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The map label for this gene is clpB

Identifier: 13357952

GI number: 13357952

Start: 451524

End: 453638

Strand: Direct

Name: clpB

Synonym: UU391

Alternate gene names: 13357952

Gene position: 451524-453638 (Clockwise)

Preceding gene: 13357951

Following gene: 13357953

Centisome position: 60.07

GC content: 27.09

Gene sequence:

>2115_bases
ATGGAATTTAAACAAAAAAACTCAAACAATCCATTAGAGGAATTTGGACGTAATTTAAATAAGGAAATTTTAGATAATAA
AATTGACCCCATTATCGGTCGAGATGAAGAAATTAGAAGAACCATTGAAATTCTAAGTCGTAAAACTAAGAATAATCCTG
TCTTAATTGGTGAACCTGGTGTTGGTAAAACAGCAATTGTTGAGGGGCTTGCATACCGAATTGTTCATAAAGATGTTCCT
TCAAACTTATTAGATAAAACAATTATTGAATTATCATTATCATCTTTAATTGCAGGCGCTAGTTATCAAGGCCAGTTTGA
GGAACGAATTAATAGTATTTTAAAAGAAGTCAAAAAAGCAGATGGTAATATCATTTTGTTTATTGATGAAATACATCAAA
TTGTTGGTATGGGTAGAAATCAAGGTTCAAATATGGATGTTGCTAATATTTTAAAACCAATGATGGCAAGAGGAGAAATC
AAATTAATTGGCGCCACAACGCTTGATGAGTATCGTTTATATATTGAAAAAGATCAGGCATTAGAACGTCGTTTTACAAA
AGTTTTAGTTAACAAACCAACAATTCAAGAAACTTTAACAATAATGCGAGGTTTAAAGCCACGGTGAGAAGCCTTTCATG
GGATTAAAATCCATGATAATGCATTAATTGCTGCTGTTAATTTATCTGAACGTTACATAAATGATCGTAATTTGCCAGAT
AAAGCTATTGATTTAATTGATGAAGCAGCTGCAAAGATTAAAACGCAAATTAATTCACAACCAATATATTTAGATGAAAT
TAAACGTGATTTACAACATTTACAAACTGAAAAAGCCGCTTTAGAACCAGAAAAAGATGAAAAATCAATTAAACGTTTAA
ATGAAATTTTAGAAAAAATTAAATTAAAACAAGATGAATTTAACAATTTAAATGATTTGTATTTAAAAGAAAAAAAGCAA
ATTGACGATTTAAAAAATTTACGGCAAAAAATTGAAAGAATTCAACATGATATTGAATTTTATCAATCTGAGGGTAAGTA
TGAAAAAGCATCAAGATTACTTTATTCAGATTTACCATTATTTGAAAAACAACGAGAAGAATTAGAAAATAAAATTAATG
AGAATAATAATCAAAAAATGATTATTGATTCATTAACAGAGAATGAAATTGCTGATGTTATTGCGCGTGCTACAGGAATT
CCATTAAGTCATTTATTAGCTGATGAAAAAACAAAATTATTATCGCTAAACAAACGAATTGCTAGTCATGTTATTGGTCA
AGATGAAGCAGTTAAATTAATAAGTGATGCGGTAATCCGTGGTCGTGCAGGTATTAATAATCCTAACCAACCTATTGGTT
CTTTCTTATTTTTAGGACCTACGGGCGTTGGTAAAACAGAATTAGCAAAAACTTTAGCAAAAGAATTGTTCAATTCAGAA
AAAGCCTTAATTAGATTTGATATGAGTGAATATATGGAAAAACATAGTGTATCAAAATTAGTTGGTGCTCCACCTGGTTA
TATTGGTTATGAAAATGCTGGAGTTTTAACTGAATCTGTTAAACGCAAACCATATAGTATTTTGTTATTTGATGAAATTG
AAAAAGCACACCCTGATATTTTAAATATTTTATTACAAATATTGGATGAAGGTAGCATTAAGGATGCTAAAAATAATGAA
ATTAATTTTAAAAATACAATTATTATTATGACATCTAATATTGGAGCAGAAGCTTTATTAGAAAATAATAAAACAAAGGC
ATTACTTGATTTACAAAAAACTTTTAAACCAGAAATTTTAAACCGAATTTCTGAAATTATTTTCTTTAATAAGTTATCAA
AAGAAATCATTTTTAAGATTAGTGAAAATTTACTTAAAGAATTAGAAAATTTATTAGCTAAGCAAGATTATTTAATTAAA
TTTGATGATAATATTGCTCAAATCATGGTTGAAGATGCTTATAGTTCAAATTATGGTGCAAGACCTTTAAAACGATGAAT
TACAAAACATTTAGAAAATGAAATTGCTAAATTAATAATTGAAAATCGTATTACAAAAAACATAAATTATAAAATAAATT
ATGATCGTGAACTAGATCAAGTTTTAATTAAATAA

Upstream 100 bases:

>100_bases
CAGGTTTGTTTAAACCTGTTTTTTTATGACAATACATTTATTTTTTAGCACTCATTATTTTAAAGTGCTAAAATTATAAT
AGTAAAGTAGGTGATTATTT

Downstream 100 bases:

>100_bases
TAATAAAAATCACCAACAATAAAATGGTGGTTTTTTATTCCAAATATTTAATTAAAACCTTTTTTTAGTAATATAAAGTA
AAAAAGATTATTTTTATTAA

Product: ATP-dependent serine proteinase - heat shock protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 704; Mature: 704

Protein sequence:

>704_residues
MEFKQKNSNNPLEEFGRNLNKEILDNKIDPIIGRDEEIRRTIEILSRKTKNNPVLIGEPGVGKTAIVEGLAYRIVHKDVP
SNLLDKTIIELSLSSLIAGASYQGQFEERINSILKEVKKADGNIILFIDEIHQIVGMGRNQGSNMDVANILKPMMARGEI
KLIGATTLDEYRLYIEKDQALERRFTKVLVNKPTIQETLTIMRGLKPRWEAFHGIKIHDNALIAAVNLSERYINDRNLPD
KAIDLIDEAAAKIKTQINSQPIYLDEIKRDLQHLQTEKAALEPEKDEKSIKRLNEILEKIKLKQDEFNNLNDLYLKEKKQ
IDDLKNLRQKIERIQHDIEFYQSEGKYEKASRLLYSDLPLFEKQREELENKINENNNQKMIIDSLTENEIADVIARATGI
PLSHLLADEKTKLLSLNKRIASHVIGQDEAVKLISDAVIRGRAGINNPNQPIGSFLFLGPTGVGKTELAKTLAKELFNSE
KALIRFDMSEYMEKHSVSKLVGAPPGYIGYENAGVLTESVKRKPYSILLFDEIEKAHPDILNILLQILDEGSIKDAKNNE
INFKNTIIIMTSNIGAEALLENNKTKALLDLQKTFKPEILNRISEIIFFNKLSKEIIFKISENLLKELENLLAKQDYLIK
FDDNIAQIMVEDAYSSNYGARPLKRWITKHLENEIAKLIIENRITKNINYKINYDRELDQVLIK

Sequences:

>Translated_704_residues
MEFKQKNSNNPLEEFGRNLNKEILDNKIDPIIGRDEEIRRTIEILSRKTKNNPVLIGEPGVGKTAIVEGLAYRIVHKDVP
SNLLDKTIIELSLSSLIAGASYQGQFEERINSILKEVKKADGNIILFIDEIHQIVGMGRNQGSNMDVANILKPMMARGEI
KLIGATTLDEYRLYIEKDQALERRFTKVLVNKPTIQETLTIMRGLKPR*EAFHGIKIHDNALIAAVNLSERYINDRNLPD
KAIDLIDEAAAKIKTQINSQPIYLDEIKRDLQHLQTEKAALEPEKDEKSIKRLNEILEKIKLKQDEFNNLNDLYLKEKKQ
IDDLKNLRQKIERIQHDIEFYQSEGKYEKASRLLYSDLPLFEKQREELENKINENNNQKMIIDSLTENEIADVIARATGI
PLSHLLADEKTKLLSLNKRIASHVIGQDEAVKLISDAVIRGRAGINNPNQPIGSFLFLGPTGVGKTELAKTLAKELFNSE
KALIRFDMSEYMEKHSVSKLVGAPPGYIGYENAGVLTESVKRKPYSILLFDEIEKAHPDILNILLQILDEGSIKDAKNNE
INFKNTIIIMTSNIGAEALLENNKTKALLDLQKTFKPEILNRISEIIFFNKLSKEIIFKISENLLKELENLLAKQDYLIK
FDDNIAQIMVEDAYSSNYGARPLKR*ITKHLENEIAKLIIENRITKNINYKINYDRELDQVLIK
>Mature_704_residues
MEFKQKNSNNPLEEFGRNLNKEILDNKIDPIIGRDEEIRRTIEILSRKTKNNPVLIGEPGVGKTAIVEGLAYRIVHKDVP
SNLLDKTIIELSLSSLIAGASYQGQFEERINSILKEVKKADGNIILFIDEIHQIVGMGRNQGSNMDVANILKPMMARGEI
KLIGATTLDEYRLYIEKDQALERRFTKVLVNKPTIQETLTIMRGLKPR*EAFHGIKIHDNALIAAVNLSERYINDRNLPD
KAIDLIDEAAAKIKTQINSQPIYLDEIKRDLQHLQTEKAALEPEKDEKSIKRLNEILEKIKLKQDEFNNLNDLYLKEKKQ
IDDLKNLRQKIERIQHDIEFYQSEGKYEKASRLLYSDLPLFEKQREELENKINENNNQKMIIDSLTENEIADVIARATGI
PLSHLLADEKTKLLSLNKRIASHVIGQDEAVKLISDAVIRGRAGINNPNQPIGSFLFLGPTGVGKTELAKTLAKELFNSE
KALIRFDMSEYMEKHSVSKLVGAPPGYIGYENAGVLTESVKRKPYSILLFDEIEKAHPDILNILLQILDEGSIKDAKNNE
INFKNTIIIMTSNIGAEALLENNKTKALLDLQKTFKPEILNRISEIIFFNKLSKEIIFKISENLLKELENLLAKQDYLIK
FDDNIAQIMVEDAYSSNYGARPLKR*ITKHLENEIAKLIIENRITKNINYKINYDRELDQVLIK

