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The map label for this gene is alaS

Identifier: 13357929

GI number: 13357929

Start: 420084

End: 422804

Strand: Direct

Name: alaS

Synonym: UU369

Alternate gene names: 13357929

Gene position: 420084-422804 (Clockwise)

Preceding gene: 13357928

Following gene: 13357930

Centisome position: 55.88

GC content: 27.16

Gene sequence:

>2721_bases
ATGCTGAAATTAAGTACGAATGAGATTAGAAAAAAGTGAATTGAATTTTTTGAGTCAAAGGATCATTTATTTATTGAACC
AAAGTCATTAATTCCCAAAAACGACCCTACACTATTATGAATTAATTCTGGTGTTTCAACCTTAAAAGATTATTTTAGTG
GTAAGGTTAAACCACCACATAAACGTTTAGTTAATTCTCAAAAAGCTATTCGTACAAATGATATTTTTAATGTTGGATTA
ACGAGCCGTCACCATACTTTTTTTGAAATGTTAGGGAATTTTTCAATTGGTGATTATTTTAAAAAAGAAGCTATTGATTG
AGCTTATGAATTTTTAATTAACGTTTTAAAAATTGATGTAAAAAAACTATGAGTAACTGTTTTTGAAGATGATCAATTTA
CGAATGATGAATGAATTAAATTAGGAATTATTAAAGAGCAAATTATTAAATGCAATCGTGATCGTAATTTTTGAGATGTA
GGTAATGGCCCTTGCGGCCCATGTACTGAAATTCATTATGATCGTGGTGAACGTTTTGACCCAAATAAAATAGGATCAAA
ATTAATTTTAGAAGATATTGAAAATGATCGTTATGTTGAAATTTGAAATATTGTTTTCTCACAATTTAATAATGATGGCC
ATAACAATTACACTGAATTATTACAAAAAAATATCGATACAGGAGCTGGCTTGGAAAGAATTGCTTGTATTTCACAAGAT
GTACCAACTAACTTTGATAGCGATGTTTTTATGCGAATTACAAAAAGTGTTGAACAGTTTTCAGAATATAAATATGACAT
GAATGAATACTTTCATCCCAATGTTACTCAAAATAAAATTAATTTCGCTTATAAAGTAATTGCAGATCATATGCGTGCAA
CTGTTTTTGCGATTGCAGATGGTGCAATTCCTTCAAATAAAGAACGAGGTTACATTTTACGTCGTTTAATTCGTCGAACT
ATGGTTTTAGTGCGTCGTTTAAATATTAACAATCTTTTATGAGTTGATGCAGTTGTTGACGCTATCGCTTCAACAATGGG
TGATTTTTATACTTATTTAAAAGATGAAAAAACACTTACTAAAATTAAAATGATTTTAAATAAAGAAGTGCAATTATTTG
AAAAAACTTTACAATTAGGTTTAAATATTTTTGAAAATTCTATTCGTAATCAAGAATTAGATAAAGAAATTACTTTTAAA
CTAGTTGATACATATGGTTTTCCAATTGAATTAATTAAAGAAATTTGTGAACAACGTAATGTTAAAGTTGATTTAGAAGC
TTTTGATGCTATGTTTAAACATCACCAATTAATTTCAAAAGCAAACAAAGCTAATTTAAAAGTAATGGAATCACAAAACG
AATCACTAATGCAATTAGATGTTGATTCAACCTTTCACTATGAAATATTTAGATGAGAAAATGCTAAAATCATTACTTTA
TTTAATGAGGATTTTGAATTGGTTGATGGTTTAGATCATGAAGATGGTTATGTTGTTTTTGATAACACATGTTTTTATGC
AACATCAGGTGGTCAACAACATGACACAGGTTATATTATTAAAAATGATCAACAATTTTTTGTTGATGATGTTTTTAAGG
CACCTAATCGTCAACACGTTCATCATGTTAAAAATGCTAGTTTATCAATGAATGAGCATGTTATTTTACAAATCAACGAG
CAAGATCGTAAATCAATTACAGCAAACCATACAGCCGAACACTTATTACACTATTGCTTAAAACATGTTTTAAGTCCTGA
TATTAAACAAGAAGGAGCTGCTAAATATCCACATAAAGTAACATTTGATTTTACTTATCATGCTCAACCAACAAAAGCAC
AATTAGATAAGTTAGAAAATGTATTAAATGAAATGGTACAAAGTAATTTTGATGTACAAGAATTACACATGGATTTAGAT
GAAGCTAAAGCTGTTGGTGCTGCTGCTTATTTTGAAGATGTTTATAAAAAATTAAAAGGCAAATTACGTGTAATTAAAAT
GGGACCTTCAATTGAGTTATGTGGGGGTACACACGCACATCATACATCAGAAATCGAGCGAATTAAAATTGTTGAATGTG
CTTCTAAAGGGGCTGGCTCTTGAAGAATTACTATGGTAACAGGTCATGATAATTTAGCAAAATACATTCATGATTTATAT
GTAGACTACTTAAATGAAATCAATCACTTAAAAGTTAATTTAGATATTAATGATCATAAATTAAATGATTTATATAATGC
TTTTGCAAATTGAAAAAATCTATCAATTGATGATTATGATTTATTAAATGAAAAATTTGCTGAACTAAAACAATCATTAA
TTAATTTTAAAATCGAATTTGATAAACAAAATGCCAAACAAGCTATTATTGATATTAAAAACACTTTTAATACACAACTA
ACTAATAAACGTGTACACGTTTTTAAAAACACTGATAATAAAAATATCTTCAATGCTTTAAATGAATTAATTAACGAAAA
TCAAGATACATTATTTATTAGTTTCAACTTAGATGATAATAAAATTCAATATTTATTAGCAATTAATGAAAAGTTTGCTA
CTACAAACCAAATTAATTTAAATAAATATATTAAAGAATTAAATACTATTTCTAATGGTAAAGGTGGGGGCAAGCCATAT
TTTGTTCAAGGTGGAACAAGTGAACAAGAAAAATTAGATGAGTTATTAACTGTTGTAGATAAATGAGTTATTAATGCGTA
A

