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The map label for this gene is dnaG

Identifier: 13358057

GI number: 13358057

Start: 591955

End: 593880

Strand: Direct

Name: dnaG

Synonym: UU494

Alternate gene names: 13358057

Gene position: 591955-593880 (Clockwise)

Preceding gene: 13358056

Following gene: 13358060

Centisome position: 78.75

GC content: 22.07

Gene sequence:

>1926_bases
ATGTCAAAAATAACTAACAATGTTTTTTTAACAATTCGTAATGCTATAAATATTTCCGATGTTATTCAAAATTACATTAA
AGTAATTAAAAAGGGTAATAATTATTGAGCTATTTGTCCATTTCACAATGATACAAATGAATCATTGTCAATCAATGATA
AAAAACAAATCTTTAAATGTTTTGCTTGTAATCATGCTGGTGATGTGATTAAATTTGTTGCTGAATATAAAAAGATCTCC
TATGCTTTAGCAGCACAAGAGATTGTTCAATTAATGAATTTAGATTTAAACTTATTGAATCATTTACAAAAAATTTCTCC
ACAAGAAATTAAAAAAAATAAGGTTTTTGATTTAAACAAACAAATTCAAAAATGATTTATTAATTTTTTAAATAATAAAA
ATAACATTCATGTTATTGATTATTTAACTAAACGAAATTTAAATAAAAATGATTTAGCATATTTTAAAATCGGTTATGCT
CCAAATAATGATGAATTATTAAATTTAATAAAATCTAATTATAATAATCTGATACAAGAAAATGACGAGTTTGAATTAAA
TAAATTAATAGAAAATTCCTTTTTAGTAATTGATAAGAATGGTAATTACCATGATTTTTTTAATGATCGTATTATTTTTA
GTATTTATGATCATCTCAATAACGTTGTTGGTTTTAGCGGACGTATAATTACAAATGAGAAAACACCAAAATATTTAAAT
ACTAAAACCACTGCTGTATTTAAAAAAGATGAAGTTCTTTATAATTTTCATAATGTTATCACCATTGAAAATAATCATCA
ATTATTTATAGTTGAAGGTTTTATGGATGTTATTACAATTCATAAATCTAACAAACCAAACGTTGTAGCAACAATGGGTG
TTGAATTAACTAATAAACATTTAGAATTAATTAAAAGCTATGGAATTAAAGATTTAATTTTATGTTTTGATAACGATCAA
GCAGGTTTTTTAGCTACTAAAAAACAAATTATGAAATTAATTAATTCCCCATTCAACATTTTTATTGTTAATCATAAAGA
TAATGATTGCAAAGACATGGATGAATATGCAAATAAGCACGGTTATAGTGCAACAAGTAAGCTATTAGACCAACAACTTC
ATATTAGTGAATTTTTATTATTAGAAATTAGTAATACAAACAAATCTACCCCCTCACTAAAACACAGTTATAAACAAGAG
TGTTTTACAATCATTAAAAAATATGGTGATATTTTTTATGTAGATAAATATGTTGATTTCATGAAACAAACATTTTCAAT
TGAAGAAAATTTATTAAAAAAAGTTATTGAAGAATTATTAATTAACAAACAATGTAATTCTATTCAAAACAAAAAGAACA
TTCAATCATATCAAAAACAAACACAACTAACTAATAAACTTACTACTTCAAACCCAAAAGTTATCCTAACAAAAATTGAT
AAACATTTTAACCACTTAGTAGTAATTATATTAATGAATCGGGTTTTCTTAAACCGTCTTGATACGCAAGTTCGTAACCG
GTTAACTAAAGAGCAAAGTTTAGTGATTAATATTATAGAAGACTTTTATTTGTACCATTTTAAGATTAATAATATTTTAA
AAAACACTGATTCTTTTATTGAATTTATTAATAAAAACTACAGTAAATATACTAATGTTATTAACCAACTTACAAAAATG
GTGCAGATTATTAAATATAACCCTAAGTTTGATAATATAGTGGCATTTGAACTAGCTAAAAATAATTTTATATATGAATG
ATTAAACAACCAAATTAAACGTTTAAAACTCATCAATCTAAATTTAAATATGAATAATAACCAAGAACAAATCATAAATA
ATGAAAAATTAATAAAGCAATATAATGATGAAAAAATTTTATTAGACAATTTAATCAATGCTTCTTTATTTCATAAAAAT
CAATAA

Upstream 100 bases:

>100_bases
TTACTATTCGTGAACGAAACACAATGGCTCAAAAAAGAATTAAATTATCAGAGTTATCTTTATATTTAAGCCAAAAAGCT
CATGAAGATTTTCAAAAGCA

Downstream 100 bases:

