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The map label for this gene is pyrF

Identifier: 126696852

GI number: 126696852

Start: 1281605

End: 1282333

Strand: Reverse

Name: pyrF

Synonym: P9301_15141

Alternate gene names: 126696852

Gene position: 1282333-1281605 (Counterclockwise)

Preceding gene: 126696853

Following gene: 126696845

Centisome position: 78.1

GC content: 30.18

Gene sequence:

>729_bases
ATGAATAAAAGATTTAATTCAGAAGATAAAATAATATTGGCAATTGATGGATTAGATGTAAGTCAAGCAAAATTACTTCT
GGAAAAATGTCCTGATATTAAGTGGGTGAAAATTGGTTTAGAGCTTTTTGTTAGGGAAGGTCCAAGAGTTATTGAAATAT
TAAAAGGTTTAAATAAAAAAATTTTTTTAGACTTAAAATTTCATGATATTCCAAATACCATGCGTGCAGCATGTTTCCAA
GTCTCAAAATTAGGAGTTGATATAATCTCTATTCATTCTTCAGCAGGTCTAAAAGCCCTTAAGGAATCGAAGAAAGCCTC
TTTAGAAGGAGCCAAATCATTAGGTGTTAAGCCTCCATTTGTTGTAGGAATAACTGTTTTAACAAGCTTTTCTCTTCAAG
ATTTTCAAACTGATCTTGATAGAAATAATTCAATTGAAGAAAATGTATTGAGACTTGCAAAATTGTCTTTTGATGCTGGA
TTAGATGGATGTGTTTGTTCCCCTTGGGAGGTAAAAATGTTGAGAAATATTTATAAGAATAATTTTGAACTTATTACACC
AGGCATTAGATTAAATATTGACAGCAAAGATGATCAAAATAGAATTATGACTCCCAATGAAGCTATAGATAATGGCGCTT
CTAAGTTGGTCATTGGTAGATCAATATCAAAAGCTATAGATCCTAATAAAGCTCTAATAGAAATATTTAAATCTATTGAG
TCTGATTAA

Upstream 100 bases:

>100_bases
AATAAATCCTGATTTGGTTTTTAATTCAAAAAATGATTTGGAAGGGAAAATTTTGCAAATTGGAAAAAAAATAATTAAGA
GGTTTGAAAACTGAAAATTG

Downstream 100 bases:

>100_bases
TTTTGGATTCTTCTCCCTTAAGCAATCTTGTTATGTTTGTTCTATGTTTCCAGATTACTAGTAATGCCACAATTAAACTT
ATAAAAAAATATGAGTGCAT

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MNKRFNSEDKIILAIDGLDVSQAKLLLEKCPDIKWVKIGLELFVREGPRVIEILKGLNKKIFLDLKFHDIPNTMRAACFQ
VSKLGVDIISIHSSAGLKALKESKKASLEGAKSLGVKPPFVVGITVLTSFSLQDFQTDLDRNNSIEENVLRLAKLSFDAG
LDGCVCSPWEVKMLRNIYKNNFELITPGIRLNIDSKDDQNRIMTPNEAIDNGASKLVIGRSISKAIDPNKALIEIFKSIE
SD

Sequences:

>Translated_242_residues
MNKRFNSEDKIILAIDGLDVSQAKLLLEKCPDIKWVKIGLELFVREGPRVIEILKGLNKKIFLDLKFHDIPNTMRAACFQ
VSKLGVDIISIHSSAGLKALKESKKASLEGAKSLGVKPPFVVGITVLTSFSLQDFQTDLDRNNSIEENVLRLAKLSFDAG
LDGCVCSPWEVKMLRNIYKNNFELITPGIRLNIDSKDDQNRIMTPNEAIDNGASKLVIGRSISKAIDPNKALIEIFKSIE
SD
>Mature_242_residues
MNKRFNSEDKIILAIDGLDVSQAKLLLEKCPDIKWVKIGLELFVREGPRVIEILKGLNKKIFLDLKFHDIPNTMRAACFQ
VSKLGVDIISIHSSAGLKALKESKKASLEGAKSLGVKPPFVVGITVLTSFSLQDFQTDLDRNNSIEENVLRLAKLSFDAG
LDGCVCSPWEVKMLRNIYKNNFELITPGIRLNIDSKDDQNRIMTPNEAIDNGASKLVIGRSISKAIDPNKALIEIFKSIE
SD

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=240, Percent_Identity=34.1666666666667, Blast_Score=144, Evalue=6e-36,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_PROM0 (A3PEG2)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001091738.1
- ProteinModelPortal:   A3PEG2
- SMR:   A3PEG2
- STRING:   A3PEG2
- GeneID:   4912572
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_15141
- eggNOG:   COG0284
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- ProtClustDB:   CLSK922152
- BioCyc:   PMAR167546:P9301ORF_1542-MONOMER
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 26897; Mature: 26897

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 66-66 BINDING 16-16 BINDING 37-37 BINDING 128-128 BINDING 190-190 BINDING 199-199 BINDING 219-219 BINDING 220-220

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKRFNSEDKIILAIDGLDVSQAKLLLEKCPDIKWVKIGLELFVREGPRVIEILKGLNKK
CCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEEHHHHCCCCCHHHHHHHCCCCE
IFLDLKFHDIPNTMRAACFQVSKLGVDIISIHSSAGLKALKESKKASLEGAKSLGVKPPF
EEEEEEECCCCHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCH
VVGITVLTSFSLQDFQTDLDRNNSIEENVLRLAKLSFDAGLDGCVCSPWEVKMLRNIYKN
HHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHC
NFELITPGIRLNIDSKDDQNRIMTPNEAIDNGASKLVIGRSISKAIDPNKALIEIFKSIE
CCEEECCCEEEEECCCCCCCCCCCCCHHHHCCCCEEEECCHHHHHCCCCHHHHHHHHHHC
SD
CC
>Mature Secondary Structure
MNKRFNSEDKIILAIDGLDVSQAKLLLEKCPDIKWVKIGLELFVREGPRVIEILKGLNKK
CCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEEHHHHCCCCCHHHHHHHCCCCE
IFLDLKFHDIPNTMRAACFQVSKLGVDIISIHSSAGLKALKESKKASLEGAKSLGVKPPF
EEEEEEECCCCHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCH
VVGITVLTSFSLQDFQTDLDRNNSIEENVLRLAKLSFDAGLDGCVCSPWEVKMLRNIYKN
HHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHC
NFELITPGIRLNIDSKDDQNRIMTPNEAIDNGASKLVIGRSISKAIDPNKALIEIFKSIE
CCEEECCCEEEEECCCCCCCCCCCCCHHHHCCCCEEEECCHHHHHCCCCHHHHHHHHHHC
SD
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA