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The map label for this gene is pcrA [H]

Identifier: 126698622

GI number: 126698622

Start: 1221251

End: 1223470

Strand: Direct

Name: pcrA [H]

Synonym: CD1038

Alternate gene names: 126698622

Gene position: 1221251-1223470 (Clockwise)

Preceding gene: 126698621

Following gene: 126698623

Centisome position: 28.47

GC content: 27.16

Gene sequence:

>2220_bases
ATGATTAATATAAATTATAGAGAAGACCAGATACCAATAATAAATTACGATAATGGTACTATGGCAGTACCAGCAGTACC
AGGTGCTGGAAAAACATTTATAATAACAAATTTAGTAGCTAAACTATTACTTGAACAGAAACATAAGGGTGGTAAAATAT
TAATTCTTACATATATGAATAGTGCTGTAAATAATTTTAAAGGTAGAATAAGAAAGATTCTTGAAGAGAATAAAATAGAT
GATACTAATTCGTATGAAGTAATGACTATACACAGTTTGGCTGTAAAAATAATAAAGGAAAAGCCAGAAGTTGTAATGTT
AAGTGAGGATTTTAACATAGCTGACGATTTGCAAAAAAGTATTATATTAAATGAATGTATAAGTAGATTTAGATTTGAAG
GTGGAGAAAGAGCTTTTAGATGGTTTTTAAAGGAGCAAAAAGATGAACGCTGGAAAGAAATAACTCTTGAAGCCTGGGAA
AGAGGTTTTTTTGAATTTGCAGGAAATGCAATAAGTGAACTTAAGTATAAGGGAATTTCTCCAGATAAACTTGAAGAAAT
ATTGTCAAGAGGACACAAAGGAATGCTTAAAATTATTTTACCAATATATAAATTGTATGATAGAAAATTAAAGCAAAATG
GATTGCTAGATTATGATGATATATTAATTTTAGCAGAAAAAACTTTAAGCTTAGACGAAGGGCTTAGAAAGAAGTTTCAA
ACTAGATACAAATATATATTTGAAGATGAGTGTCAGGACTCAAATGAAATTCAAGGTAATATAATAAAAATTATATCTAG
CGAAAATAAAAACCTAGTTAGAGTTGGAGATATAAATCAGAGTATAACAGGAACATTTTCATCATCAGACCCTAAATTTT
TTAAAGAATTTATAAATAGTGCAGATTTCTGTTATAGGATGGATATGTCAAATCGAAGTTCAAAAGATATACTTGATTTA
GCAAACACTCTTGTCAAGTATGTTACAAAGGAATTTAGACAGAAAGAATGTAGACATGCTCTTGAAGATATGCAAATAAA
AACTGTGCCAAATGGAATGGGATATAAAGAAAACCCTAATCCAGAACATTATAATATAAATGTAAAGTGGTATGAGAGTT
GGAAAATTGAAATAGAGCAAACTGCTAGATACGTTAAGGGTATAAAAAATAAATATCCAGATAAAAGTATAGGTATATTG
GTTCCATTTAATGAACAAGTGACACAAGTTGCTAAAGAACTTAGAGAGAATAATCTAACCTTTGAAGAATTAGGACCAAA
TTCTTTGAATAAAAGGAAGGTTATAAACAACATAGCATATATTATTGATTTTATGTTGCATTGTGATGATGTAGAAAAGT
TAATAGTTGTACTTAATAAAGTTTTTATAGATACTGATAATGAAGAAGAAATAAAGTATTTTTTAGAAGAATTAAAAAAC
TATTCTACCGAAGAAATTATTTATAATTTTGAATTTAGTAAGTTAGATAATGATTTAATAGAATTTAAAGAAGAAAGTGA
TATATACTTAAGTTTTAAAAGAGGAATAGATATTATAAAACAGATATTAGAATATCCAATAATAAGGTTAGACCTCTTAG
TTTTGTTTATTGGGAAAAAACTTAACTTAGAAAAAGAAGATAAAGCTGTACTGGATTATATATCTTTTTATATAAAGTAT
CTAGTATCAGAAAATATAAATATGGATTTAGAAGATGTATATAATTTATTGTTTGATGTAAAGAACAGGGTGTTTAATCA
TATAATAGATGTGGTATATGAGATAAATGGATATGAACCAGAGCCTGGTAGTATAACGCTATGTAACTATCATAAATCAA
AGGGGATGGAATGGGATTGTGTGTTTTTACTAGGTCTTGTTGAATATAATTTCCCTGACAATATTAATCAAAAGTTTCAG
AGTGATAAATGGTATCTAAAAGAAAAATATAAAAACCCTATGGCAATTATTAAATCAGAAGTTGAAGCTATATTAAAAGG
AAGTATTTCAACAGATTATGCTCATAAAACGAAGATTGATTCTATAAATGAAAAGATTAGACTTTTATATGTAGGGATTA
CTAGAGCAAAGGAAATGTTAGTACTTTCAGGAAGTGCTTATAGAGATGAAAGTGATATAGGGAATAAGAGAAAAGAGCAA
AAACCATGTATATATTTAAGCAGACTTAATCAACATATTATTGAAAAAAGGTCAAATTAA

Upstream 100 bases:

>100_bases
ATCTATGCATTTAAAAGTGAATATGCGATTAATGGTTATATCCAAGAGAGTATATTATATAGTATACTTTTAAAAATCTC
ACATAAAGGAGATAAAAATT

Downstream 100 bases:

>100_bases
TATATAATAAGTTATTAAAAGATTGCTTGAAATATATCTAAAAGGGGATAAAACAATATGTACAGTAAGTAACTACTAAA
AAATAAAGTTAAGAGAGAAG

Product: helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 739; Mature: 739