>100_bases
TGAATACAAAAAAGCTTTTTAGTAATGTTTGTTTACTAGAAAGCTTTTTTTATTATTAATCAATTAGTTTAAATGTAATT
AAAATTGGTTTTCGTTATTT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 641; Mature: 640

Protein sequence:

>641_residues
MSKITNNVFLTIRNAINISDVIQNYIKVIKKGNNYWAICPFHNDTNESLSINDKKQIFKCFACNHAGDVIKFVAEYKKIS
YALAAQEIVQLMNLDLNLLNHLQKISPQEIKKNKVFDLNKQIQKWFINFLNNKNNIHVIDYLTKRNLNKNDLAYFKIGYA
PNNDELLNLIKSNYNNLIQENDEFELNKLIENSFLVIDKNGNYHDFFNDRIIFSIYDHLNNVVGFSGRIITNEKTPKYLN
TKTTAVFKKDEVLYNFHNVITIENNHQLFIVEGFMDVITIHKSNKPNVVATMGVELTNKHLELIKSYGIKDLILCFDNDQ
AGFLATKKQIMKLINSPFNIFIVNHKDNDCKDMDEYANKHGYSATSKLLDQQLHISEFLLLEISNTNKSTPSLKHSYKQE
CFTIIKKYGDIFYVDKYVDFMKQTFSIEENLLKKVIEELLINKQCNSIQNKKNIQSYQKQTQLTNKLTTSNPKVILTKID
KHFNHLVVIILMNRVFLNRLDTQVRNRLTKEQSLVINIIEDFYLYHFKINNILKNTDSFIEFINKNYSKYTNVINQLTKM
VQIIKYNPKFDNIVAFELAKNNFIYEWLNNQIKRLKLINLNLNMNNNQEQIINNEKLIKQYNDEKILLDNLINASLFHKN
Q

Sequences:

>Translated_641_residues
MSKITNNVFLTIRNAINISDVIQNYIKVIKKGNNY*AICPFHNDTNESLSINDKKQIFKCFACNHAGDVIKFVAEYKKIS
YALAAQEIVQLMNLDLNLLNHLQKISPQEIKKNKVFDLNKQIQK*FINFLNNKNNIHVIDYLTKRNLNKNDLAYFKIGYA
PNNDELLNLIKSNYNNLIQENDEFELNKLIENSFLVIDKNGNYHDFFNDRIIFSIYDHLNNVVGFSGRIITNEKTPKYLN
TKTTAVFKKDEVLYNFHNVITIENNHQLFIVEGFMDVITIHKSNKPNVVATMGVELTNKHLELIKSYGIKDLILCFDNDQ
AGFLATKKQIMKLINSPFNIFIVNHKDNDCKDMDEYANKHGYSATSKLLDQQLHISEFLLLEISNTNKSTPSLKHSYKQE
CFTIIKKYGDIFYVDKYVDFMKQTFSIEENLLKKVIEELLINKQCNSIQNKKNIQSYQKQTQLTNKLTTSNPKVILTKID
KHFNHLVVIILMNRVFLNRLDTQVRNRLTKEQSLVINIIEDFYLYHFKINNILKNTDSFIEFINKNYSKYTNVINQLTKM
VQIIKYNPKFDNIVAFELAKNNFIYE*LNNQIKRLKLINLNLNMNNNQEQIINNEKLIKQYNDEKILLDNLINASLFHKN
Q
>Mature_640_residues
SKITNNVFLTIRNAINISDVIQNYIKVIKKGNNY*AICPFHNDTNESLSINDKKQIFKCFACNHAGDVIKFVAEYKKISY
ALAAQEIVQLMNLDLNLLNHLQKISPQEIKKNKVFDLNKQIQK*FINFLNNKNNIHVIDYLTKRNLNKNDLAYFKIGYAP
NNDELLNLIKSNYNNLIQENDEFELNKLIENSFLVIDKNGNYHDFFNDRIIFSIYDHLNNVVGFSGRIITNEKTPKYLNT
KTTAVFKKDEVLYNFHNVITIENNHQLFIVEGFMDVITIHKSNKPNVVATMGVELTNKHLELIKSYGIKDLILCFDNDQA
GFLATKKQIMKLINSPFNIFIVNHKDNDCKDMDEYANKHGYSATSKLLDQQLHISEFLLLEISNTNKSTPSLKHSYKQEC
FTIIKKYGDIFYVDKYVDFMKQTFSIEENLLKKVIEELLINKQCNSIQNKKNIQSYQKQTQLTNKLTTSNPKVILTKIDK
HFNHLVVIILMNRVFLNRLDTQVRNRLTKEQSLVINIIEDFYLYHFKINNILKNTDSFIEFINKNYSKYTNVINQLTKMV
QIIKYNPKFDNIVAFELAKNNFIYE*LNNQIKRLKLINLNLNMNNNQEQIINNEKLIKQYNDEKILLDNLINASLFHKNQ

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=472, Percent_Identity=26.0593220338983, Blast_Score=173, Evalue=3e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 74779; Mature: 74648

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKITNNVFLTIRNAINISDVIQNYIKVIKKGNNYAICPFHNDTNESLSINDKKQIFKCF
CCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHH
ACNHAGDVIKFVAEYKKISYALAAQEIVQLMNLDLNLLNHLQKISPQEIKKNKVFDLNKQ
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHCCCCCCHHHH
IQKFINFLNNKNNIHVIDYLTKRNLNKNDLAYFKIGYAPNNDELLNLIKSNYNNLIQEND
HHHHHHHHCCCCCEEEEEEHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCC
EFELNKLIENSFLVIDKNGNYHDFFNDRIIFSIYDHLNNVVGFSGRIITNEKTPKYLNTK
CHHHHHHHCCCEEEEECCCCCCCCCCCEEEEHHHHHHHHHCCCCCEEEECCCCCCEECCC
TTAVFKKDEVLYNFHNVITIENNHQLFIVEGFMDVITIHKSNKPNVVATMGVELTNKHLE
EEEEEECCHHEEEEEEEEEEECCCEEEEEECCEEEEEEECCCCCCEEEEECCHHHHHHHH
LIKSYGIKDLILCFDNDQAGFLATKKQIMKLINSPFNIFIVNHKDNDCKDMDEYANKHGY
HHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCC
SATSKLLDQQLHISEFLLLEISNTNKSTPSLKHSYKQECFTIIKKYGDIFYVDKYVDFMK
HHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHH
QTFSIEENLLKKVIEELLINKQCNSIQNKKNIQSYQKQTQLTNKLTTSNPKVILTKIDKH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHH
FNHLVVIILMNRVFLNRLDTQVRNRLTKEQSLVINIIEDFYLYHFKINNILKNTDSFIEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHCHHHHHHH
INKNYSKYTNVINQLTKMVQIIKYNPKFDNIVAFELAKNNFIYELNNQIKRLKLINLNLN
HHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEECCCEEEEEEEEEEEC
MNNNQEQIINNEKLIKQYNDEKILLDNLINASLFHKNQ
CCCCHHHHHCHHHHHHHCCCCEEEHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKITNNVFLTIRNAINISDVIQNYIKVIKKGNNYAICPFHNDTNESLSINDKKQIFKCF
CCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHH
ACNHAGDVIKFVAEYKKISYALAAQEIVQLMNLDLNLLNHLQKISPQEIKKNKVFDLNKQ
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHCCCCCCHHHH
IQKFINFLNNKNNIHVIDYLTKRNLNKNDLAYFKIGYAPNNDELLNLIKSNYNNLIQEND
HHHHHHHHCCCCCEEEEEEHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCC
EFELNKLIENSFLVIDKNGNYHDFFNDRIIFSIYDHLNNVVGFSGRIITNEKTPKYLNTK
CHHHHHHHCCCEEEEECCCCCCCCCCCEEEEHHHHHHHHHCCCCCEEEECCCCCCEECCC
TTAVFKKDEVLYNFHNVITIENNHQLFIVEGFMDVITIHKSNKPNVVATMGVELTNKHLE
EEEEEECCHHEEEEEEEEEEECCCEEEEEECCEEEEEEECCCCCCEEEEECCHHHHHHHH
LIKSYGIKDLILCFDNDQAGFLATKKQIMKLINSPFNIFIVNHKDNDCKDMDEYANKHGY
HHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCC
SATSKLLDQQLHISEFLLLEISNTNKSTPSLKHSYKQECFTIIKKYGDIFYVDKYVDFMK
HHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHH
QTFSIEENLLKKVIEELLINKQCNSIQNKKNIQSYQKQTQLTNKLTTSNPKVILTKIDKH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHH
FNHLVVIILMNRVFLNRLDTQVRNRLTKEQSLVINIIEDFYLYHFKINNILKNTDSFIEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHHCHHHHHHH
INKNYSKYTNVINQLTKMVQIIKYNPKFDNIVAFELAKNNFIYELNNQIKRLKLINLNLN
HHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEECCCEEEEEEEEEEEC
MNNNQEQIINNEKLIKQYNDEKILLDNLINASLFHKNQ
CCCCHHHHHCHHHHHHHCCCCEEEHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11048724 [H]