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The map label for this gene is gldA [C]

Identifier: 126696532

GI number: 126696532

Start: 1007312

End: 1008403

Strand: Reverse

Name: gldA [C]

Synonym: P9301_11941

Alternate gene names: 126696532

Gene position: 1008403-1007312 (Counterclockwise)

Preceding gene: 126696533

Following gene: 126696531

Centisome position: 61.42

GC content: 31.59

Gene sequence:

>1092_bases
ATGCAGTCTATCTCTCCAGAAACAATATACAGGGGAAATTCTGCTTGGGAAAAATCTTTACCTCAGATTACTAAATTAAC
TAAAAGACCATTAATTCTAGGTAGAGGGTTTCAAACGAATAATTTGAGAAATAATATTTTTAATGATTTAAAAAGTCAAA
AATTAACTGTAAATTCTGCTAATTTGCAATTTGATTGTTGTTACGAAGATATTTCAAGAGTTAAGAACATTATCTCAAAT
AATAATAATGATTCTGTTATCGCAGCTGGAGGTGGCAAAGTTCTAGACTCTGGAAAATATATAGCCGAGTCTCTTAATAT
CCCTTGTATTACAGTTCCGCTTAGCGCCTCTACATGTGCTGGTTGGACTGCCTTATCGAATATTTATACAAAGGATGGTC
AATTTATAAAAGATGTCGCATTAGGATCTTGTCCGAAAATACTAGTTTTTGATCATAAGTTTATTCAAACAGCTCCATCA
AGAACACTTGCAAGTGGCATAGCAGATGCCTTGGCAAAATGGTATGAATCCTCAATAACAAGTTCAAAAATAGACGACGG
TCTTGTTCAACAAGCAATTCAGATATCAAGAGTTTTAAGAGATCAACTTTTAATAGATGGAGGAAAAGCATTTAAGGGTC
AATTTGAAAATAATATATCTTGGCAAAATACTGTTGAAGCATGTGGACTTACAGCAGGATTAGTTGGCGGTATTGGTGGA
GAAAAATGTAGGACAGCAGCAGCACACGCTTTTCATAATGCAATTACTCAGATAATCACCCCTAATAAATTCTTACATGG
TGAGATTGTTGGGGTTGGATTATTATTGCAATTAAGACTAGAAGAAATGAAAAATAATAATAAATTAGCTGATCAATCAA
TTAAACAATTATTTGTACTCATGGAAGAATTGAATTTACCAACTACTATCGGACAACTTGGCATAAATGTTTTTGAAAAT
AATAATTTAGAAAAAATTGCTGATTTTACTTGTCGAGATAAATCTGAGATTCACTTTTTGCCTTTTGAAATTAGTAAAGG
GGAAATAATAGAAGTTATTACAAATTTTGAAAAACAAAAAATTAAAACATAA

Upstream 100 bases:

>100_bases
AGTTGATATAGATGATAATAAAAAAGTTACTATAAATATTTCTTCTGAAGAATCTTCTCAAGAGCTAGCAGGTGCTAACT
TCTAATTATTCAAAGTAAAT

Downstream 100 bases:

>100_bases
ATATTTTTTTGACTAAAAACAATTTTGAACAATTAAGTGATGTAAATATAGAATTTTTTGAAAGTGCCAAATTGTCACTA
CTAGACCCCTTAGGTCTTTA

Product: putative glycerol dehydrogenase

Products: glycerone; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MQSISPETIYRGNSAWEKSLPQITKLTKRPLILGRGFQTNNLRNNIFNDLKSQKLTVNSANLQFDCCYEDISRVKNIISN
NNNDSVIAAGGGKVLDSGKYIAESLNIPCITVPLSASTCAGWTALSNIYTKDGQFIKDVALGSCPKILVFDHKFIQTAPS
RTLASGIADALAKWYESSITSSKIDDGLVQQAIQISRVLRDQLLIDGGKAFKGQFENNISWQNTVEACGLTAGLVGGIGG
EKCRTAAAHAFHNAITQIITPNKFLHGEIVGVGLLLQLRLEEMKNNNKLADQSIKQLFVLMEELNLPTTIGQLGINVFEN
NNLEKIADFTCRDKSEIHFLPFEISKGEIIEVITNFEKQKIKT

Sequences:

>Translated_363_residues
MQSISPETIYRGNSAWEKSLPQITKLTKRPLILGRGFQTNNLRNNIFNDLKSQKLTVNSANLQFDCCYEDISRVKNIISN
NNNDSVIAAGGGKVLDSGKYIAESLNIPCITVPLSASTCAGWTALSNIYTKDGQFIKDVALGSCPKILVFDHKFIQTAPS
RTLASGIADALAKWYESSITSSKIDDGLVQQAIQISRVLRDQLLIDGGKAFKGQFENNISWQNTVEACGLTAGLVGGIGG
EKCRTAAAHAFHNAITQIITPNKFLHGEIVGVGLLLQLRLEEMKNNNKLADQSIKQLFVLMEELNLPTTIGQLGINVFEN
NNLEKIADFTCRDKSEIHFLPFEISKGEIIEVITNFEKQKIKT
>Mature_363_residues
MQSISPETIYRGNSAWEKSLPQITKLTKRPLILGRGFQTNNLRNNIFNDLKSQKLTVNSANLQFDCCYEDISRVKNIISN
NNNDSVIAAGGGKVLDSGKYIAESLNIPCITVPLSASTCAGWTALSNIYTKDGQFIKDVALGSCPKILVFDHKFIQTAPS
RTLASGIADALAKWYESSITSSKIDDGLVQQAIQISRVLRDQLLIDGGKAFKGQFENNISWQNTVEACGLTAGLVGGIGG
EKCRTAAAHAFHNAITQIITPNKFLHGEIVGVGLLLQLRLEEMKNNNKLADQSIKQLFVLMEELNLPTTIGQLGINVFEN
NNLEKIADFTCRDKSEIHFLPFEISKGEIIEVITNFEKQKIKT

Specific function: Glycerol utilization. [C]

COG id: COG0371

COG function: function code C; Glycerol dehydrogenase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082352, Length=344, Percent_Identity=29.0697674418605, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1786815, Length=299, Percent_Identity=30.7692307692308, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR016205 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.6

Molecular weight: Translated: 39921; Mature: 39921

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSISPETIYRGNSAWEKSLPQITKLTKRPLILGRGFQTNNLRNNIFNDLKSQKLTVNSA
CCCCCCCCEECCCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCEEEEECC
NLQFDCCYEDISRVKNIISNNNNDSVIAAGGGKVLDSGKYIAESLNIPCITVPLSASTCA
CEEEEEHHHHHHHHHHHHCCCCCCEEEEECCCCEECCCHHHHHHCCCCEEEEECCCCHHH
GWTALSNIYTKDGQFIKDVALGSCPKILVFDHKFIQTAPSRTLASGIADALAKWYESSIT
HHHHHHHHHCCCCCHHHHHHHCCCCEEEEECHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
SSKIDDGLVQQAIQISRVLRDQLLIDGGKAFKGQFENNISWQNTVEACGLTAGLVGGIGG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
EKCRTAAAHAFHNAITQIITPNKFLHGEIVGVGLLLQLRLEEMKNNNKLADQSIKQLFVL
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
MEELNLPTTIGQLGINVFENNNLEKIADFTCRDKSEIHFLPFEISKGEIIEVITNFEKQK
HHHCCCCHHHHHHCCEEECCCCHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHH
IKT
CCC
>Mature Secondary Structure
MQSISPETIYRGNSAWEKSLPQITKLTKRPLILGRGFQTNNLRNNIFNDLKSQKLTVNSA
CCCCCCCCEECCCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCEEEEECC
NLQFDCCYEDISRVKNIISNNNNDSVIAAGGGKVLDSGKYIAESLNIPCITVPLSASTCA
CEEEEEHHHHHHHHHHHHCCCCCCEEEEECCCCEECCCHHHHHHCCCCEEEEECCCCHHH
GWTALSNIYTKDGQFIKDVALGSCPKILVFDHKFIQTAPSRTLASGIADALAKWYESSIT
HHHHHHHHHCCCCCHHHHHHHCCCCEEEEECHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
SSKIDDGLVQQAIQISRVLRDQLLIDGGKAFKGQFENNISWQNTVEACGLTAGLVGGIGG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
EKCRTAAAHAFHNAITQIITPNKFLHGEIVGVGLLLQLRLEEMKNNNKLADQSIKQLFVL
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
MEELNLPTTIGQLGINVFENNNLEKIADFTCRDKSEIHFLPFEISKGEIIEVITNFEKQK
HHHCCCCHHHHHHCCEEECCCCHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHH
IKT
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+; NADH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.13 {NAD+}} 0.12 {NADH}} 0.06 {dihydroxyacetone}} 0.5 {glycerol}} [C]

Substrates: glycerol; NAD+

Specific reaction: glycerol + NAD+ = glycerone + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: Chelatingagents; Li+; Na+; Thiolcompounds [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]