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The map label for this gene is rpoD

Identifier: 13357909

GI number: 13357909

Start: 397484

End: 398830

Strand: Direct

Name: rpoD

Synonym: UU349

Alternate gene names: 13357909

Gene position: 397484-398830 (Clockwise)

Preceding gene: 13357908

Following gene: 13357910

Centisome position: 52.88

GC content: 29.99

Gene sequence:

>1347_bases
ATGTCAACAAAACAAAATGAACCATTATTTGTAAATCTAGAGGATTTAAAACAAAAAGTTAAATTATCATTTACTGAAGA
ATTCTCATTTTCGTATGCACTTTCTGAACTTGAAGCAGGAATTTTTGAAAGTCGTAATTTAAAAGGTGCAGATGCTGGTG
ATCCTGAAGAAATTTTATTAAATGTGGTTTTAGATGTTAGTAAGAAAAAACGTTCTCGAAATGAAATTAAATTTAATAAA
CTTCAAAATTATTTTATTCATATGAATTTACGTGAAGAACACTTTAGTGAAATTATTGATGTTCTAGAAGCTATTGGAAT
TCGTGTGCCTGATTATGATTTGGTGATGCAATCAAAATCAAAGACAAACACCAAAAAAAAGGATGAATATGGTATTGATG
ATACTTTAGAAATTAGCACATCAAAAATTGGTTTTTCATCAACAACAACCGAAAAAGTTGATGATGGTATTAAAGCTTAT
TTAGGAGTACTTGGAGAATCAAAAATGTTACGCTCTGATGAGGAAACTGAATATGCTAAAATGGTTATTAGTGACGATCC
ATCTCTAGTTAAAATTGGTAAAAATCAACTTTATACATCAAATATGCGTTTAGTTACTTCTATTGCTAAAAAATATTTAA
ATCGTGGATTAGACTTAGAAGATTTAATTCAAGAAGGGTCATCCGGTTTATTAAAAGCAATTGATAAATTTGATCATGAA
AAAGGACATAAATTTAGTACATATGCAACATGATGAATTCGCCAATCAATTACGCGCGCAATTGCTGATCAAGCTCGACA
AATTCGAATTCCTGTTCATATGGTTGAAACAATTAACAAATTAACTAAAGCCGAGCGAAATTTAATTCAAGAATTAGGAA
GGGATCCAACGGCTGAAGAAATTGCGCAAGCTATGAACAAAGTTTCACAAGCAAAAAATCAAAAAGAACAGTTAATCACT
GCACAAAAAGTTGTTGAGATTAAAAAATTAAATGTTGACCCAGTTTCATTAGATAAACAAATTGGTCATGATGAAGAATC
ACAATTTTCAGATTTCATTTCTGATGATGAAATTATTTCTCCAGAAAAATATACTGAAAAAAAAGCATTAAATGATCAGA
TTAATGAAATGTTTGAAAAAGTTTTAAACGATAATGAACAACGTGTTATTAAAATGCGGTATGGTTTATTACCCTTCGAA
CGTCCTTATACACTTGAAGAAGTTGGTGAATATTTAGGAGTTACACGCGAACGTGCACGTCAAATTGAATCAAAAGCAAT
TCGTAAATTAAAACATCCATCAAAAACCGCAAAACTACGTTCATTTATTGGTGAATCCGAAAACTAG

Upstream 100 bases:

>100_bases
TTTGGAGAAAAATAAGAAATATTAAAAAATAAACACTGAAGTGTTTATTTTTTAATTATGTTATAATTAAAATTAAAATA
TGAGCGATGAGGATATTTAG

Downstream 100 bases:

>100_bases
AATTGCTTTTATTGCTGATTTAATTAATGACGCTCAAAATGTTGTCGATGTTGGTAGTGATCACGCATATTTAGCTAAAA
TCTTATTGGTTAACAATAAA

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-A [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MSTKQNEPLFVNLEDLKQKVKLSFTEEFSFSYALSELEAGIFESRNLKGADAGDPEEILLNVVLDVSKKKRSRNEIKFNK
LQNYFIHMNLREEHFSEIIDVLEAIGIRVPDYDLVMQSKSKTNTKKKDEYGIDDTLEISTSKIGFSSTTTEKVDDGIKAY
LGVLGESKMLRSDEETEYAKMVISDDPSLVKIGKNQLYTSNMRLVTSIAKKYLNRGLDLEDLIQEGSSGLLKAIDKFDHE
KGHKFSTYATWWIRQSITRAIADQARQIRIPVHMVETINKLTKAERNLIQELGRDPTAEEIAQAMNKVSQAKNQKEQLIT
AQKVVEIKKLNVDPVSLDKQIGHDEESQFSDFISDDEIISPEKYTEKKALNDQINEMFEKVLNDNEQRVIKMRYGLLPFE
RPYTLEEVGEYLGVTRERARQIESKAIRKLKHPSKTAKLRSFIGESEN

Sequences:

>Translated_448_residues
MSTKQNEPLFVNLEDLKQKVKLSFTEEFSFSYALSELEAGIFESRNLKGADAGDPEEILLNVVLDVSKKKRSRNEIKFNK
LQNYFIHMNLREEHFSEIIDVLEAIGIRVPDYDLVMQSKSKTNTKKKDEYGIDDTLEISTSKIGFSSTTTEKVDDGIKAY
LGVLGESKMLRSDEETEYAKMVISDDPSLVKIGKNQLYTSNMRLVTSIAKKYLNRGLDLEDLIQEGSSGLLKAIDKFDHE
KGHKFSTYAT**IRQSITRAIADQARQIRIPVHMVETINKLTKAERNLIQELGRDPTAEEIAQAMNKVSQAKNQKEQLIT
AQKVVEIKKLNVDPVSLDKQIGHDEESQFSDFISDDEIISPEKYTEKKALNDQINEMFEKVLNDNEQRVIKMRYGLLPFE
RPYTLEEVGEYLGVTRERARQIESKAIRKLKHPSKTAKLRSFIGESEN
>Mature_447_residues
STKQNEPLFVNLEDLKQKVKLSFTEEFSFSYALSELEAGIFESRNLKGADAGDPEEILLNVVLDVSKKKRSRNEIKFNKL
QNYFIHMNLREEHFSEIIDVLEAIGIRVPDYDLVMQSKSKTNTKKKDEYGIDDTLEISTSKIGFSSTTTEKVDDGIKAYL
GVLGESKMLRSDEETEYAKMVISDDPSLVKIGKNQLYTSNMRLVTSIAKKYLNRGLDLEDLIQEGSSGLLKAIDKFDHEK
GHKFSTYAT**IRQSITRAIADQARQIRIPVHMVETINKLTKAERNLIQELGRDPTAEEIAQAMNKVSQAKNQKEQLITA
QKVVEIKKLNVDPVSLDKQIGHDEESQFSDFISDDEIISPEKYTEKKALNDQINEMFEKVLNDNEQRVIKMRYGLLPFER
PYTLEEVGEYLGVTRERARQIESKAIRKLKHPSKTAKLRSFIGESEN

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=251, Percent_Identity=48.605577689243, Blast_Score=235, Evalue=3e-63,
Organism=Escherichia coli, GI1789098, Length=296, Percent_Identity=37.8378378378378, Blast_Score=191, Evalue=9e-50,
Organism=Escherichia coli, GI1789871, Length=289, Percent_Identity=27.681660899654, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 50980; Mature: 50848

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTKQNEPLFVNLEDLKQKVKLSFTEEFSFSYALSELEAGIFESRNLKGADAGDPEEILL
CCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
NVVLDVSKKKRSRNEIKFNKLQNYFIHMNLREEHFSEIIDVLEAIGIRVPDYDLVMQSKS
HHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHCCCCH
KTNTKKKDEYGIDDTLEISTSKIGFSSTTTEKVDDGIKAYLGVLGESKMLRSDEETEYAK
HCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHH
MVISDDPSLVKIGKNQLYTSNMRLVTSIAKKYLNRGLDLEDLIQEGSSGLLKAIDKFDHE
HEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHC
KGHKFSTYATIRQSITRAIADQARQIRIPVHMVETINKLTKAERNLIQELGRDPTAEEIA
CCCCHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
QAMNKVSQAKNQKEQLITAQKVVEIKKLNVDPVSLDKQIGHDEESQFSDFISDDEIISPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHCCCCCCCCCH
KYTEKKALNDQINEMFEKVLNDNEQRVIKMRYGLLPFERPYTLEEVGEYLGVTRERARQI
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHH
ESKAIRKLKHPSKTAKLRSFIGESEN
HHHHHHHHHCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
STKQNEPLFVNLEDLKQKVKLSFTEEFSFSYALSELEAGIFESRNLKGADAGDPEEILL
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
NVVLDVSKKKRSRNEIKFNKLQNYFIHMNLREEHFSEIIDVLEAIGIRVPDYDLVMQSKS
HHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHCCCCH
KTNTKKKDEYGIDDTLEISTSKIGFSSTTTEKVDDGIKAYLGVLGESKMLRSDEETEYAK
HCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHH
MVISDDPSLVKIGKNQLYTSNMRLVTSIAKKYLNRGLDLEDLIQEGSSGLLKAIDKFDHE
HEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHC
KGHKFSTYATIRQSITRAIADQARQIRIPVHMVETINKLTKAERNLIQELGRDPTAEEIA
CCCCHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
QAMNKVSQAKNQKEQLITAQKVVEIKKLNVDPVSLDKQIGHDEESQFSDFISDDEIISPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHHHCCCCCCCCCH
KYTEKKALNDQINEMFEKVLNDNEQRVIKMRYGLLPFERPYTLEEVGEYLGVTRERARQI
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHH
ESKAIRKLKHPSKTAKLRSFIGESEN
HHHHHHHHHCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7569993; 1945886 [H]