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The map label for this gene is yyaE [H]

Identifier: 126698349

GI number: 126698349

Start: 940287

End: 942431

Strand: Direct

Name: yyaE [H]

Synonym: CD0769

Alternate gene names: 126698349

Gene position: 940287-942431 (Clockwise)

Preceding gene: 126698348

Following gene: 126698350

Centisome position: 21.92

GC content: 28.62

Gene sequence:

>2145_bases
ATGGATATAATAAAAAAATTAAGTCATGGGTGTACATTAGATTGTCATGACTGTTGTAAATTCAATGTTTATACTAAAGG
AAATAATGTAGTAAAAATTGAAGGAGATAAAAATCATCCTTATACAAAAGGTTTTATATGCAAAAAAGGGATGGCCCATT
TAGATAGATTAAATCATAAAGATAGAATAAAGACTCCTATGCTTAAAGTTGATGGAGTATGGAAAGAGATTTCTTTTGAT
AAAGCTATAGAAATAATGGCAGAAAAGCTTACATACTATAAAGAAAAGTATACATCAAAGTCTGTCATGCATTATGACCA
ATATGGAAGTGGGTCTGTGCTAAAATACATAGGAGATATATTTTTTAATTTTTATGGAGGTGTTAGCAGACATAAAGGTG
GTCCCTGTTGGAGTGCAGGGATGCATGCTCAAAAATATGATTTTGGAGTAGCTAAGAGTCATGCTATAGAAGATATGTTA
AATAGTAAAAGTATATTTGTGTGGGGAAAAAATCCAGCATATACTACAATTCATACTATGCAAATAATTAAAAAAGCTAA
AGAAAAAGGGATAAAGATAGTAGTGATTGACCCCATATATACAAAAACAGCTCAAATAGCAGATAAGTATGTACAAGTAA
ATCCTGGAACAGATGGAGCTTTAGCTATAGCTATGGCAAAAATTATAGTAGAAGATAAATTATATGATGAAGAATATATA
AACTCGTATGTTATTGGTTTTGAAGAATATAAAAAATATTTATCTTCATTGGAATTAAGTTTTTTAATTGATGAATGTGG
TGTTAAAGAGAATGATATAAGAGAGTTAGTGGATTTATATACTAACAAATATTCAAGTATAAATGTTGGATATGGGCTTC
AAAAATATAAAAATGGTGGAAACACAATAAGAGCAATAGATGCTTTAGGTGCTATAACTGGTCAAATAGGATTTAGTGGT
GGAGGGGTAAACTATGCTAATAAAGTTTATCCGAGTGTTATAAATTCTGACCCTTACAATAGCCAATCATATGGTGAAGA
TAGGGAGTTTTATGTAAGTAATATCAGTAAATTTATAGAAGAATCTTTAAAAAATACATCAAATAAAGTAAATTATGCAT
CAGATGAACTTGATATGACATCAAATAAAGTAAATTATGTATCAGATGAACTTGATATAACATCAAATAAAACAGATTAT
ATATCAAATGAATTGTATAATTTATCTAATAAGTCAATCAAAGGTAATATTCCTATAAAGATGGCTGTAATAACTAAAAG
TAATATGCTGAATCAACTGCCAGATTTAGTAGAATTGGAGAGGGTGTTTTCAAAAATTGAATTTAAAGTATGTTTTGATA
TGTTTATAACTGATACAGCTACTTTATGTGACATGTTTATACCGTGTACAAATACACTAGAGAGTGAAGATATAATTTAT
AGCTCAATGACCAATCCATATATAACCTATAATGAAAGAGCTGTAAAGCCAGCACATAAATTGATGGATGAATATTACTT
CTTTATGGAGCTAGCTAAAAAGATGGGATTAAATGATTATCCTTTTGTAGAAAAGAGAACTTATTTAGAAAAAGTGATTG
AACCACTTAAGCGTTTTGACAAAAATCTGGATATAGAAAAACTTAAAAACAATTACTTTACTATACATAACCCTGTTGCT
TGGGAAGATAAGAAGTTTGAGACTCCATCTGGAAAATACGAGCTTTATTCAGAAAGTATAAAAAACTTGGGAATAAGTCC
AACACCTGTATATATAAGCAATAAATATAAAGAAATAGAAGATAAAAATATTTCTTTTAGATTATTAACTAATCATCATG
CAGATACTTTATTTAGTCAGCATTTTATGGACAAAAAATCTATAGCTCAAGCATATATAAATCGGAGAATGGCTAAAAAA
GTAGGTATTGAGGACAAAGATATTGTGATATTGAGGTCAAAAAAAGCAAAAATAAATGTACAAATAAATATAGATGATGG
AGTAGGTAATTATATAGTAAAAATGTATGTTGGTTGGTGGAAGAAACATGGAAATCCTAATAGTTTAACAGACACTGGTA
TATCAGATTTTGGGGGTCAGGTAACATATAATGAAAGTATGGTAGAAATAATACGACAAAACTAG

Upstream 100 bases:

>100_bases
TATGTTTTATATACTGTAGTTTTGAACATAGAAAGTTTAATAAATTATAGTATATATGTTAATTTTTAATAAGGTAAGAA
AGTAGAAAGAAGGATATTTT

Downstream 100 bases:

>100_bases
TTAAATTTCATTGAAGGAATAAAAATATAATTATAGAATTGATTAAAAGTTACTATTTTTACAAAACTATTGAAGGAGGG
ATTTTTGATGGTAAATTATT

Product: formate dehydrogenase

Products: CO2; NADH; H2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 714; Mature: 714

Protein sequence:

>714_residues
MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKGFICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFD
KAIEIMAEKLTYYKEKYTSKSVMHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTDGALAIAMAKIIVEDKLYDEEYI
NSYVIGFEEYKKYLSSLELSFLIDECGVKENDIRELVDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSG
GGVNYANKVYPSVINSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDMTSNKVNYVSDELDITSNKTDY
ISNELYNLSNKSIKGNIPIKMAVITKSNMLNQLPDLVELERVFSKIEFKVCFDMFITDTATLCDMFIPCTNTLESEDIIY
SSMTNPYITYNERAVKPAHKLMDEYYFFMELAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNPVA
WEDKKFETPSGKYELYSESIKNLGISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMDKKSIAQAYINRRMAKK
VGIEDKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKKHGNPNSLTDTGISDFGGQVTYNESMVEIIRQN

Sequences:

>Translated_714_residues
MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKGFICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFD
KAIEIMAEKLTYYKEKYTSKSVMHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTDGALAIAMAKIIVEDKLYDEEYI
NSYVIGFEEYKKYLSSLELSFLIDECGVKENDIRELVDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSG
GGVNYANKVYPSVINSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDMTSNKVNYVSDELDITSNKTDY
ISNELYNLSNKSIKGNIPIKMAVITKSNMLNQLPDLVELERVFSKIEFKVCFDMFITDTATLCDMFIPCTNTLESEDIIY
SSMTNPYITYNERAVKPAHKLMDEYYFFMELAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNPVA
WEDKKFETPSGKYELYSESIKNLGISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMDKKSIAQAYINRRMAKK
VGIEDKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKKHGNPNSLTDTGISDFGGQVTYNESMVEIIRQN
>Mature_714_residues
MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKGFICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFD
KAIEIMAEKLTYYKEKYTSKSVMHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTDGALAIAMAKIIVEDKLYDEEYI
NSYVIGFEEYKKYLSSLELSFLIDECGVKENDIRELVDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSG
GGVNYANKVYPSVINSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDMTSNKVNYVSDELDITSNKTDY
ISNELYNLSNKSIKGNIPIKMAVITKSNMLNQLPDLVELERVFSKIEFKVCFDMFITDTATLCDMFIPCTNTLESEDIIY
SSMTNPYITYNERAVKPAHKLMDEYYFFMELAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNPVA
WEDKKFETPSGKYELYSESIKNLGISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMDKKSIAQAYINRRMAKK
VGIEDKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKKHGNPNSLTDTGISDFGGQVTYNESMVEIIRQN

Specific function: Decomposes Formic Acid To Hydrogen And Carbon Dioxide Under Anaerobic Conditions In The Absence Of Exogenous Electron Acceptors. [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI3868721, Length=703, Percent_Identity=25.74679943101, Blast_Score=179, Evalue=6e-46,
Organism=Escherichia coli, GI1787870, Length=755, Percent_Identity=24.5033112582781, Blast_Score=178, Evalue=1e-45,
Organism=Escherichia coli, GI171474008, Length=756, Percent_Identity=24.3386243386243, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI87081797, Length=769, Percent_Identity=23.0169050715215, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI87081994, Length=744, Percent_Identity=22.0430107526882, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI1787231, Length=758, Percent_Identity=22.0316622691293, Blast_Score=116, Evalue=6e-27,
Organism=Escherichia coli, GI145693196, Length=716, Percent_Identity=20.5307262569832, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI3868720, Length=399, Percent_Identity=23.3082706766917, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.2.1.2 [C]

Molecular weight: Translated: 81684; Mature: 81684

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKGFICKKGMAHLDRLNHK
CCHHHHHHCCCCEEHHHCCEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCC
DRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSVMHYDQYGSGSVLKYIGDI
HHHCCCCEEECCCHHHCCHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCHHHHHHHHH
FFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDMLNSKSIFVWGKNPAYTTIHTM
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHH
QIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTDGALAIAMAKIIVEDKLYDEEYI
HHHHHHHHCCCEEEEECCCHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHCCHHHH
NSYVIGFEEYKKYLSSLELSFLIDECGVKENDIRELVDLYTNKYSSINVGYGLQKYKNGG
HHHEECHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEECCCCHHHHCCCC
NTIRAIDALGAITGQIGFSGGGVNYANKVYPSVINSDPYNSQSYGEDREFYVSNISKFIE
CEEEHHHHHHHHHCCCCCCCCCCCCHHHHCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
ESLKNTSNKVNYASDELDMTSNKVNYVSDELDITSNKTDYISNELYNLSNKSIKGNIPIK
HHHHCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHCCCCCCEECCCCEE
MAVITKSNMLNQLPDLVELERVFSKIEFKVCFDMFITDTATLCDMFIPCTNTLESEDIIY
EEEEECHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
SSMTNPYITYNERAVKPAHKLMDEYYFFMELAKKMGLNDYPFVEKRTYLEKVIEPLKRFD
ECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
KNLDIEKLKNNYFTIHNPVAWEDKKFETPSGKYELYSESIKNLGISPTPVYISNKYKEIE
CCCCHHHHCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCHHCC
DKNISFRLLTNHHADTLFSQHFMDKKSIAQAYINRRMAKKVGIEDKDIVILRSKKAKINV
CCCCEEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEE
QINIDDGVGNYIVKMYVGWWKKHGNPNSLTDTGISDFGGQVTYNESMVEIIRQN
EEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCEEEECHHHHHHHHCC
>Mature Secondary Structure
MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKGFICKKGMAHLDRLNHK
CCHHHHHHCCCCEEHHHCCEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCC
DRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSVMHYDQYGSGSVLKYIGDI
HHHCCCCEEECCCHHHCCHHHHHHHHHHHHHHHHHHHCCCHHEEECCCCCCHHHHHHHHH
FFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDMLNSKSIFVWGKNPAYTTIHTM
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHH
QIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTDGALAIAMAKIIVEDKLYDEEYI
HHHHHHHHCCCEEEEECCCHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHCCHHHH
NSYVIGFEEYKKYLSSLELSFLIDECGVKENDIRELVDLYTNKYSSINVGYGLQKYKNGG
HHHEECHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEECCCCHHHHCCCC
NTIRAIDALGAITGQIGFSGGGVNYANKVYPSVINSDPYNSQSYGEDREFYVSNISKFIE
CEEEHHHHHHHHHCCCCCCCCCCCCHHHHCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
ESLKNTSNKVNYASDELDMTSNKVNYVSDELDITSNKTDYISNELYNLSNKSIKGNIPIK
HHHHCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHCCCCCCEECCCCEE
MAVITKSNMLNQLPDLVELERVFSKIEFKVCFDMFITDTATLCDMFIPCTNTLESEDIIY
EEEEECHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
SSMTNPYITYNERAVKPAHKLMDEYYFFMELAKKMGLNDYPFVEKRTYLEKVIEPLKRFD
ECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
KNLDIEKLKNNYFTIHNPVAWEDKKFETPSGKYELYSESIKNLGISPTPVYISNKYKEIE
CCCCHHHHCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCHHCC
DKNISFRLLTNHHADTLFSQHFMDKKSIAQAYINRRMAKKVGIEDKDIVILRSKKAKINV
CCCCEEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEE
QINIDDGVGNYIVKMYVGWWKKHGNPNSLTDTGISDFGGQVTYNESMVEIIRQN
EEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCEEEECHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: Fe; Mo; Se [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.19 {3-pyridinecarboxaldehyde-NAD+}} 0.032 {NAD+}} 1.16 {3-acetylpyridine-NAD+}} 0.54 {deamino-NAD+}} 0.17 {thio-NAD+}} [C]

Substrates: Formate; NAD(+) [C]

Specific reaction: Formate + NAD(+) = CO2 + NADH. formate = CO2 + H2 [C]

General reaction: Redox reaction [C]

Inhibitor: Azide; Br-; Cl-; CN-; F-; HCO3-; HCOS-; NO2-; NO3-; OCN-; SCN- [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 1552862 [H]