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The map label for this gene is pntB [H]

Identifier: 126696673

GI number: 126696673

Start: 1116227

End: 1117666

Strand: Reverse

Name: pntB [H]

Synonym: P9301_13351

Alternate gene names: 126696673

Gene position: 1117666-1116227 (Counterclockwise)

Preceding gene: 126696674

Following gene: 126696672

Centisome position: 68.07

GC content: 37.78

Gene sequence:

>1440_bases
ATGAATCTACCTGTAATCATTAAATTCGTTATTGACCTTCTAGCAGTACTTTTACTGGCTTTGGGAATAAAAGGATTGTC
AAAAGTAAAATCAGCAAGGGATGCCAATAGATTAGCTGCATTTGCAATGTCGCTATCAGTAGTAGGATTACTTTCTTATT
ATTTAGGCACTTCTGGAATTGCTATTCAGTCTTGGATTTGGATAATAATTGGATCAATCTTAGGTAGTTTATTCGGAGCA
ATTCTTGCAAAAAAAGTACCTATGACCTCCATGCCTGAAACAGTGGCATTGTTCAATGGTTGTGGAGGAATGTCATCACT
TTTAGTGGCCTTGGGAGTAGCTATTTTTCCTATATCTGGTGGCCAAGAAGATTTTGATTTTTTTAAGTCACTGATTAACG
AAGTCTCAATATCTGTTTCTATATTTGTTGGGGCCATAACTTTCACAGGTTCAATTGTTGCGATGGCAAAGTTGCAGGGT
TGGTTGTCAACTCCAGGATGGACTCAGAGCAAAGTTAGACATTTTGTAAATATTGTTTTTACAGTTGCTTCCTTGATAGC
CTTTTTTGATTTGATAAATGGTAATACAAGTTCTATTTGGCTTTTAGTTATAGTTTCTTCTTTATTAGGTATTGGAGTTA
CTTTGCCAATTGGTGGAGCGGATATGCCAGTCGTTATATCTTTATTAAATAGCTATTCAGGGATTGCAGCAGCAGCAGCA
GGTTTTGTTGTTGATAGTCAGCTTTTGATAGTAGCAGGCGCAATGGTTGGAGCGGCAGGTCTAATACTTACTCAAGTGAT
GTGTAAGGGTATGAATAGATCATTGGTCTCAGTTCTTTTTGGAGGATCTTTATCTGCACAAAGTACAGCCTCTTCTGGTT
CAGGAGAATATACAAATATAACTTCTTGCAGTGTTGAAGAATGCGCATTGACTTTAGAGGCAGCCAACAAGGTAATTATT
GTTCCTGGTTACGGTCTGGCAGTAGCTCAAGCTCAACATACTTTAAGGGAAGTGACAAAAAAACTAGAGCAAAATGGTAT
TGAAGTTGTTTACGCAATTCATCCTGTAGCAGGGCGGATGCCTGGACATATGAATGTACTTTTAGCAGAAGCAGATGTTC
CTTACGAACAACTTAAAGAGATGGACGTTGTAAATCCTGATTTTCCAGCAACGGATGTTGTTTTAGTTTTAGGAGCAAAT
GATGTGGTTAACCCTCAAGCTAAAAATGATAGCTCTTCTCCCTTATATGGCATGCCAGTTCTTGATGTTCAAGAAGCAAG
AACGGTATTTGTAATTAAACGTGGTATGAGTGCAGGTTACTCCGGAATAAAAAATGATTTATTTGATCTGCCAAATACCT
CAATGGTCTTTGGTGATGCAAAAAAGGTACTGAATGATTTGATTGGAGAATTAAAGGATCTTGGAGTTGGGGACAAATAA

Upstream 100 bases:

>100_bases
TTGGATTTGCTCTTTTCAACGTTGTAGGTGGTTTCTTTGTAACTGATCGAATGCTCGCGATGTTTAGTCGTAAACCATCA
AATAAGAAGTAATTTTTAAC

Downstream 100 bases:

>100_bases
TAGTATATCTTTTAGTTTTTCAGATTACGAAAAAAATAAGCCATTTCGAGAAGTCTTGCCTTGGATAGGTGGCGACTTAC
AAACTTTGAGAGATACTTTT

Product: putative nicotinamide nucleotide transhydrogenase, subunit beta

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit beta; Proton-translocating transhydrogenase NADP(H)-binding component; Pyridine nucleotide transhydrogenase subunit beta; dIII [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MNLPVIIKFVIDLLAVLLLALGIKGLSKVKSARDANRLAAFAMSLSVVGLLSYYLGTSGIAIQSWIWIIIGSILGSLFGA
ILAKKVPMTSMPETVALFNGCGGMSSLLVALGVAIFPISGGQEDFDFFKSLINEVSISVSIFVGAITFTGSIVAMAKLQG
WLSTPGWTQSKVRHFVNIVFTVASLIAFFDLINGNTSSIWLLVIVSSLLGIGVTLPIGGADMPVVISLLNSYSGIAAAAA
GFVVDSQLLIVAGAMVGAAGLILTQVMCKGMNRSLVSVLFGGSLSAQSTASSGSGEYTNITSCSVEECALTLEAANKVII
VPGYGLAVAQAQHTLREVTKKLEQNGIEVVYAIHPVAGRMPGHMNVLLAEADVPYEQLKEMDVVNPDFPATDVVLVLGAN
DVVNPQAKNDSSSPLYGMPVLDVQEARTVFVIKRGMSAGYSGIKNDLFDLPNTSMVFGDAKKVLNDLIGELKDLGVGDK

Sequences:

>Translated_479_residues
MNLPVIIKFVIDLLAVLLLALGIKGLSKVKSARDANRLAAFAMSLSVVGLLSYYLGTSGIAIQSWIWIIIGSILGSLFGA
ILAKKVPMTSMPETVALFNGCGGMSSLLVALGVAIFPISGGQEDFDFFKSLINEVSISVSIFVGAITFTGSIVAMAKLQG
WLSTPGWTQSKVRHFVNIVFTVASLIAFFDLINGNTSSIWLLVIVSSLLGIGVTLPIGGADMPVVISLLNSYSGIAAAAA
GFVVDSQLLIVAGAMVGAAGLILTQVMCKGMNRSLVSVLFGGSLSAQSTASSGSGEYTNITSCSVEECALTLEAANKVII
VPGYGLAVAQAQHTLREVTKKLEQNGIEVVYAIHPVAGRMPGHMNVLLAEADVPYEQLKEMDVVNPDFPATDVVLVLGAN
DVVNPQAKNDSSSPLYGMPVLDVQEARTVFVIKRGMSAGYSGIKNDLFDLPNTSMVFGDAKKVLNDLIGELKDLGVGDK
>Mature_479_residues
MNLPVIIKFVIDLLAVLLLALGIKGLSKVKSARDANRLAAFAMSLSVVGLLSYYLGTSGIAIQSWIWIIIGSILGSLFGA
ILAKKVPMTSMPETVALFNGCGGMSSLLVALGVAIFPISGGQEDFDFFKSLINEVSISVSIFVGAITFTGSIVAMAKLQG
WLSTPGWTQSKVRHFVNIVFTVASLIAFFDLINGNTSSIWLLVIVSSLLGIGVTLPIGGADMPVVISLLNSYSGIAAAAA
GFVVDSQLLIVAGAMVGAAGLILTQVMCKGMNRSLVSVLFGGSLSAQSTASSGSGEYTNITSCSVEECALTLEAANKVII
VPGYGLAVAQAQHTLREVTKKLEQNGIEVVYAIHPVAGRMPGHMNVLLAEADVPYEQLKEMDVVNPDFPATDVVLVLGAN
DVVNPQAKNDSSSPLYGMPVLDVQEARTVFVIKRGMSAGYSGIKNDLFDLPNTSMVFGDAKKVLNDLIGELKDLGVGDK

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG1282

COG function: function code C; NAD/NADP transhydrogenase beta subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNT beta subunit family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=469, Percent_Identity=37.953091684435, Blast_Score=286, Evalue=3e-77,
Organism=Homo sapiens, GI122939153, Length=469, Percent_Identity=37.953091684435, Blast_Score=286, Evalue=3e-77,
Organism=Escherichia coli, GI1787886, Length=468, Percent_Identity=42.3076923076923, Blast_Score=345, Evalue=5e-96,
Organism=Caenorhabditis elegans, GI17550456, Length=412, Percent_Identity=42.2330097087379, Blast_Score=301, Evalue=4e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012136
- InterPro:   IPR004003 [H]

Pfam domain/function: PF02233 PNTB [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 50195; Mature: 50195

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLPVIIKFVIDLLAVLLLALGIKGLSKVKSARDANRLAAFAMSLSVVGLLSYYLGTSGI
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AIQSWIWIIIGSILGSLFGAILAKKVPMTSMPETVALFNGCGGMSSLLVALGVAIFPISG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHEECCC
GQEDFDFFKSLINEVSISVSIFVGAITFTGSIVAMAKLQGWLSTPGWTQSKVRHFVNIVF
CCHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
TVASLIAFFDLINGNTSSIWLLVIVSSLLGIGVTLPIGGADMPVVISLLNSYSGIAAAAA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHCCHHHHHHH
GFVVDSQLLIVAGAMVGAAGLILTQVMCKGMNRSLVSVLFGGSLSAQSTASSGSGEYTNI
HHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCC
TSCSVEECALTLEAANKVIIVPGYGLAVAQAQHTLREVTKKLEQNGIEVVYAIHPVAGRM
CCCCHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHCCC
PGHMNVLLAEADVPYEQLKEMDVVNPDFPATDVVLVLGANDVVNPQAKNDSSSPLYGMPV
CCCCEEEEEECCCCHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEECCCC
LDVQEARTVFVIKRGMSAGYSGIKNDLFDLPNTSMVFGDAKKVLNDLIGELKDLGVGDK
CCHHHCCEEEEEECCCCCCCCHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNLPVIIKFVIDLLAVLLLALGIKGLSKVKSARDANRLAAFAMSLSVVGLLSYYLGTSGI
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AIQSWIWIIIGSILGSLFGAILAKKVPMTSMPETVALFNGCGGMSSLLVALGVAIFPISG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHEECCC
GQEDFDFFKSLINEVSISVSIFVGAITFTGSIVAMAKLQGWLSTPGWTQSKVRHFVNIVF
CCHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
TVASLIAFFDLINGNTSSIWLLVIVSSLLGIGVTLPIGGADMPVVISLLNSYSGIAAAAA
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHCCHHHHHHH
GFVVDSQLLIVAGAMVGAAGLILTQVMCKGMNRSLVSVLFGGSLSAQSTASSGSGEYTNI
HHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCC
TSCSVEECALTLEAANKVIIVPGYGLAVAQAQHTLREVTKKLEQNGIEVVYAIHPVAGRM
CCCCHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHCCC
PGHMNVLLAEADVPYEQLKEMDVVNPDFPATDVVLVLGANDVVNPQAKNDSSSPLYGMPV
CCCCEEEEEECCCCHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEECCCC
LDVQEARTVFVIKRGMSAGYSGIKNDLFDLPNTSMVFGDAKKVLNDLIGELKDLGVGDK
CCHHHCCEEEEEECCCCCCCCHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8075801 [H]