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The map label for this gene is argF [H]

Identifier: 126696786

GI number: 126696786

Start: 1219595

End: 1220521

Strand: Reverse

Name: argF [H]

Synonym: P9301_14481

Alternate gene names: 126696786

Gene position: 1220521-1219595 (Counterclockwise)

Preceding gene: 126696787

Following gene: 126696785

Centisome position: 74.34

GC content: 32.79

Gene sequence:

>927_bases
ATGTTAAAGCCCCATAAGCTTTCTAATGATAATTTTTTATCATGCCTTGATATATCTGCAGATGAAGTTCATCATATTTT
AGAAGTTGCTAAAAACTTTAAGAATGAAGACCTAGAGCTAAAACTTAAAAATAAAGTTTTAGGATTGATTTTTGATAAAT
CTTCAACTCGTACAAGAGTAAGCTTCCAAGTTGCTATGTCGAGATTAGGAGGAACAACTATTGATCTTAACCCTAACACA
TCACAAATAGGAAGAGGTGAGGCAATTAAAGATACAGCAAGAGTCTTGAGTAGATATTGTGATGTAATTGCAATAAGGAC
ATTTAAGCATTCAGATTTAGAGGAGTATGCAAAATGGTCCACAAAGCCAATTATCAATGCCCTCACAGACTTCGAACATC
CTTGTCAAGCTCTAGCAGATTTTTTGACTATTAAGGAAGAATACTTTGATTTTGAAAATGTGGTTTTGACATTTGTCGGA
GATGGTAATAATGTCGCTAATTCTCTTATTTTATGTGGAGCTTTATTAGGTGTTGAAGTTAGAATTGCCTGCCCTAAAGG
TTATGAACCAGATTCATTGGTGATTAATCAAGCCAGAGAGATTTATAAGAATAAGAATTTATTAAAAATTTCTAATGATC
CTTTTTCTGCTGTTTCAGGAGCGAATGTACTTTATACAGATGTTTGGTCATCTATGGGGGAAGAAAAACAAAAAGAAGAG
AAAGATAAAGATTTTAATGGATTTTCTATTGATGAAAATCTATTGAGTAAGGCAGAAAAAGATGCAATTATTCTTCATTG
CCTACCAGCTTATAGAGGTAAAGAGATTACTGAAAAAGTGATGGAAAGTAAAAATAGTAGAATTTTTAATCAGGCAGAGA
ATAGGATGCACGCTCAACAAGCACTTTTAGCATGTCTTCTTGCATAA

Upstream 100 bases:

>100_bases
ATTCTTGAAGAGGAAGTTATAGAAGGTGAAGATTTGAAAGCTCTACTTGCGGAAAGTAAAATGCCAACATAGTAGAATTG
AGACTTTATCAAAGATATTT

Downstream 100 bases:

>100_bases
AAAAAACTTGCTTTTTACCTCGTAAAAAGGTACAAATGTATTAGTAATTAAATTTTTCTGTGATAACTTCTTCAGGCGAT
AAACTTACAGTTGCTCAAAG

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MLKPHKLSNDNFLSCLDISADEVHHILEVAKNFKNEDLELKLKNKVLGLIFDKSSTRTRVSFQVAMSRLGGTTIDLNPNT
SQIGRGEAIKDTARVLSRYCDVIAIRTFKHSDLEEYAKWSTKPIINALTDFEHPCQALADFLTIKEEYFDFENVVLTFVG
DGNNVANSLILCGALLGVEVRIACPKGYEPDSLVINQAREIYKNKNLLKISNDPFSAVSGANVLYTDVWSSMGEEKQKEE
KDKDFNGFSIDENLLSKAEKDAIILHCLPAYRGKEITEKVMESKNSRIFNQAENRMHAQQALLACLLA

Sequences:

>Translated_308_residues
MLKPHKLSNDNFLSCLDISADEVHHILEVAKNFKNEDLELKLKNKVLGLIFDKSSTRTRVSFQVAMSRLGGTTIDLNPNT
SQIGRGEAIKDTARVLSRYCDVIAIRTFKHSDLEEYAKWSTKPIINALTDFEHPCQALADFLTIKEEYFDFENVVLTFVG
DGNNVANSLILCGALLGVEVRIACPKGYEPDSLVINQAREIYKNKNLLKISNDPFSAVSGANVLYTDVWSSMGEEKQKEE
KDKDFNGFSIDENLLSKAEKDAIILHCLPAYRGKEITEKVMESKNSRIFNQAENRMHAQQALLACLLA
>Mature_308_residues
MLKPHKLSNDNFLSCLDISADEVHHILEVAKNFKNEDLELKLKNKVLGLIFDKSSTRTRVSFQVAMSRLGGTTIDLNPNT
SQIGRGEAIKDTARVLSRYCDVIAIRTFKHSDLEEYAKWSTKPIINALTDFEHPCQALADFLTIKEEYFDFENVVLTFVG
DGNNVANSLILCGALLGVEVRIACPKGYEPDSLVINQAREIYKNKNLLKISNDPFSAVSGANVLYTDVWSSMGEEKQKEE
KDKDFNGFSIDENLLSKAEKDAIILHCLPAYRGKEITEKVMESKNSRIFNQAENRMHAQQALLACLLA

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=309, Percent_Identity=37.8640776699029, Blast_Score=222, Evalue=4e-58,
Organism=Homo sapiens, GI18105007, Length=316, Percent_Identity=31.0126582278481, Blast_Score=132, Evalue=4e-31,
Organism=Escherichia coli, GI1790703, Length=328, Percent_Identity=35.0609756097561, Blast_Score=195, Evalue=3e-51,
Organism=Escherichia coli, GI1786469, Length=330, Percent_Identity=35.7575757575758, Blast_Score=188, Evalue=3e-49,
Organism=Escherichia coli, GI48994908, Length=360, Percent_Identity=25, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI2367364, Length=327, Percent_Identity=30.5810397553517, Blast_Score=93, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI193204318, Length=335, Percent_Identity=26.865671641791, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6322373, Length=313, Percent_Identity=33.8658146964856, Blast_Score=177, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6322331, Length=310, Percent_Identity=30.6451612903226, Blast_Score=123, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24642586, Length=315, Percent_Identity=28.8888888888889, Blast_Score=119, Evalue=2e-27,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 34594; Mature: 34594

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKPHKLSNDNFLSCLDISADEVHHILEVAKNFKNEDLELKLKNKVLGLIFDKSSTRTRV
CCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEECCCCCCHHH
SFQVAMSRLGGTTIDLNPNTSQIGRGEAIKDTARVLSRYCDVIAIRTFKHSDLEEYAKWS
HHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHCC
TKPIINALTDFEHPCQALADFLTIKEEYFDFENVVLTFVGDGNNVANSLILCGALLGVEV
CHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHCCEE
RIACPKGYEPDSLVINQAREIYKNKNLLKISNDPFSAVSGANVLYTDVWSSMGEEKQKEE
EEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHCCCCCHHHHHHHHHCHHHHHHH
KDKDFNGFSIDENLLSKAEKDAIILHCLPAYRGKEITEKVMESKNSRIFNQAENRMHAQQ
HCCCCCCCCCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
ALLACLLA
HHHHHHCC
>Mature Secondary Structure
MLKPHKLSNDNFLSCLDISADEVHHILEVAKNFKNEDLELKLKNKVLGLIFDKSSTRTRV
CCCCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEECCCCCCHHH
SFQVAMSRLGGTTIDLNPNTSQIGRGEAIKDTARVLSRYCDVIAIRTFKHSDLEEYAKWS
HHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHCC
TKPIINALTDFEHPCQALADFLTIKEEYFDFENVVLTFVGDGNNVANSLILCGALLGVEV
CHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHCCEE
RIACPKGYEPDSLVINQAREIYKNKNLLKISNDPFSAVSGANVLYTDVWSSMGEEKQKEE
EEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHCCCCCHHHHHHHHHCHHHHHHH
KDKDFNGFSIDENLLSKAEKDAIILHCLPAYRGKEITEKVMESKNSRIFNQAENRMHAQQ
HCCCCCCCCCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
ALLACLLA
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]