalue=1e-102,
Organism=Escherichia coli, GI48994965, Length=659, Percent_Identity=36.7223065250379, Blast_Score=327, Evalue=2e-90,
Organism=Escherichia coli, GI1787196, Length=406, Percent_Identity=27.0935960591133, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6322369, Length=762, Percent_Identity=29.5275590551181, Blast_Score=234, Evalue=5e-62,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 86130; Mature: 85998

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLGIDYSRLNQEQKEAVTADLGPQLVIAGAGTGKTSVLTLRIAYLITEKNIHPSRILGF
CCCCCCHHHHCHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEEEEEEECCCCCHHHEEEE
TFTNKAADEMKERVGKTIGVSIPYLSTFHSMCVKILQQDIHYLNYHNNIKIIDTDDQEVL
EECCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCEEEEECCCHHHH
LKEIFDQLNIEKKSQVIKKIIKTISKVKNKFFDQNDMLNEKNHKYLELVDLNDAQRLVDI
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHHHHH
YKIYCDRCFKLNYVDFDDLINLTHKLFIEFPEVLEKQNKFDYILVDEFQDTNKIQYDLIS
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHH
LLATKHQNLFVVGDPDQMIYSFRGAEQIINNFSQNFKNTKVTILKTNYRSTQPILNTANR
HHHHCCCCEEEEECHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHH
LIDANNNNYKKNLTAFNTNDNNLPIYLRGQNPIDEANIARKIRELLEEGTPANQIAVLFR
HEECCCCCCCCCEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHEEEEEE
SNHYSRTIEQSMMRESIPYTILGSKKFYERAEIKDMIAYLKVVNDLDELSFLRIINTPRR
CCCHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
AIGPTTFEHVKHYAINNNLFLFEALAEVEKNHLINNTQKKNILNFVNLIKEIRDEMEDLK
HCCCCHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IHEILELIYKKVNYEAYLLENEKAEDKIDNVYELKRAMKMYVDRHPDDTINDYLNSIALY
HHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEE
LNKDGKDSKENVLLMTVHNSKGLEYENVFVAGMNEGLLPSDRAINDDPIKGVEEERRIAY
ECCCCCCCCCCEEEEEEECCCCCEECCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHEEE
VALTRAKKNLYISSACCYDPLARRQVFESRFINEIGFNNLKIINSSFKNNKPEDMPLKSF
EEEEHHHCCEEEECHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECHHCCCCCCCCCHHHH
LKQQEERSWFDSKQKQKEVEDNFYQTMKNDFEIGERIVHTSFGDGVIIGIDGDILTIAFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCEEEEEHH
KYRQPKQINFNHKTIVRRIG
HCCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure 
GLGIDYSRLNQEQKEAVTADLGPQLVIAGAGTGKTSVLTLRIAYLITEKNIHPSRILGF
CCCCCHHHHCHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEEEEEEECCCCCHHHEEEE
TFTNKAADEMKERVGKTIGVSIPYLSTFHSMCVKILQQDIHYLNYHNNIKIIDTDDQEVL
EECCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCEEEEECCCHHHH
LKEIFDQLNIEKKSQVIKKIIKTISKVKNKFFDQNDMLNEKNHKYLELVDLNDAQRLVDI
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHHHHH
YKIYCDRCFKLNYVDFDDLINLTHKLFIEFPEVLEKQNKFDYILVDEFQDTNKIQYDLIS
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHH
LLATKHQNLFVVGDPDQMIYSFRGAEQIINNFSQNFKNTKVTILKTNYRSTQPILNTANR
HHHHCCCCEEEEECHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHH
LIDANNNNYKKNLTAFNTNDNNLPIYLRGQNPIDEANIARKIRELLEEGTPANQIAVLFR
HEECCCCCCCCCEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHEEEEEE
SNHYSRTIEQSMMRESIPYTILGSKKFYERAEIKDMIAYLKVVNDLDELSFLRIINTPRR
CCCHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
AIGPTTFEHVKHYAINNNLFLFEALAEVEKNHLINNTQKKNILNFVNLIKEIRDEMEDLK
HCCCCHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
IHEILELIYKKVNYEAYLLENEKAEDKIDNVYELKRAMKMYVDRHPDDTINDYLNSIALY
HHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEE
LNKDGKDSKENVLLMTVHNSKGLEYENVFVAGMNEGLLPSDRAINDDPIKGVEEERRIAY
ECCCCCCCCCCEEEEEEECCCCCEECCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHEEE
VALTRAKKNLYISSACCYDPLARRQVFESRFINEIGFNNLKIINSSFKNNKPEDMPLKSF
EEEEHHHCCEEEECHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECHHCCCCCCCCCHHHH
LKQQEERSWFDSKQKQKEVEDNFYQTMKNDFEIGERIVHTSFGDGVIIGIDGDILTIAFS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCEEEEEHH
KYRQPKQINFNHKTIVRRIG
HCCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8232203 [H]