Click here to switch to the map view.

The map label for this gene is capI [H]

Identifier: 126696737

GI number: 126696737

Start: 1167086

End: 1168120

Strand: Reverse

Name: capI [H]

Synonym: P9301_13991

Alternate gene names: 126696737

Gene position: 1168120-1167086 (Counterclockwise)

Preceding gene: 126696743

Following gene: 126696736

Centisome position: 71.15

GC content: 28.31

Gene sequence:

>1035_bases
ATGAAAAAAGATAAACCTTCAATACTGATAACAGGTGCAGCCGGATTTATTGGCTCTGCTCTTGCAATAAAACTTTTAGA
AGAAAATAATCATGTTATTGGGATTGATAATATTAATAATTATTATTCGACTTCTTTTAAAAAAGAAAGATTAAGCAATA
TTATTAAAAGCCCTAATTCAAGTAAATATTGGAATTTTCACAAAATATCTATAGAAAATAAAAATCAAATATTTAATATA
TTTGAAAAATATAGACCAAAAATTGTAGTTAATTTAGCTGCTCAAGCAGGCGTTAGGTATTCTTTGGAAAATCCAGAAGC
ATATATAAAAAGCAATTTGGTTGGTTTCTTTAATATTTTGGAAGCATGCAAATCATTTGATGTAGAAAATTTTATATATG
CTTCAAGTAGTTCTGTTTATGGCGGTAATAAAAAAGTACCTTTTAAAGAAAATGATAGCTCAAATCATCCTATAAGCTTA
TATGCAGCCACGAAAAGATCTAATGAACTAATGGCTCATTCATATAGTCATTTATATGATATCCCATCAATAGGATTAAG
ATTCTTCACTGTTTATGGCCCCTGGGGAAGGCCTGATATGGCTCCAATGATTTTTGCAAATGCAATAATGAATCAATTAC
CCATTAACATATTTAATTTTGGCAAAATGAAAAGAGATTTTACTTATATAGATGATGTTGTTGAATCTATAAAAAGATGT
TGTTTTAAACCTGCAACAGTATCAAATACTTTTGATAAATTTGATCCTGAAAATTCTTGCTCTTTTGCAAAGCATAGAAT
ATTTAACGTTGGTAACAGTAAGCCAGTGGAATTAGAAATTTTCATTGAATTACTTGAAGATGCTTTAGGCAAAAAGGCTA
TAAAGAAATATGATGAATTGCAAAAAGGAGATGTTATTTCAACCTTATCTGATACAAGTTTATTAACTAAATGGATTGAT
TTTACACCATCAACTTCTATACAAGATGGAATTCTGTATTTCGCTAAATGGTTCGAAAATTACTATGAGCATTAA

Upstream 100 bases:

>100_bases
AGCAATAATTAAAAAATATATTTCTATAAAACCACCTATATGATTTTTTTTTTAGTGATTGTAATAAATAAATTTAGTAA
ATTTTGAGTATCACAAACTT

Downstream 100 bases:

>100_bases
ATATAATTTTATTTAATTATGCAACCAAATTCCTTCCCTATATTAAAGTATTTTAAACATTAATTTTATGGATAATTCAA
ATGTAAACATTGTGAAGAAC

Product: putative nucleotide sugar epimerase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MKKDKPSILITGAAGFIGSALAIKLLEENNHVIGIDNINNYYSTSFKKERLSNIIKSPNSSKYWNFHKISIENKNQIFNI
FEKYRPKIVVNLAAQAGVRYSLENPEAYIKSNLVGFFNILEACKSFDVENFIYASSSSVYGGNKKVPFKENDSSNHPISL
YAATKRSNELMAHSYSHLYDIPSIGLRFFTVYGPWGRPDMAPMIFANAIMNQLPINIFNFGKMKRDFTYIDDVVESIKRC
CFKPATVSNTFDKFDPENSCSFAKHRIFNVGNSKPVELEIFIELLEDALGKKAIKKYDELQKGDVISTLSDTSLLTKWID
FTPSTSIQDGILYFAKWFENYYEH

Sequences:

>Translated_344_residues
MKKDKPSILITGAAGFIGSALAIKLLEENNHVIGIDNINNYYSTSFKKERLSNIIKSPNSSKYWNFHKISIENKNQIFNI
FEKYRPKIVVNLAAQAGVRYSLENPEAYIKSNLVGFFNILEACKSFDVENFIYASSSSVYGGNKKVPFKENDSSNHPISL
YAATKRSNELMAHSYSHLYDIPSIGLRFFTVYGPWGRPDMAPMIFANAIMNQLPINIFNFGKMKRDFTYIDDVVESIKRC
CFKPATVSNTFDKFDPENSCSFAKHRIFNVGNSKPVELEIFIELLEDALGKKAIKKYDELQKGDVISTLSDTSLLTKWID
FTPSTSIQDGILYFAKWFENYYEH
>Mature_344_residues
MKKDKPSILITGAAGFIGSALAIKLLEENNHVIGIDNINNYYSTSFKKERLSNIIKSPNSSKYWNFHKISIENKNQIFNI
FEKYRPKIVVNLAAQAGVRYSLENPEAYIKSNLVGFFNILEACKSFDVENFIYASSSSVYGGNKKVPFKENDSSNHPISL
YAATKRSNELMAHSYSHLYDIPSIGLRFFTVYGPWGRPDMAPMIFANAIMNQLPINIFNFGKMKRDFTYIDDVVESIKRC
CFKPATVSNTFDKFDPENSCSFAKHRIFNVGNSKPVELEIFIELLEDALGKKAIKKYDELQKGDVISTLSDTSLLTKWID
FTPSTSIQDGILYFAKWFENYYEH

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI56237023, Length=333, Percent_Identity=24.6246246246246, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI56118217, Length=333, Percent_Identity=24.6246246246246, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI189083684, Length=333, Percent_Identity=24.6246246246246, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI7657641, Length=232, Percent_Identity=30.6034482758621, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI4504031, Length=248, Percent_Identity=27.0161290322581, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI48994969, Length=351, Percent_Identity=23.9316239316239, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788353, Length=251, Percent_Identity=27.0916334661355, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1786974, Length=329, Percent_Identity=23.7082066869301, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788366, Length=161, Percent_Identity=32.2981366459627, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71982035, Length=360, Percent_Identity=23.8888888888889, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71982038, Length=357, Percent_Identity=24.0896358543417, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17539532, Length=350, Percent_Identity=24.5714285714286, Blast_Score=78, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17568069, Length=243, Percent_Identity=25.5144032921811, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI115532424, Length=247, Percent_Identity=25.9109311740891, Blast_Score=71, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=350, Percent_Identity=22.5714285714286, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI19923002, Length=341, Percent_Identity=26.099706744868, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21356223, Length=357, Percent_Identity=25.7703081232493, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 39258; Mature: 39258

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKDKPSILITGAAGFIGSALAIKLLEENNHVIGIDNINNYYSTSFKKERLSNIIKSPNS
CCCCCCCEEEEECHHHHHHHHHEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCC
SKYWNFHKISIENKNQIFNIFEKYRPKIVVNLAAQAGVRYSLENPEAYIKSNLVGFFNIL
CCCEEEEEEEECCCHHHHHHHHHCCCEEEEEEHHHCCCEEECCCCHHHHHHHHHHHHHHH
EACKSFDVENFIYASSSSVYGGNKKVPFKENDSSNHPISLYAATKRSNELMAHSYSHLYD
HHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHC
IPSIGLRFFTVYGPWGRPDMAPMIFANAIMNQLPINIFNFGKMKRDFTYIDDVVESIKRC
CCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHH
CFKPATVSNTFDKFDPENSCSFAKHRIFNVGNSKPVELEIFIELLEDALGKKAIKKYDEL
HCCCCCCCCCHHHCCCCCCHHHHHHHHEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
QKGDVISTLSDTSLLTKWIDFTPSTSIQDGILYFAKWFENYYEH
HCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKDKPSILITGAAGFIGSALAIKLLEENNHVIGIDNINNYYSTSFKKERLSNIIKSPNS
CCCCCCCEEEEECHHHHHHHHHEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCC
SKYWNFHKISIENKNQIFNIFEKYRPKIVVNLAAQAGVRYSLENPEAYIKSNLVGFFNIL
CCCEEEEEEEECCCHHHHHHHHHCCCEEEEEEHHHCCCEEECCCCHHHHHHHHHHHHHHH
EACKSFDVENFIYASSSSVYGGNKKVPFKENDSSNHPISLYAATKRSNELMAHSYSHLYD
HHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHC
IPSIGLRFFTVYGPWGRPDMAPMIFANAIMNQLPINIFNFGKMKRDFTYIDDVVESIKRC
CCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHH
CFKPATVSNTFDKFDPENSCSFAKHRIFNVGNSKPVELEIFIELLEDALGKKAIKKYDEL
HCCCCCCCCCHHHCCCCCCHHHHHHHHEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
QKGDVISTLSDTSLLTKWIDFTPSTSIQDGILYFAKWFENYYEH
HCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]