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=327, Percent_Identity=32.7217125382263, Blast_Score=184, Evalue=4e-46,
Organism=Escherichia coli, GI1788943, Length=704, Percent_Identity=47.4431818181818, Blast_Score=667, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=251, Percent_Identity=49.800796812749, Blast_Score=258, Evalue=7e-70,
Organism=Saccharomyces cerevisiae, GI6320464, Length=696, Percent_Identity=45.8333333333333, Blast_Score=633, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=699, Percent_Identity=42.346208869814, Blast_Score=539, Evalue=1e-154,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR001270
- InterPro:   IPR019489 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 80152; Mature: 80152

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFKQKNSNNPLEEFGRNLNKEILDNKIDPIIGRDEEIRRTIEILSRKTKNNPVLIGEPG
CCCCCCCCCCCHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCC
VGKTAIVEGLAYRIVHKDVPSNLLDKTIIELSLSSLIAGASYQGQFEERINSILKEVKKA
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
DGNIILFIDEIHQIVGMGRNQGSNMDVANILKPMMARGEIKLIGATTLDEYRLYIEKDQA
CCCEEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHH
LERRFTKVLVNKPTIQETLTIMRGLKPREAFHGIKIHDNALIAAVNLSERYINDRNLPDK
HHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHCCCEEECCEEEEEEECHHHHCCCCCCCHH
AIDLIDEAAAKIKTQINSQPIYLDEIKRDLQHLQTEKAALEPEKDEKSIKRLNEILEKIK
HHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LKQDEFNNLNDLYLKEKKQIDDLKNLRQKIERIQHDIEFYQSEGKYEKASRLLYSDLPLF
CCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHH
EKQREELENKINENNNQKMIIDSLTENEIADVIARATGIPLSHLLADEKTKLLSLNKRIA
HHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
SHVIGQDEAVKLISDAVIRGRAGINNPNQPIGSFLFLGPTGVGKTELAKTLAKELFNSEK
HHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCH
ALIRFDMSEYMEKHSVSKLVGAPPGYIGYENAGVLTESVKRKPYSILLFDEIEKAHPDIL
HEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCEEEEEHHHHHCCHHHH
NILLQILDEGSIKDAKNNEINFKNTIIIMTSNIGAEALLENNKTKALLDLQKTFKPEILN
HHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHCCCCHHHHHHHHHHCCHHHHH
RISEIIFFNKLSKEIIFKISENLLKELENLLAKQDYLIKFDDNIAQIMVEDAYSSNYGAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCC
PLKRITKHLENEIAKLIIENRITKNINYKINYDRELDQVLIK
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHCC
>Mature Secondary Structure
MEFKQKNSNNPLEEFGRNLNKEILDNKIDPIIGRDEEIRRTIEILSRKTKNNPVLIGEPG
CCCCCCCCCCCHHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCC
VGKTAIVEGLAYRIVHKDVPSNLLDKTIIELSLSSLIAGASYQGQFEERINSILKEVKKA
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
DGNIILFIDEIHQIVGMGRNQGSNMDVANILKPMMARGEIKLIGATTLDEYRLYIEKDQA
CCCEEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHH
LERRFTKVLVNKPTIQETLTIMRGLKPREAFHGIKIHDNALIAAVNLSERYINDRNLPDK
HHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHCCCEEECCEEEEEEECHHHHCCCCCCCHH
AIDLIDEAAAKIKTQINSQPIYLDEIKRDLQHLQTEKAALEPEKDEKSIKRLNEILEKIK
HHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
LKQDEFNNLNDLYLKEKKQIDDLKNLRQKIERIQHDIEFYQSEGKYEKASRLLYSDLPLF
CCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHH
EKQREELENKINENNNQKMIIDSLTENEIADVIARATGIPLSHLLADEKTKLLSLNKRIA
HHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
SHVIGQDEAVKLISDAVIRGRAGINNPNQPIGSFLFLGPTGVGKTELAKTLAKELFNSEK
HHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCH
ALIRFDMSEYMEKHSVSKLVGAPPGYIGYENAGVLTESVKRKPYSILLFDEIEKAHPDIL
HEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCEEEEEHHHHHCCHHHH
NILLQILDEGSIKDAKNNEINFKNTIIIMTSNIGAEALLENNKTKALLDLQKTFKPEILN
HHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHCCCCHHHHHHHHHHCCHHHHH
RISEIIFFNKLSKEIIFKISENLLKELENLLAKQDYLIKFDDNIAQIMVEDAYSSNYGAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCC
PLKRITKHLENEIAKLIIENRITKNINYKINYDRELDQVLIK
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7569993 [H]