Upstream 100 bases:

>100_bases
CCATAACAATGTAGTTTATTTAAAAACACCAAAACAAATTGCACCATCAATTATTAAAGTAATGCGTTAAATAATAAAAA
AGTAAGGAAGAATATTTATT

Downstream 100 bases:

>100_bases
ATTAGCACTTGATTTAGGAACAAAATCATGTGGTTTTGCAATTAGTGATTTATTAGGAATAATTGCTAGCGGACTTGATA
ATTTTATTTATGAAGAAAAT

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 906; Mature: 906

Protein sequence:

>906_residues
MLKLSTNEIRKKWIEFFESKDHLFIEPKSLIPKNDPTLLWINSGVSTLKDYFSGKVKPPHKRLVNSQKAIRTNDIFNVGL
TSRHHTFFEMLGNFSIGDYFKKEAIDWAYEFLINVLKIDVKKLWVTVFEDDQFTNDEWIKLGIIKEQIIKCNRDRNFWDV
GNGPCGPCTEIHYDRGERFDPNKIGSKLILEDIENDRYVEIWNIVFSQFNNDGHNNYTELLQKNIDTGAGLERIACISQD
VPTNFDSDVFMRITKSVEQFSEYKYDMNEYFHPNVTQNKINFAYKVIADHMRATVFAIADGAIPSNKERGYILRRLIRRT
MVLVRRLNINNLLWVDAVVDAIASTMGDFYTYLKDEKTLTKIKMILNKEVQLFEKTLQLGLNIFENSIRNQELDKEITFK
LVDTYGFPIELIKEICEQRNVKVDLEAFDAMFKHHQLISKANKANLKVMESQNESLMQLDVDSTFHYEIFRWENAKIITL
FNEDFELVDGLDHEDGYVVFDNTCFYATSGGQQHDTGYIIKNDQQFFVDDVFKAPNRQHVHHVKNASLSMNEHVILQINE
QDRKSITANHTAEHLLHYCLKHVLSPDIKQEGAAKYPHKVTFDFTYHAQPTKAQLDKLENVLNEMVQSNFDVQELHMDLD
EAKAVGAAAYFEDVYKKLKGKLRVIKMGPSIELCGGTHAHHTSEIERIKIVECASKGAGSWRITMVTGHDNLAKYIHDLY
VDYLNEINHLKVNLDINDHKLNDLYNAFANWKNLSIDDYDLLNEKFAELKQSLINFKIEFDKQNAKQAIIDIKNTFNTQL
TNKRVHVFKNTDNKNIFNALNELINENQDTLFISFNLDDNKIQYLLAINEKFATTNQINLNKYIKELNTISNGKGGGKPY
FVQGGTSEQEKLDELLTVVDKWVINA

Sequences:

>Translated_906_residues
MLKLSTNEIRKK*IEFFESKDHLFIEPKSLIPKNDPTLL*INSGVSTLKDYFSGKVKPPHKRLVNSQKAIRTNDIFNVGL
TSRHHTFFEMLGNFSIGDYFKKEAID*AYEFLINVLKIDVKKL*VTVFEDDQFTNDE*IKLGIIKEQIIKCNRDRNF*DV
GNGPCGPCTEIHYDRGERFDPNKIGSKLILEDIENDRYVEI*NIVFSQFNNDGHNNYTELLQKNIDTGAGLERIACISQD
VPTNFDSDVFMRITKSVEQFSEYKYDMNEYFHPNVTQNKINFAYKVIADHMRATVFAIADGAIPSNKERGYILRRLIRRT
MVLVRRLNINNLL*VDAVVDAIASTMGDFYTYLKDEKTLTKIKMILNKEVQLFEKTLQLGLNIFENSIRNQELDKEITFK
LVDTYGFPIELIKEICEQRNVKVDLEAFDAMFKHHQLISKANKANLKVMESQNESLMQLDVDSTFHYEIFR*ENAKIITL
FNEDFELVDGLDHEDGYVVFDNTCFYATSGGQQHDTGYIIKNDQQFFVDDVFKAPNRQHVHHVKNASLSMNEHVILQINE
QDRKSITANHTAEHLLHYCLKHVLSPDIKQEGAAKYPHKVTFDFTYHAQPTKAQLDKLENVLNEMVQSNFDVQELHMDLD
EAKAVGAAAYFEDVYKKLKGKLRVIKMGPSIELCGGTHAHHTSEIERIKIVECASKGAGS*RITMVTGHDNLAKYIHDLY
VDYLNEINHLKVNLDINDHKLNDLYNAFAN*KNLSIDDYDLLNEKFAELKQSLINFKIEFDKQNAKQAIIDIKNTFNTQL
TNKRVHVFKNTDNKNIFNALNELINENQDTLFISFNLDDNKIQYLLAINEKFATTNQINLNKYIKELNTISNGKGGGKPY
FVQGGTSEQEKLDELLTVVDK*VINA
>Mature_906_residues
MLKLSTNEIRKK*IEFFESKDHLFIEPKSLIPKNDPTLL*INSGVSTLKDYFSGKVKPPHKRLVNSQKAIRTNDIFNVGL
TSRHHTFFEMLGNFSIGDYFKKEAID*AYEFLINVLKIDVKKL*VTVFEDDQFTNDE*IKLGIIKEQIIKCNRDRNF*DV
GNGPCGPCTEIHYDRGERFDPNKIGSKLILEDIENDRYVEI*NIVFSQFNNDGHNNYTELLQKNIDTGAGLERIACISQD
VPTNFDSDVFMRITKSVEQFSEYKYDMNEYFHPNVTQNKINFAYKVIADHMRATVFAIADGAIPSNKERGYILRRLIRRT
MVLVRRLNINNLL*VDAVVDAIASTMGDFYTYLKDEKTLTKIKMILNKEVQLFEKTLQLGLNIFENSIRNQELDKEITFK
LVDTYGFPIELIKEICEQRNVKVDLEAFDAMFKHHQLISKANKANLKVMESQNESLMQLDVDSTFHYEIFR*ENAKIITL
FNEDFELVDGLDHEDGYVVFDNTCFYATSGGQQHDTGYIIKNDQQFFVDDVFKAPNRQHVHHVKNASLSMNEHVILQINE
QDRKSITANHTAEHLLHYCLKHVLSPDIKQEGAAKYPHKVTFDFTYHAQPTKAQLDKLENVLNEMVQSNFDVQELHMDLD
EAKAVGAAAYFEDVYKKLKGKLRVIKMGPSIELCGGTHAHHTSEIERIKIVECASKGAGS*RITMVTGHDNLAKYIHDLY
VDYLNEINHLKVNLDINDHKLNDLYNAFAN*KNLSIDDYDLLNEKFAELKQSLINFKIEFDKQNAKQAIIDIKNTFNTQL
TNKRVHVFKNTDNKNIFNALNELINENQDTLFISFNLDDNKIQYLLAINEKFATTNQINLNKYIKELNTISNGKGGGKPY
FVQGGTSEQEKLDELLTVVDK*VINA

