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The map label for this gene is glgX [H]

Identifier: 126696846

GI number: 126696846

Start: 1275239

End: 1277272

Strand: Direct

Name: glgX [H]

Synonym: P9301_15081

Alternate gene names: 126696846

Gene position: 1275239-1277272 (Clockwise)

Preceding gene: 126696841

Following gene: 126696847

Centisome position: 77.67

GC content: 31.51

Gene sequence:

>2034_bases
GTGATTCATCTCAATAAAGGTAAACCATTTCCTTTAGGGAGTTCTCTAACTTCACAAGGGGTAAATTTTTCCTTAATAGC
CACAAATGCTGAATATGTAGAAATATTATTGTTTGAGAGAGAAGACTCTATTTTCCCAAAAAGCATATTCAAATTAGATC
AGACTCACAATAAAGGTCCTTACTGGCATGCGGAAATAAAAAATCTAAATGAAGGTTGCATTTATGCTTTTAGAGTGAAA
CAAAAAAATAATGGAATTAATAATAACTATGAAAAAAAAGTATTACTTGATCCATGTTCAAGGGGTATAACCGGATGGGG
GAGTTATAAAAGAGAAAATTCATTAAAAACGCATGAAAATACTAATTCTTGTCTTAAAAGCGTTGTTTGCGATAGGAAAT
TATTTAATTTTAAGGATTATCCAAGACCAAAACATTCTTGGGAAGAAACAATTATTTATGAACTTCACATCAAAGCTTTT
ACTGAATCAACTGATAGAGATGAAAGTTGTTTTAAGAAATTTTTAAAAAAAATTCCGTATCTCAAAGAACTTGGTATTAC
AACAATTGAATTACTTCCAATTTTTTGTTTTGATCCTACTGATGCCCCAAACGGTCTAAAAAATTTTTGGGGTTATAGTC
CAATTAATTGGTTTACTCCGCATTTTGAATATCTTTCGAATGAATCCGCCGAAGAGAATAGAGAGGAGTTTAGAAGATTT
GTAGAGGAATGTCACAAAGCAGACATTGAGGTCATCTTAGATGTTGTGTATAATCACACCTCCGAAGGTGATTCCAAAGG
TCCAGCAATATCTTGGAAAGGTATAGATGAAAATCTTTATTACTTTATTGGAAAAGATAAAAATTATCAGGACGTCTCGG
GATGTGGTAATACTATTGCAGCAAACAGAGGATTAGTTAGAAAACTAATAATTGAATCATTAAAATGTTGGGCGAGTGAA
TTAGGAGTTGATGGTTTTAGATTTGATTTAGGGATTGCCCTATCAAGAGGCGAAAATCTCTCACCACTTGATAATCCTCC
GATTTTTGAAGATATAGAATGTGAACCAGAACTTATTGATATAAAATTTATAAGTGAGCCATGGGATTGTGGTGGTTTAT
ATAAATTAGGGGATTTTCCATCTAAGAATACTTTCACTTGGAATGGTCATTTTAGAGATGATTTGCGAAGATTTTGGAAG
GGGGATAAAGATACAGCTTGGAATATGAGCGATAAAATCAAAGGCTCTCCATCGATTTATAAAGAAGATAATATTTTCCC
AAAATCAATAAACTTTATTACTTCGCATGATGGATTTACTCTGAAAGATTTAGTAACTTTCAATAGAAAACATAATTTTG
CCAACAGAGAGCAAAACAGAGATGGTGATAACCATAACAATTCTTGGAATCATGGGGAGGAGGGACCAACTACAAACTTA
TTAATTAATGATTTAAGAAAAAGACAACAAAAAAATCTTATTCTTAGTTTACTTATCTCTAGAGGTGTTCCAATGATACT
TATGGGTGATGAGATAGGAAGATCACAAGGCGGCAACAATAATTCTTGGTGTCAAAATAATTTATTGGGCTGGATGAATT
GGGAACATGGTCATCAAGATTTGGAATTATTAGAATATTTTAAATACGTTATAAAAATCAGAAAAGAACTAATAAACATT
TTTAATCCACCATTCTTCCCTAAGAATAAAACTAATGAAAATATTCCAACATATCATTGGCATGGAACAAAATTAGATAA
TCCCGATTGGAGTAGTTGGTCTCACACAGTTGCTTTTAGCATTAACAAAGGAATTACTAATCCACTGGTCTGGATCGGTT
TAAATGCATATTCAAAAAGTATCAATTTCCCCTTGCCGAAATGTAAATATAATTGGTTAAAAGTTATTGACACTAGCATG
TCTAAAATTTTTGAACCCTTAACTATTGATGAAAAATTTGTTTCAATAAAGAGTAGAAGCTCTTTATTAATAATTTCAGA
AGAAGTATTTGGGGCAAAAAATAATTTATTCTAA

Upstream 100 bases:

>100_bases
CTTAAATTTGAGTTTTTTTTTCAATTTTGCATCTATTATTCAAATATCAGTAATTTAAATAAATTATCTCAATATTTAAA
TAATCAATGATTAAGAGAAA

Downstream 100 bases:

>100_bases
AGCGGGCGGCGGGAATCGAACCCGCATCTTCAGCTTGGAAGGCTGAGGTTTTACCACTAAACCACGCCCGCATTAAGTAA
TAGAATTACCATTGCAATAA

Product: putative isoamylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 677; Mature: 677

Protein sequence:

>677_residues
MIHLNKGKPFPLGSSLTSQGVNFSLIATNAEYVEILLFEREDSIFPKSIFKLDQTHNKGPYWHAEIKNLNEGCIYAFRVK
QKNNGINNNYEKKVLLDPCSRGITGWGSYKRENSLKTHENTNSCLKSVVCDRKLFNFKDYPRPKHSWEETIIYELHIKAF
TESTDRDESCFKKFLKKIPYLKELGITTIELLPIFCFDPTDAPNGLKNFWGYSPINWFTPHFEYLSNESAEENREEFRRF
VEECHKADIEVILDVVYNHTSEGDSKGPAISWKGIDENLYYFIGKDKNYQDVSGCGNTIAANRGLVRKLIIESLKCWASE
LGVDGFRFDLGIALSRGENLSPLDNPPIFEDIECEPELIDIKFISEPWDCGGLYKLGDFPSKNTFTWNGHFRDDLRRFWK
GDKDTAWNMSDKIKGSPSIYKEDNIFPKSINFITSHDGFTLKDLVTFNRKHNFANREQNRDGDNHNNSWNHGEEGPTTNL
LINDLRKRQQKNLILSLLISRGVPMILMGDEIGRSQGGNNNSWCQNNLLGWMNWEHGHQDLELLEYFKYVIKIRKELINI
FNPPFFPKNKTNENIPTYHWHGTKLDNPDWSSWSHTVAFSINKGITNPLVWIGLNAYSKSINFPLPKCKYNWLKVIDTSM
SKIFEPLTIDEKFVSIKSRSSLLIISEEVFGAKNNLF

Sequences:

>Translated_677_residues
MIHLNKGKPFPLGSSLTSQGVNFSLIATNAEYVEILLFEREDSIFPKSIFKLDQTHNKGPYWHAEIKNLNEGCIYAFRVK
QKNNGINNNYEKKVLLDPCSRGITGWGSYKRENSLKTHENTNSCLKSVVCDRKLFNFKDYPRPKHSWEETIIYELHIKAF
TESTDRDESCFKKFLKKIPYLKELGITTIELLPIFCFDPTDAPNGLKNFWGYSPINWFTPHFEYLSNESAEENREEFRRF
VEECHKADIEVILDVVYNHTSEGDSKGPAISWKGIDENLYYFIGKDKNYQDVSGCGNTIAANRGLVRKLIIESLKCWASE
LGVDGFRFDLGIALSRGENLSPLDNPPIFEDIECEPELIDIKFISEPWDCGGLYKLGDFPSKNTFTWNGHFRDDLRRFWK
GDKDTAWNMSDKIKGSPSIYKEDNIFPKSINFITSHDGFTLKDLVTFNRKHNFANREQNRDGDNHNNSWNHGEEGPTTNL
LINDLRKRQQKNLILSLLISRGVPMILMGDEIGRSQGGNNNSWCQNNLLGWMNWEHGHQDLELLEYFKYVIKIRKELINI
FNPPFFPKNKTNENIPTYHWHGTKLDNPDWSSWSHTVAFSINKGITNPLVWIGLNAYSKSINFPLPKCKYNWLKVIDTSM
SKIFEPLTIDEKFVSIKSRSSLLIISEEVFGAKNNLF
>Mature_677_residues
MIHLNKGKPFPLGSSLTSQGVNFSLIATNAEYVEILLFEREDSIFPKSIFKLDQTHNKGPYWHAEIKNLNEGCIYAFRVK
QKNNGINNNYEKKVLLDPCSRGITGWGSYKRENSLKTHENTNSCLKSVVCDRKLFNFKDYPRPKHSWEETIIYELHIKAF
TESTDRDESCFKKFLKKIPYLKELGITTIELLPIFCFDPTDAPNGLKNFWGYSPINWFTPHFEYLSNESAEENREEFRRF
VEECHKADIEVILDVVYNHTSEGDSKGPAISWKGIDENLYYFIGKDKNYQDVSGCGNTIAANRGLVRKLIIESLKCWASE
LGVDGFRFDLGIALSRGENLSPLDNPPIFEDIECEPELIDIKFISEPWDCGGLYKLGDFPSKNTFTWNGHFRDDLRRFWK
GDKDTAWNMSDKIKGSPSIYKEDNIFPKSINFITSHDGFTLKDLVTFNRKHNFANREQNRDGDNHNNSWNHGEEGPTTNL
LINDLRKRQQKNLILSLLISRGVPMILMGDEIGRSQGGNNNSWCQNNLLGWMNWEHGHQDLELLEYFKYVIKIRKELINI
FNPPFFPKNKTNENIPTYHWHGTKLDNPDWSSWSHTVAFSINKGITNPLVWIGLNAYSKSINFPLPKCKYNWLKVIDTSM
SKIFEPLTIDEKFVSIKSRSSLLIISEEVFGAKNNLF

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=578, Percent_Identity=37.8892733564014, Blast_Score=355, Evalue=4e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 78340; Mature: 78340

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHLNKGKPFPLGSSLTSQGVNFSLIATNAEYVEILLFEREDSIFPKSIFKLDQTHNKGP
CEECCCCCCCCCCCCHHHCCCCEEEEECCCCEEEEEEEECCCCCCHHHHHHHHCCCCCCC
YWHAEIKNLNEGCIYAFRVKQKNNGINNNYEKKVLLDPCSRGITGWGSYKRENSLKTHEN
EEEHHHCCCCCCEEEEEEEEECCCCCCCCCCCEEEECHHHCCCCCCCCCCCCCCCCCCCC
TNSCLKSVVCDRKLFNFKDYPRPKHSWEETIIYELHIKAFTESTDRDESCFKKFLKKIPY
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHCCC
LKELGITTIELLPIFCFDPTDAPNGLKNFWGYSPINWFTPHFEYLSNESAEENREEFRRF
HHHCCCEEEEEEEEEEECCCCCCCHHHHHCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHH
VEECHKADIEVILDVVYNHTSEGDSKGPAISWKGIDENLYYFIGKDKNYQDVSGCGNTIA
HHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCHHHCCCCCHH
ANRGLVRKLIIESLKCWASELGVDGFRFDLGIALSRGENLSPLDNPPIFEDIECEPELID
HCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEE
IKFISEPWDCGGLYKLGDFPSKNTFTWNGHFRDDLRRFWKGDKDTAWNMSDKIKGSPSIY
EEEECCCCCCCCEEECCCCCCCCEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
KEDNIFPKSINFITSHDGFTLKDLVTFNRKHNFANREQNRDGDNHNNSWNHGEEGPTTNL
CCCCCCCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
LINDLRKRQQKNLILSLLISRGVPMILMGDEIGRSQGGNNNSWCQNNLLGWMNWEHGHQD
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHCCCCCCCCCCCHHHCCCCCCCCCCCCHH
LELLEYFKYVIKIRKELINIFNPPFFPKNKTNENIPTYHWHGTKLDNPDWSSWSHTVAFS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEE
INKGITNPLVWIGLNAYSKSINFPLPKCKYNWLKVIDTSMSKIFEPLTIDEKFVSIKSRS
ECCCCCCCEEEEEEHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHEECCC
SLLIISEEVFGAKNNLF
CEEEEEHHHCCCCCCCC
>Mature Secondary Structure
MIHLNKGKPFPLGSSLTSQGVNFSLIATNAEYVEILLFEREDSIFPKSIFKLDQTHNKGP
CEECCCCCCCCCCCCHHHCCCCEEEEECCCCEEEEEEEECCCCCCHHHHHHHHCCCCCCC
YWHAEIKNLNEGCIYAFRVKQKNNGINNNYEKKVLLDPCSRGITGWGSYKRENSLKTHEN
EEEHHHCCCCCCEEEEEEEEECCCCCCCCCCCEEEECHHHCCCCCCCCCCCCCCCCCCCC
TNSCLKSVVCDRKLFNFKDYPRPKHSWEETIIYELHIKAFTESTDRDESCFKKFLKKIPY
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHCCC
LKELGITTIELLPIFCFDPTDAPNGLKNFWGYSPINWFTPHFEYLSNESAEENREEFRRF
HHHCCCEEEEEEEEEEECCCCCCCHHHHHCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHH
VEECHKADIEVILDVVYNHTSEGDSKGPAISWKGIDENLYYFIGKDKNYQDVSGCGNTIA
HHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCHHHCCCCCHH
ANRGLVRKLIIESLKCWASELGVDGFRFDLGIALSRGENLSPLDNPPIFEDIECEPELID
HCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEE
IKFISEPWDCGGLYKLGDFPSKNTFTWNGHFRDDLRRFWKGDKDTAWNMSDKIKGSPSIY
EEEECCCCCCCCEEECCCCCCCCEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
KEDNIFPKSINFITSHDGFTLKDLVTFNRKHNFANREQNRDGDNHNNSWNHGEEGPTTNL
CCCCCCCCCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
LINDLRKRQQKNLILSLLISRGVPMILMGDEIGRSQGGNNNSWCQNNLLGWMNWEHGHQD
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHCCCCCCCCCCCHHHCCCCCCCCCCCCHH
LELLEYFKYVIKIRKELINIFNPPFFPKNKTNENIPTYHWHGTKLDNPDWSSWSHTVAFS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEE
INKGITNPLVWIGLNAYSKSINFPLPKCKYNWLKVIDTSMSKIFEPLTIDEKFVSIKSRS
ECCCCCCCEEEEEEHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHEECCC
SLLIISEEVFGAKNNLF
CEEEEEHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]