Protein sequence:

>739_residues
MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLLEQKHKGGKILILTYMNSAVNNFKGRIRKILEENKID
DTNSYEVMTIHSLAVKIIKEKPEVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDYDDILILAEKTLSLDEGLRKKFQ
TRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVGDINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDL
ANTLVKYVTKEFRQKECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKYPDKSIGIL
VPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDDVEKLIVVLNKVFIDTDNEEEIKYFLEELKN
YSTEEIIYNFEFSKLDNDLIEFKEESDIYLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKY
LVSENINMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLLGLVEYNFPDNINQKFQ
SDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSINEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQ
KPCIYLSRLNQHIIEKRSN

Sequences:

>Translated_739_residues
MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLLEQKHKGGKILILTYMNSAVNNFKGRIRKILEENKID
DTNSYEVMTIHSLAVKIIKEKPEVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDYDDILILAEKTLSLDEGLRKKFQ
TRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVGDINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDL
ANTLVKYVTKEFRQKECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKYPDKSIGIL
VPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDDVEKLIVVLNKVFIDTDNEEEIKYFLEELKN
YSTEEIIYNFEFSKLDNDLIEFKEESDIYLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKY
LVSENINMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLLGLVEYNFPDNINQKFQ
SDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSINEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQ
KPCIYLSRLNQHIIEKRSN
>Mature_739_residues
MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLLEQKHKGGKILILTYMNSAVNNFKGRIRKILEENKID
DTNSYEVMTIHSLAVKIIKEKPEVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDYDDILILAEKTLSLDEGLRKKFQ
TRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVGDINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDL
ANTLVKYVTKEFRQKECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKYPDKSIGIL
VPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDDVEKLIVVLNKVFIDTDNEEEIKYFLEELKN
YSTEEIIYNFEFSKLDNDLIEFKEESDIYLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKY
LVSENINMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLLGLVEYNFPDNINQKFQ
SDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSINEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQ
KPCIYLSRLNQHIIEKRSN

Specific function: Essential helicase. May act as a helicase in plasmid pT181 replication [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=457, Percent_Identity=23.6323851203501, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6322369, Length=468, Percent_Identity=23.0769230769231, Blast_Score=89, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 86541; Mature: 86541

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLLEQKHKGGKILILTYMN
CEECCCCCCCCCEEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEHH
SAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKPEVVMLSEDFNIADDLQKS
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHCCCCEEEEECCCCHHHHHHHH
IILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWERGFFEFAGNAISELKYKGIS
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCC
PDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDYDDILILAEKTLSLDEGLRKKFQ
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHCCHHHHHHHHHH
TRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVGDINQSITGTFSSSDPKFFKEFINS
HHHHHHCCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCEECCCCCCHHHHHHHHCC
ADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQKECRHALEDMQIKTVPNGMGYKENPN
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCC
PEHYNINVKWYESWKIEIEQTARYVKGIKNKYPDKSIGILVPFNEQVTQVAKELRENNLT
CCEEEEEEEEEECEEEEHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHCCCC
FEELGPNSLNKRKVINNIAYIIDFMLHCDDVEKLIVVLNKVFIDTDNEEEIKYFLEELKN
HHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
YSTEEIIYNFEFSKLDNDLIEFKEESDIYLSFKRGIDIIKQILEYPIIRLDLLVLFIGKK
CCCHHEEEEEEHHHCCCHHHHHCCCCCEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHCCC
LNLEKEDKAVLDYISFYIKYLVSENINMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEP
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EPGSITLCNYHKSKGMEWDCVFLLGLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSE
CCCCEEEEEEHHCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHCCHHHHHHHH
VEAILKGSISTDYAHKTKIDSINEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQ
HHHHHHCCCCCCCHHHHHHHHHCCEEEEEEEEHHHCCEEEEECCCCCCCCHHCCCCCHHC
KPCIYLSRLNQHIIEKRSN
CCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLLEQKHKGGKILILTYMN
CEECCCCCCCCCEEEECCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEHH
SAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKPEVVMLSEDFNIADDLQKS
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHCCCCEEEEECCCCHHHHHHHH
IILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWERGFFEFAGNAISELKYKGIS
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCC
PDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDYDDILILAEKTLSLDEGLRKKFQ
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHCCHHHHHHHHHH
TRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVGDINQSITGTFSSSDPKFFKEFINS
HHHHHHCCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCEECCCCCCHHHHHHHHCC
ADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQKECRHALEDMQIKTVPNGMGYKENPN
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCC
PEHYNINVKWYESWKIEIEQTARYVKGIKNKYPDKSIGILVPFNEQVTQVAKELRENNLT
CCEEEEEEEEEECEEEEHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHCCCC
FEELGPNSLNKRKVINNIAYIIDFMLHCDDVEKLIVVLNKVFIDTDNEEEIKYFLEELKN
HHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
YSTEEIIYNFEFSKLDNDLIEFKEESDIYLSFKRGIDIIKQILEYPIIRLDLLVLFIGKK
CCCHHEEEEEEHHHCCCHHHHHCCCCCEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHCCC
LNLEKEDKAVLDYISFYIKYLVSENINMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEP
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EPGSITLCNYHKSKGMEWDCVFLLGLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSE
CCCCEEEEEEHHCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHCCHHHHHHHH
VEAILKGSISTDYAHKTKIDSINEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQ
HHHHHHCCCCCCCHHHHHHHHHCCEEEEEEEEHHHCCEEEEECCCCCCCCHHCCCCCHHC
KPCIYLSRLNQHIIEKRSN
CCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8232203 [H]