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=964, Percent_Identity=29.5643153526971, Blast_Score=357, Evalue=2e-98,
Organism=Homo sapiens, GI38569417, Length=759, Percent_Identity=26.7457180500659, Blast_Score=262, Evalue=1e-69,
Organism=Escherichia coli, GI1789048, Length=904, Percent_Identity=32.7433628318584, Blast_Score=429, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17506981, Length=983, Percent_Identity=30.3153611393693, Blast_Score=356, Evalue=3e-98,
Organism=Caenorhabditis elegans, GI17536681, Length=760, Percent_Identity=28.6842105263158, Blast_Score=252, Evalue=7e-67,
Organism=Saccharomyces cerevisiae, GI6324911, Length=859, Percent_Identity=32.1303841676368, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24582809, Length=996, Percent_Identity=30.1204819277108, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI45552267, Length=996, Percent_Identity=30.1204819277108, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24658214, Length=795, Percent_Identity=27.0440251572327, Blast_Score=221, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 102741; Mature: 102741

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLSTNEIRKKIEFFESKDHLFIEPKSLIPKNDPTLLINSGVSTLKDYFSGKVKPPHKR
CCCCCHHHHHHHHHHHCCCCCEEECCHHCCCCCCCEEEEECCHHHHHHHHCCCCCCHHHH
LVNSQKAIRTNDIFNVGLTSRHHTFFEMLGNFSIGDYFKKEAIDAYEFLINVLKIDVKKL
HHCCCCCEECCCEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTVFEDDQFTNDEIKLGIIKEQIIKCNRDRNFDVGNGPCGPCTEIHYDRGERFDPNKIGS
HHHHCCCCCCCCCEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCC
KLILEDIENDRYVEINIVFSQFNNDGHNNYTELLQKNIDTGAGLERIACISQDVPTNFDS
HHHHHHCCCCCEEEEEEEEEECCCCCCCHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCC
DVFMRITKSVEQFSEYKYDMNEYFHPNVTQNKINFAYKVIADHMRATVFAIADGAIPSNK
HHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCC
ERGYILRRLIRRTMVLVRRLNINNLLVDAVVDAIASTMGDFYTYLKDEKTLTKIKMILNK
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
EVQLFEKTLQLGLNIFENSIRNQELDKEITFKLVDTYGFPIELIKEICEQRNVKVDLEAF
HHHHHHHHHHHHHHHHHHHHCCHHCCHHHEEEEEECCCCCHHHHHHHHHHCCCEEEHHHH
DAMFKHHQLISKANKANLKVMESQNESLMQLDVDSTFHYEIFRENAKIITLFNEDFELVD
HHHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCCCEEEEEEECCCEEEEEECCCHHHHC
GLDHEDGYVVFDNTCFYATSGGQQHDTGYIIKNDQQFFVDDVFKAPNRQHVHHVKNASLS
CCCCCCCEEEEECEEEEEECCCCCCCCCEEEECCCHHHHHHHHCCCCHHHHHHHCCCCCC
MNEHVILQINEQDRKSITANHTAEHLLHYCLKHVLSPDIKQEGAAKYPHKVTFDFTYHAQ
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHCCCCCCCCEEEEEEEECCC
PTKAQLDKLENVLNEMVQSNFDVQELHMDLDEAKAVGAAAYFEDVYKKLKGKLRVIKMGP
CHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
SIELCGGTHAHHTSEIERIKIVECASKGAGSRITMVTGHDNLAKYIHDLYVDYLNEINHL
CEEECCCCCCCCCHHHHHEEEEEHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCEE
KVNLDINDHKLNDLYNAFANKNLSIDDYDLLNEKFAELKQSLINFKIEFDKQNAKQAIID
EEEEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHH
IKNTFNTQLTNKRVHVFKNTDNKNIFNALNELINENQDTLFISFNLDDNKIQYLLAINEK
HHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEEECCC
FATTNQINLNKYIKELNTISNGKGGGKPYFVQGGTSEQEKLDELLTVVDKVINA
CCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLKLSTNEIRKKIEFFESKDHLFIEPKSLIPKNDPTLLINSGVSTLKDYFSGKVKPPHKR
CCCCCHHHHHHHHHHHCCCCCEEECCHHCCCCCCCEEEEECCHHHHHHHHCCCCCCHHHH
LVNSQKAIRTNDIFNVGLTSRHHTFFEMLGNFSIGDYFKKEAIDAYEFLINVLKIDVKKL
HHCCCCCEECCCEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTVFEDDQFTNDEIKLGIIKEQIIKCNRDRNFDVGNGPCGPCTEIHYDRGERFDPNKIGS
HHHHCCCCCCCCCEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCC
KLILEDIENDRYVEINIVFSQFNNDGHNNYTELLQKNIDTGAGLERIACISQDVPTNFDS
HHHHHHCCCCCEEEEEEEEEECCCCCCCHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCC
DVFMRITKSVEQFSEYKYDMNEYFHPNVTQNKINFAYKVIADHMRATVFAIADGAIPSNK
HHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCCC
ERGYILRRLIRRTMVLVRRLNINNLLVDAVVDAIASTMGDFYTYLKDEKTLTKIKMILNK
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
EVQLFEKTLQLGLNIFENSIRNQELDKEITFKLVDTYGFPIELIKEICEQRNVKVDLEAF
HHHHHHHHHHHHHHHHHHHHCCHHCCHHHEEEEEECCCCCHHHHHHHHHHCCCEEEHHHH
DAMFKHHQLISKANKANLKVMESQNESLMQLDVDSTFHYEIFRENAKIITLFNEDFELVD
HHHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCCCEEEEEEECCCEEEEEECCCHHHHC
GLDHEDGYVVFDNTCFYATSGGQQHDTGYIIKNDQQFFVDDVFKAPNRQHVHHVKNASLS
CCCCCCCEEEEECEEEEEECCCCCCCCCEEEECCCHHHHHHHHCCCCHHHHHHHCCCCCC
MNEHVILQINEQDRKSITANHTAEHLLHYCLKHVLSPDIKQEGAAKYPHKVTFDFTYHAQ
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHCCCCCCCCEEEEEEEECCC
PTKAQLDKLENVLNEMVQSNFDVQELHMDLDEAKAVGAAAYFEDVYKKLKGKLRVIKMGP
CHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECC
SIELCGGTHAHHTSEIERIKIVECASKGAGSRITMVTGHDNLAKYIHDLYVDYLNEINHL
CEEECCCCCCCCCHHHHHEEEEEHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCEE
KVNLDINDHKLNDLYNAFANKNLSIDDYDLLNEKFAELKQSLINFKIEFDKQNAKQAIID
EEEEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHH
IKNTFNTQLTNKRVHVFKNTDNKNIFNALNELINENQDTLFISFNLDDNKIQYLLAINEK
HHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEEECCC
FATTNQINLNKYIKELNTISNGKGGGKPYFVQGGTSEQEKLDELLTVVDKVINA
CCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA